• Title/Summary/Keyword: chlorophyll ${\alpha}$

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Removal Effects of Chlorophyll-a and Cyanobacteria Using Laboratory-scale Biomanipulation Tests (실험적 생물조절 기법을 이용한 엽록소 및 남조류 제거 효과)

  • Lee, Sang-Jae;Lee, Jae-Yon;Lee, Jae-Hoon;Bae, Dae-Yeul;Lee, Eui-Hang;Han, Jung-Ho;Hwang, Soon-Jin;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.41 no.spc
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    • pp.86-92
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    • 2008
  • This study was conducted to determine removal effect on phytoplankton (chlorophyll-${\alpha}$ as whole algae) and cyanobacteria by a fish Pseudorasbora parva and macroinvertebrate Palaemon paucidens in September 2006. Three treatments with 25 (T1), 50 (T2) and 100 (T3) individuals along with control (C1, no input fish), and two treatments with 25 (T4) and 50 (T5) individuals along with control (C1) were made for fish and macroinvertebrate, respectively. The initial concentrations of chlorophyll-${\alpha}$ $(Chl_i)$ in each 10L test tank were set up for the levels of $95{\sim}100{\mu}g\;L^{-1}$ and the daily values were monitored in the test tank during 7 days. In the lab tests, P. parva did not show Chl-${alpha}$ removal effect; the removal rate of Chl-${alpha}$ for P. parva was -58% in T1, -56% in T2, and 61% in T3 during the test period. In contrast, P. paucidens. in the treatments of T4 and T5 removed the phytoplankton effectively and the removal effect were appeared to be 33% and 22%, respectively. Also, P. paucidens showed high feeding efficiency in the removal of cyanobacteria. The levels of cyanobacteria were greatly lowed from 6,048 to 927 cells $mL^{-1}$ in T4 and from 6,539 to 1,053 cells $mL^{-1}$ in T5, resulting in 85% and 84% in the removal effect, respectively. Our results for biomanipulation tests suggest that P. paucidens may be used as a potential candidate organism for algae control in spite of the preliminary results by laboratory tests.

An Application of $^{13}C$ Tracer for the Determination of Primary Productivity and Fatty Acid Production Rate in Shingu Reservoir (신구저수지의 1차 생산 및 지방산 생성속도 결정을 위한 $^{13}C$ 추적자 활용 연구)

  • Lee, Yeon-Jung;Kim, Min-Seob;Shin, Kyung-Hoon;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.41 no.spc
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    • pp.21-26
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    • 2008
  • The in situ incubation experiment was carried out using $^{13}C$ tracer to determine primary productivity and fatty acid production rate in the shallow, eutrophic Shingu reservoir on 4th July. Particulate organic matter (POM) accounted for 76% in suspended particulate matter (SPM), and average concentration of chlorophyll-${\alpha}$ was $89{\mu}g\;L^{-1}$ in the euphotic layer. Total amount of chlorophyll-${\alpha}$ (Chl-${alpha}$), primary productivity and Chl-${alpha}$ specific productivity in euphotic layer were 112 mg Chl-${alpha}m^{-2}$, 3.53 g C $m^{-2}\;d^{-1}$, 32mg C mg Chl-${\alpha}^{-1}\;d^{-1}$, respectively. The fatty acid composition in newly produced organic matter and suspended organic matter didn't show any significant difference, demonstrating that autochthonous organic matter should be a major source of POM pool. In addition, the fatty acids of bacterial origin were increased through extracellular release of newly photosynthesized DOC, and closely coupled with bacterial assimilation. This result suggests that organic carbon should be actively cycled through the microbial loop in Shingu reservoir in summer.

Nutrients and Chlorophyll Dynamics Along the Longitudinal Gradients of Daechung Reservoir (대청호에서 종적구배에 따른 영양염류 및 엽록소의 역동성)

  • Bae, Dae-Yeul;Yang, Eun-Chan;Jung, Seung-Hyun;Lee, Jae-Hoon;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.40 no.2
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    • pp.285-293
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    • 2007
  • The study was to determine zonal characteristics of nutrients and chlorophyll and evaluate their trophic relations in Daechung Reservoir. For this study, we compared longterm water quality data among three zones along with trophic state using 1993 to 2002 dataset, obtained from the Ministry of Environment, Korea. Total phosphorous (TP), Secchi depth (SD) and chlorophyll (CHL) showed typical longitudinal declines from the riverine to lacustrine zone, but total nitrogen (TN) was not evident. Largest seasonal variations in TP and CHL occurred during the summer monsoon from July to August. In the reservoir, ambient TN averaged 1.67 mg $L^{-1}$ and ratios of TN : TP averaged 88.04, indicating that nitrogen is not likely limited but phosphorus limitation was evident. Trophic State Index (TSI), based on CHL, TP, and SD, varied depending on the zones and seasons. Mean TSI (TP) in the riverine zone was 62 during the monsoon, indicating a hypertrophic condition, whereas the mean was 40 in the lacustrine, indicating a nearly oligotrophic. Values of TSI (CHL) showed maximum in the transition zone during the monsoon. The deviation analysis of TSI showed that about 65% of TSI (CHL)-TSI (TP) and TSI (CHL)-TSI (SD) values were less than zero and the lowest values were -42, indicating an effect of inorganic turbidity on algal growth in the reservoir. Correlation analysis of CHL vs. SD shewed greater correlation coefficient (p<0.001, r=-0.47) in the transition than other two zones (p<0.001, $r{\leq}-0.40$). Correlation analysis of TP vs. CHL was greatest in the lacustrine and TP was minimum in the lacustrine zone, indicating a lowest yield of algal biomass in the lacustrine. Overall data suggests that zonal response of chlorophyll yield at a given nutrient unit is clearly differed among the longitudinal gradients, so the management strategy such as cross sectional modelling should be provided in each zone.

Correlations and Seasonal Variations of Marine Viral Abundances, Bacterial Abundances and Concentration of Chlorophyll-$\alpha$ in Gwangyang Bay (광양만내 해양 바이러스에 대한 엽록소$\alpha$와 박테리아 개체량의 계절적 변이와 상관관계)

  • Choi, Eun-Seok;Kim, So-Jung;Oh, Ro-Ra;Yun, Hee-Young;Shin, Kyung-Soon;Chang, Man;Lee, Sukchan;Lee, Sang-Seob;Lee. Taek-Kyun
    • Korean Journal of Environmental Biology
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    • v.22
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    • pp.86-92
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    • 2004
  • The marine viral density in the Gwangyang Bay was abundant about 2.0${\times}$10$^{8}$ particles ml$^{-1}$ . For each season, viral abundances were recorded from 9.0${\times}$10$^{8}$ particles ml$^{-1}$ in summer to 0.7${\times}$10$^{6}$ particles ml$^{-1}$ in winter. The spatial distributions of the viral, bacterial and phytoplankton biomass in the Gwangyang Bay were mostly highey in closed estuarine system (Station 2, 5, 10, 12, 16, 20) than open ocean system (Station 28, 38, 42, 46, 51), And the othey closed estuarine system (Station 22, 26, 32, 34) indicated higher viral abundances, lower bacterial and plankton biomass than open oceanic system. In depths of some stations, the bacterial abundances exceeded a hundred fold than viral abundances. Seasonal abundances of marine viruses and their host systems were dynamically changed, and their seasonal variations were closely correlated. In summer, viral and bacterial abundances were increased, and phytoplankton chlorophyll $\alpha$ concentrations were maintained in average values. In winter, viral and bacterial abundances were dramatically decreased, and chlorophyll a concentrations were decreased, but, immediately increased. The viral abundances were peaked in August 2001, and bacteyial abundance, in August 2001 and June 2002, while chlorophyll a concentrations were peaked in April. 2002. In total host and viral abundances, it was seemed that their pools were maintained to steady-states by viral mortality, and viral abundance maintained steady-states. In our assessments, this report is a unique research about marine viral ecology of the Gwangyang Bay in Korea.

Elucidation of Anti-tumor Initiator and Promoter Derived from Seaweed-3 : Anti-tumor Promoters of Ecklonia stolonifera Extracts (해조류 중의 anti-tumor initiator 및 promoter의 해석-3 : 곰피 추출물중의 발암 promotion억제 인자)

  • PARK Young-Beom;KIM In-Soo;YOO Sung-Jae;AHN Jong-Khan;LEE Tae-Gee;PARK Douck-Chon;KIM Seon-Bong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.4
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    • pp.587-593
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    • 1998
  • To elucidate anti-tumor promoter from seaweed, the anti-tumor promoting activity of Ecklonia stolonifera, Undaria pinnatifida and Laminaria japonica extracts were determined by Epstein-Barr virus (EBV)-early antigen (EA) induction caused by a tumor promoter, teleocidin B-4. The methanol extracts of seaweed were subsequently fractionated with diethyl ether, distilled water, chloroform and ethyl acetate. Among the solvent fractions tested, chloroform and ethyl acetate fraction of E. stolonifera showed a high anti-tumor promoting activity at the levels of 88.0 and $85.9\%$ by the addition of 20 ${\mu}g/m{\ell}$, respectively. To characterize anti-tumor promoters from solvent fractions of E. stolonifera, the effects of phenols, chlorophyll derivatives and carotenoids on the anti-tumor promoting activity were investigated. Phenols, such as bromophenol and phloroglucinol showed anti-tumor promoting activity of $57\~66\%$ at 20 ${\mu}g/m{\ell}$. Pigments, such as chlorophylls and carotenoids exerted high anti-tumor promoting activities. Chlorophyll a and pheophorbide a exhibited the activity of $77.4\%$ and $66.6\%$ at 5${\mu}M/m{\ell}$, respectively. The active compounds of carotenoids were tentatively identified as lutein and $\alpha-cryptoxanthin$ from the profiles of visible spectra and R_f value of their authentic compounds, and showed anti-tumor promoting activities of $76.9\%$ and $84.4\%$ at dose of 20 ${\mu}g/m{\ell}$, respectively.

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A Methodology for Speckle-free SeaWiFS Chlorophyll-$\alpha$ in the East Sea (동해 SeaWiFS 클로로필-$\alpha$ 의 스펙클 오차를 제거한 합성장 생산)

  • Chae, Hwa-Jeong;Park, Kyung-Ae
    • Proceedings of the KSRS Conference
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    • 2009.03a
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    • pp.344-349
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    • 2009
  • 동해의 SeaWiFS 클로로필-$\alpha$에서 나타나는 스펙클(speckle) 오차를 제거하고 Level-2에서 Level-3로의 자료합성을 실시하였다. 1998년부터 2007년까지 10년간 클로로필-$\alpha$의 시공간 변동성을 고려하여, 월 평균값으로 표준화된 공간적 표준 편차가 $7mg/m^3$ 이상 될 때를 경계 값으로 스펙클 제거기준을 설정하였다. 농도경사가 큰 전선지역과 연안으로부터 10-50 km 이내 지역은 제거 기준을 $12mg/m^3$ 이상으로 알고리즘을 구성하였다. 스펙클 제거 결과, 자료는 그대로 보존되고 스펙클이 존재하는 화소의 값만 줄어들었다. 특히 스펙클이 자주 발생하는 겨울철 클로로필 자료의 농도변화가 크게 나타났으며 스펙클이 많이 나타나는 시기에는 $5^{\circ}\times5^{\circ}$영역의 평균값이 10%이상 감소하는 결과를 보였다.

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Study on the Korean Waters using the CAL/VAL of the OSMI Level 2 Data

  • Suh, Young-Sang;Jang, Lee-Hyun;Mitchell, B.G.;Kahru, M.;Prasad, Kota;Shin, H.Y.
    • Korean Journal of Remote Sensing
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    • v.18 no.3
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    • pp.127-139
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    • 2002
  • A comparison was made between the chlorophyll $\alpha$ and suspended solid (SS) retrievals from OSMI and SeaWiFS sensor to chlorophyll $\alpha$ and SS values determined with the standard method during the NFRDI's research cruises. The percentage of organic and inorganic materials from the SS was calculated to study the contribution of turbid water in the northern part of the East China Sea. The open sea waters in the Kuroshio regions of the East China Sea showed relatively higher concentration of volatile SS. However, towards the northwestern part of the East China Sea, the situation became much more optically different with the non-volatile SS from the Yangtze river and the sea bottom sources in the sea in winter and spring seasons. Furthermore, in order to indirectly detect low salinity water with high turbidity, which related to the Yangtze river using remote sensed data from the satellites, a comparison between the results of the band ratio(nLw 490nm/nLw 555nm) of SeaWiFS(OSMI) and the distribution of low salinity around the Jeju Island was presented.

Diel migration of Dinoflagellates, Cochlodinium polykrikoides in situ (Dinoflagellates, Cochlodinium polykrikoides의 현장 일부이동)

  • 나기환;박경대;이숙희;김건효;남정배
    • Journal of Aquaculture
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    • v.10 no.4
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    • pp.457-462
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    • 1997
  • During the period of the Cochlodinium polykrikoides red tide occurrence in the southern coast of Korea in 1997, we obseved the vertical migration of red tide organisms in situ, along with environmental factors such as water temperature, dissolved oxygen, pH, chlorophyll $\alpha$ and nutrients for practical application of loess suspension techniques ot diminish fisheries damage. To cope with serious outbreaks of red tide of C. polykrikoides, the finfish culturists resorted with loess suspension to disperse the bloom watermass by turning around it with fishing boats in full speed. Peak in afternoon the cell numbers were diminished in the surface layer after sunset red tide organisms settled down in the middle and bottom layer progressively again after sunrise. Chlorophyll $\alpha$ and dissolved oxygen showed the same trends of variation with the cell number. In the course of controlling the red tide with loess suspension we could not find any severs oxygen deficiency to kill the culture species.

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Top-down Fish Biomanipulation Experiments on Algal Removal Effects (조류제거 효과에 대한 Top-down 어류 조작실험)

  • Lee, Sang-Jae;Lee, Jae-Yon;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.40 no.3
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    • pp.431-438
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    • 2007
  • This study was to test algal removal efficiency by top-down fish biomanipulation experiments in the laboratory during Agust${\sim}$September 2000. We selected eight candidate fishes for the biomanipulation. We set up the experiments of eight fish-treatment tanks (3${\sim}$6 fishes) with initial chlorophyll-${\alpha}$ concentrations (CHL-${\alpha}$) of $100{\sim}120{\mu}g\;L^{-1}$ and one control tank including no fish with the same initial CHL-${\alpha}$. All tanks were maintained water quality of dissolved oxygen $(5.3{\sim}8.2mg\;L^{-1})$ and pH $(7.4{\sim}8.1)$ in the tests. During the biomanipulation, DO and pH in the treatments were lower than those of the control, while conductivity increased gradually in the treatments. Biomanipulation experiments showed that CHL-${\alpha}$ increased 13% and 0% (mean values of 8 fishes) in the controls and treatments, respectively. These results indicate that algal growth was maintained in the control and fish treatments, but the rate of CHL-${\alpha}$ in the treatments was lower than that of the control. The removal rates of bluegreens algae decreased 32% in the control, and 20% in treatments (mean values of 8 fishes) respectively, In other words, bluegreen algae showed greater growth rate in the fish treatments than the control and this was due to higher nutrients supplied from fish excretions. Overall, simple fish biomanipulation on algal control was not effective at all in these laboratory tests.