• Title/Summary/Keyword: closed culture

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Salvage of Infected Breast Implants

  • Song, Joon Ho;Kim, Young Seok;Jung, Bok Ki;Lee, Dong Won;Song, Seung Yong;Roh, Tai Suk;Lew, Dae Hyun
    • Archives of Plastic Surgery
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    • v.44 no.6
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    • pp.516-522
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    • 2017
  • Background Implant-based breast reconstruction is being performed more frequently, and implants are associated with an increased risk of infection. We reviewed the clinical features of cases of implant infection and investigated the risk factors for breast device salvage failure. Methods We retrospectively analyzed 771 patients who underwent implant-based breast reconstruction between January 2010 and December 2016. Age, body mass index, chemotherapy history, radiation exposure, and smoking history were assessed as potential risk factors for postoperative infection. We also evaluated the presence and onset of infection symptoms, wound culture pathogens, and other complications, including seroma, hematoma, and mastectomy skin necrosis. Additionally, we examined the mastectomy type, the use of acellular dermal matrix, the presence of an underlying disease such as hypertension or diabetes, and axillary node dissection. Results The total infection rate was 4.99% (58 of 1,163 cases) and the total salvage rate was 58.6% (34 of 58). The postoperative duration to closed suction drain removal was significantly different between the cellulitis and implant removal groups. Staphylococcus aureus infection was most frequently found, with methicillin resistance in 37.5% of the cases of explantation. Explantation after infection was performed more often in patients who had undergone 2-stage expander/implant reconstruction than in those who had undergone direct-to-implant reconstruction. Conclusions Preventing infection is essential in implant-based breast reconstruction. The high salvage rate argues against early implant removal. However, when infection is due to methicillin-resistant S. aureus and the patient's clinical symptoms do not improve, surgeons should consider implant removal.

Influence of Different Rice Varieties on Emission of Methane in Soil and Exudation of Carbohydrates in Rhizosphere (벼 품종별 Methane 배출과 뿌리의 탄수화물 분비 특성)

  • Lee, Kyeong-Bo;Lee, Deog-Bae;Uhm, Taik-Yong;Kim, Jong-Gu;Yoo, Chul-Hyun;Kim, Yong-Woong
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.3
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    • pp.257-264
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    • 1997
  • To find out the influence of different rice varieties on the emission of methane in the soil and the exudation of carbohydrates from root, the experiment was conducted on a fine silty, mesic family of Aeric Fluventinc Haplaquepts (Jeonbug series). The varities of rise involved one early maturing variety of Japonica type(namweon) and three mid-to-late maturing varieties of Japonica type and one mid-to-late variety of Indica${\times}$Japonica crossed. The methane flux over the rice canopy was measured according to the closed chamber method modified by Shin and the methane concentration in the soil was measured using porous cup installed in the soil. The carbohydrate exudates from root were measured under nutrient solution culture. It was found that the methane flux tended to be lower in early maturing varieties than in the mid-to-late maturing varieties, lower in indica${\times}$Japonica crossed variety than in Japonica type varieties. There was positive correlation between the number of tillers and the weight of roots of rice plant and methane flux. The correlation, however, tended to be greater between the weight of roots and methane flux. There was no significant difference in the concentration of methane in the soil under different varieties of rice, excepting one variety, Kehwa under which methane concentration was highest. In case of carbohydrate exudation, early maturing variety tended to be higher than other varieties, although the opposite was the case in methane flux.

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Effects of Growth Regulators and Culture Environment on ex vitro Rooting and Acclimatization of Apple Rootstock in vitro Propagated (기내배양 사과 대목의 기외 삽목 시 발근과 순화에 미치는 배양조건 및 생장조절물질의 효과)

  • Kwon, Soon-Il;Kim, Jeong-Hee;Kang, In-Kyu;Kim, Mok-Jong
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.133-138
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    • 2004
  • Growth of M.9 (Malus domestica Bark. cv) and M.26 (Malus domestica Bark. cv) of dwarf apple rootstock, cultured on MS agar medium in a vessel with ventilating stopper (VS) and then in vivo rooting and acclimatization under combined-treatment by some materials with IBA, were investigated. Concentration of $CO_2$ and ethylene in the vessel with VS was lower then in the vessel with non-VS. Change of temperature and humidity in the vessel with VS was repeated by light condition. Stomatal pares of tissue in the vessel with VS were immediately closed after plantlets were exposed to room humidity but those in the vessel with non-VS were opened after 20 minutes exposure to room humidity. Leaf area and chloroplast index of tissue in the vessel with VS was higher then in the vessel with non-VS. In vivo rooting ratio and acclimatization ratio of M.9 and M.26 was highest in 300mg/L IBA+3% sucrose dip-treatment among other combined- treatments.

Simultaneous Determination of Octylphenol, Nonylphenol and Bis(2-ethylhexyl)phthalate in Fish Samples Using Two Internal Standards (2가지 내부표준물질을 이용하여 어류시료 중 Octylphenol, Nonylphenol, Bis(2-ethylhexyl)phehalate의 동시정량)

  • Kim, Jong-Hun
    • Analytical Science and Technology
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    • v.14 no.3
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    • pp.244-252
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    • 2001
  • A comprehensive analytical method of endocrine distruptors[i.e., nonylphenol(NP), octylphenol(OP), bis(2-ethylhexyl)phthalate(BEHP)] is fish samples was developed using two internal standards. This method employed closed culture tube extraction with dichloromethane and solvent exchange to iso-hexane and SPE(2g) aminopropyl column, followed by determination on gas chromatograph linked to mass spectrometer(GC/MS) operated in the single ion monitoring(SIM) mode. The recoveries of nonylphenol and octyphenol in the range of $0.2{\sim}20{\mu}g/g$ using 1-phenyl decanol as one internal standard were over 75%, and recovery of bis(2-ethylhexyl)phthalate in the range of $0.4{\sim}40{\mu}g/g$ using bis(2-ethylbutyl)phthalate(BEBP) as the other internal standards was showed over 102%. The present method was applied to fish samples from Korea and UK. The range of concentrations for nonylphnol(NP) and bis(2-ethylhexyl)phthalate in Korean fish were $0.02{\sim}0.06{\mu}g/g$ in 2 samples and $0.18{\sim}2.03{\mu}g/g$ in 9 samples respectively, but bis(2-ethylhexyl)phthalate(BEHP) in UK samples was found $2.99{\mu}g/g$ in just 1 sample. But octylphenol(OP) was not dected in any samples by this method. This two internal standard method provides a more precise analytical tool to investigate endocrine disruptors in a biological matrices of limited quantity.

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A STUDY ON THE PHYSICAL PROPERTIES AND BIOLOGICAL CHARACTERISTICS OF DENTAL MAGNETIC ATTACHMENTS (치과용 자석부착장치의 물리적, 생물학적 특성에 관한 연구)

  • Lim, Yong-Sik;Kim, Yung-Soo;Kim, Chang-Whe;Kim, Yong-Ho
    • The Journal of Korean Academy of Prosthodontics
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    • v.37 no.1
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    • pp.1-22
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    • 1999
  • In order to investigate various physical and biological properties of dental magnetic attachments studies on retentive characteristics, corrosion properties, cytotoxicity of different magnetic systems for dental applications were done. For the study of retentive characteristics changes of retentive force by increasing air gap, wear properties of various attachment systems and loss of magnetic force by heat treatment were measured. Forte study of corrosion property of magnet covering metal electrochemical corrosion was done in artificial saliva and 0.9% NaCl solution between $-1,000mV_{SCE}\;and\;+1,000mV_{SCE}$. Anodic polarization curves are obtained about 6 types of samples and 2 types of solution. Corroded surfaces were examined with metallurgical microscope, scanning electron microscope and surface profilometer. For the study of cytotoxicity of magnetic attachment and its field cell growth and agar overlay test were done. The results of this study were as follows. 1. In Magnetic attachments using closed circuit retentive force at zero air gap was greater than magnets using open circuit, but decrease of retentive force by increasing air gap was also greater than open systems. 2. After 40,000 cycles of wear test all mechanical attachment resulted in varing degree of retention loss but in magnetic attachments no loss of retentive force was observed. 3. The magnetic attachment using Neodymium magnet showed early loss of retentive force about $200^{\circ}C$ but attachment using Samarium magnet showed some resistance to heat treatment and complete retention loss was observed about $500^{\circ}C$. The keeper was not influenced by heat treatment in retention. 4. In electrochemical corrosion test Dyna magnetic attachment covering metal showed the highiest corrosion resistance and Shiner magnet covering metal showed the least corrosion resistance and examination of corroded surface with metallurgical microscope, scanning electron micro-scope and surface profilometer also showed same results with anodic polarization corves. 5. The result of cell culture tests on the magnet covered with metal showed least recognizable cytotoxicity.

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Multi-Cultural Society and Social Distance for Foreigners in Korean Society (다문화사회와 외국인에 대한 사회적 거리)

  • Lee, Myoung-Jin;Choi, Yu-Jung;Choi, Set-Byol
    • Survey Research
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    • v.11 no.1
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    • pp.63-85
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    • 2010
  • This study defines the substance and multi-dimension of emotional reactions which Koreans have toward foreigners to find the starting point of change in values which is an inevitable task in this multi-cultural society. The results indicate that the Bogardus scale which has been used to measure the social distance toward the minority race is found to have limitations in explaining the closed attitude of Koreans toward 'nation' and 'kinship through marriage'. To supplement such limitations, exploration on attitudes toward foreigners from different native places is performed based on the 'evaluation', 'power' and 'activity' dimensions of the Affective Control Theory. As a result, Americans are highly evaluated in all three dimensions while Japanese are evaluated low in the 'evaluation' dimension and high in the 'power' and 'activity' dimensions. North Korean defectors and ethnic Koreans from China (the Chosun race) are high in evaluation but low in other dimensions. West Asians are evaluated low in all three dimensions. By comprehending the influencing factors and the relative influence of social distance, it proves that the 'evaluation' dimension is the common denominator in all groups while 'power' dimension toward Japanese and 'activity' dimensions toward Chinese and West Asians influence social distance. All foreigners excluding Americans receive closer social distance when having higher education level. Moreover, American women and older North Korean defectors receive closer social distance.

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Initial Development of Forest Structure and Understory Vegetation after Clear-cut in Pinus densiflora Forest in Southern Gangwon-do Province (강원도 남부 지역에서 소나무림 개벌 후 초기 임분 구조 및 하층식생 발달)

  • Bae, Kwan Ho;Kim, Jun Soo;Lee, Change Seok;Cho, Hyun Je;Lee, Ho Young;Cho, Yong Chan
    • Journal of Korean Society of Forest Science
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    • v.103 no.1
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    • pp.23-29
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    • 2014
  • Open- to closed canopy stage and it's ecological characteristics in vegetation succession are commonly described, but poorly understood in Korea. Vegetation development on structure, environment and understory abundance were studied for 16 yr in post-clearcut Pinus densiflora forests in the southern Gangwon-do province by applying space-for-time approach. We sampled 210 plots (10 for structure and 200 for understory) for four seral stages (1yr, 3yr, 10yr and 16yr). After clear-cut, mean stem density increased gradually to $5,714{\pm}645$ stems/ha after 16 years and mean basal area was also from $5.5{\pm}0.7m^2/ha$ after 10 years and doubled at $10.0{\pm}1.6m^2/ha$ in 16 years. Woody debris and bared soil on the forest floor peaked at 11--- after 10 years and at 10.3--- after 3 years, respectively. In understory mean cover declined with all growth form groups following succession, but in richness, forb specie increased with structural development during 16 years. Our study suggested that overstory development did not suppressed whole understory properties especially in richness, thus appeared to act as a filter selectively constraining the understory characteristics. However only long-term studies are essential for elucidating patterns and processes that cannot be inferred form short-term or space-for-time researches. Strong negative relationship between overstory and understory characteristics in conventional models surely reexamined.

Modeling Nutrient Uptake of Cucumber Plant Based on EC and Nutrient Solution Uptake in Closed Perlite Culture (순환식 펄라이트재배에서 EC와 양액흡수량을 이용한 오이 양분흡수 모델링)

  • 김형준;우영회;김완순;조삼증;남윤일
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2001.04b
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    • pp.75-76
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    • 2001
  • 순환식 펄라이트재배에서 배액 재사용을 위한 양분흡수 모델링을 작성하고자 EC 처리(1.5, 1.8, 2.1, 2.4, 2.7 dSㆍm-1)를 수행하였다. 생육 중기까지 EC 수준에 따른 양액흡수량은 차이가 없었지만 중기 이후 EC가 높을수록 흡수량이 감소되는 경항을 보였다(Fig. 1). NO$_3$-N, P 및 K의 흡수량은 생육기간 동안 처리간 차이를 유지하였는데 N과 K는 생육 중기 이후 일정 수준을 유지하였으나 P는 생육기간 동안 다소 증가되는 경향을 보였다. S의 흡수량은 생육 중기 이후 모든 처리에서 급격한 감소를 보였으며 생육 후기에는 처리간에 차이가 없었다(Fig. 2). 오이의 무기이온 흡수율에서와 같이 흡수량에서도 EC간 차이를 보여 EC를 무기이온 흡수량을 추정하는 요소로 이용할 수 있을 것으로 생각되었다. 무기이온 흡수량은 모든 EC 처리간에 생육 초기에는 차이를 보이지 않았으나 생육중기 이후에는 뚜렷한 차이를 보인 후 생육 후기의 높은 농도에서 그 차이가 다소 감소되는 경향을 보였다. 단위일사량에 따른 양액흡수량과 EC를 주된 변수로 한 오이의 이온 흡수량 예측 회귀식을 작성하였는데 모든 무기이온 흡수량 추정식의 상관계수는 S를 제외한 모든 이온에서 높게 나타났는데 특히 N, P, K 및 Ca에서 높았다. S이온에서의 상관계수는 0.47로 낮게 나타났으나 각 이온들의 회귀식에 대한 상관계수는 모두 1% 수준에서 유의성을 보여 위의 모델식을 순환식 양액재배에서 무기이온 추정식으로 사용이 가능할 것으로 생각되었다(Table 1). 이를 이용한 실측치와의 비교는 신뢰구간 1%내에서 높은 정의상관을 보여 실제적인 적용이 가능할 것으로 생각되었다(Fig 3)..ble 3D)를 바탕으로 MPEG-4 시스템의 특징들을 수용하여 구성되고 BIFS와 일대일로 대응된다. 반면에 XMT-0는 멀티미디어 문서를 웹문서로 표현하는 SMIL 2.0 을 그 기반으로 하였기에 MPEG-4 시스템의 특징보다는 컨텐츠를 저작하는 제작자의 초점에 맞추어 개발된 형태이다. XMT를 이용하여 컨텐츠를 저작하기 위해서는 사용자 인터페이스를 통해 입력되는 저작 정보들을 손쉽게 저장하고 조작할 수 있으며, 또한 XMT 파일 형태로 출력하기 위한 API 가 필요하다. 이에, 본 논문에서는 XMT 형태의 중간 자료형으로의 저장 및 조작을 위하여 XML 에서 표준 인터페이스로 사용하고 있는 DOM(Document Object Model)을 기반으로 하여 XMT 문법에 적합하게 API를 정의하였으며, 또한, XMT 파일을 생성하기 위한 API를 구현하였다. 본 논문에서 제공된 API는 객체기반 제작/편집 도구에 응용되어 다양한 멀티미디어 컨텐츠 제작에 사용되었다.x factorization (NMF), generative topographic mapping (GTM)의 구조와 학습 및 추론알고리즘을소개하고 이를 DNA칩 데이터 분석 평가 대회인 CAMDA-2000과 CAMDA-2001에서 사용된cancer diagnosis 문제와 gene-drug dependency analysis 문제에 적용한 결과를 살펴본다.0$\mu$M이 적당하며, 초기배발달을 유기할 때의 효과적인 cysteamine의 농도는 25~50$\mu$M인 것으로 판단된다.N)A(N)/N을 제시하였다(A(N)=N에 대한 A값). 위의 실험식을 사용하여 헝가리산 Zempleni 시료(15%

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Evaluation of Purification Capacity of Vegetable Lactobacillus fermentum Culture System in Closed Environmental Waste Water (식물성 유산균 혼합물을 활용한 환경 폐수의 정화능력 평가)

  • Lee, Deuk Sik;Kim, Nam Kyun;Shim, Sooyong;Lee, Dong Jin;Yoon, Won Byong
    • Food Engineering Progress
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    • v.15 no.1
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    • pp.22-27
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    • 2011
  • Changes in total nitrogen (T.N.) and total phosphate (T.P.) content in environmental waste water upon the reaction of biological purifying reagents were measured and the reaction rate was evaluated. The purification capacity of two biological purifying reagents composed of vegetable Lactobacillus fermentum (V.L.F.), Saccharomyces cerevisiae(S.C), and Bacillus subtilis(B.S.) were evaluated and compared with that of commercial water purification system operating by local government. After 18days of reaction, the mixture of V.L.F. and S.C. showed dramatic decrease of T.N.(36.21% of the initial value). The mixture of V.L.F., S.C., and B.S. showed faster reaction rate to decrease T.P. compared with that of the mixture of V.L.F. and S.C. The reaction constant of mixture of V.L.F. and S.C. was estimated to be 0.178 $day^{-1}$ by the curve fitting of the data of changes in T.N. during the reaction.

The origin, development, philosophy and reality awareness of Soron School(少論學派) (소론학파(少論學派)의 연원과 전개, 철학과 현실인식)

  • Kim, Jong Soo
    • The Journal of Korean Philosophical History
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    • no.32
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    • pp.113-159
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    • 2011
  • The self-segmentation of Seoin(西人) School and the appearance of Soron(少論) School in the 17th century Joseon society has an important meaning in the political history. Soron School(少論學派) had quite different line in their thought and public position from the Noron(老論) School represented by Song Shi-yeol. With such thought and position, Soron School added a vitality to the barren climate of Joseon intellectual society and soon became an important school of it. Especially, the senior leaders of early Soron School shared the culture of discussion and dialogue which was represented by the phrase, "the forest of debate and the hill of argument". Accordingly, Soron School could form an unique academic tradition which was different from other schools. For instance, Park Se-dang(朴世堂), Nam Gu-man(南九萬), Yoon Jeung(尹拯) and Park Se-chae(朴世采) had relatively flexible Conception of Heresy; which was in the same context with the academic position of Soron School that allowed diverse approaches to truth each as the path of justice. Furthermore, the leaders of Soron School continued dialogue and discussion on the wide range of current issues. They deeply sympathized with the Tangpyeong-ron(蕩平論), which was to appoint important government positions equally from multiple schools. They thought that the Tangpyeong-ron(蕩平論) was to avoid the harmful influence of conflicts between schools, the Bungdang(朋黨), and tried to tune and compromise the different opinions of different schools. In fact, the "Hwanggeuk Tangpyeong-ron" (皇極蕩平論) submitted by Park Se-chae was the summarization of the discussions shared by the Soron School leaders. Consequently, the seniors of Soron School practiced their philosophy that "the true academic practice is the communication" by keeping the dialogue with other schools while respecting the difference of each other, rather than staying comfortably in the closed academic fence of uniformity. Such communicative academic practice adopting discussion and dialogue had been succeeded to the second generation of Soron School and it remained as an unique academic tradition of Soron School.