• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.1
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• pp.1-7
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• 2010

This study was carried out to investigate total polyphenol contents, total flavonoid contents and antioxidative activities of the methanol extracts from dried Citrus sunki and C. unshiu peels. The dried C. sunki peels displayed higher total polyphenol and flavonoid contents for older dried peels. In contrast, the dried C. unshiu peels displayed higher total polyphenol contents for more recently dried and displayed high total flavonoid contents by order of 2007, 2008 and 2006. DPPH radical scavenging activities of dried C. sunki peels were high by order of 2007 (0.25 mg/mL exception), 2006 and 2008, but C. unshiu peels could not confirm clear regularity. In particular, the dried C. sunki (2008, exception) and C. unshiu peels displayed very high activities of more than 86% in 1.0 mg/mL. Alkyl radical scavenging activities of dried C. sunki peels were higher for older dried peels (2007, 1.0 mg/mL exception), and that of dried C. unshiu peels were higher for more recently dried in below 0.5 mg/mL but were higher for older peels in 1.0 mg/mL. Hydrogen peroxide scavenging activities of dried C. sunki peels were higher for older peels (2007, 2.5 mg/mL exception), on the other hand, dried C. unshiu were higher for more recently dried. In particular, dried C. unshiu peels displayed high activities of more than 85% in 2.5 mg/mL. Hydroxyl radical scavenging activities of dried C. sunki and C. unshiu peels displayed low activities compared to other reactive oxygen species as the range of $49.9\pm2.2\sim63.5\pm0.9$% in 1.0 mg/mL.

• Journal of Life Science
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• v.24 no.7
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• pp.750-756
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• 2014

Citrus, yield of which is the highest among fruits produced in Korea, is extensively consumed for processed food items. The amount of by-products of citrus produced from the processing within a short period after the harvest is tremendous. These by-products are mostly dumped into land or neglected because of cost involved in processing them. The aim of the present study was to explore the usefulness of the by-products as a new material by examining the anti-inflammatory activity of fermented extracts of citrus peels. The peels of 'unshu' (Citrus unshiu) was fermented with Aspergillus niger and their extracts before or after fermentation were analyzed using HPLC. The analysis showed that neohesperidin level considerably increased and the two new compounds were synthesized after fermentation. The anti-inflammatory activity of the fermented extracts was examined on RAW 264.7 murine macrophage cells stimulated with lipopolysaccharide. Fermented unshu extracts significantly enhanced the decrease of nitric oxide (NO) production, iNOS and COX-2 expression, comparing with those of unfermented extracts. Also TNF-${\alpha}$ and IL-6 production, both of which are pro-inflammatory cytokine, were more inhibited in fermented extracts. These results showed that the fermentation and promotion of the function of the by-products of citrus peels will help find a new application.

• Korean journal of applied entomology
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• v.48 no.4
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• pp.535-539
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• 2009

This study was conducted to identify plant-parasitic nematodes and determine the distribution pattern of the nematode species in citrus fruit orchards in Korea from April to August, 2008. Plant-parasitic nematodes were found in 139 among 178 soil samples. Tylenchulus semipenetrans was collected from 85 out of 94 Citrus unshiu orchards examined (90.4%). C. junos orchards infested with T. semipenetrans were 60 out of 84 (71.4%). However, proportion of dominant species between the two was different. At domestic Citrus orchards, it proved that T. semipenetrans was the most important plant-parasitic nematode from this study. The overall frequency of the plant-parasitic nematodes was positively correlated with the cultivation years. More alkaline soils tended to harbor more T. semipenetrans. However the correlation coefficient between the two was very low.

• Korean Journal of Pharmacognosy
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• v.49 no.2
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• pp.113-123
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• 2018

The application of 112 samples of Citrus fruit peel, which is commonly cultivated in Korea as crude drug, has been limited due to the absence of criteria concerning chemical constituents and application of itself. In the context, this study is on the analysis of 11 flavonoid compounds. As a result, the flavonoid pattern could be classified into 5 groups. Furthermore, by utilizing this HPLC data, this study could provide a basis for fruit peel applications of not only Citrus unshiu, but also its cultivars, hybrids and some of Citrus plants which has been cultivated in Jeju Island for a long time. Moondan could be used for the same purposes as Chinese Huajuhong, and Poncirus trifoliata of Korean Jisil origin are effective enough, in terms of ingredients and function, compared with C. aurantium. Based on the content and composition of the PMF region in this study, C. unshiu and C. reticulata, as Tanaka System shows, should be considered as different hortcultural speicies. Also, Jikak, which is known as 'sour orange' until now, is confirmed as Chosen-daidai, C. tenuissima, that was reported by Tanaka in 1970, and this C. tenuissima could be used for the same purposes as sour orange.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.121.1-121
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• 2003

Dispersal of Xanthomonas axonopodis pv. citri, causing citrus bacterial canker disease on Unshiu orange was investigated at previously infested plots at Seogwipo in Jeju island of Korea. The bacterial pathogen overwintered in lesions started to multiply at tate May, and disease firstly observed one month after detection of phage from lesions. The disease gradually increased, however, it dispersed non-directionally to nearby plants from inoculum sources. Diseased plants were aggregated to form a cluster throughout the experiment. Population dynamics of phage on symtomless leaf surface and the disease severity were compared in the nursery, Increase of phage population on symptomless leaf surface preceded one month to that of the disease severity Population of phage increased constantly from late July to October, however, the disease severity decreased from late August to late October. It was assumed that the decrease of disease severity might be due to disease-induced defoliation.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.8
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• pp.1799-1804
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• 2012

Anti-Pseudomonas aeruginosa activities for ethanol extract of 34 medicinal plants widely used in the folk medicine were evaluated to screening of anti-Pseudomonas aeruginosa agents. The ethanol extracts of Gardenia jasminoides, Arctium lappa, Citrus unshiu, and Phellodendron amurense showed antimicrobial activities against P. aeruginosa ATCC 27853. The ethanol extracts of Gardenia jasminoides among these medicinal plants showed significant antimicrobial activity against P. aeruginosa. Therefore, we expect that these medicinal plants will be useful for nature antimicrobial agent against P. aeruginosa in future.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.1087-1090
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• 2009

This study was carried out to research antimicrobial agents from medicinal plants, Glycyrrhiza uralensis, Dryopteris crassirhizoma, Dictamnus albus, Paeonia lactiflora, Angelica dahurica, Spirodela polyrhiza, Cimicifuga heracleifolia, Bupleurum falcatum, Magnolia kobus, Artemisia princeps, Arctium lappa, Aster tataricus, Hovenia dulcis, Citrus unshiu, Asparagus cochinchinensis, Gardenia jasminoides, Smilax china, Hovenia dulcis, Prunus sargentii, Scutellaria baicalensis. The ethanol extracts of 20 medicinal plants were tested for the antimicrobial activity against Agrobacterium tumefaciens. The extracts of Glycyrrhiza uralensis, Dryopteris crassirhizoma, Cimicifuga heracleifolia, Bupleurum falcatum showed antimicrobial activities against Agrobacterium tumefaciens.

• Korean journal of applied entomology
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• v.47 no.4
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• pp.467-472
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• 2008

During the survey of plant parasitic nematode of Citrus orchard in Korea, Paratrichodorus minor (Colbran, 1956) Siddiqi is founded from the soil of Citrus unshiu Markovich for the first time in Korea. The body length of females is $428.34{\sim}506.22{\mu}m$ long: a = $12.08{\sim}22.79$, b = $4.02{\sim}5.02$, V(%) = $38.46{\sim}60.68$. The excretory pore is located near the base of esophagus. Additionally, the morphological characteristics on P. porosus (Allen, 1957) Siddiqi, 1974 recorded in 1976 on Korea is redescribed in detail.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.115-124
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• 2024

Citrus cultivation plays a pivotal role, making a significant contribution to global fruit production and dietary consumption. Accurate identification of viral pathogens is imperative for the effective management of plant viral disease in citrus crops. High-throughput sequencing serves as an alternative approach, enabling comprehensive pathogen identification on a large scale without requiring pre-existing information. In this study, we employed HTS to investigate viral pathogens infecting citrus in three different regions of South Korea: Jejudo (Jeju), Wando-gun (Wando), and Dangjin-si (Dangjin). The results unveiled diverse viruses and viroids that exhibited regional variations. Notably, alongside the identification of well-known citrus viruses such as satsuma dwarf virus, citrus tatter leaf virus, and citrus leaf blotch virus (CLBV), this study also uncovered several viruses and viroids previously unreported in Korean citrus. Phylogenetic analysis revealed that majority of identified viruses exhibited the closest affilations with isolates from China or Japan. However, CLBV and citrus viroid-I-LSS displayed diverse phylogenetic positions, reflecting their regional origins. This study advances our understanding of citrus virome diversity and regional dynamics through HTS, emphasizing its potential in unraveling intricate viral pathogens in agriculture. Consequently, it significantly contributes to disease management strategies, ensuring the resilience of the citrus industry.

• The Korea Journal of Herbology
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• v.30 no.5
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• pp.59-65
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• 2015

Objectives : Fermentation of herbs has been known to be helpful in improving the immune systems and protecting body against disease. The present study was conducted to evaluate anti-inflammatory effects of the fermentation extracts (FE) consisting of soybean, red ginseng andCitrus UnshiuPeel in lipopolysaccharide (LPS)-activated Raw264.7 cells.Methods : FE were prepared by the fermentation withBacillus Subtilisand then by extraction with ethanol (95%; prepared by the fermentation process). Cell viability was measured by MTT assay. Nitric oxide (NO) production was measured in culture media by Griess assay. The expression of nuclear factor (NF)-κB and inhibitory kappa B alpha (IκBα) was determined by Western blot.Results : LPS-induced production of NO and PGE₂was dose-dependently decreased by the treatment of FE in Raw264.7 cells. These suppressive effects of FE on NO and PGE₂production were related to the inhibition of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. FE inhibited LPS-induced production of pro-inflammatory cytokines, TNF-α, IL-6, and IL-1βin a dose-dependent manner. Furthermore, FE inhibited the NF-κB signaling pathway through the prevention of LPS-induced degradation of IκBαin cytosol and the nuclear translocation of NF-κB.Conclusions : These findings suggest that FE could have anti-inflammatory effects on LPS-induced inflammatory responses in macrophages.