Hydrophobic core variant of ubiquitin appeared to have partially folded structure at pH around 2. The intrinsic tryptophan fluorescence emission maximum of this ubiquitin variant at pH 2 showed slight blue shift compare to that of unfolded state, suggesting that some residual tertiary structures remain in this solvent condition. At the same solvent condition, this ubiquitin variant binds with hydrophobic dye, 8-anilinonaphthalene-1-sulfonic acid(AMS), which is known to bind to exposed hydrophobic surface. Furthermore, far-UV circular dichroic spectrum of this ubiquitin variant in the diminished pH was remarkably different from the far-UV CD spectrum of the native state or unfolded state. Based on the molar ellipticity at 220 nm, this ubiquitin variant at pH 2 appeared to have significant amount of secondary structures. All these observations suggest that this ubiquitin variant in the diminished solvent pH has loosely folded hydrophobic core with some secondary structures, which are key features of molten globule conformation. Since molten globule has long been considered as a protein folding intermediate, it is considered that this hydrophobic core variant ubiquitin will serve as a valuable model to study protein folding process.
Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the leading infectious diseases to humans. It is urgent to discover novel drug targets for the development of antitubercular agents. The 2-C-methyl-Derythritol-4-phosphate (MEP) pathway for isoprenoid biosynthesis has been considered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammals. MEP cytidyltransferase (IspD), the third-step enzyme of the pathway, catalyzes MEP and CTP to form 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) and PPi. In the work, ispD gene from M. tuberculosis H37Rv (MtIspD) was cloned and expressed. With N-terminal fusion of a histidine-tagged sequence, MtIspD could be purified to homogeneity by one-step nickel affinity chromatography. MtIspD exists as a homodimer with an apparent molecular mass of 52 kDa. Enzyme property analysis revealed that MtIspD has high specificity for pyrimidine bases and narrow divalent cation requirements, with maximal activity found in the presence of CTP and $Mg^{2+}$. The turnover number of MtIspD is $3.4 s^{-1}$. The Km for MEP and CTP are 43 and $92{\mu}M$, respectively. Furthermore, MtIspD shows thermal instable above $50^{\circ}C$. Circular dichroism spectra revealed that the alteration of tertiary conformation is closely related with sharp loss of enzyme activity at higher temperature. This study is expected to help better understand the features of IspD and provide useful information for the development of novel antibiotics to treat M. tuberculosis.
Journal of the Computational Structural Engineering Institute of Korea
/
v.29
no.1
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pp.9-18
/
2016
The slender structures such as high rise building or marine riser are highly susceptible to dynamic force exerted by fluid-structure interactions among which vortex-induced vibration(VIV) is the main cause of dynamic unstability of the structural system. If VIV occurs in natural frequency regime of the structure, fatigue failure likely happens by so-called lock-in phenomenon. This study presents the numerical analysis of dynamic behavior of both structure and fluid in the lock-in regimes and investigates the subjacent phenomena to hold the resonance frequency in spite of the change of flow condition. Unsteady and laminar flow was considered for a two-dimensional circular cylinder which was assumed to move freely in 1 degree of freedom in the direction orthogonal to the uniform inflow. Fluid-structure interaction was implemented by solving both unsteady flow and dynamic motion of the structure sequentially in each time step where the fluid domain was remeshed considering the movement of the body. The results show reasonable agreements with previous studies and reveal characteristic features of the lock-in phenomena. Not only the lift force but also drag force are drastically increasing during the lock-in regime, the vertical displacement of the cylinder reaches up to 20% of the diameter of the cylinder. The correlation analysis between lift and vertical displacement clearly show the dramatic change of the phase difference from in-phase to out-of-phase when the cylinder experiences lock-in. From the results, it can be postulated that the change of phase difference and flow condition is responsible for the resonating behavior of the structure during lock-in.
Using light microscopy, the digestive tract of the parrot fish, Oplegnathus fasciatus was studied histologically. The tract consists of esophagus, stomach, intestine and fifty or sixty pyloric caecae. Each pyloric caecum is a blind sac of banana shape, and is originated from pyloric end of the stomach. The relative length of gut (RLG), that is length of digestive tract to standard length, is 1.78 (n=30). Esophagus has an undeveloped submucosa and a well developed muscularis mucosae. Its mucosa displays primary and secondary folds lined with a cuboidal or columnar epithelium and numerous acidic mucous secretory cells. The stomach has muscularis, which consists of longitudinal and circular muscular layers; its submucosa and muscularis mucosae are well developed. Only primary folds are present in the stomach. Microvilli are present in the epithelial layer. The stomach has a well-developed gastric gland and relatively more secretory granules. Mucosal epithelium of the pyloric caeca is composed of neutral mucous secretory cells and columnar epithelium with developed microvilli in the apical portion. Using the morphological and histological features, the intestine may be divided into anterior, mid and posterior parts. It has an advanced striated border and abundant acidic mucous secretory cells and some neutral mucous secretory cells. The mid intestine has more abundant acidic mucous secretory cells than the anterior and posterior parts. A thick longitudinal muscle is formed in the intestine and its thickness is progressively increased towards the posterior end.
Dan(緞) is satin damask. It was a newly popularized fabrics in the Joseon Period and is one of main traditional fabrics at present. The purpose of this study is to suggest a theoretical basis to name Dan(緞) remains properly by comparing and analyzing the name, pattern, usage, feature, length and width, value of Dan(緞) recorded on documents made at the end of the Joseon Period. The results of this study are as follows. 1. The kinds of Dan(緞) recorded on documents made at the end of the Joseon Period are various. Among fabrics named Dan(緞), were not only silk fabrics but also cotton fabrics, woolen fabrics, mixture fabrics with silk and cotton, and mixture fabrics with cotton and wool. 2. Some newly revealed patterns in the case of Dan(緞) are as follows. The shape of Byeolmun(別紋) is assumed to have originated from that of character symbolizing the Royal Family and developed into a variety of circular ones of dragon, phoenix, flower, or character. O-ho-ro-mun(五葫蘆紋) was five-gourd-shaped patterns that form a circle, turning their narrow mouth to the center, and vines between each two gourds. 3. When considering the usages of Dan(緞), Dan(緞) was used for various detailed usages according to their kinds. But it was't mostly used for underwear. 4. Features have been examined are Godan(庫緞), Mobondan(模本緞), Handan(漢緞), Daedan(大緞), Yangdan(洋緞), Waedan(倭緞), and Geumdan(錦緞). 5. The length and the width of 1 Pil(疋) silk fabrics are various. It is normal for the width being different, but for 1 Pil(疋)'s length being different is very unusual. The width of Dan (緞) was about 47-76.20cm, it was wider than Ju(紬), Cho, Gyeon(絹), Sa(紗), Ra(羅) and Reung(綾). 6. As for the value of each fabric per $10,000cm^2$, Dan(緞) was the most valuable silks at that time. Among them the most valuable Dan(緞) was Udan(羽緞).
Journal of the Korea Fashion and Costume Design Association
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v.4
no.2
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pp.139-161
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2002
This study investigates the characteristics of Nordic sweaters works from a historical perspective. Specifically, this study deals with the following research topics: 1) development of Nordic sweaters, 2) the characteristics of Nordic sweaters industry according to the change of times, 3) the comparison of local knitting patterns, 4) the symbolic meaning of the designs in the Nordic sweaters and patterns. The results of the study are summarized as follows. 1. Knitted work developed mostly in Northern Europe, a cold area, and the barren, mountainous coastal areas where people frequently used woolen materials for clothes. It was also developed in Scandinavian regions which lead the fashion in modern days. Scandinavian knitting techniques have been diffused into the east coast of England and Northern Europe by Vikings. 2. Scandinavian countries are distinguished from other countries by their conservative but creative cultural tradition. Their knitting patterns are characterized by small geometric figures such as dots, triangles, squares, rhombuses, and crosses used often with stars and roses. Scandinavian knitting is also salient for its vertical stripes and simple motifs repeating at short intervals. 1) Norway ; Simple and geometric Norwegian patterns are classified into three groups of motifs: (a) the motifs of cross, diamond, X, and swastika (equation omitted). (b) the motifs of human figures, animals and birds, (c) floral motifs (especially eight-petal roses). Their use of color is also simple, and is limited to more than two colors. (2) Sweden ; Swedish patterns are colorful and geometric. They are characterized by features such as brocade, complex embroidery, and contrast of red and black colors. They also show Guernsey patterns. Initials and production years were knitted in sweaters which have different patterns in their trunks and sleeves. 3) Denmark ; The Danish pattern is the purl stitch knitted against the stockinette stitch. The technique is used to copy woven damask motifs. The patterns are seen most clearly when they are knit with smooth yarn. The Faeroe sweaters are the representative work of Danish knitting. Faeroe knitting, incorporates stranded pattern and is knit in the round, either with circular needles. 4) Finland ; Finnish patterns are similar to Norwegian patterns. Finnish knitted work show very colorful, variety and free-flowing geometric patterns. 5) Iceland ; Icelandic knitting shows original ribbon pattern. Lope sweater is the representative work. 3. The traditional knitting patterns not only carried symbolic meanings but also served as means of communication. First of all, patterns had incantatory meanings. Patterns were symbolic of one's social standing, too. The colors, motifs and their arrangements were very important features symbolizing one's social position or family line. People often communicated by certain pieces of knitted work or patterns. In short, the knitted work in the Nordic sweaters served the function of admiring the beauty of nature and symbolizing various meanings. The unique designs and colors of the knitted work reflected the characteristics of the culture those works belonged to. This study also turns our attention to the issue of how the traditional colors and designs of the knitted work can contribute to the development of modern designs, and by doing so, if makes us realize the importance of knitted works in modern society.
Kim, Chan-Jong;Park, In-Sun;An, Hui-Soo;Oh, Phil-Seok;Kim, Dong-Young;Park, Young-Shin
Journal of the Korean earth science society
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v.26
no.8
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pp.751-758
/
2005
The purpose of this study was to develop an inquiry analysis framework based on the features of earth science inquiry methodology and to analyze inquiry activities in the 8th grade 'Earth History and Diastrophism' unit by using this framework. The framework classified earth science methods as logical inference, hermeneutic, and historical methods, each of which was subdivided in consideration of its subordinate methods and characteristics. The analysis revealed that the logical inference method reflected in the unit as the 'abductive method' (70%) was used more frequently than the 'inductive' (23%) and 'deductive' (22%) methods. The hermeneutic method was found in terms of the 'forestructures of understanding' (92%), 'circular reasoning' (9%). and 'historical nature of human understanding' (17%). The historical method also used as the 'constructing proper taxonomy' (53%), 'adhering to the modem principle of uniformitarianism' (47%), and 'relic interpretation' (41%) were identified with ratios more fester than those for the 'place substituting for time in stage theorizing' (3%) and 'evaluating independent lines of inquiry for convergence' (3%).
Comparative studies on mathematical modeling cognition feature were carried out between 15 excellent high school third-grade science students (excellent students for short) and 15 normal ones (normal students for short) in China by utilizing protocol analysis and expert-novice comparison methods and our conclusions have been drawn as below. 1. In the style, span and method of mathematical modeling problem representation, both excellent and normal students adopted symbolic and methodological representation style. However, excellent students use mechanical representation style more often. Excellent students tend to utilize multiple-representation while normal students tend to utilize simplicity representation. Excellent students incline to make use of circular representation while normal students incline to make use of one-way representation. 2. In mathematical modeling strategy use, excellent students tend to tend to use equilibrium assumption strategy while normal students tend to use accurate assumption strategy. Excellent students tend to use sample analog construction strategy while normal students tend to use real-time generation construction strategy. Excellent students tend to use immediate self-monitoring strategy while normal students tend to use review-monitoring strategy. Excellent students tend to use theoretical deduction and intuitive judgment testing strategy while normal students tend to use data testing strategy. Excellent students tend to use assumption adjustment and modeling adjustment strategy while normal students tend to use model solving adjustment strategy. 3. In the thinking, result and efficiency of mathematical modeling, excellent students give brief oral presentations of mathematical modeling, express themselves more logically, analyze problems deeply and thoroughly, have multiple, quick and flexible thinking and the utilization of mathematical modeling method is shown by inspiring inquiry, more correct results and high thinking efficiency while normal students give complicated protocol material, express themselves illogically, analyze problems superficially and obscurely, have simple, slow and rigid thinking and the utilization of mathematical modeling method is shown by blind inquiry, more fixed and inaccurate thinking and low thinking efficiency.
Journal of Korean Society for Geospatial Information Science
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v.21
no.4
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pp.101-107
/
2013
Generally the commercial high-resolution images have their coordinates, but the locations are locally different according to the pose of sensors at the acquisition time and relief displacement of terrain. Therefore, a process of image co-registration has to be applied to use the multi-temporal images together. However, co-registration is interrupted especially when images include the cloud-covered regions because of the difficulties of extracting matching points and lots of false-matched points. This paper proposes an automatic co-registration method for the cloud-covered high-resolution images. A scale-invariant feature transform (SIFT), which is one of the representative feature-based matching method, is used, and only features of the target (cloud-covered) images within a circular buffer from each feature of reference image are used for the candidate of the matching process. Study sites composed of multi-temporal KOMPSAT-2 images including cloud-covered regions were employed to apply the proposed algorithm. The result showed that the proposed method presented a higher correct-match rate than original SIFT method and acceptable registration accuracies in all sites.
Prabu, R.;Vijayaraj, A.;Suresh, R.;Jagadish, L.;Kaviyarasan, V.;Narayanan, V.
Bulletin of the Korean Chemical Society
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v.32
no.5
/
pp.1669-1678
/
2011
Six new binuclear copper(II) complexes have been prepared by template condensation of the dialdehydes 1,8-[bis(3-formyl-2-hydroxy-5-methyl)benzyl]-l,4,8,11-tetraazacyclotetradecane (PC-a) and 1,8-[bis(3-formyl-2-hydroxy-5-bromo)benzyl]-l,4,8,11-tetraazacyclotetradecane (PC-b) with appropriate aliphatic diamines, and copper(II) perchlorate. The structural features of the complexes have been confirmed by elemental analysis, IR, UV-vis and mass spectra etc. The electrochemical behavior of all the copper(II) complexes show two irreversible one electron reduction process. The room temperature magnetic moment studies depict the presence of an antiferromagnetic interaction in the binuclear complexes. The catechol oxidation and hydrolysis of 4-nitrophenylphosphate were carried out by using the complexes as catalyst. The antimicrobial screening data show good results. The binding of the complexes to calf thymus DNA (CT DNA) has been investigated with absorption and emission spectroscopy. The complex [$Cu_2L^{1a}$] displays significant cleavage property of circular plasmid pBR322 DNA in to linear form. Spectral, electrochemical, magnetic and catalytic studies support the distortion of the copper ion geometry that arises as the macrocyclic ring size increases.
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