• Journal of Gastric Cancer
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• 제20권3호
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• pp.300-312
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• 2020

Purpose: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. Materials and Methods: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. Results: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_ circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. Conclusions: Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.

• 미생물학회지
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• 제54권2호
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• pp.167-168
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• 2018

Staphylococcus xylosus는 일반적으로 포유동물의 피부에 존재하며 식품가공설비와 의료기기 등에서도 발견이 보고되었다. 본 연구에서는 강력한 생물막 생성 특성을 가지는 Staphylococcus xylosus S170의 전체 유전체 서열을 분석하여 보고한다. 이 유전체는 2,910,005 bp 크기, 2,674개의 단백질 코딩 서열과 22개의 rRNA, 57개의 tRNA유전자를 포함한다. 본 연구에서 제공하는 유전체 정보는 생물막 관련 유전자 분석으로 생물막 형성 기작을 좀 더 잘 이해하는데 도움이 될 수 있다.

• Journal of Species Research
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• 제13권2호
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• pp.127-130
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• 2024

The first complete mitogenome sequence of the Red-tongue Pit Viper (Gloydius ussuriensis) from Korea was characterized using next-generation sequencing. The mitogenome is a circular molecule (17,209 bp) with a typical vertebrate mitogenome arrangement, which consists of 2 ribosomal RNA genes (rRNA), 22 transfer RNA genes (tRNA), two non-coding regions (D-loop), and 13 protein-coding genes (PCGs). The base composition of the mitogenome is 32.7% of A, 27.5% of C, 13.9% of G, and 25.9% of T, with a slight AT bias(58.6%). This phylogenetic analysis infers that G. ussuriensis is in the same group as the Chinese G. ussuriensis (Accession No. KP262412) and is closely related to G. blomhoffi and other species of the genus Gloydius. In our study, the complete mitogenome sequence of Korean G. ussuriensis was characterized and we provided basic genetic information on this species.

• 대한의생명과학회지
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• 제10권2호
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• pp.65-73
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• 2004

I report that single-stranded antisense as a part of large circular (LC-) genomic DNA of recombinant M13 phage exhibits enhanced stability, sequence specific antisense activity, and no need for target site search. A cDNA fragment (708 bp) of rat TNF-$\alpha$ was inserted into a phagemid vector, and TNF-$\alpha$ antisense molecules (TNF$\alpha$-LCAS) were produced as single-stranded circular DNA. When introduced into a rat monocyte/macrophage cell line, WRT7/P2, TNF$\alpha$-LCAS was able to ablate LPS-induced TNF-$\alpha$ mRNA to completion. The antisense effect of TNF$\alpha$-LCAS was shown to be sequence-specific because expressions of three control genes ($\beta$-actin, GAPDH and IL-1$\beta$) were not significantly altered by the antisense treatment. Further, TNF$\alpha$-LCAS was found to be highly efficacious as only 0.1 $\mu$g (0.24 nM) of TNF$\alpha$-LCAS was sufficient to block TNF-$\alpha$ expression in 1$\times10^5$ WRT7/P2 cells. I have also observed specific antisense activity in reduction of NF-$\kappa$B gene expression. The results suggest that an antisense sequence as a part of single-stranded circular genomic DNA has a specific antisense activity.

• 한국자기공명학회논문지
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• 제9권2호
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• pp.110-121
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• 2005

E.coli methionyl tRNA synthetase consist of 676 amino acids and plays a key role in initiation of protein synthesis. The native form of this enzyme is a homodimer, but the monomeric enzyme truncated approximately C-terminal 120 amino acids retains the full enzymatic activities. X-ray crystal structure of the active monomeric enzyme shows that it has two domains. The N-terminal domain is thought to be a binding site for acceptor stem of tRNA, ATP, and methionine. The C-terminal domain is mainly a-helical and makes an interaction with the anticodon of $tRNA^{Met}$. Especially it is suggested that the region of helix-loop-helix including the tryptophan residue at the position 461 may be the essential for the interaction with anticodon of $tRNA^{Met}$. In this work the structure and function of E. coli methionyl-tRNA synthetase was studied by spectroscopic method (NMR, CD, Fluorescence). The importance of tryptophan residue at the position 461 was investigated by fluorescence spectroscopy. Tryptophan 461 is expected to be an essential site for the interaction between E. coli methionyl-tRNA synthetase and E. coli $tRNA^{Met}$. Proton and heteonuclear 2-dimensional NMR spectroscopy were also used to elucidate the protein-tRNA interaction.

• 미생물학회지
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• 제55권2호
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• pp.171-173
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• 2019

Paenibacillaceae계의 Cohnella 속은 다양한 환경 속에서 서식한다. 여기에 우리는 한국 한라산 꼭대기의 구상나무(Abies koreana) 근권 토양으로부터 분리된 Cohnella sp. HS21의 전체 게놈 서열을 보고한다. 균주 HS21은 원형 염색체 7,059,027 bp와 44.8%의 GC 함량을 가지고 있다. 5,939개의 단백질 코딩 유전자와 78개의 트랜스퍼 (tRNA) 유전자, 27개의 리보솜 RNA (rRNA) 유전자, 4개의 비 코딩 RNA 유전자(ncRNA), 90개의 위 유전자가 존재했다. 박테리아는 항생제 관련 유전자 클러스터와 식물 세포벽 분해 효소를 코딩하는 유전자를 포함하고 있다.

• 한국자기공명학회논문지
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• 제13권2호
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• pp.126-134
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• 2009

UBLCP1 is composed of Ubiquitin Like domain and RNA Polymerase II Phosphatase I domain. Phosphatase domain (25.9KDa) has been cloned into the E.coli using pET32a vector with TEV protease cleavage site and successfully purified as a monomer using affinity chromatography and histidine tag was cleaved with TEV protease for structural studies. Our results indicated that the Phosphatase domain showed well-defined folded structure based on data from one-dimensional and two-dimensional NMR spectroscopy. Data form circular dichroism also suggested that Phosphatase domain consisted of both ${\alpha}$ -helix and ${\beta}$ -sheet. This information will be used for detailed structural study of UBLCP1.

• 미생물학회지
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• 제54권4호
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• pp.448-452
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• 2018

제주도 성산리 앞 바다 속 해면체로부터 분리한 Bacillus safensis KCTC 12796BP의 유전체를 분석하였다. 그 결과 3,935,874 bp의 환형 염색체와 36,690 bp의 plasmid 염기 서열을 확인하였다. 염색체는 G + C 함량이 41.4%로 75개의 위유 전자를 포함한 3,980개의 코딩 서열을, plasmid는 G + C 함량이 37.3%로 36개의 코딩 서열을 포함하고 있었다. 염색체 코딩 서열 중에는 81개의 tRNA 유전자, 24개 rRNA 유전자와 1개의 tmRNA 유전자가 있었다. 또한 포자 생성에 필요한 30개의 유전자, 포자피를 지령하는 16개의 유전자, 그리고 발아에 필요한 20개의 유전자도 발견되었다. 이외에 협막 다당체 생합성에 필요한 유전자와 편모 생합성 및 주화성에 필요한 유전자, 그리고 염 내성에 필요한 glycine-choline betaine 수송체에 관한 유전자도 존재하였다. 무엇보다도 항알러지활성을 보이는 이차대사산물 seongsanamide의 생합성을 지령하는 비리보좀성 펩타이드 합성효소 유전자를 확인할 수 있었다.

• 미생물학회지
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• 제53권3호
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• pp.216-218
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• 2017

생물학적방제 효과가 뛰어난 Bacillus thuringiensis C25 균주의 유전체 분석을 수행하였다. 본 균주는 5,308,062 bp, G+C 비율 35.32%의 염색체와 308,946 bp, 32.23% G+C 함량이 포함된 plasmid를 지닌 것으로 확인되었다. 염색체와 plasmid DNA에 예측된 유전자의 총 수는 5,683개의 단백질 코딩유전자와 107개 tRNA 그리고 42개의 rRNA였다.

• 미생물학회지
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• 제54권1호
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• pp.81-83
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• 2018

Neisseria 속 균주 KEM232는 사람 평활면 치아우식 부위로부터 분리하였다. 균주 KEM232의 유전체는 G + C 비율이 58.5%, 2,369개의 유전자와 2,210개의 단백질 코딩 유전자, 108개의 위유전자, 51개의 RNA 유전자 그리고 한 개의 CRISPR array를 포함한 단일 원형 염색체로 구성되었으며 그 크기는 2,371,912 bp였다. 균주 KEM232의 최 근연종은 Neisseria baciliformis 로서 두 균주 사이의 16S rRNA 유전자 염기서열의 유사도는 96.8% 그리고 유전체의 평균 염기 동일성은 84%였다.