• The Korean Journal of Pesticide Science
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• v.20 no.4
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• pp.355-360
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• 2016

The insecticidal activities of the essential oils of Artemisia vulgaris, Achillea millefolium, Anethum graveolens, Cimicifuga heracleifolia, Citrus paradisi, Chrysanthemum indicum, Eucalyptus dives, Neroli bigard, Nigella sativa, and Ruta graveolens against stored-grain insects, Plodia interpunctella and Tribolium castaneum larvae and adults were evaluated using fumigant and contact bioassays. Based on the $LD_{50}$ values in fumigant bioassay, A. millefolium ($0.12{\mu}g/cm^3$) and A. graveolens ($93.14{\mu}g\;cm^{-3}$) oil were most effective against P. interpunctella and T. castaneum adults. In addition, N. bigard ($139.11{\mu}g\;cm^{-3}$) and A. graveolens ($52.87{\mu}g\;cm^{-3}$) oil exhibit strong insecticidal activities against P. interpunctella and Tribolium castaneum larvae in contact bioassay. These results indicate that A. millefolium, A. graveolens, N. bigard, and A. graveolens oils could be effective natural acaricides for managing stored-grain insects, P. interpunctella and T. castaneum.

• Korean Journal of Medicinal Crop Science
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• v.21 no.6
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• pp.493-497
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• 2013

This study was designed to analyze the chemical composition of essential oil in 'Shiranuhi' immature fruit and to test their biological activities. 'Shiranuhi' immature essential oils (SIEO) were obtained by steam distillation from fruits collected from Jeju Island and were analyzed using gas chromatograph (GC)-flame ionization detectors (FID) and GC-MS. Fourteen components were identified in the essential oil. Limonene (75.21%) and terpineol (8.68%) were the major components in SIEO. Since acne vulgaris is the combined result of a bacterial infection and the inflammatory response to that infection, we examined whether SIEO possessed antibacterial against skin pathogens. As a result, SIEO showed excellent antibacterial activities against drug-susceptible and -resistant Propionibacterium acnes and Staphylococcus epidermidis, which are acne-causing bacteria. In this study, SIEO was examined on DPPH radical scavenging activities, which showed moderate antioxidant activity ($SC_{50}$, $15.36{\mu}L/mL$). In order to determined whether SIEO can be safely applied to human skin, the cytotoxicity effects of SIEO were determined by colorimetric MTT assays in normal human fibroblasts and keratinocyte HaCaT cells. They exhibited low cytotoxicity at $0.5{\mu}L/mL$ in both celllines. Based on these results, we suggest the possibility that essential oil of 'Shiranuhi' maybe considered as an antibacterial and antioxidant agent.

• Journal of Microbiology and Biotechnology
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• v.30 no.6
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• pp.878-884
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• 2020

Penicillium digitatum and P. italicum are the two important postharvest pathogens in citrus, causing about 90% of the total loss of citrus fruit during storage and transportation. Natural fungicides such as essential oils have been widely used instead of chemical fungicides for preventing and controlling postharvest diseases. In this research, p-anisaldehyde exhibited a strong inhibitory effect on P. digitatum and P. italicum, with the minimum inhibitory concentration and minimum fungicidal concentration values of both being 2.00 μl/ml. Additionally, p-anisaldehyde visibly inhibited both the green mold and blue mold development of citrus fruits inoculated with P. digitatum and P. italicum. The mycelia morphologies of these pathogens were greatly altered, and the membrane permeability and cell wall integrity of mycelia were severely disrupted under p-anisaldehyde treatment. These results suggest that the antifungal activity of p-anisaldehyde against P. digitatum and P. italicum can be attributed to the disruption of the cell wall integrity.

• Journal of Mycology and Infection
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• v.23 no.4
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• pp.91-98
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• 2018

Background: Malassezia, a lipophilic yeast, is a causative agent for dandruff and seborrheic dermatitis. Many biological agents have been studied for anti-Malassezia effect but further studies are needed for their clinical application. Objective: The study was conducted to evaluate the inhibitory effect of different natural essential oils and a fruit extract on Malassezia species in an in vitro study and a clinical trial. Methods: The antifungal effects of natural essential oils and a fruit extract on Malassezia species (M. furfur and M. sympodialis) were evaluated by measuring the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) and using the disc diffusion method. Natural essential oils of citron seed, lavender, and rosemary and citrus junos fruit extract were used for the in vitro study. The clinical trial was conducted with a shampoo containing four ingredients. A total of 22 subjects used the shampoo every day for 4 weeks and were evaluated using clinical photography, trichoscopy, and sebumeter at baseline, 2 weeks, and 4 weeks after treatment. Results: Antifungal activity of agents was relatively lower in lavender and rosemary essential oils at MIC and MFC. Disc diffusion method revealed same results. In the clinical trial, the amount of sebum decreased statistically significantly and erythema, dandruff, and lesion extent also improved. Conclusion: The natural essential oils and fruit extract are effective for suppressing Malassezia activity, therefore these might be used as an alternative for treatment of dandruff and seborrheic dermatitis.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2001.06a
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• pp.175-178
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• 2001

최근 호주 및 미국의 오렌지나 사과 등의 과수재배에서는 천적과 함께 석유 유래의 petroleum spray oils (PSOs)을 이용한 병해충종합관리(IPM) 체계가 적극적으로 도입되고 있다. PSO 제제는 천적, 인축 및 환경잔류 독성이 낮으며 해충에 대해서는 호흡곤란 및 기피작용에 의한 방제효과로 약제저항성을 유발시키지 않는다는 장점이 있으나 식물독성 유발의 위험성 때문에 이용의 확대가 제한되었다.(중략)

• Korean Journal of Food Science and Technology
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• v.49 no.3
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• pp.242-251
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• 2017

The purpose of this study was to investigate the anti-proliferative effect of methanolic extracts from Citrus junos (yuja) seeds and yuja seed oils against HT-29 human colon cancer cells and to identify the key compounds responsible for this effect. Extracts from yuja seeds, yuja seed oil prepared using hexane, and cold-pressed yuja seed oil were prepared using 60% methanol (ES, EHO, and ECO, respectively). The key compounds in the extracts were determined using HPLC-MS. Among the extracts, EHO and ECO inhibited proliferation of HT-29 cells. EHO and ECO were fractionated using preparative LC and the bioactive compounds were determined. Five of the fractions showed a significant anti-proliferative effect and the main compounds in the fractions were isopimpinellin, bergapten, and ichangensin. These compounds showed anti-proliferative effects on HT-29 cells when treated individually, and ichangensin showed the highest anti-proliferative activity. These results suggest that these compounds may be responsible for the anti-cancer effect of EHO and ECO.

• Korean Journal of Medicinal Crop Science
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• v.27 no.6
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• pp.427-435
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• 2019

Background: A growing interest in health has increased the need for the development of potent antioxidant materials known to play a role in various physiological activities. Currently research and development of non-toxic natural antioxidants with high activity is ongoing. Methods and Results: In this study, we measured 2,2'-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability of 10 plant essential oils, selecting samples of Dendranthema indicum, Dendranthema zawadskii, and Citrus sunki essential oils. The samples were analyzed using liquid chromatography (LC) and the radical scavenging activity on LC-based systems with the same conditions. In the LC-mass spectroscopy (MS)/MS analysis of the active compound peak, 2-methoxy-4-vinylphenol with a molecular weight of 150.1 g/mol was identified in C. sunki essential oils. Eugenol or isoeugenol with a molecular weight of 164.1 g/mol as identified in D. indicum and D. zawadskii essential oils as radical scavenging active compounds. Conclusions: In the LC-based measurement system, the active ingredient can be identified by simultaneously conducting profile analysis and the radical scavenging activity of essential oil samples. In addition, LC-MS/MS analysis of the active compound peaks can be performed under the same separation conditions to obtain data that can identify the active compounds in the sample.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.1
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• pp.23-30
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• 2021

This study is an experiment to evaluate the anti-atopy efficacy of five kinds of natural plant essential oils; Artemisia annua L. (AA), Citrus junos Sieb. ex TANAKA (CJ), Chrysanthemum boreale Makino (CB), Pinus koraiensis (PK), and Pinus densiflora for. erecta (PD). Through Agar diffusion test, five species of native plant essential oils were treated in a total of four strains, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. In order to invest the anti-inflammatory effect, five kinds of natural plant essential oils were treated in HaCaT cells-induced by TNF-α and IFN-γ (TI). AA, CJ, CB, PK and PD showed antibacterial effects on Candida albicans at a concentration of 10 mg/mL. We also found that the thymus and activation-regulated chemokine (TARC) expression was suppressed in 0.1 ㎍/mL of PK, 1 ㎍/mL of AA, CB, and PK. macrophage-derived chemokine (MDC) expression was suppressed in 1 ㎍/mL of AA and PK. IL-6 expression was suppressed in 0.1, 1 ㎍/mL of AA, PK in HaCaT cells. Hence it suggests that AA, CB, and PK have the anti-inflammatory effects, and it could contribute to atopic dermatitis relief by reducing the infiltration of immune cells to inflamed area.

• Journal of Environmental Science International
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• v.29 no.10
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• pp.997-1002
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• 2020

In this study, we investigated the effects of the eco-friendly chemical bio-sulfur, on the citrus melanose-causing pathogen, Diaporthe citri, and on the pest, Panonychus citri. In an open field experiment with a plot-scale application of the chemicals: Mancozeb, lime sulfur, lime sulfur + machine oil, and bio-sulfur, the control group showed 70.6% disease severity compared with 10.3% for the Mancozeb-treated group. Among the eco-friendly treatments, disease severity was the lowest for the group treated with lime sulfur + machine oil (32.2%) and was 53.9%, 58.8%, and 58.1% following treatment with lime sulfur, and bio-sulfur diluted 500 and 1000 times, respectively. The proportion of diseased fruit showed similar results, suggesting that bio-sulfur is an effective alternative to lime sulfur. Three days after treatment acaricidal effects on P. citri showed a 197.6% control survival rate whereas the machine oil, and bio-sulfur diluted 500 and 1000 times treatments showed rates of 2.9%, 5.8%, and 9.0%, respectively. After three days, the control value for bio-sulfur diluted 1000 times was 73.2% compared with the values for the machine oil (96.4%) and bio-sulfur diluted 500 times (94.6%) treatments. Therefore, we suggest that additional research is needed on the combined application of bio-sulfur and oils to enhance the additive control effect on citrus melanose and Panonychus citri.

• Microbiology and Biotechnology Letters
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• v.52 no.1
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• pp.24-36
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• 2024

This study determines the combinatorial effect of enterocin MSW5 and five essential oils (EOs- Thymus vulgaris, Cymbopogon martini, Origanum vulgare, Cinnamomum zeylanicum, and Cymbopogon citrus) against Staphylococcus aureus, Listeria monocytogenes, and Salmonella Typhimurium. The Minimum Inhibitory Concentration of each antimicrobial agent was determined. The MIC of enterocin MSW5 against test pathogens was in the following order: S. aureus (0.362 ± 0.01), S. Typhimurium (0.362 ± 0.05 mg/ml), L. monocytogenes (0.725 ± 0.08 mg/ml). Among all EOs, maximum activity was observed in the case of C. zeylanicum against S. aureus (78.12 ± 0.04 ppm), S. Typhimurium (78.12 ± 0.08 ppm), and L. monocytogenes (39.00 ± 0.05 ppm). Further, the checkerboard assay was used to determine the synergistic effect between antimicrobial agents and enterocin MSW5 in combination with C. zeylanicum has shown significant synergism with the Fraction Inhibitory Concentration index (0.372) against test pathogens. Additionally, individual EOs and enterocin MSW5 have shown anti-biofilm activity, whereas their combined use has shown more significant antibiofilm activity. The maximum anti-biofilm activity was observed with the combination of enterocin MSW5 and O. vulgares against S. aureus (92.86 ± 0.06%) and S. Typhimurium (73.63 ± 0.23%) and a combination of enterocin MSW5 and C. citrus against L. monocytogenes (87.84 ± 0.15%). Therefore, combinations of antimicrobial compounds can control the growth of foodborne pathogens better than the individual agent.