Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Serotaxonomical Analyses of Some Streptomyces Strains Using Antibodies against Cell Envelope

균사 외피 항체를 이용한 Streptomyces 속 균주들의 혈청학적 유사성 분석

  • Jo, Sung-Kee (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Kim, Jae-Heon (Department of Microbiology and Institute of Basic Sciences, Dankook University)
  • 조성기 (단국대학교 미생물학 및 기초과학연구소) ;
  • 김재헌 (단국대학교 미생물학 및 기초과학연구소)
  • Published : 2007.06.30
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Abstract

The similarities among five strains of Streptomyces were measured by the serological methods. Antigens were prepared by dissolving cell envelope fractions in Tween 20 buffer and antisera were produced from the immunized rabbits. Immunodiffusion studies and ELISA results showed that the degree of antigen-antibody reaction was not exactly matched to the taxonomic distance; i. e. the strains of the cluster group A exhibited low level of cross-reactions each other, while relatively strong cross-reactions were observed between Streptomyces lavendulae of cluster group F and Streptomyces viridochromogenes of cluster group A.

Streptomyces 5균주 간의 유사도를 혈청학적 방법으로 측정하였다. 항원으로는 충분히 생장한 균체의 외피를 Tween 20으로 용출시킨 용액을 사용하였고 항체는 토끼를 면역시켜 얻었다. 면역확산과 ELISA 결과 항원-항체 반응의 정도가 균주간의 분류학적 유사성과 비례적으로 반영되지 않았다. 즉 군집 A에 속하는 균주들 사이의 항원-항체 반응이 낮게 측정된 반면에 군집 F의 Streptomyces Iavendulae와 군집 A의 Streptomyces viridochromogenes가 특이적으로 비교적 높게 나타났다.

Keywords

References

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