• Food Science of Animal Resources
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• v.41 no.1
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• pp.34-44
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• 2021

Buffalo animals are slaughtered at their early age and carcasses are chilled rapidly which deteriorates its meat quality and decreases the consumer likeliness of buffalo meat. This study investigated the appropriate methods to prevent the quality deterioration of buffalo meat during chilling. Twenty four 18-mon-old buffalo bulls were slaughtered, electrically stimulated and suspended either by hip or achilles tendon. After 24 h postmortem, meat quality characteristics were recorded. Results showed that electrical stimulation (ES) led to rapid decline of carcass pH compared to non-ES method (p<0.05). Furthermore, electrically stimulated meat presented lower shear force accompanied with the higher CIE L⁎, a⁎, and b⁎ values (p<0.05). Suspension methods only affect the meat shear values and were lowered in hip suspended samples. It can be concluded that ES combined with hip suspension can be adopted to prevent the meat quality deterioration of young buffalo bulls during postmortem storage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.2
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• pp.151-158
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• 1993

Since the inhibition of floral induction was considered to be an important subject to get high yield of Angelica gigas, the effects of low temperature, daylengh and shading were investigated in this study. The yield of A. gigas root grown by inhibition of floral induction was compared with the yield obtained by traditional cultivation method. When the seedings were exposed to the natural low air temperature condition until late of November, the plants which had 6 to 8 leaves showed vernalizing effect, and when the low temperature treatment was prolonged until mid of January, 4 to 5 leaf plants showed vernalizing effect. But 2 to 3 leaf plants did not show vernalizing effect regardless of the length of chilling treatment. The effectiveness of exposure to low temperature was markedly dependent upon the age of plants. In the artificial low temperature treatment the range of temperature varied with plant age. Vernalizing effect at 1,5 and 9$^{\circ}C$ of temperature did not show 3,5,6 leaf plants, respectively. The bolting percent in 8 leaf plant at 1,5 and 9$^{\circ}C$ for 8 weeks decreased by 80,45 and 5%, respectively. As daylength became longer, the bolting percent of A. gigas was increased. plants which were grown in the full sunlight showed the highest value in bolting percent, and the bolting percent was not decreased significantly with changing from full sunlight to 50% shading. When A. gigas were grown by the inhibition of floral induction, dry root weight per l0a showed the greatest value in 3 year old plant which 3.6 and 2.4 times higher than that of 1 and 2 year old plant, respectively. The yield of root was increased by 73.7% in 2 year old plant and 159.6% in 3 year old plant compared with the yield obtained by traditional cultivation method.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.4
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• pp.221-232
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• 2009

The geographical distribution of freeze risk determines the latitudinal and altitudinal limits and the maximum acreage suitable for fruit production. Any changes in its pattern can affect the policy for climate change adaptation in fruit industry. High-definition digital maps for such applications are not available yet due to uncertainty in the combined responses of temperature and dormancy depth under the future climate scenarios. We applied an empirical freeze risk index, which was derived from the combination of the dormancy depth and threshold temperature inducing freeze damage to dormant buds of 'Changhowon Hwangdo' peach trees, to the high-definition digital climate maps prepared for the current (1971-2000), the near future (2011-2040) and the far future (2071-2100) climate scenarios. According to the geospatial analysis at a landscape scale, both the safe and risky areas will be expanded in the future and some of the major peach cultivation areas may encounter difficulty in safe overwintering due to weakening cold tolerance resulting from insufficient chilling. Our test of this method for the two counties representing the major peach cultivation areas in South Korea demonstrated that the migration of risky areas could be detected at a sub-grid scale. The method presented in this study can contribute significantly to climate change adaptation planning in agriculture as a decision aids tool.

• Current Research on Agriculture and Life Sciences
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• v.1
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• pp.141-150
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• 1983

This report decribes the changes of internal browning and PPO in apples, when the method of storage was changed in the condition of low temperature. Internal browning was found more Fuji harvested in November than Fuji in October. The degree of internal browning was the highest during the storage of 0.09mm 2% and 0.06mm 2%, 0.06mm 20% was followed. In the subatmospheric storage with complete remove of $CO_2$ internal browning was not observed. During the storage of P.E. film bag, Fuji havested in November was higher than in October, and Fuji on 0.09mm 2% was higher than in 0.06mm, and Fuji during the storage of P.E. film bag was increased in PPO activity and PPO specific activity. During the subatmospheric storage with complete remove of $CO_2$, PPO activity of Fuji was little changed, but it's PPO specific activity was continuously decreased. PPO activity of Ralls was more higher than PPO activity of Fuji. PPO activity and PPO specific activity during the early stage of storage and the end stage of storage were little changed. Internal browning of Fuji was more effected $CO_2$ injury than chilling injury. There is no relation between internal browning and degree of PPO activity was effected by $CO_2$ and slightly related with internal browning.

• Korean Journal of Agricultural and Forest Meteorology
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• v.15 no.1
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• pp.40-49
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• 2013

Current service system of the Korea Meteorological Administration (KMA) for blooming date forecasting in spring depends on regression equations derived from long term observations in both temperature and phenology at a given station. This regression based system does not allow a timely correction or update of forecasts that are highly sensitive to fluctuating weather conditions. Furthermore, the system cannot afford plant responses to climate extremes which were not observed before. Most of all, this method may not be applicable to locations other than that which the regression equations were derived from. This note suggests a way to replace the location restricted regression equations with a thermal time based phenology model to complement the KMA blooming forecast system. Necessary parameters such as reference temperature, chilling requirement and heating requirement were derived from phenology data for forsythia, azaleas and Japanese cherry at 29 KMA stations for the 1951-1980 period to optimize spring phenology prediction model for each species. Best fit models for each species were used to predict blooming dates and the results were compared with the observed dates to produce a correction grid across the whole nation. The models were driven by the KMA's daily temperature data at a 5km grid spacing and subsequently adjusted by the correction grid to produce the blooming date maps. Validation with the 1971-2012 period data showed the RMSE of 2-3 days for Japanese cherry, showing a feasibility of operational service; whereas higher RMSE values were observed with forsythia and azaleas.

• Korean Journal of Food Science and Technology
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• v.35 no.6
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• pp.1007-1016
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• 2003

Garlic was stored at 4, 8, and $12^{\circ}C$ to investigate the development of green discoloration. Green discoloration developed after 7 day of storage ar $4^{\circ}C$, while it developed after 15 day of storage at 8 and $12^{\circ}C$. The effect of maleic hydrazide fertilization on green discoloration of garlic was not observed. Green discoloration of garlic was accelerated by gamma-radiation treatment. The addition of cysteine did not prevent green discoloration, which decreased the commercial value of the garlic due to the presence of white specks on the surface. When 3% ascorbic acid was added to the garlic, green discoloration developed in 6 and 24 hr at room temperature and $4^{\circ}C$, respectively. The tendency of garlic to discolor was also investigated at various storage temperatures. Discolored garlic stored for 30 day at low temperatures was conditioned at $20{\sim}45^{\circ}C$ for 20 day. The green discoloration of garlic conditioned at 20 and $25^{\circ}C$ did not disappear in 20 day, but disappeared in 20 day when conditioned at $30^{\circ}C$. The L, a, and b values of garlic conditioned at 35, 40, and $45^{\circ}C$ for 4 day were similar to those of normal garlic. Conclusively, our results indicated that the best method for suppressing green discoloration was conditioning discolored garlic at $35^{\circ}C$ for 4 day.

• Journal of Embryo Transfer
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• v.16 no.1
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• pp.35-40
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• 2001

Artificial insemination (AI) with frozen or cooled semen is widely used in commercial fields of cattle and pig. Little is known about characteristics of canine sperm after freezing or cooling. For both practical and commercial goal, the canine semen treated with cooling and freezing should be carried out to exam the fundamentals, including sperm motility, survivability and fertilizing capacity. The aim of this study, thus, was to identify the effects of extended exposure to 4$0^{\circ}C$ on canine semen by motility, survivability, acrosomal changes following different duration. Fifteen ejaculates collected by digital manipulation twice per week from 3 dogs (Shih-Tzu) were divided to 16 aliquots after adding Tris-egg yolk (TE) buffer formulated by our laboratory, and cooled from 37 to 4$^{\circ}C$, by ramp rate of 0.6$^{\circ}C$/min. Each sample was evaluated by their motility, survivability and the acrosomal status at 0h (control), 2h, 12h and 1 d~10 d, respectively. The motility of spermatozoa was graded to 6 levels using the modified method of Seager. The survivability of sperm was assessed using an epifluorescence microscope after Fert/Light (Mole-cular Probes Inc.) staining. To estimate the proportion of the spermatozoa of intact acrosome, 200 spermatozoa were assessed in randomly selected fields, using epifluorescence microscope after FITC/PSA (Sigma) staining. At 2 h after cooling, the motility of most spermatozoa were assessed to be grade 0 and 1. At 12 h, high number of sperm were in grade 0 to 1, however, it was significantly (P<0.05) lower than that of 2 h. From 1 d to 4 d, ~50% of sperm was assessed to grade 0 to 1. On day 7, a little sperm were in grade 0 to 1. No sperm showed motility on day 10. Sperm motility was rapidly reduced by the percent of 10% of grade 0 to 1. From 2 h to 6 h, the number of live sperm was 90% and the sperm chilled for 10 days lived>50%. Acrosomal intact of spermatozoa exposed to 4$^{\circ}C$ for 2 h was 51%, supposed the sperm of control was 100%. Our results suggest that 1) this is easy to transfer and preservation for short periods 2) AI can be used by semen chilled for 6-Day.

• The Journal of Internal Korean Medicine
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• v.10 no.1
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• pp.65-80
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• 1989

About Oui-Ga-Sil(胃家實) in order to considerate the contents recorded in Nai-Gyung Sang-Han-Lon and latter literature, definition, etiopathology, syndrome, differential diagnosis, therapy, Prognosis and prevention were classified. And the results were as follows: 1. Oui-Ga(胃家)was a term which indicated the whole digestive system such as stomach, small intestine, large intestine, rectum and anus. Sil(實)could be defined as the peculiar concept pertaining to the acute and last stage which was invaded to inside bowels because of abundance with evil influence. 2. Eliology of Oui-Ga-Sil was abunt gastric fever originally, injured mucus because of mistreatment, the invasion to inside of outside evil influence through meridian. Pathology was the opening and shutting appearance of gastric abundance with intestinal emptiness, and intestinal abundance with gastric emptiness, Oui-Ga-Sil could be occurred because of gastric abundant dryness and splenic humidifying capacity decrease. 3. Symptom of Oui-Ga-Sil was classified as for the sunlight outside syndrome and the inside abundant syndrome. The sunlight outside syndrome was body fever, sweating, no chilling, on the contrary hatred of fever. The chief complaint of inside abundant syndrome was daily fever, talking in delirium, hand and foot sweating, abdominal distention, difficult defection and those could be pertained to sunlight bowel syndrome. 4. Diagnostic views of Oui-Ga-Sil were that pulse was descending abundant large strong and smooth quick, a coated tongue was yellow, deep yellow, old yellow, thick, scorching dry rough or gray black. On abdominal diagnosis, pressing by hand, patient was conscious of pain, excessive pain, rejection against press, impossible press or intermittent abdominal pain and bowel cutting pain without press. 5. Differential diagnosis was that the sunlight of Nai-Gyung-Fever-Theory was outside desease making meridian the prime object, Baik-Ho-Tang syndrome was making figureless abundant fever the pivotal point. And important differential standard of splenic shrink syndrome was that a daily fever, an irritation with fever were not occurred. 6. Theory of Oui-Ga-Sil was that Seng-Gi-Tang classes had been used in attacking downward or making balance, and moxibustion on Jung-Wan, honey boiling induction theory had been also used. Attacking downward therapy was invigorating method to preserve mucus, and if mucus had been exausted with complicating emptiness prognosis had been appeared badly. 7. Preventing from Oui-Ga-Sil diet by rule, fitness to cold and warmth may be needed to prevent outside evil influence attack and inside evil influence occurrence. Prudence with being very busy, fatigue, wine and woman may be also needed not to be an injury to splanic and gastric spirit.

• Food Science of Animal Resources
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• v.20 no.2
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• pp.152-158
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• 2000

This study was conducted to investigate the influence of feeding illite(yellow soil component) on meat quality of pork. 40 pigs were fed with commercial formulated feed (control group) and 1% illite added feed(treatment group). Pigs grown up to 100~110kg of weight were slaughtered in commercial abattoir. After chilling for 24hours, samples were collected from M. longissimus dorsi muscle, and stored at $4\pm$$1^{\circ}C$ for analyses. The physico-chemical properties was determined during storage days as follows. Ultimate pH was not significantly different between control and treatment(pH 5.58 and 5.60, respectively). Water-holding capacity measured using filter paper press method of the treatment group was slightly higher than that of the control group. Cooking loss of treatment group were significantly lower than that of the control group at 1 and 7 days(p〈0.05). Shear force between control and treatment group was not different. L*- and a*-value were not significantly different between the two groups(p〉0.05), but b*-value of treatment group was lower than that of the control group(p〈0.05). The drip loss of all samples was increased with storage days, and that of the treatment group was significantly lower than that of the control at 1 and 3 day storage. Meat color measured using NPPC pork quality standards was not significantly different between the two groups(p〉0.05), but marbling content of the treatment group was significantly higher than that of the control group(p〈0.05). Sensory characteristics of, flavor taste and tenderness did not show significant differences(p〉0.05), but juiciness of the treatment group showed higher value than that of the control group(p〈0.05)

• Food Science of Animal Resources
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• v.22 no.3
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• pp.228-233
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• 2002

To investigate the effects of submersion aging in chilled water on tenderness and microbial growth of vacuum-packed beef, the semimembranosus muscles of Hanwoo (Korean cattle) was sampled at a commercial meat plant at 24 hrs postmortem. The samples were cut to 2.5 cm thick steaks and randomly assigned to following two treatments. The samples were stored in conventional refrigerator at 4$^{\circ}C$ after vacuum packaging for control whereas the other vacuum-packed samples were submersed in chilled water at 1$^{\circ}C$ for treatment, and both samples were stored for 14 days to measure total plate counts (TPC), sarcomere length, free calcium concentration, shear farce value and myofibrillar fragmentation index (MFI). The sarcomere length of treatment was significantly (p<0.05) longer than that of control at 3 days aging. Result suggested that submersion in chilled water of vacuum-packed beef might be effective to improve tenderness of meat compared to storage in conventional refrigerator. There were no significant differences in the shear force and MFI between control and treatment during storage. However, the free calcium concentration of samples from treatment was significantly (p<0.05) higher than that of control at 7 days of ageing. This result indicated that the lower shear force value and the longer sarcomere length of samples from treatment might be due to increasing the free calcium ion concentration in sarcoplasm during storage. On the other hands, samples from control showed significantly (p<0.05) higher number of microbial (TPC) compared to treatment during storage. from results obtained, submersion in chilled water of vacuum-packed beef could be recommended as a desirable aging method to improve tenderness of Hanwoo compared to aging in conventional refrigerator.