• 한국식품조리과학회지
• /
• 제32권1호
• /
• pp.58-64
• /
• 2016

본 연구는 돈육 후지 부위를 영양학적으로 우수한 머리고기로 대체한 햄버거 패티의 품질 특성을 파악하여, 경제적이면서 고품질의 햄버거 패티를 개발하고자 연구를 실시하였다. 수분 함량, 명도, 적색도, 황색도, 가열수율 및 보수력은 머리고기 첨가량이 증가함에 따라 감소하는 추세를 나타냈으나, 지방 함량 및 회분 함량은 대조구와 머리고기를 첨가한 처리구간에 유의적인 차이를 보이지 않았다. 또한 머리고기 첨가량이 증가함에 따라 단백질 함량, pH, 직경감소율, 두께감소율 및 전단력은 증가하는 경향을 나타내었다. 관능적 특성도 색 및 풍미에서 대조구와 비교하여 15%까지 머리고기를 첨가한 처리구(T1, T2, T3)에서는 유의적인 차이가 인정되지 않았으며, 전체적인 기호도에서는 대조구와 비교하여 머리고기를 10%까지 첨가한 처리구에서 유의차가 나타나지 않았다. 따라서, 활용도가 낮은 돈육 부산물 중 머리고기를 활용하여 햄버거 패티를 제조할 시 머리고기의 첨가량이 10% 이하로 첨가하는 것이 햄버거 패티의 품질 및 관능적으로 우수한 식육제품을 제조할 수 있을 것으로 보여진다.

• The Korean Journal of Ecology
• /
• 제22권5호
• /
• pp.235-240
• /
• 1999

토지이용이 어떻게 수질에 영향을 주는가를 연구하고자 1993년 3월 부터 1998년 3월 까지 조사를 실시하였다. 조사지는 충북 옥천군 이원면의 경작지가 주류를 이루는 개심저수지와 산지유역으로 특성지워지는 장찬저수지 유역을 대상으로 하였다. 조사유역을 11개 소집수역으로 세분하고 토지이용과 하천수질과의 상관성, 하천 유역의 오염원 동태를 생태학적 관점에서 규명하고자 조사를 실시하였다. BOD, SS, TKN은 하류로 갈수록 높아졌으나 자정 한계를 넘어선 G 소집수역은 예외였다. 장찬저수지 유역은 가두리 양식장 때문에 수질이 악화되어 있는 것으로 나타나 양식장의 폐쇄가 시급한 것으로 조사되었다. .해발표고는 200 m 이하가 개심저수지 유역 56.0%, 장찬저수지 유역 44.0%로 대부분을 차지하였다. 비삼림지역은 전체 조사면적 44.91 ㎢중 14.74 ㎢로 32.8%를 차지하였는데 특히 경작지와 주거지 면적 비의 증가에 따라 총인(Y=0.2023X＋0.0991, r=0.54)이 증가되고 있다. 토지이용별 오염 배출원 단위가 수질에 미치는 영향을 분석한 결과 농경지의 오염부하량은 매우 크며 비점오염물질이 하천에 유입, 유하 하는 동안 물리, 화학, 생화학적, 생물학적 변화를 거쳐 농도가 낮아지고 있다. 그러나 삼림지역의 오염부하량은 농경지의 오염부하량 보다는 상대적으로 작으나 오히려 하천으로 유입, 유하하는 동안 오염 물질의 양이 높아졌다.

• KSBB Journal
• /
• 제21권4호
• /
• pp.312-316
• /
• 2006

본 연구에서는 은행잎 추출물의 Staphylococcus aureus에 대한 항균효과를 검증하기 위해 먼저 에탄올 농도별로 bilobalide와 ginkgolide A, B의 성분을 분석한 결과 40% 에탄올 용매를 최적 활성농도로 결정하였다. Disc diffusion test, Optical density test을 통한 S. aureus 항균실험 결과 에탄올 추출농도가 증가할수록 항균효과가 증가하나 40% 에탄올 이상에서는 항균활성에 큰 차이가 없었다. 주사전자현미경을 통하여 은행잎 에탄올 40% 추출물 16배 농축액을 처리한 균의 세포표면을 확인한 결과 심하게 손상되었음을 확인할 수 있었다. 투과전자현미경을 이용하여 관찰한 결과 은행잎 추출물로 처리한 균주에서는 세포벽이 관찰되지 않았으며, 이는 은행잎 추출물의 주성분인 bilobalide와 ginkgolide A, B가 세포벽 합성을 저해하는 것으로 보여진다.

• 한국전기전자재료학회논문지
• /
• 제28권8호
• /
• pp.531-538
• /
• 2015

This study examined the size and shape of the nano-silver particle through the analysis of electrical resistance when synthesizing nano-sized silver by using the chemical liquid reduction. Changes in particle behaviors formed according to the changes in electronic characteristics by electric resistance in each time period in the beginning of reduction reaction in a course of synthesizing the nano-silver particle formation were studied. In addition, analysis was conducted on particle behaviors according to the changes in concentration of $AgNO_3$ and in temperature at the time of reduction and nucleation and growth course when synthesizing the particles based on the particle behaviors were also examined. As the concentration of $AgNO_3$ increased, the same amount of resistance of approximately $5{\Omega}$ was increased in terms of initial electronic resistance. Furthermore, according to the result of formation of nuclear growth graph and estimation of slope based on estimated resistance, slops of $6.25{\times}10^{-3}$, $2.89{\times}10^{-3}$, and $1.85{\times}10^{-3}$ were derived from the concentrations of 0.01 M, 0.05 M, and 0.1 M, respectively. As the concentration of $AgNO_3$ increased, the more it was dominantly influenced by the nuclear growth areas in the initial phase of reduction leading to increase the size and cohesion of particles. At the time of reduction of nano-silver particle, the increases of initial resistance were $4{\Omega}$, $4.2{\Omega}$, $5{\Omega}$, and $5.3{\Omega}$, respectively as the temperature increased. As the temperature was increased into $23^{\circ}C$, $40^{\circ}C$, $60^{\circ}C$, and $80^{\circ}C$, slopes were formed as $4.54{\times}10^{-3}$, $4.65{\times}10^{-3}$, $5.13{\times}10^{-3}$, and $5.42{\times}10^{-3}$ respectively. As the temperature increased, the particles became minute due to the increase of nuclear growth area in the particle in initial period of reduction.

• 대한치과보철학회지
• /
• 제37권6호
• /
• pp.741-755
• /
• 1999

As a material of metal-ceramic prosthesis, nickel as a form of Ni-Cr alloy has been used for many dental prostheses in many cases. However, several problems in use of the alloy have been revealed (ex : tissue stimulation, skin allergy, hypersensitivity, cytotoxicity and carcinogenecity). Little is known about nickel with respect to the relationship between Ni-prosthesis and gaining of Ni-resistance in oral microorganisms. The present study was undertaken to check wheather use of Ni-prosthesis leads to occurrence of Ni-resistant microorganisms. So this study may suggest the possible relationships between the oral microorganisms and nickel-resistance in oral environment. Bacteria were isolated from the gingival crevicular fluid on the pateints wearing Ni-Cr prosthesis. The isolated bacteria were tested for their Ni-resistance in nickel containing media at different concentration from 3mM to 110mM. E. coli HB101 was used as control. The Ni-resistant bacteria were isolated and biochemically identified. The Ni-resistant bacteria were tested several bio-chemical, molecular-biological tests. Performed tests were ; measuring the growth curve, antibiotic test, growth ability test in liquid media, isolation of the chromosome and plasmid, digestion of DNA by restriction enzyme, electrophoresis of chromosome and plasmid DNA, identification of Ni-resistant genes by the DNA hybridization. The results were as follows: 1) The bacteria isolated from gingival crevicular fluid on the patients wearing Ni-Cr alloy pros-thesis showed nickel-resistance. 2) The isolated microorganisms grew at nickel containing media of high concentrations (60mM-110mM). 3) Based on the biochemical tests, the isolated microorganisms were identified as Enterococcus faecalis(13 cases), Klebsiella pneumoniae(1 case) and Enterobacter gergeviae(1 case). 4) Enterococcus faecalis expressed not only nickel resistance but also the multi-drug resistance to several antibiotics ; chloramphenicol, kanamicin, streptomycin, lincomycin, clindamycin. However, all strain showed the sensitivity against the tetracycline. 5) DNA hybridization result suggest that there is no homology between the previousely known gene of nickel resistance in Klebsiella pneumoniae and chromosomal DNA of Enterococcus faecalis.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권13호
• /
• pp.5311-5316
• /
• 2014

Nuclear factor erythroid 2-related factor 2 (Nrf2) has been recognized as a transcription factor that controls mechanisms of cellular defense response by regulation of three classes of genes, including endogenous antioxidants, phase II detoxifying enzymes and transporters. Previous studies have revealed roles of Nrf2 in resistance to chemotherapeutic agents and high level expression of Nrf2 has been found in many types of cancer. At physiological concentrations, luteolin as a flavonoid compound can inhibit Nrf2 and sensitize cancer cells to chemotherapeutic agents. We reported luteolin loaded in phytosomes as an advanced nanoparticle carrier sensitized MDA-MB 231 cells to doxorubicin. In this study, we prepared nano phytosomes of luteolin to enhance the bioavailability of luteolin and improve passive targeting in breast cancer cells. Our results showed that cotreatment of cells with nano particles containing luteolin and doxorubicin resulted in the highest percentage cell death in MDA-MB 231cells (p<0.05). Furthermore, luteolin-loaded nanoparticles reduced Nrf2 gene expression at the mRNA level in cells to a greater extent than luteolin alone (p<0.05). Similarly, expression of downstream genes for Nrf2 including Ho1 and MDR1 were reduced significantly (p<0.05). Inhibition of Nrf-2 expression caused a marked increase in cancer cell death (p<0.05). Taken together, these results suggest that phytosome technology can improve the efficacy of chemotherapy by overcoming resistance and enhancing permeability of cancer cells to chemical agents and may thus be considered as a potential delivery system to improve therapeutic protocols for cancer patients.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권6호
• /
• pp.816-824
• /
• 2006

This study was conducted to evaluate the shoot height at which the yield and nutritive value of wormwood (Artemisia montana) is optimized in order to provide information on its potential to support animal production (Experiment 1). A second objective was to determine how the essential oil (EO) concentration in wormwood hay and silage differ (Experiment 2). In Experiment 1, Artemisia montana was harvested at five different shoot heights (20, 40, 60, 80 and 100 cm) from triplicate $1.8{\times}1.8m$ plots. Dry matter (DM) yield was measured at each harvest date and the harvested wormwood was botanically separated into leaf, stalk and whole plant fractions and analyzed for chemical composition and in vitro dry matter digestibility (DMD). Values for total digestible nutrients (TDN), digestible energy (DE) and metabolizable energy (ME) were subsequently calculated using prediction equations. Dry matter yields of stalk and whole plant increased linearly (p<0.001) and leaf yield increased quadratically (p<0.01) with shoot height, whereas the leaf/stalk ratio decreased linearly (p<0.001). As shoot height increased, there was a linear increase (p<0.001) in leaf DM, ether extract (EE) and neutral detergent fiber (NDF) contents and a quadratic increase (p<0.05) in leaf acid detergent fiber (ADF) and nitrogen free extract (NFE) contents, and stalk and whole plant DM (p<0.001), organic matter (OM, p<0.01 and p<0.05), NDF (p<0.001 and 0.05) and NFE (p<0.05) contents. However, there were decreases in leaf crude protein content (CP, quadratic, p<0.001) and stalk and whole plant EE content (linear, p<0.001), CP (quadratic, p<0.05) and ash (quadratic, p<0.05) contents. Digestibility of DM and TDN, and DE and ME value in leaves were not affected by increasing shoot height, but these measures linearly decreased (p<0.001) in stalk and whole plant. In Experiment 2, the hay had higher DM and CP concentrations, but lower EE concentration than the silage. Essential oil (EO) content in wormwood silage (0.49 g/100 g DM) was higher (p<0.05) than that in wormwood hay (0.32 g/100 g DM). Wormwood hay contained 25 essentail oils (EO) including camphor (10.4 g/100 g), 1-borneol (11.6 g/100 g) and caryophyllene oxide (27.7 g/100 g), and wormwood silage had 26 EO constituents including 3-cyclohexen-1-ol (8.1 g/100 g), trans-caryophyllene (8.6 g/100 g) and ${\gamma}$-selinene (16.8 g/100 g). It is concluded that the most ideal shoot height for harvesting wormwood is 60 cm based on the optimization of DM yield and nutritive value. Wormwood silage had a greater quantity and array of EO than wormwood hay.

• 대기
• /
• 제27권1호
• /
• pp.29-40
• /
• 2017

The $PM_{10}$ and $PM_{2.5}$ particles over the Yellow Sea of Korea were collected by ship-borne observation during two cruises in spring, 2015. Their water-soluble ionic components such as $NH_4^+$, $Na^+$, $K^+$, $Mg^{2+}$, $Ca^{2+}$, $SO_4^{2-}$, $NO_3^-$, $Cl^-$, $F^-$, $CH_3COO^-$, $HCOO^-$, and $CH_3SO_3^-$ were analyzed, in order to examine the pollution characteristics of the secondary aerosol components. The comparative study of particle size distribution has resulted that $NH_4^+$, $nss-SO_4^{2-}$, $nss-Mg2+$, $nss-K^+$, $HCOO^-$, and $CH_3SO_3^-$ species mostly existed in fine particle mode. Meanwhile, nss-F-and sea-salt species were distributed in both fine and coarse particle mode, $NO_3^-$, $nss-Ca^{2+}$, $CH_3COO^-$ species were rich in coarse particle mode. The concentrations of secondary pollutants($nss-SO_4^{2-}$, $NO_3^-$, $NH_4^+$) increased in fine particles, and those of natural components ($nss-Ca^{2+}$, Sea-salt) increased in coarse particles. $NH_4^+$ exists as the form of $(NH_4)_2SO_4$ and $NH_4NO_3$, and mostly as $(NH_4)_2SO_4$ in fine particles. $NH_4NO_3$ has lower content compared to $(NH_4)_2SO_4$, and it mostly existed in fine particles at Yellow Sea I and in coarse particles at Yellow Sea II. The concentration ratios of $NO_3^-/nss-SO_4^{2-}$ for Yellow Sea I and Yellow Sea II were 0.52 and 0.16 in coarse particles, and they were 0.64 and 0.38 in fine particles, respectively, showing that the stationary source emissions were more important than mobile source emissions in Yellow Sea II (except Passage II-4).

• 한국산림과학회지
• /
• 제95권4호
• /
• pp.423-428
• /
• 2006

녹화식물의 조기녹화와 자연식생으로의 천이를 빠르게 유도하기 위하여 임도 비탈면에 미생물제재의 사용을 통한 산림토양의 개선효과 및 녹화식물의 초기생육 상태를 분석한 결과는 다음과 같다. 토양의 pH는 미생물 처리구에서 5.69로 많이 중화되어 약산성을 보였고, 치환성 양이온도 대조구에서의 1.59(me/kg)보다 처리구에서 비교적 높은 수치인 5.04(me/kg)로 나타났다. 전질소함량(T-N) 및 유기물 함량도 처리구에서 훨씬 높게 나타나 산림토양의 비옥도가 아주 높게 개선되었음을 알 수 있었다. 미생물제재로 처리된 실험구의 평균 피복율은 대조구보다 2.5배 높게 나타났으며, 각 소형방형구의 발아 평균본수는 미생물 처리구가 대조구보다 2.7배나 높은 발생율을 보였다. 처리구별 목본인 참싸리의 초기 평균 생장량의 차이는 없는 것으로 나타났으며 참싸리의 초장은 발아 초기에 빠른 신장을 보이므로 외래초종과 혼파하면 사면안정 및 경관보전에 유리함을 알 수 있었다. 따라서 현재 외래종 위주의 단순한 종자뿜어붙이기공법의 녹화개념에서 초본 및 목본식물을 혼용한 외래종과 재래종의 사용과 미생물제재를 이용한 미생물 녹화공법의 도입은 산림토양의 개선과 사면안정 및 경관보전 효과를 높이면서 임도 비탈면의 조기녹화에 크게 기여할 수 있을 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권1호
• /
• pp.138-148
• /
• 2018

Objective: Fusarium mycotoxins deoxynivalenol (DON) and zearalenone (ZEN), common contaminants in the feed of farm animals, cause immune function impairment and organ inflammation. Consequently, the main objective of this study was to elucidate DON and ZEN effects on the mRNA expression of pro-inflammatory cytokines and other immune related genes in the kidneys of piglets. Methods: Fifteen 6-week-old piglets were randomly assigned to three dietary treatments for 4 weeks: control diet, and diets contaminated with either 8 mg DON/kg feed or 0.8 mg ZEN/kg feed. Kidney samples were collected after treatment, and RNA-seq was used to investigate the effects on immune-related genes and gene networks. Results: A total of 186 differentially expressed genes (DEGs) were screened (120 upregulated and 66 downregulated). Gene ontology analysis revealed that the immune response, and cellular and metabolic processes were significantly controlled by these DEGs. The inflammatory stimulation might be an effect of the following enriched Kyoto encyclopedia of genes and genomes pathway analysis found related to immune and disease responses: cytokine-cytokine receptor interaction, chemokine signaling pathway, toll-like receptor signaling pathway, systemic lupus erythematosus (SLE), tuberculosis, Epstein-Barr virus infection, and chemical carcinogenesis. The effects of DON and ZEN on genome-wide expression were assessed, and it was found that the DEGs associated with inflammatory cytokines (interleukin 10 receptor, beta, chemokine [C-X-C motif] ligand 9, CXCL10, chemokine [C-C motif] ligand 4), proliferation (insulin like growth factor binding protein 4, IgG heavy chain, receptor-type tyrosine-protein phosphatase C, cytochrome P450 1A1, ATP-binding cassette sub-family 8), and other immune response networks (lysozyme, complement component 4 binding protein alpha, oligoadenylate synthetase 2, signaling lymphocytic activation molecule-9, ${\alpha}$-aminoadipic semialdehyde dehydrogenase, Ig lambda chain c region, pyruvate dehydrogenase kinase, isozyme 4, carboxylesterase 1), were suppressed by DON and ZEN. Conclusion: In summary, our results indicate that high concentrations of DON and ZEN suppress the inflammatory response in kidneys, leading to potential effects on immune homeostasis.