• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.2
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• pp.288-295
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• 1998

Egg production of Acartia steueri was estimated in llkwang Bay, located in the southeastern coast of Korea. The equation, combining fecundity, temperature and chlorophyll a, obtained under the laboratory experiments, was applied to the in situ temperature and chlorophyll a for the estimation of field egg production. Mean egg size was $80.52{\mu}m$. Prosome length of adult females was not correlated with egg size. Egg production of field population ranged from 0.32 to $63.32{\mu}gC\;m^{-3}d^{-1}$ with a mean of $13.33{\mu}gC\;m^{-3}d^{-1}$, which were equivalent to $7.1\~1407.1\;eggs\;m^{-3}d^{-1}$ and $296.1\;eggs\;m^{-3}d^{-1}$, respectively. Fecundity of adult females ranged from 5.4 to $12.5\;eggs\;female^{-1}d{-1}$ with a mean of $8\;eggs\;female^{-1}d^{-1}$. Specific egg production rates ranged from 0.028 to 0.117 $d^{-1}$ with a mean of 0.064 $d^{-1}$. Considering the egg Production ($\%$ female body carbon) as a function of temperature and chlorophlrll a concentration, our results showed rather low fecundity, which might be underestimated. Probable cannibalism of egg by the adults were thought to be pan of the reason for this low estimated fecundity.

• Journal of Korean Society of Forest Science
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• v.106 no.1
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• pp.33-39
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• 2017

This study was carried out in order to closely examine the growth and the physiological response by shading levels of a container seedling of Daphniphyllum macropodum which is known as the species of having shade tolerance in a warm temperature evergreen broad-leaved tree. The shading treatment was regulated with the shading level of full sunlight, and 35%, 55%, 75% of full sunlight. As a result of surveying the height and the root collar diameter growth by the shading level, the height was the highest with 13.2 cm under 35% of shading. The root collar diameter was the highest with 4.99 mm under full sunlight. The organ-based (leaves, shoot and root) dry mass production and the whole dry mass production were higher with 1.38 g, 0.47 g, 0.87 g, 2.72 g, respectively, in order of 35% shading. As for chlorophyll a, b, and the total chlorophyll content, the highest chlorophyll content was shown under 75% of shading with the relatively highest shading level. The ratio of chlorophyll a to chlorophyll b (a/b) was the highest under 35% of shading. The photosynthetic rate, stomatal conductance and water use efficiency were the highest with $12.3{\mu}molCO_2{\cdot}m^{-2}{\cdot}s^{-1}$, $0.27molH_2O{\cdot}m^{-2}{\cdot}s^{-1}$, $3.53{\mu}molCO_2{\cdot}mmol^{-1}H_2O$, respectively, under 35% of shading. As a result of surveying the whole experiment, D. macropodum grows nicely by maintaining 35% shading.

• Journal of Bio-Environment Control
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• v.27 no.3
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• pp.231-238
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• 2018

Chlorophyll fluorescence, chlorophyll content, graft-taking and growth of grafted cucumber seedlings as affected by photosynthetic photon flux (PPF) of LED lamps were analyzed in this study. Four PPF levels, namely 25, 50, 100, $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ were provided to investigate the effect of light intensity on the chlorophyll fluorescence, chlorophyll content, graft-taking and growth of grafted cucumber seedlings. Air temperature, relative humidity, and photoperiod for graft-taking were maintained at $25^{\circ}C$, 90%, $16h{\cdot}d^{-1}$, respectively. Maximum quantum yield (Fv/Fm) of rootstock as affected by PPF was found to be 0.84-0.85 and there was no significant change in Fv/Fm. Even though Fv/Fm of scion measured at 2 days after grafting was lowered to 0.81-0.82, after then it gradually increased with increasing PPF. At 4 days after grafting, the chlorophyll content extracted from scion increased with increasing PPF. Graft-taking ratio of grafted cucumber seedlings was 90-95% as PPF was ranged from $25{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ to $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. However, the graft-taking ratio of grafted seedlings healed under PPF of $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ was decreased to 80%. Maximum PPF measured required for smooth joining of rootstock and scion was assumed to be $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. At healing stage of grafted cucumber seedlings, Fv/Fm of scion decreased and at least two days after grafting were required for rooting of grafted seedlings. Chlorophyll fluorescence response of rootstock and scion was linked to light irradiation. Therefore, it was concluded that physical environment including light and humidity during healing process of grafted seedlings should be controlled more precisely to facilitate root formation and to prevent scion from lowering Fv/Fm. Further studies are required to investigate the effects of root development and joining of vascular bundles of grafted seedlings on the chlorophyll content of scion.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.818-829
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• 2016

This study evaluated the responses of 18 potato cultivars to three levels of salinity stress (electrical conductivity, EC: 1.0, 4.0, and $8.0dS{\cdot}m^{-1}$). Stem, leaf, root, chlorophyll, tuber yield, and proline content were investigated and statistically analyzed using analysis of variance (ANOVA) and correlations. Stem number and stem diameter were not affected by salinity, but stem length and aerial weight showed highly significant responses to salinity. Aerial weight decreased with increasing salinity levels in most cultivars, while it increased in some the cultivars 'Daejima', 'Goun', 'Haryeong', and 'LT-8'. Leaf number, leaf area index, and leaf weight were most significantly affected by salinity and the cultivar ${\times}$ salinity interaction. Root length, root weight, total chlorophyll and chlorophyll a were affected by salinity, but not by the cultivar ${\times}$ salinity interaction. The opposite trend was shown in chlorophyll b. Although there was great variability among cultivars, tuber yield decreased in all cultivars, and was most significantly influenced by salinity and the cultivar ${\times}$ salinity interaction. 'Superior', 'Kroda', 'Romana', and 'Duback' gave better tuber yields under salinity at EC 4.0 and $8.0dS{\cdot}m^{-1}$ than the cultivars with better aerial weights. Proline content was increased by salinity in all cultivars, and was more remarkable in the cultivars with better aerial weights than in cultivars such as 'Superior' and 'Kroda' with better tuber yields. Leaf number, leaf area index, leaf weight, and root length parameters were considered to be useful criteria in the evaluation of salt tolerance because of their high positive correlation with tuber yield; however, given its negative correlation with tuber yield under high salinity, proline content was not. Salinity tolerances varied greatly among potato cultivars. The low correlation between growth and yields of aerial parts under high salinity suggests that, in commercial agriculture, it might be more practical to compare relative yields to controls. Additionally, 'Superior', 'Kroda', 'Romana', and 'Duback' might be very useful cultivars to use in breeding programs to develop salinity-tolerant potatoes, as well as for sustainable potato production in saline areas.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.94-100
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• 2003

We studied the effect of cadmium on chlorophylls and rubisco activation in Canavalia ensiformis L. leaves. Chlorophyll levels were reduced by 5.0 ${\mu}$M Cd. Rubisco activity at 5.0 ${\mu}$M Cd was significantly smaller than that at no treatment. Rubisco Content showed patterns of change similar to rubisco activity. These data suggest that rubisco activity was associated with an amount of rubisco protein, and that the activation and induction of rubisco is inhibited by Cd. The degree of intensity of 50 and 14.5 kD polypeptides identified as the large and small subunit of rubisco by SDS-PAGE analysis at 5.0 ${\mu}$M Cd was significantly lower than that at control, indicating Cd had a e f-fect on both subunits. Under the assumption that effects of Cd on rubisco may be r elated to rubisco activase, in addition to, its activity and content we re determined . The rubisco activase activity at 5.0 ${\mu}$M Cd was more decreased than the control. A similar change pattern was also observed in content of rubisco activase. Remarkable differences in the intensitiy of both the 45 kD and 41 kD band were found between at control and Cd-treatment. These results suggest that the change in the levels of rubisco activase leads to a subsequent alter action of rubisco levels.

• Proceedings of the KSRS Conference
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• v.1
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• pp.495-498
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• 2006

Optical profile and spectral absorption of suspended solids in waters of Sanya bay was measured on August 8-14, 2003. Optical profile was taken by using MicroPro optical profile. Apparent optical indexes, vertical diffuse attenuation coefficient ($K_d$) and water leaving radiance (Lw), were calculated. $K_d$ at the blue end of the spectrum was greater than that at the red end of the spectrum in waters near Sanya River mouth, however, in waters near open sea, $K_d$ at the blue end of the spectrum was smaller than that at the red end of the spectrum. Distribution of water leaving radiance was relatively higher in waters near Sanya River mouth, but relatively weaker in near open sea water. Spectral absorption of suspended particulates was also measured. Results showed that the spectral absorption of chlorophyll a was greater in waters near Sanya river mouth, but relatively weaker in waters near open sea, which indicated higher concentration of phytoplankton in waters near Sanya river mouth. Except for water at the 5th sampling station, the ratio of spectral absorption of chlorophyll a to total suspended particulates in surface waters was greater than that in bottom waters at all stations.

• Asian Journal of Turfgrass Science
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• v.11 no.1
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• pp.45-58
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• 1997

Pigments and thylakoid membrane proteins were investigated in wild type and PS I- mutants from Synechocystis sp. PCC6803 Comparing morphological features, B2 was less fluorescent than the other strains. The contents of chlorophyll a were propotional to the FNR activity in thylakoid membrane. The FNR activity of mutants was lower than that of wild type. In the result of pigments analysis, mutants had smaller cholophyll a than that of wild type. The major carotenoid was found to he $\beta$-caroene, but aeaxanthin was barely detected in thylakoid membrane of mutants. The polypeptide, 14.8kD was detected by electrophoresis in mutants. It was considered to be the modification of 15.4kD in wild type. Membrane polypeptides of 17.6 and 19.7kD were not detected in mutants. In the result of western blotting, subunit I was detected in all strains, but subunit II was barely detected in mutants. Subunit II was not detected in B2 at all. In view of the results so far achieved, the changes of contents of chlorophyll and zeaxanthin were affected by the defficiency or modification of functional domain in subunit I. Also the modification in subunit I affected the subunit II- binding site in PS I. As the result, efficiency of photosynthesis was decreased. Key words: Synechoystis sp. PCC6803, PS I - mutant, Photosynthetic efficiency, Pigment,Thylakoid membrane proteins, Subunit I, II.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.3
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• pp.268-274
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• 2010

The aim of our study was to investigate the interactive effects of container size and nutrient supply on plant growth, chlorophyll synthesis, transpiration, $CO_2$ assimilation, water use efficiency (WUE), and nutrient uptake of vinca plant (Catharanthus roseus). A complete experiment utilizing four concentrations of fertilizer and three volumes of containers was conducted. As the container size was increased, the plant height, leaf area, and dry weight of vinca significantly increased regardless of fertilizer level. The leaf area and dry weight of vinca were highly sensitive to the container size. However, the chlorophyll contents of vinca 20 days after the transplant significantly increased with decreasing container sizes and increasing fertilizer concentrations. Significant differences in transpiration and $CO_2$ assimilation occurred with the use of differentfertilizer solutions, but the highest values for transpiration and $CO_2$ assimilation were in plants grown in the 15 cm-diameter containers. The highest water use efficiency was observed in the plants grown in 10 cm-containers with 4 dS/m of fertilizer, and there were no significant differences in WUE values among container sizes with fertilizer concentrations of 0, 1, or 2 dS/m. No significant difference in nutrient uptake was observed among the fertilizer levels or among the container sizes. However, at a fertilizer concentration of 4 dS/m, the uptake of several nutrients, including N, P, K, Ca, Mg, B and Fe, was higher in small containers than in larger ones.

• Journal of Bio-Environment Control
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• v.12 no.4
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• pp.235-239
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• 2003

This study was carried out to investigate a adequate strength of nuttient solution in production of marjoram and oregano by hydroponicsl Two herbs were grown for 40days with the strength of 0.5, 1.0, 2.0, and 3.0 dS${\cdot}m^{-1}$ in herb's nutrient solution developed by European Vegetable R & D Center, Belgium. the growth, vitamin C, and essential oil content of marjormam were higher in the treatment of 0.5 dS${\cdot}m^{-1}$ than the other treatments tested. But marjoram growtn with the sstrength of 20. and 3.0 dS${\cdot}m^{-1}$ had the highest mineral contents and essential oil content, respectively. Elevated strength of nutirent solution decreased chlorophyll content. In oregano, the growth was vest in the stength of 0.5, but withered in the strength of 3.0 The chlorophyll content was the lowest in the strength of 0.2dS${\cdot}m^{-1}$, while vitamin C contents had no statistical differences among treatments, Oregano grown with 1.0 dS${\cdot}m^{-1}$had the highest mineral and essential oil content, but the oil yield washighest in the treatment of 0.5 dS ${\cdot}m^{-1}$. In conclusion, the strength of 0.5 dS${\cdot}m^{-1}$ herb's nutrient solution was adequated in production of marjorma and oregano in hydroponicc system.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.63-68
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• 2002

The possibility for mass production of phytol, inhibitory diterpene against ACAT (Acyl-CoA: Cholesterol acyltransferase) was investigated by using callus culture of lettuce. The callus were induced from lettuce cotyledon explants on MS medium containing 0.5 mg.L$^{-1}$ NAA after 4 week's culture. Adventitious roots were formed from the explants on MS medium containing 0.5 mg.L$^{-1}$ IBA or 1.0 mg.L$^{-1}$ NAA. Adventitious shoots and roots were emerged from the callus when the callus was transferred to MS medium containing auxin alone, or with cytokinin. The plant growth regulators and their concentrations for the organogenesis were 1.0 mg.L$^{-1}$ NAA, 0.1 mg.L$^{-1}$ BA, 0.5 mg.L$^{-1}$ NAA with 0.1 mg.L$^{-1}$ kinetin, or 0.5 g.L$^{-1}$ 2.4-D with 1.0 mg.L$^{-1}$ kinetin. Analyses of chlorophyll contents showed that chlorophyll contents were higher in morphogenic calli than in non-morphogenic calli. However, the chemical analyses of gas chromatography indicated that phytol contents were not proportionate to the chlorophyll contents of callus. The content of phytol was higher in callus than in lettuce cotyledon.ledon.