• 한국환경성돌연변이발암원학회지
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• 제17권1호
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• pp.17-22
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• 1997

We observed the frequency of chromosome aberrations induced by UVB irradiations, and the suppressing effect of tannic acid on chromosome aberrations induced by UVB irradiations in CHL cells, which is a phenolic compound, a hydrolysate of tannin and a components of green tea. UVB doses used for the frequency of chromosome aberrations were from 0.2 to 1.6 KJ/m$^2$ and tannic acid concentrations were from 1.16 $\mu$g/ml to 37.50 $\mu$g/ml. For the observation of suppressing effect of tannic acid on UVB-induced chromosome aberrations, UVB dose was 1.6 KJ/m$^2$ and tannic acid concentrations were 1.0, 2.0, 4.0 $\mu$g/ml. In our study, tannic acid was treated for 24 hours in CHL, cells after UVB irradiation without S9 mix or for 6 hours with S9 mix. From this study, we obtained the following results : (1) The frequency of chromosome aberrations UVB induced were dose-dependently increased. (2) The tannic acid did not induce chromosome aberrations in cultured Chinese hamster cells. (3) UVB-induced chromosome aberrations were suppressed by tannic acid at every concentration from 1.0 $\mu$g/ml to 4.0 $\mu$g/ml with or without metabolic activation. These results suggest that the tannic acid acts as an inhibitor to UVB-induced clastogenicity of the cultured cell.

• 한국환경성돌연변이발암원학회지
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• 제11권2호
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• pp.107-117
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• 1991

The effect of 3-aminobenzamide (3AB), an inhibitor of poly (ADP-ribose) polymerase, on ethyl methanesulfonate (EMS)-or bleomycin (BLM)-induced DNA repair synthesis and chromosome aberrations was examined during the cell cycle of Chinese hamster ovary (CHO)-K$_1$ cells. The synchronized cells were obtained by using thymidine double block method and mitotic selection method. Three assays were employed in this study: unscheduled DNA synthesis, alkaline elution and chromosome aberrations. 3AB alone did not induce DNA repair and chromosome aberrations in all phases. The post-treatment with 3AB inhibited DNA repair synthesis induced by EMS or BLM in G$_2$ phase, whereas 3AB did not affect chromosome aberrations induced by EMS or BLM in all phases. These results suggest that 3AB aggravates the cell cycle disturbance which occur after DNA damage, and leads to an accumulation of cells at G$_2$ phase, and inhibits DNA repair synthesis, while the effect 3AB on chromosome aberrations may need reevaluated.

• 한국산업보건학회지
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• 제5권1호
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• pp.40-47
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• 1995

We analyzed sytrene concentrations in air and in blood, mandelic acid in urine, and chromosome aberrations in peripheral lymphocytes of twenty one styrene-exposed workers in two reinforced plastic factories. In addition, in vitro testing for chromosome aberration was carried out. The dose-dependent clastogenicity of styrene was confirmed in the cultured Chinese hamster lung cell(CHL) with metabolic activation. The environmental styrene concentrations and urinary mandelic acid levels of analyzed subjects were different in two plants examined, but the exposure levels in most workers examined were lower than the permissible exposure levels. Chromosome aberrations of the styrene exposed workers showed no increase in the percentage of aberrant cells as compared with the control group. No correlation was found between the exposure levels and the frequencies of chromosome aberrations in workers.

• 한국환경성돌연변이발암원학회지
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• 제18권2호
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• pp.109-115
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• 1998

Fluorscence in situ hybridization with chromosome-specific probe has been shown to be a valid and rapid method for detection of chromosome rearrangements induced by chemical and physical agents. This method is useful for quantifying structural aberrations, expecially for stable ones, such as translocation and insertion, which are difficult to detect with conventional method in human lymphocyte. In order to use the FISH method as a biodosimeter for monitoring human population exposed to various chemical and physical agent, baseline level of chromosome rearragement was established. Blood from forty four healthy adults were collected and analysed with whole chromosome-specific probes by human chromosome 1,2 and 4. The frequencies of stable translocation were 2.45 per 100 cell equivalent and those of insertion, color juction, acentric and dicentric were 0.32, 3.28, 0.23 and 0.27 per 100 cell equivalent respectively. The frequencies of chromosome rearragements increased with age in both sexes except for dicenrics. From above result, stable aberrations accumulate with age and it may reflect integrated lifetime exposure of adverse environment.

• 한국동물학회지
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• 제19권4호
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• pp.179-186
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• 1976

DNA 回復合成과 染色體交換과의 聯關性을 추구하기 위해 알킬화제 突然變異誘發原인 MMC, MNNG, MMS를 培養한 사람의 淋巴球와 HeLa $S_3$ 細胞에 處理하여 다음과 같은 結果를 얻었다. 1. 이들 알킬화제에 의해 誘發된 DNA 回復合成은 MMC, MNNG, MMS의 濃度가 각각 $3 \\times 10^-7, 1 \\times 10^-6, 5 \\times 10^-4 M$에서 檢出되었다. 이는 MMC가 가장 强力한 DNA 回復合成 誘發原이며 다음이 MNNG 그 다음이 MMS임을 뜻한다. MMC와 MNNG는 濃度增加에 따른 DNA 回復合成에 큰 차이를 보이지 않으나 MMS는 현저한 增加를 보인다. 2. MMC에 의한 染色體異常은 濃度가 增加함에 따라 그 率에 현저한 增加를 보이나 染色體交換率에는 별차이가 없다. 그러나 MNNG는 染色體異常率에 차가 없고 染色體交換은 觀察되지 않았다. MMS는 染色體異常 및 染色體交換 모두 濃度의 增加에 따른 그 率의 增加를 나타낸다. 이러한 結果는 突然變異誘發原에 의한 DNA 回復合成이 染色體交換 및 染色體異常과 직접적인 연관성이 없음을 시사하는 것이다.

• 한국환경보건학회지
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• 제21권3호
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• pp.67-76
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• 1995

The frequencies of chromosome aberrations and sister chromatid-exchanges in peripheral blood lymphocyte of 44 nurses handling anticancer drugs were compared with those in 44 age-match controls. The frequencies of dicentric chrdmosome were $2.4\times 10^{-3}$ in the exposed and $0.5\times 10^{-3}$ in the control. The frequencies of sister cromatid exchanges in the exposed were slightly higher (5.68 SCEs/cell) than those in the control (5.04 SCEs/cell). The frequencies of chromosome aberrations and sister cromatid exchanges were not associated with duration of drug handling and types of anticancer drugs, but associated with use of safety cover.

• 한국환경성돌연변이발암원학회지
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• 제10권2호
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• pp.85-92
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• 1990

동시화시킨 CHO세포를 재료로하여 EMS 혹은 BLM에 의해 유발된 염색체 이상에 미치는 APC, ddTTP, 그리고 NOV의 효과를 조사하였다. 세포의 동시화는 thymidine block방법을 사용하였다. APC, ddTTP, 그리고 NOV의 단독처리는 염색체 이상을 유발하지 않았다. EMS에 의해 유발된 염색체 이상은 late G$_1$과 early S기에 높은 감수성을 나타내었고, BLM에 의해 유발된 염색체 이상은 G$_2$기에 가장 높은 감수성을 나타내었다. APC를 후처리할 경우 late G$_1$기와 early S기에서 EMS에 의해 유발된 염색체이상을 증가시켰다. 그러나 ddTTP나 NOV를 복합처리하기전 NOV를 전처리하면 late G$_1$기와 early S기에서의 염색체 이상이 증가되었다. 이런 결과들은 여러 가지 돌연변이원에 따라 각기 다른 세포내 반응이 유발되며 염색체 이상에서의 각 효소들의 활성 또한 다른 것으로 추측된다.

• 한국동물학회지
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• 제19권2호
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• pp.71-78
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• 1976

染色體의 構造的인 異常에 미치는 Methyl methanesulfonata (MMS)와 thymidine 相似體 (BUdR, IUdR)의 영향을 究明하기 위해 사람의 白血球 培養細胞에 2.0mM의 BUdR 또는 IUdR을 24時間 前 처리하고 이어 농도를 달리한 MMS $(0.0\\sim 2.0 mM)$를 1時間 처리한 뒤 24時間째 染色體標本을 작성하여 아래와 같은 결과를 얻었다. 1. MMS를 단독 처리한 對照區에서는 관찰된 染色體 異常型의 대부분이 예상외로 染色分體 異常型을 나타냈다. 2. BUdR 또는 IUdR의 단독처리도 染色體異常을 유발한다. 또한 MMS와 이중 처리한 경우에는 단독 처리보다 染色體異常型이 훨씬 증가한다. 따라서 thymidine 相似體는 MMS에 의한 染色體 異常型을 變化시켜주는 感受性物質로 作用함이 판명되었다.

• 한국환경성돌연변이발암원학회지
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• 제19권1호
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• pp.14-19
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• 1999

Chromosome rearrangements induced in human lymphocyte after in vitro exposure to chromium were analysed by the use of fluorescence in situ hybridization(FISH) with triple combination of composite whole chromosome-specific probe for chromosome 1, 2 and 4. Chromosome aberrations was scored by the Protocol for Aberration Identification and Nomenclature Terminology (PAINT). Stable translocation was the most frequent type of aberrations and dicentrics and insertions were also observed. Chromium treatment enhanced the frequencies of stable translocations and color junctions in a dose-dependent manners, but no distinct increase of dicentrics and insertions was seen. The ratio of the yields of translocation to the yields of dicentric varied between 13 to 27. The presents results demonstrate fluorescent in situ hybridization (FISH) is useful for detecting chromosomal rearrangements induced by chromium.

• Clinical and Experimental Reproductive Medicine
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• 제12권2호
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• pp.39-57
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• 1985

Presented in this paper the data from a chromosome study of 397 patients referred for suspected chromosmal abnormalities. Karyotypes were obtained using short-term blood culture and direct method. Of these 238 patients had normal chromosome complements; 159 (40.1%) patients had chromosome abnormality. Among all patients with chromosome abnormalities, 82.4% (131/159) had aberrations of chromosome number, the others 17.60/0 (28/159\ had aberrations of chromosome structure. Ten had a chromosome rearrangement; Five of them were reciprocal and five Robertsonian translocations. Four patients with pericentric inversions and one with paracentric inversions and four with isochromosomes were observed. There were four patients with marker chromosome, two patients had a chromosome insertion; and three others. (additional abnormal chromosomes.) Thus the results of the present study indicate the importance of cytogenetic evaluation in clinically abnormal patients.