• Journal of Plant Biology
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• v.26 no.2
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• pp.91-99
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• 1983

The formation of chlorophyll-protein complexes (CP-complexes) during the greening of rape cotyledons (Brassica napus cv. Yongdang) was investigated by the SDS-polyacrylamide gel electrophoresis. The total chlorophyll content and Chl a/b ratio were also determined. In addition, the effects of dark treatment on the CP-complex patterns during greening have been examined with respect to their photosynthetic electron transport activity. Greening has brought about the increasein total chlorophyll content and the decrease in Chl a/b ratio, but there have been no changes in Chl a/b ratio after 24 hrs of greening. The light-harvesting chlorophyll a/b-protein complex (LHCP-complex0 was predominant during the initial greening period. Thereafter, the amout of chlorophyll a-protein complex (CP I-complex) was gradually increased. Twenty-four-hr dark treatment immediately after illumination for 6 hrs and 12 hrs resulted in the increase of the Chl a/b ration and the CP I complex, otherwise the decrease of the LHCP-complex. The LHCP/CP I ratio was gradually decreased with further greening, and appeared no change after 48 hrs illumination. The investigation of the photosynthetic electron transport activity indicated that photosystem (PS) II activity (H2Olongrightarrowp-PD*＋FeCy**) did not change, but the activity of PS I was increased suddenly due to the dark treatment. The data suggests that the increase of CP I-complex may result in that of P-700, that is, the increase of PS I activity.

• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.3
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• pp.159-163
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• 2002

This study was analyzed to photosynthetic pigment concentrations and changes of SOD activities on seven species of liana of A. heterophylla, P. scandens, V. thunbergii, P. tricuspidata, C. trilobus, L. japonica and T. kirilowii, and two species of E. arvense and A. princeps of non climbing plants. Concentrations of chlorophyll a and b, total chlorophylls and total carotenoids of P. tricuspidata in 100 ppb ozone site were the most increased. It was the most increased to P. scandens in ratio of chlorophylls and carotenoids, and E. arvense in ratio of chlorophyll a and b. There was difference to ratio of chlorophyll a and b of liana and non liana. At ratio of chlorophyll a and b of 100 ppb ozone site and the control it was more sensitive to chlorophyll a than chlorophyll b, and P. tricuspidata was the most sensitive at comparing with species, and it was more sensitive to liana than non liana. In SOD activities A. princeps was the most increased to 3535.7 unit/g, and P. scandens was the fewest increased to 109.3 unit/g, and A. heterophylla was only decreased to 131.7 unit/g in comparing to 100 ppb ozone sites and the control.

• Journal of the Korean Chemical Society
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• v.26 no.5
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• pp.304-309
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• 1982

The fluorescence yields of chl-a and-b complexes bound to silicon dimer, tetramer and hexamer containing pyridine group in diethyl ether solvent, were shown the transition of excited energy through silicon polymer chain, and the maximum energy quenching were appeared at 1 : 1 ratio of chl-a and pyridine group in silicon polymer but the chl-b complexes were shown the maximum energy of fluorescene emission at the same ratio.

• Applied Biological Chemistry
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• v.39 no.4
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• pp.293-296
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• 1996

The present study was carried out to investigate the effect of Ni on germination, cell elongation, ${\alpha}-amylase$ activity, contents of chlorophyll and protein in radish were determined in the water culture. As the concentration of Ni was increased in the water culture, germination of radish was 55% by Ni 10 mg/kg and 30% by Ni 20 mg/kg. The ratio of cell elongation injury was 50%, by two days after Ni 20 mg/kg treatment. The injury ratio of ${\alpha}-amylase$ activity was 45% in the same condition and as the time goes on, inhibition of ${\alpha}-amylase$ activity were slightly decreased. Contents of chlorophyll a and b were decreased two days after treatment and chlorophyll a was more inhibited than chlorophyll b. Also changes of the protein contents was slightly decreased. Activity of ${\alpha}-amylase$ was decreased at germination stage, contents of chlorophyll a and b were decreased at growing stage.

• Journal of the Korean Professional Engineers Association
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• v.18 no.4
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• pp.12-22
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• 1985

This investigation was attempted to determine changes in chlorophyll. Pheophytin content PH and titratable acidity during young redish Kim chi fermentation. Young redishes divied in to leaf and vein and fermented in dark room for 35 days at 10$^{\circ}C$ after pickling each group. Samples were collected at 5 days interval for the chemical analysis. The following results were obtained. 1. Chlorophyll content in young redish was decreased by decreasing PH during fermentation periods. Chlorophyll content decreased from 11.60mg/g to 7.62mg/g in leaf, from 0.48mg/g to 0.33mg/g in vein respectively during 35 days fermentation periods. In contrast to chlorophyll. pheophytin content increased as chlorophyll content decreased during the period. The ratio of chlorophyll a and b content in young redish about 3 : 1 in leaf and vein and decreased nearly the same ratio during fermentation periods. In case of pheophytin a and b it was increased without any relation with the ratio chlorophyll a and b. In generally, the optimum PH for eating was known 4.3 to 5.0. Young redish Kim chi was reached the optimun PH at 15 to 25 days of fermentation at 10$^{\circ}C$. At this periods. Chlorophyll content varied from 11.32mg/g to 9.30mg/g for leaf and from 0.43mg/g to 3.37mg/g for vein, respectively. 2. Titratable acidity(as lactic acid) was increased to 0.54% in leaf at 20 days fermentation period during Kim chi fermentation. and nearly unchanged until 30days. At the end of fermentation period the titratable acidity was about 0.30％. Titratable acidity was increased as decreasing pH. and the correlation coefficients was -0.92 in leaf and -0.77 in Vien. 3. Correlation between titratable acidity and chlorophll, pheophytin content was not significant statistically.

• Journal of Life Science
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• v.29 no.5
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• pp.509-513
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• 2019

The Beer-Lambert law states that absorbance is proportional to the concentration of a solute in a solution at a given wavelength. This linearity works for an ideal or a 'sufficiently diluted' solution, so this linearity is often used as a criterion for the fidelity of the absorbance value measured. In this study, we used a chlorophyll (Chl) solution, isolated from rice leaves with 80% acetone to test the use of the Chl a/b ratio as an additional criterion for checking the fidelity of measured values using four different absorption spectrophotometers: Cary4E, UV-1650PC, Versamax (a microplate reader), and NanoDrop 1,000(which can handle a $4{\mu}l$ aliquot). We used Chl solutions of varying concentrations from $0.2{\mu}g/ml$ to $200{\mu}g/ml$ to measure absorbance values at 645 nm and 663 nm and checked the linearity first. The results indicated that the range of Chl concentrations that we can rely on based on the linearity was similar to the range in which the calculated Chl concentrations based on the measured absorbance values agreed with the known concentrations. However, some border cases or cases with very low Chl concentrations inside the fidelity range of Chl concentrations did not agree with the criterion that the Chl a/b ratio should not change after dilution of the Chl in the solution. These results suggest that the Chl a/b ratio is a better criterion for the reliability of the absorbance values measured for the determination of chlorophyll concentration than the criterion based on the linearity suggested by the Beer-Lambert law.

• Journal of Environmental Science International
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• v.5 no.5
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• pp.643-656
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• 1996

Various light intensity and light quality were treated to oat seedlings to investigate the effect of light on the chlorophyll accumulation and the formation of chlorophyll-protein complexes. The Increase of total chlorophyll accumulation and Chl (chlorophyll) a/b ratio was promoted under H (high intensity) white light during oat chloroplast development when compared to 1 (low intensity) white light. Also H white light was more effective in the formation of chlorophyll-protein complexes associated with PSI CCI and CCII than L white light. The seedlings grown in various right quality caused little changes in total chiorophyl and ChI-a/b ratio when compared to those grown in L white light. The assembly of LHCII trimer was more affected by L white light treatment in the formation of-chlorophyll-protein complexes than red light treatment. The effect of blue light on the relative composition of chlorophyll-protein complexes was similar to that of L white light. Particualrly, blue light was more effective in the synthesis of LHCII monomer than the other light quality at the early stage of greening. When compared to red light, blue light was more effective the increase of chlorophyll accumulation and Chl a/b ratio than 1ight Quality, and light Quality may be in important factor for the regulation of the organization in the chlorophyll-protein complexes curing greening.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.447-456
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• 2013

This study was carried out for the establishment of theory and foundational data for breeding, on variegated foliage plants using for potted plants, based on examination and analysis for shape, color, and area ratio of variegated leaves and chlorophyll contents. Six families, 18 genus, and 54 species of variegated plants domestically distributed in Korea were used as the plant materials. Patterns based on chlorophyll-deficient part in variegated leaves were divided into 20 types, such as steps, border, collapsed border, sandy border, half, silk, stars, vein, firewood, border and firewood, center, entirety, leaf, irregular, net, watermelon, melon, and so on. There were 10 kinds of colors including dark green, red, dark red, reddish white, reddish yellow, white, silver, silvery white, yellowish white, and yellow. The ratio of variegated area was ranged from 5.8% to 100% and it was diverse depending on species or cultivar. The ratios by patterns were highest in entirety (98.6%) and relatively high in step (60.8%), whereas, low in boarder, star and firewood (33.6-36.4%), and relatively low in half and vein (43%). Chlorophyll content of variegated leaf was rather lower compared to normal plants and chlorophyll b tended to be higher in ratio of chlorophyll a to b. Particularly content of chlorophyll b in Stromanthe sanguinea 'Triostar', Dracaena fragrans 'Massangeana Compacta', D. reflexa 'Song of India', and Tradescantia spathacea was higher than chlorophyll a in comparison with that of normal plants.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.165-168
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• 1989

Ginseng plants in the 2nd year were grown under the shade (90-95%) of colored cellophane films at $15^{\circ}C$ in a natural light phytotron. Relative root grown (final/initial) was in decreasing order of blue, green, white, yellow and red. The growth of aerial part, especially stem length was poorest in blue light with relative root growth negatively correlated with stem length. In this relation each color showed specific domain. Stem length showed positive linear correlation with leaf area per plant in each and among light though it was not significant in red light. The content and ratio of chlorophyll a and b in leaf were in the decreasing order of White, Blue, Green, Red and Yellow. There is positive correlation between chlorophyll a and chlorophyll b, and between total chlorophyll and chl:a/b ratio. Blue color shade is expected to be beneficial for ginseng production.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.2
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• pp.173-181
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• 1993

Seasonal distributions of nutrients and particulate organic matter were investigated in Asan Bay, Korea. Most of nutrients were high in August and low in February. The atomic ratios of inorganic nitrogen to phosphorous were close to Redfield ratio except in May when the ratio was 24.8. In May, nutrient concentrations except phosphorous decreased with salinity until $31.5{\sim}32.0%0$, but the concentrations increased again with salinity, impling that there were nutrient input sources within the estuary. Howerer, significant inverse relationships between nutrients and salinity in August suggest that nutrient sources were river discharge. Maximum chlorophyll a concentrations occurred in May. Relatively low ratios of $R_b$ to $R_a$($R_b$: fluorescence before acidification; $R_a$: fluorescence after acidification) during the study periods indicate that phytoplankton were not in good physiological condition. Relatively low ratio of particulate biogenic silica(PBSi) to particulate organic carbon(POC) and high ratios of PBSi and POC to chlorophyll a during the study periods suggest input of non-living detrital PBSi and POC from bottom in Asan Bay, where strong tidal mixing occurs.