• 농약과학회지
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• 제19권1호
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• pp.22-31
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• 2015

본 연구는 2014년도 온라인으로 판매되고 있는 농산물의 잔류농약 실태와 안전성 평가를 시행하였다. 총 124건의 농산물(엽채류 98건, 엽경채류 26건)을 2014년도 3월부터 8월까지 6차례 20곳의 온라인 쇼핑몰을 통해 무작위로 수거 및 전처리 후 GC-ECD/NPD와 LC-MS/MS를 사용하여 208종의 농약을 분석하였다. 실험 결과 깻잎, 깻순, 부추, 쑥, 열무, 대파, 취나물 7종 11건(8.9%)의 농산물에서 11종의 농약 19건이 검출되었다. 농산물 중 2건(깻순, 쑥)에서는 잔류허용기준치 이상의 농약이 검출되었다. 검출된 농약의 EDI(Estimated daily intake)와 ADI(Acceptable daily intake)를 비교하여 안전성 평가를 시행한 결과 %ADI가 100%를 넘는 대상은 없었으나 클로로타로닐이 잔류허용기준치 이상 검출된 깻순에서는 95.7%의 결과를 보이는 등 안전성 관리가 필요한 실정이었다. 본 연구결과는 앞으로 국가 농산물 잔류농약 안전성 검사 정책 결정의 기초자료로 활용될 수 있을 것으로 생각한다.

• 농약과학회지
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• 제11권3호
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• pp.144-153
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• 2007

Groundwater ubiquity score(GUS)를 이용하여 제주도 농가에서 많이 사용되고 있는 농약에 대한 지하수 오염가능성을 평가하고 등급화 하였다. 살충제 12종, 제초제 6종, 살균제 3종을 선정하여 제주도 20개 토양통에 대한 흡착특성과 농약의 이화학적 특성을 이용하여 GUS를 구하였다. 제주도 토양에서 조사된 각종 농약의 전반적인 지하수 오염가능성은 제초제 > 살균제 > 살충제 순서이었다. 제초제의 지하수 오염가능성의 크기는 bromacil > metolachlor > alachlor > linuron pretilachlor > butachlor 순서로 나타났다. 살충제의 지하수 오염가능성의 크기는 carbofuran > ethoprophos > diazinone > dimethoate > penthoate > mecarbam > methidathion > endosulfan > fenitrothion > parathion > chlorpyrifos > terbufos 순서로 나타났다. 살균제의 지하수 오염가능성의 크기는 metalaxyl > chlorothalonil > triadimefon 순서로 나타났다. 이들 농약 중에서 특히 지하수 오염가능성이 높은 것으로 분류된 것은 제초제 alachlor, metolachlor, bromacil, 살충제 ethorophos와 carbofuran 그리고 살균제 metalaxyl이었다. 농약에 의한 지하수 오염을 방지하기 위해서는 농약의 절대 사용을 줄일 수 있는 방안을 마련함과 동시에 지하수 오염가능성이 낮고 오염시킨 예가 적은 약제의 선별적 사용이 바람직할 것이다. 그리고 특히 오염가능성이 매우 높은 것으로 평가되고 오염의 예가 많은 약제에 대해서는 적극적인 사용제한 방안을 마련할 필요도 있을 것이다.

• Journal of Ginseng Research
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• 제22권4호
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• pp.223-228
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• 1998

As part of a study on the ability of fungicides to control disappearing root rot of ginseng (Panax quinquvdius) caused by Cylindruarpn destmtans, 15 fungicides were screened for toxicity to the fungus in vitro. Highly toxic fungicides were Benlate (benomyl), Thiram (thiram), and Orbit (propiconazole). EC5O values (mg a.i./L) were less than 1 and EC95 values were less than 10. Crown (carbathiin and thiabendazole), ASC-66835 (fluazinam), and UBI-2584 (tebuconazole) were moderately toxic, with EC5O values in the range 1-10 and EC95 values in the range 32-45. Weakly toxic fungicides (EC5O in the range 20-80, EC95 in the range 35-140) included UBI-2643 (thiabendazole), UBI-2565 (cyproconazole), and Vitaflo-280 (carbathiin and thiram). Anvil (hexaconazole), Vitaflo-250 (carbathiin), UBI-2383 (triadimenol), Daconil (chlorothalonil), CGA-173506 (fludioxonil), and CGA-169374 (difeno- conazole) were considered nontoxic to C. destmtan (EC5O 1.29->600, EC95>500). Relations between proportional inhibition of growth and concentration of fungicide were linear on arithmetic plots (Benlate, UBI-2643, UBI-2565, Vitaflo-280) or logarithmic plots (all other fungicides). Based on toxicity in vitro and formulation, it is recommended that Benlate, Orbit, and ASC-66835 be tested as soil drenches, and Benlate, Thiram, UBI-2584, and Crown be tested as seed treatments for controlling disappearing root rot.

• The Plant Pathology Journal
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• 제24권4호
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• pp.447-452
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• 2008

Four hundred and sixty six isolates of B. cinerea were obtained from infected leaves, stems and fruits of paprika grown in greenhouses or plastic film houses in Gangwon province, Korea, between August and November in 2006 and 2007. These isolates were classified into five representative phenotypes of resistant (R) and sensitive (S) reactions as SSR, SRR, RSS, RRS and RSR according to the responses of isolates against benzimidazole, dicarboximide and N-phenyl-carbamate fungicide in order. The percentage of five phenotypes were 51.3, 2.4, 35.6, 8.1 and 2.6%, respectively. The SSR phenotype (51.3%) was the most common. Among the nineteen fungicide products evaluated to compare their efficacy against gray mold pathogen on the paprika fruit inoculated with fungal mycelia, the mixture of diethofencarb and carbendazim was the most effective followed by iprodione, boscalid, the mixture of iprodione and thiophanate-methyl, fludioxonil, polyoxin-B, fluazinam, the mixture of tebuconazole and tolyfluanid and procymidone; while in the assay methods inoculated with fungal spores, the mixture of tebuconazole and tolyfluanid was the most effective in controlling gray mold followed by boscalid, fludioxonil, the mixture of diethofencarb and carbendazim and the mixture of pyrimethanil and chlorothalonil.

• The Plant Pathology Journal
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• 제18권5호
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• pp.271-278
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• 2002

The effect of five plant population densities [5 (D$_1$), 10 (D$_2$), 20 (D$_3$), 30 (D$_4$), and 40 (D$_{5}$) plants/m$^2$] of four groundnut cultivars [ICGV 86699, ICG (FDRS) 10, ICGS 11 and TMV 2] and fungicide application (Kavach, chlorothalonil) to manage late leaf spot (LLS) and rust were studied in a field experiment during the 1995 and 1996 rainy seasons. LLS and rust severities were low in fungicide sprayed plots in all the cultivars irrespective of plant densities. Severities of LLS and rust, and percentage defoliation caused by LLS were significantly more in higher plant densities (D$_4$, D$_{5}$) than in lower plant densities (D$_1$, D$_2$, D$_3$) in fungicide sprayed and unsprayed plots in all the cultivars. All the cultivars gave significantly higher haulm and pod yields in fungicide sprayed plots than in unsprayed plots. Haulm and pod yields were significantly higher in higher plant densities than in lower plant densities. A combination of higher plant densities (D$_4$, D$_{5}$) and fungicide protection against LLS and rust gave maximum yield.yield.

• 한국식물병리학회지
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• 제10권4호
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• pp.284-291
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• 1994

In order to establish an appropriate spray schedule to reduce the infection, 11 kinds of chemicals were tested for their properties on the inhibition of spore germination at the surface of apple fruits and the duration of the inhibitory effect after spray of each chemical was examined from late June to early September with basically 10 day intervals. Actual control efficacy of each chemical by the 8 successive spray and the patterns of waterborne spore dispersals during that periods were also examined. Combining those results with the meteorological observation data, actual control efficacy of each chemical in the given periods could be estimated. It was revealed that folpet, Brodeaux mixture, mancozeb, oxine copper and imminoctadine-triacetate could be used at any time during the possible infection periods. Captan and dithianon could also be used except the rainy season due to the short duration of inhibitory efficacy against spore germination under heavy rain. However, the usefulness of propineb, benomyl and chlorothalonil against the apple white rot could not be demonstrated in this experiment. Thiram, even though has not been used for apple white rot, can also be used before or after the rainy season to control not only white rot but also alternaria blotch.

• Mycobiology
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• 제35권1호
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• pp.39-43
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• 2007

The antifungal effect of pine needle extract prepared by a distinguishable extraction method and the dry distillation method, was examined. The effect of this extract itself was insignificant. The chemical components of pine needle extract were then investigated by gas chromatographic analysis, and four, chemical components, acetol, furfural, 5-methyl furfural, and terpine4-ol, were identified. The antifungal effects of those four chemical components against Alternaria mali (A. mali), an agent of Alternaria blotch of apple, were then examined. It was observed that the minimum inhibitory concentrations (MICs) were 6.25, 0.78, 0.78, and 12.5 (mg/ml) of acetol, furfural, 5-methyl furfural, and terpine-4-ol, respectively. MICs of furfural and 5-methyl furfural had the same order of magnitude as that of an antifungal agrochemical, chlorothalonil. Although furfural itself can not be completely substituted for an antifungal agrochemical, a partial mixture of furfural and antifungal agrochemical may be used as a substitute. The use of agrochemicals for the prevention of plant disease caused by pathogenic fungus such as A. mali could be partially reduced by the application of this mixture.

• The Plant Pathology Journal
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• 제22권1호
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• pp.75-89
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• 2006

The actinomycete strain JK-1 that showed strong inhibitory activity against some plant pathogenic fungi and oomycetes was isolated from Jung-bal Mountain in Ko-yang, Korea. The strain JK-1 produced spores singly borne on sporophores and the spores were spherical and 0.9-1.2 11m in diameter. The cell wall of the strain JK-1 contained meso-diaminopimelic acid. The actinomycete strain JK-1 was identified as the genus Micromonospora based on the morphological, physiological, biochemical and chemotaxonomic characteristics. From the 168 rDNA analysis, the strain JK-1 was assigned to M aurantiaca. The antibiotic MA-1 was purified from the culture broth of M aurantiaca JK-1 using various purification procedures, such as Diaion HP20 chromatography, C18 flash column chromatography, silica gel flash column chromatography and Sephadex LH-20 column chromatography. $^{1}H-$, $^{13}C-NMR$ and EI mass spectral analysis of the antibiotic MA-1 revealed that the antibiotic MA-1 is identical to phenylacetic acid. Phenylacetic acid showed in vitro inhibitory effects against fungal and oomycete pathogens Alternaria mali, Botrytis cinerea, Magnaporthe grisea, Phytophthora capsici and yeast Saccharomyces cerevisiae at < 100 $\mug$ $ml^{-1}$. In addition, phenylacetic, acid completely inhibited the growth of Sclerotinia sclerotiorum, Bacillus subtilis, Candida albicans, Xanthomonas campestris pv. vesicatoria at < $\mug$ $ml^{-1}$. Phenylacetic acid strongly inhibited conidial germination and hyphal growth of M grisea and C. orbiculare. Phenylacetic acid showed significantly high levels of inhibitory' effect against rice blast and cucumber anthracnose diseases at 250 $\mug$ $ml^{-1}$. The control efficacies of phenylacetic acid against the two diseases were similar to those of commercial compounds tricyclazole, iprobenfos and chlorothalonil .n the greenhouse.

• 대한의생명과학회지
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• 제22권4호
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• pp.199-206
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• 2016

Pesticides are widely used to prevent loss of agricultural production but extensive exposure can induce health problems to pesticide operators. This study was performed to evaluate the health risk of highly produced pesticides used in fruit growing farm land by comparison of estimated exposure level with AOEL using KO-POEM program. AOEL was driven based on NOAEL of each pesticide evaluated by JMPR, EFSA or KRDA. In calculation of exposure level, types of formulation, dilution factors, spraying duration and motor type and exposure protection device were allocated according to actual condition of use. Dermal absorption rate was differently applied among EFSA default values (25% or 75%), general default value (10%) or real test result values to know the plausibility of default values and safety of pesticide to operators in outline. Twenty pesticide ingredients (fungicides and insecticides) were produced more than 30 tons per year, which were mancozeb, chlorothalonil, imidaclopirid and etc in order. Dermal absorption rates obtained from studies were various from 0.07 to 81% but mostly under 10%. The estimated exposure levels showed big differences more than 10 times higher when using EFSA default rate and up to 5 times higher when using general rate of 10% comparing using rates of test results. Mancozeb, chlorthalonil, diazinon and chlorpyrifos presented still higher exposure level than AOEL even when using test absorption rate from study, which suggests that re-evaluation of AOEL or dermal exposure absorption rate or strict management are required for health protection of operators who use those four pesticides in farm land.

• 한국식품위생안전성학회지
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• 제24권4호
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• pp.338-347
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• 2009

The content of residual pesticides in commercial agricultural products in Gyeongsangbuk-Do area was investigated for 5 years extending the year 2004 through 2008. The detection rates of residual pesticides in agricultural products by year were similar in range of 11.6~16.4%. But the violation rates showed lower values in the last years from 4.5% of the year 2004 to 0% of the year 2007. The highest residual concentration of each pesticide detected in commercial agricultural products was investigated by year. That is, in the year 2004 and 2005, chlorpyrifos, chlorothalonil, diazinon, endosulfan, ethoprophos, fenarimol and procymidone were detected over the tolerance in kale, parsley, celery, chard and lettuce, and in the year 2006, permethrin in the soybean and peanut. The detection rate and violation rate of pesticides were highly increased in the order of the endosulfan, chlorpyrifos, procymidone, chlorfenapyr, fenitrothion, imazalil, isoprothiolane, methidathion and permethrin. The detection rate and violation rate of pesticides were increased after August every year.