Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Production of Phytol, an ACAT Inhibitor, from Callus Culture of Lettuce (Lactuca sativa L.)

상추 (Lactuca sativa L.) callus로부터 ACAT 억제 활성물질, phytol의 생산

  • An, Kwang-Hee (Department of Agronomy, Plant Metabolism Research Center, Kyunghee University) ;
  • Jang, Tae-O (Department of Agronomy, Plant Metabolism Research Center, Kyunghee University) ;
  • Baek, Nam-In (Department of Agronomy, Plant Metabolism Research Center, Kyunghee University) ;
  • Kim, Se-Young (Department of Agronomy, Plant Metabolism Research Center, Kyunghee University)
  • 안광희 (경희대학교 농학과, 식물대사연구센터) ;
  • 장태오 (경희대학교 농학과, 식물대사연구센터) ;
  • 백남인 (경희대학교 농학과, 식물대사연구센터) ;
  • 김세영 (경희대학교 농학과, 식물대사연구센터)
  • Published : 2002.03.01
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Abstract

The possibility for mass production of phytol, inhibitory diterpene against ACAT (Acyl-CoA: Cholesterol acyltransferase) was investigated by using callus culture of lettuce. The callus were induced from lettuce cotyledon explants on MS medium containing 0.5 mg.L$^{-1}$ NAA after 4 week's culture. Adventitious roots were formed from the explants on MS medium containing 0.5 mg.L$^{-1}$ IBA or 1.0 mg.L$^{-1}$ NAA. Adventitious shoots and roots were emerged from the callus when the callus was transferred to MS medium containing auxin alone, or with cytokinin. The plant growth regulators and their concentrations for the organogenesis were 1.0 mg.L$^{-1}$ NAA, 0.1 mg.L$^{-1}$ BA, 0.5 mg.L$^{-1}$ NAA with 0.1 mg.L$^{-1}$ kinetin, or 0.5 g.L$^{-1}$ 2.4-D with 1.0 mg.L$^{-1}$ kinetin. Analyses of chlorophyll contents showed that chlorophyll contents were higher in morphogenic calli than in non-morphogenic calli. However, the chemical analyses of gas chromatography indicated that phytol contents were not proportionate to the chlorophyll contents of callus. The content of phytol was higher in callus than in lettuce cotyledon.ledon.

상추의 기내 callus 배양으로부터 ACAT (Acyl-CoA: Cholesterol acyltransferase) 억제 활성물질로 알려진 phytol 의 생산가능성이 연구되었다. Callus는 0.5mg.L$^{-1}$ NAA가 첨가된 MS 배지에 4주간 치상된 자엽 절편체로부터 유도되었으며, 다양한 수준의 chlorophyll을 함유하고 있었다. Phytol이 chlorophyll분자를 구성하는 물질로 알려져 있음에 도 불구하고, phytol의 추출량은 녹색의 자엽에서 보다 callus 에서 높게 나타났다. GC 분석결과, phytol의 추출량은 callus의 chlorophyll 함량과 비례하지 않는 것으로 나타났으며, callus의 chlorophyll 함량수준은 callus의 형성능과 관련이 있는 것으로 사료되었다. 본 연구의 결과는 phytol의 기내생산에 있어서 상추의 callus 배양의 잠재성을 제시한다 하겠다.

Keywords

References

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