• Journal of Genetic Medicine
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• v.10 no.1
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• pp.33-37
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• 2013

Purpose: Myotonic dystrophy 1 (DM1, OMIM 160900) is an autosomal-dominant muscular disorder caused by an expansion of CTG repeats in the 3' UTR of the DMPK gene. Variable expansions of CTG repeats preclude the accurate determination of repeat size. We tried to show the clinical and analytical validity of the application of Southern blotting after long-range PCR was demonstrated in Korean DM1 patients. Materials and Methods: The Southern blotting of long-range PCR was applied to 1,231 cases with clinical suspicion of DM1, between 2000 and 2011. PCR was performed using genomic DNA with forward 5'-CAGTTCACAACCGCTCCGAGC-3' and reverse 5'-CGTGGAGGATGGAACACGGAC-3' primers. Subsequently, the PCR fragments were subjected to gel electrophoresis, capillary transfer to a nylon membrane, hybridization with a labeled (CAG)10 probe. The correlation between clinical manifestations and the CTG repeat expansions were analyzed. Results: Among a total of 1,231 tested cases, 642 individuals were diagnosed with DM1 and the range of the detected expansion was 50 to 2,500 repeats; fourteen cases with mild DM1 ($75{\pm}14$ repeats), 602 cases with classical DM1 ($314{\pm}143$ repeats), and 26 cases with congenital DM1 ($1,219{\pm}402$ repeats). The positive and negative predictive values were 100%. The age at test requested and the CTG repeat numbers were inversely correlated (R=-0.444, P<0.01). Conclusion: This study indicates that Southern blotting after long-range PCR is a reliable diagnostic method DM1.

• Research in Plant Disease
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• v.13 no.3
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• pp.145-151
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• 2007

Xanthomonas axonopodis pv. glycines is the causal agent of bacterial pustule of soybean(Glycine max. (L.) Merr), which is one of the most prevalent bacterial diseases in Korea. In this study, Polymerase Chain Reaction (PCR) assay was applied to detect Xanthomonas axonopodis pv. glycines and to survey on seed contamination in 36 soybean cultivars of Korea. And we have to compare PCR assay with dilution-plating assay of detection and identification. We confirmed detection of pathogen from artificial infected seeds and natural Infected seeds using PCR assay. This assay gave results similar to a seed-wash dilution plating assay and proved more effective than classical methods. Results of survey on seed contamination by X. axonopodis pv. glycines from 36 cultivar seeds showed that the pathogen was detected from Pungsan-namulkong, Mallikong, Taekwangkong, Daemangkong, Ajukkarikong using PCR assay. Therefore, The PCR assay provides a sensitive, rapid tool for the specific detection of X. axonopodis pv. glycines in soybean seeds.

• Journal of Agricultural Extension & Community Development
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• v.8 no.1
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• pp.25-40
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• 2001

The purposes of the study were to identify satisfaction and achievement of learning by female learner's characteristics, and to suggest measures to improve quality of education in internet education programs. In order to determine the educational effectiveness associated with the characteristics of learners, this study attempted to employ two way approaches by observing the degree of achievement for learning, which represents an instructor-oriented approach, and the degree of satisfaction for education, which represents a learner-oriented approach to enhance the quality of internet education for female learners. As an approach to evaluate the educational effectiveness, the degree of achievement in learning(Tyler's classical approach), and the degree of satisfaction for education (Scriven's consumer-oriented evaluation model) were utilized. A survey form was developed by the researcher after reviewing the various tools originated from Boshier, Cross, Gagne and Choi, and distributed to a panel of judges that examined the content validity of the instrument. The sample for the study consisted of 160 female learners from three universities in Seoul and capital area, and the survey form was used to collect data for this study. The SPSS WIN program was used in analyzing the data and a series of statistical tests were conducted including frequency, percentile, t-test, ANOVA, correlation, multiple regression, and factor analysis. The statistical significance level was 0.05. The following conclusion were drawn from this study of female internet education. First, it was evident that female internet learners tend to utilize information from internet, and this can be interpreted as participants' positive attitude, and voluntary participation. Second, educational facilities and services should be improved in the future, because the level of satisfaction was low in these areas compared to curriculum and educational methodology. Third, the participating factors influenced by the level of satisfaction for education of learner characteristics were the 'formation of inter-personal relationship and willingness to change' and the 'needs for education on internet', thus appeared that both social and educational needs influenced the level of satisfaction for education. Fourth, the degree of achievement in learning was higher in the order of 1) attitude 2) function 3) knowledge, thus, attitude change was the most important in achievement of learning. Fifth, the individual background that influenced the level of achievement in learning were age and educational experience. As for the individual level of achievement for learning, the younger and more educated group were more satisfied.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.1
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• pp.62-72
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• 2010

PM OLED is used in many applications as one of the display for the next generation. The most essential problems are the power dissipation and the short life time in applying PM OLED into a commercial application. Many efforts are made in developing the panel and in improving the circuit for expanding the current market wider. The life time in PM OLED is expanded by lessening the power dissipation of the circuit for the magnitude of the driving current is lowered. It is possible to minimize the power dissipation from improving the driving technology. The classical technology, Row-to-Row driving, is that row is selected one by one while applying the column current input individually. The multi-line driving is a new technology which is to select multiple rows simultaneously while applying the column current as a whole. However, the solution of the multi-line driving is NP-complete problem. The efficiency is dependant on the sort of picture and the driving condition. This paper presents the new efficient multi-line driving which is that the multiple lines are selected by applying column current together after grouping the simultaneous driving group applying the gnew efficient muthe coi-line dr coefficient. Bengrouping the several rows which has the higher coi-line dr coefficient, the more efficient driving is realized to present the high quality image and to lessen the power dissipation and to stretch the life time in the PM OLED.

• Journal of the Earthquake Engineering Society of Korea
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• v.7 no.3
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• pp.69-77
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• 2003

This paper presents test results on eight reduced beam section(RBS) steel moment connections. The testing program addressed bolted versus welded web connection and panel zone(PZ) strength as key variables, Specimens with medium PZ strength were designed to promote energy dissipation from both PZ and RBS regions such that the requirement for expensive doublet plates could be reduced. Both strong and medium PZ specimens with a welded web connection were able to provide satisfactory connection rotation capacity for special moment-resisting frames. On the other hand, specimens with a bolted web connection performed poorly due to premature brittle fracture of the beam flange of the weld access hole. If fracture within the beam flange groove weld was avoided using quality welding, the fracture tended to move into the beam flange base metal of the weld access hole. Plausible explanation of a higher incidence of base metal fracture in bolted web specimens was presented. The measured strain data confirmed that the classical beam theory dose not provide reliable shear transfer prediction in the connection. The practice of providing web bolts uniformly along the beam depth was brought into question. Criteria for a balanced PZ strength improves the plastic rotation capacity while reduces the amount of beam distortion ore also proposed.

• Korean Journal of Mineralogy and Petrology
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• v.33 no.1
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• pp.19-28
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• 2020

Ranciéte is a hexagonal phyllomanganate mineral containing random Mn(IV) vacancies with hydrated Ca²⁺ cations charged balanced as interlayer cations. Its Mn²⁺ analogue is called takanelite, and ranciéite and takanelite are regarded as end-members of a solid solution series of (Ca²⁺,Mn²⁺)Mn₄O₉·nH₂O. Because the minerals are found as very small particles associated with other minerals, the crystal structures of the solid solution series have yet to be defined. In this research, we conducted classical molecular dynamics (MD) simulations of ranciéite and takanelite by varying the Mn²⁺/Ca²⁺ interlayer cation ratio to find relations between the interlayer cations and mineral structures. MD simulation results of chalcophanite group minerals are compared with experimental results to verify our method applied. Then, lattice parameters of ranciéite and takanelite models are presented along with detailed interlayer structures as to the distribution and coordination of cations and water molecules. This study shows the potentials of MD simulations in entangling complicated phyllomanganates structures.

• Safety and Health at Work
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• v.8 no.2
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• pp.198-205
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• 2017

Background: There are a million ragpickers in India who gather and trade recyclable municipal solid wastes materials for a living. The objective of this study was to examine whether their occupation adversely affects their immunity. Methods: Seventy-four women ragpickers (median age, 30 years) and 65 age-matched control housemaids were enrolled. Flow cytometry was used to measure leukocyte subsets, and leukocyte expressions of $Fc{\gamma}$ receptor I (CD64), $Fc{\gamma}RIII$ (CD16), complement receptor 1 (CD35) and CR3 (CD11b/CD18), and CD14. Serum total immunoglobulin-E was estimated with enzyme-linked immunosorbent assay. Results: Compared with the controls, ragpickers had significantly (p < 0.0001) higher levels of CD8-T-cytotoxic, CD16+CD56+natural killer, and CD4+CD45RO+memory T-cells, but depleted levels of CD19+B-cells. The percentage of CD4+T-helper-cells was lower than the control group (p < 0.0001), but their absolute number was relatively unchanged (p = 0.42) due to 11% higher lymphocyte counts in ragpickers. In ragpickers, the percentages of CD14+CD16+intermediate and CD14dim CD16+nonclassical monocyte subsets were elevated with a decline in CD14+CD16-classical monocytes. The expressions of CD64, CD16, CD35, and CD11b/CD18 on both monocytes and neutrophils, and CD14 on monocytes were significantly higher in ragpickers. In addition, ragpickers had 2.7-times more serum immunoglobulin-E than the controls (p < 0.0001). After controlling potential confounders, the profession of ragpicking was positively associated with the changes. Conclusion: Ragpicking is associated with alterations in both innate (neutrophils, monocytes, and natural killer cell numbers and expression of complement and $Fc{\gamma}$ receptors) and adaptive immunity (numbers of circulating B cells, helper, cytotoxic, and memory T cells).

• Science of Emotion and Sensibility
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• v.23 no.1
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• pp.29-40
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• 2020

Visually impaired people use tactile maps to get spatial information about their surrounding environment, find their way, and improve their independent mobility. However, classical tactile maps that make use of braille to describe the location within the map have several limitations, such as the lack of information due to constraints on space and limited feedback possibilities. This study describes the development of a new multi-modal interactive tactile map interface that addresses the challenges of tactile maps to improve the usability and independence of visually impaired people when using tactile maps. This interface adds touch gesture recognition to the surface of tactile maps and enables the users to verbally interact with a voice agent to receive feedback and information about navigation routes and points of interest. A low-cost prototype was developed to conduct usability tests that evaluated the interface through a survey and interview given to blind participants after using the prototype. The test results show that this interactive tactile map prototype provides improved usability for people over traditional tactile maps that use braille only. Participants reported that it was easier to find the starting point and points of interest they wished to navigate to with the prototype. Also, it improved self-reported independence and confidence compared with traditional tactile maps. Future work includes further development of the mobility solution based on the feedback received and an extensive quantitative study.

• Journal of Educational Research in Mathematics
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• v.27 no.1
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• pp.113-135
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• 2017

The purpose of the research was to develop an analysis framework for Korean mathematics textbooks from a critical mathematics education perspective. For this, we conducted a comprehensive literature review regarding critical theory, critical education, and critical mathematics education. Based on the literature review, we derived a preliminary framework for textbook analysis. To validate the preliminary framework delphi survey was carried out twice with 21 expert panelists in the field of mathematics education and multicultural education. The first delphi survey was conducted with open-ended questions to investigate diverse opinions regarding educational goals, contents, and teaching methods of critical mathematics education. The second delphi survey was conducted with Likert-type scale and it was analyzed using Mean, Contents Validity Ratio, Degree of Consensus. As the result of the whole research procedures, the final analysis framework was developed consisting of four categories: classical knowledge, community knowledge, communicative knowledge, and political knowledge. A development of the analysis framework from a critical mathematics education perspective could give a significant impact on the mathematics curriculum or mathematic teacher education in the Korea and a meaningful initial step for the effort of practicing critical mathematics education. It is expected that this study could not only incite consideration for the better mathematics education but also expand the prospect of research and practice in mathematics education. This study would provide a new paradigm of future mathematics education with which to teach and guide students to become members of world civil society with mathematical power and critical competency.

• The Journal of Internal Korean Medicine
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• v.19 no.1
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• pp.505-523
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• 1998

Aroma therpay in the western classical medicine is compared with the mechanistic paradigm of Newton physics which is the foundation of western modern medicine. It is the therapy whose basic principle is very similar to the basic concepts of oriental medicine lasting thousands of years. We know that parts of their therapies are explained by Yin-Yan and five elements in Oriental Medicine. In the modern western medicine, as preventive medicine is becoming more and more important and moreover, health promotion and self healing care is being emphasized, Maintenanayce which is the oriental preventive medicine, acts in concert with the concept of New Age of Science and provides a way for the Oriental Medicine of the future. The health promotion and self healing care is similar to the thought of Maintenanayce in Oriental Medicine. Oriental Medicine has provided the concept of preventive ehaling care for a long time and built the foundation of the department of Maintenanayce. Among these method of Preservation of Health, We could find that of using fragrance throuth the several references. By comparing the department of Maintenanayce in oriental preventive medicine with the books and papers about aroma therapy, used in the fields of naturalpathy medicine, alternative medicine, and complementary medicine in the west, and by analyzing them, centering around references, to see whether they are reasonable and corresponding, the following conclusions have been reached. 1. We could see that aroma therapy of oriental preventive medicine could be applied to the fields of health promotion and self healing cure, recently appearing in the world of health medicine, as a modern way of Preservation of Health(養生). 2. We could see that western traditional aroma therapy take important position in alternative medicine and complementary medicine, and it could be developed as the transitional field of medical cure for the interchange between western and eastern medicine. 3. We could see that aroma therapy is not totally unknown therapy and there is some points of similarity in the traditional references of oriental medicine. Also, it is considered that the aroma therapy has a possibility of coming the front as an general therapy among various therapies. 4. aroma therapy will be actively applied to many fields such as oriental psychiatry, internal medicine of the respiratory system, pediatrics, oriental nursing, oriental dermatologic beauty, and preventive medicine. Therefore, we have a view that the combination between aroma therapy and oriental medicine will be fairly valuable to study as the general and transtional middle step on which it will prepare the situation of oriental medicine's reimportation from the west and we establish a bridgehead to export the oriental medicine.