ATP in quantity co-stored with neurotransmitters in the secretory vesicles of neurons, by being co-released with the neurotransmitters, takes an important role to modulate the stimulus-secretion response of neurotransmitters. Here, in this study, the modulatory effect of ATP was studied in $Ca^{2+}$ channels of cultured rat adrenal chromaffin cells to investigate the physiological role of ATP in neurons. The $Ca^{2+}$ channel current was recorded in a whole-cell patch clamp configuration, which was modulated by ATP. In 10 mM $Ba^{2+}$ bath solution, ATP treatment (0.1 mM) decreased the $Ba^{2+}$ current by an average of $36{\pm}6%$ (n=8), showing a dose-dependency within the range of $10^{-4}{\sim}10^{-1}mM$. The current was recovered by ATP washout, demonstrating its reversible pattern. This current blockade effect of ATP was disinhibited by a large prepulse up to +80 mV, since the $Ba^{2+}$ current increment was larger when treated with ATP ($37{\pm}5%$, n=11) compared to the control ($25{\pm}3%$, n=12, without ATP). The $Ba^{2+}$ current was recorded with $GTP{\gamma}S$, the non-hydrolyzable GTP analogue, to determine if the blocking effect of ATP was mediated by G-protein. The $Ba^{2+}$ current decreased down to 45% of control with $GTP{\gamma}S$. With a large prepulse (+80 mV), the current increment was $34{\pm}4%$ (n=19), which $25{\pm}3%$ (n=12) under control condition (without $GTP{\gamma}S$). The $Ba^{2+}$ current waveform was well fitted to a single-exponential curve for the control, while a double-exponential curve best fitted the current signal with ATP or $GTP{\gamma}S$. In other words, a slow activation component appeared with ATP or $GTP{\gamma}S$, which suggested that both ATP and $GTP{\gamma}S$ caused slower activation of $Ca^{2+}$ channels via the same mechanism. The results suggest that ATP may block the $Ca^{2+}$ channels by G-protein and this $Ca^{2+}$ channel blocking effect of ATP is important in autocrine (or paracrine) inhibition of adrenaline secretion in chromaffin cell.
Streamflow discharge as a fundamental riverine quantity plays a crucial role in water resources management, thereby requiring accurate in-situ measurement. Recent advances in instrumentations for the streamflow discharge measurement has complemented or substituted classical devices and methods. Among various potential methods, surface current meter using microwave has increasingly begun to be applied not only for flood but also normal flow discharge measurement, remotely and safely enabling practitioners to measure flow velocity postulating indirect contact. With minimized field preparedness, this method facilitated and eased flood discharge measurement in the difficult in-situ conditions such as extreme flood in active ways emitting 24.125 GHz microwave without relying on natural lights. In South Korea, a rectangular shaped instrument named with Microwave Water Surface Current Meter (MWSCM) has been developed and commercially released around 2010, in which domestic agencies charging on streamflow observation shed lights on this approach regarding it as a potential substitute. Considering this brand-new device highlighted for efficient flow measurement, however, there has been few noticeable efforts in systematic and comprehensive evaluation of its performance in various measurement and riverine conditions that lead to lack in imminent and widely spreading usages in practices. This study attempted to evaluate the MWSCM in terms of instrumen's monitoring configuration particularly regarding tilt and yaw angle. In the middle of pointing the measurement spot in a given cross-section, the observation campaign inevitably poses accuracy issues related with different tilt and yaw angles of the instrument, which can be a conventionally major source of errors for this type of instrument. Focusing on the perspective of instrument configuration, the instrument was tested in a controlled outdoor river channel located in KICT River Experiment Center with a fixed flow condition of around 1 m/s flow speed with steady flow supply, 6 m of channel width, and less than 1 m of shallow flow depth, where the detailed velocity measurements with SonTek micro-ADV was used for validation. As results, less than 15 degree in tilting angle generated much higher deviation, and higher yawing angle proportionally increased coefficient of variance. Yaw angles affected accuracy in terms of measurement area.
Journal of the Korean Society of Fisheries and Ocean Technology
/
v.24
no.2
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pp.55-64
/
1988
Stow nets have widely been used in the western sea of Korea from the olden age. The original structure of a stow net is a large square-sectional bag net made of 4 netting panels, and the front fringes of top and bottom panels are connected to the top and bottom beams respectively. Wire ropes, which is originated from the holding anchor are gradually forked and biforked, and finally 4 pieces of wire rope (biforked pendants) are jointed to each beam. Much convenience caused by long and heavy beams were problemed, then some studies have been carried out to improve the net since 1930's. The most effective improvement were achieved in 1980 by Mr. Han and his colleagues. The key point of improvement was that the beams were removed and the belt shaped shearing device made by canvas was attached to the side panels, the head rope and ground rope to the front fringe of top and bottom panel, and biforked pendants are joined to the shearing device. Even though this is the epoch-making improvement of a stow net, the further study should be required to find out more effective method. The authors carried out a model experiment on the stow net to determine the vertical and horizontal opening of a net mouth, and also examine the front, top and side-view configuration of the net. The model net was constructed depending on the Similarity Law of Fishing Gear in 1/10 and 1/20 scale and set against to the current at shallow and speedy flowing channel. The vertical and horizontal openings were determined by using scaled bamboo poles, and the configuration was observed by using specially prepared observation platform and underwater observation glass, and also photographed by using specially prepared underwater photographic equipment. The results obtained can be summarized as follows: 1. The opening height and width of the shearing device varied in accordance with the relative length of the biforked pendants. Considering the height and width of shearing device in 6 cases of the arrangement system of biforked pendants, the best result was obtained in the case that the 2nd, 3rd and 4th pendents from the bottom-most was 5%, 9% and 4% longer than that. 2. On the top-view configuration the excessive deformation of head rope and ground rope were observed. In the actual net, 54m long head rope and ground rope were attached to the front fringe of top and bottom panels so that the head rope may be lifted to make the net mouth open highly. But actually the head rope and the ground rope are streamed backward without any lift, and also the netting followed the ropes were deformed until the 2/5 in the whole length of the net. This deformation may be guessed to disturb the entrance of fish school into the net and also caused the net to get caught by obstacles in the sea bed and to be broken largely. 3. Hydrodynamic resistance R of the actual net may be deduced as R(kg)=29.2$\times$103 v1.65. It is also expressed as R(kg)=5.9$\times$d/l$\times$ab v1.65. depending on the formula deduced by Koyama to estimate the resistance of trawl nets, where d/l denote the ratio between diameter of netting twine and length of mesh leg in every part of side panel, a and b, the stretched circumference of the mouth and the stretched length of the net, respectively.
Journal of the Institute of Electronics Engineers of Korea SD
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v.49
no.8
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pp.22-26
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2012
This paper introduces a 2.5-Gb/s optical receiver implemented in a standard 1P4M 0.18um CMOS technology for the applications of active optical HDMI cables. The optical receiver consists of a differential transimpedance amplifier(TIA), a five-stage differential limiting amplifier(LA), and an output buffer. The TIA exploits the inverter input configuration with a resistive feedback for low noise and power consumption. It is cascaded by an additional differential amplifier and a DC-balanced buffer to facilitate the following LA design. The LA consists of five gain cells, an output buffer, and an offset cancellation circuit. The proposed optical receiver demonstrates $91dB{\Omega}$ transimpedance gain, 1.55 GHz bandwidth even with the large photodiode capacitance of 320 fF, 16 pA/sqrt(Hz) average noise current spectral density within the bandwidth (corresponding to the optical sensitivity of -21.6 dBm for $10^{-12}$ BER), and 40 mW power dissipation from a single 1.8-V supply. Test chips occupy the area of $1.35{\times}2.46mm^2$ including pads. The optically measured eye-diagrams confirms wide and clear eye-openings for 2.5-Gb/s operations.
In 1995 the VSOP satellite, which is called MUSES-B in Japan, will be launched under the VLBI Space Observatory Programme(VSOP) promoted by ISAS(Institute of Space and Astronautical Science) of Japan. We are now developing the GPS Receiver(GPSR) and On-board Orbit Determination System. This paper describes the GPS(Global Positioning System), VSOP, GPSR(GPS Receiver system) configuration and the results of the GPS system analysis. The GPSR consists of three GPS antennas and 5 channel receiver package. In the receiver package, there are two 16 bits microprocessing units. The power consumption is 25 Watts in average and the weight is 8.5 kg. Three GPS antennas on board enable GPSR to receive GPS signals from any NAVSTARs(GPS satellites) which are visible. NAVSATR's visibility is described as follows. The VSOP satellite flies from 1, 000 km to 20, 000 km in height on the elliptical orbit around the earth. On the other hand, the orbit of NAVSTARs are nearly circular and about 20, 000 km in height. GPSR can't receive the GPS signals near the apogee, because NAVSTARs transmit the GPS signals through the NAVSTAR's narrow beam antennas directed toward the earth. However near the perigee, GPSR can receive from 12 to 15 GPS signals. More than 4 GPS signals can be received for 40 minutes, which are related to GDOP(Geometric Dillusion Of Precision of selected NAVSTARs). Because there are a lot of visible NAVSTARs, GDOP is small near the perigee. This is a favorqble condition for GPSR. Orbit determination system onboard VSOP satellite consists of a Kalman filter and a precise orbit propagator. Near the perigee, the Kalman filter can eliminate the orbit propagation error using the observed data by GPSR. Except a perigee, precise onboard orbit propagator propagates the orbit, taking into account accelerations such as gravities of the earth, the sun, the moon, and other acceleration caused by the solar pressure. But there remain some amount of calculation and integration errors. When VSOP satellite returns to the perigee, the Kalman filter eliminates the error of the orbit determined by the propagator. After the error is eliminated, VSOP satellite flies out towards an apogee again. The analysis of the orbit determination is performed by the covariance analysis method. Number of the states of the onboard filter is 8. As for a true model, we assume that it is based on the actual error dynamics that include the Selective Availability of GPS called 'SA', having 17 states. Analytical results for position and velocity are tabulated and illustrated, in the sequel. These show that the position and the velocity error are about 40 m and 0.008 m/sec at the perigee, and are about 110 m and 0.012 m/sec at the apogee, respectively.
A V-shaped riffle is an artificial hydraulic structure haying two wings from the streamside with a narrow opening in between. It is usually made of crushed stones or large boulders. It limits channel width and accelerates the flow through the constricted section causing a local scour just downstream. The V-shaped riffle provides with a unique aquatic habitat by forming a pool and sandbars around the pool edge, increasing local morphologic, hydraulic and sedimentological diversity. This study investigates experimentally the scour characteristics of the V-shaped riffle in the sandbed stream and proposes a predictive equation for the scour. Total 45 cases of experiments were conducted to examine the effect of hydraulic factors and configuration of V-shaped riffle on the geometry of scour holes. From the comparison of the experimental results of this study with the predictive equation of spur dike by Breusers and Raudkivi(1991), it is found that their predictive equation of spur dike underestimates the maximum scour depth downstream of the V-shaped riffle. h new predictive equation for the maximum scour depth was developed using the non-dimensional hydraulic and geometrical variables. The parameters used in the proposed equations were determined using the experimental data. The analysis reveals that the scour depth is dependent dominantly on the Froude number at the opening of the V-shaped riffle, while the angle of riffle and the opening width also affect the scour depth. The proposed equation for the scour of V-shaped riffle well agrees with the experimental data. It can be used for estimating the scour of the V-shaped riffle in sandbed streams.
Transactions of the Korean Society of Mechanical Engineers
/
v.16
no.11
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pp.2150-2158
/
1992
This study was carried out to investigate the flow structure and mixing process of a cross mixing flow formed by two round jets with respect to the ratio of mass flow rate. This flow configuration is of great practical relevance in a variety of combustion systems, and the flow behaviour of a cross jet defends critically on the ratio of mass flow rate and the cross angle. The mass flow rate ratios of two different jets were controlled as 1.0, 0.8, 0.6, and 0.4, and the crossing angle of two round jets was fixed at 45 degree. The velocities issuing from jet nozzle with an exit diameter of 20mm were adjusted to 40m/s, 32m/s, 24m/s, and 16m/s, and the measurements have been conducted in the streamwise range of $1.1X_0$to $2.5X_0$ by an on-line measurement system consisted of a constant temperature type two channel hot-wire anemometry connected to a computer analyzing system. The original air flow was generated by a subsonic wind tunnel with reliable stabilities and uniform flows in the test section. For the analysis of the cross mixing flow structure in the downstream region after the cross point, the mean velocity profiles, the resultant velocity contours, and the three-dimensional profiles depending upon the mass flow rate ratio have been concentrately studied.
The Journal of Korean Institute of Communications and Information Sciences
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v.34
no.11C
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pp.1085-1097
/
2009
We present a structure and design method of the D8PSK modem compatible with the VDL mode-2 standard and performance test results of the developed modem. In VDL mode-2, the raised cosine filter is used only in the transmitter and a general low pass filter is used in the receiver. Consequently, we can not achieve ISI reduction but can have better spectrum characteristics. Although there is 1~2 dB performance degradation with an un-matched filter compared to that with a matched filter, it is more important to minimize adjacent channel interference in narrow band communications. The transmit signal is generated digitally to avoid the problems(I/Q imbalance and DC offset etc.) of analog modulators. In addition the digital down converter using digital IF sampling technique is adopted for the receiver. This paper contains the overall configuration, design method and simulation results based in part on the previously proposed structures and algorithms. It is confirmed that the modem transmits and receives messages successfully at a speed of max. 870 km/h over ranges of up to 310 km through the ground and in-flight communication tests.
Flooding hazard caused by natural and artificial environmental changes is closely associated with change in river bed configuration. This study is aimed at explaining a river-bed change related to soil erosion in the Keum river basin using GIS and RS. The USLE was used to compute soil erosion rate on the basis of GIS. River-bed profiles stretching from Kongju to Ippo were measured to construct a 3D-geomorphological map. The river-bed change was also detected by remote sensing images using Landsat TM during the period of 1982 to 2000 for the Keum river. The result shows that USLE indicates a mean soil erosion rate of $1.8\;kg/m^2/year$, and a net increase of a river-bed change at a rate of $+5\;cm/m^2$/year in the Kangkyeong area. The change in river-bed is interpreted to have been caused by soil erosion in the downstream of the Keum river basin. In addition river-bed change mainly occurred on the downstream of the confluence where tributaries and the main channel meet. Other possible river-bed change is caused by a removal of fluvial sand aggregates, which might have resulted in a net decrease of exposed area of sediment distribution between 1991 and 1995, while a construction of underwater structures, including a bridge, a reclamation of sand bars for rice fields and dikes, resulted in an increase of the exposed area of river-bed due to sediment accumulation.
Amyotrophic lateral sclerosis (ALS) is a degenerative neuromuscular disease of unknown etiology in which the upper and lower motor neurons are progressively destroyed. Recent evidences support the role of autoimmune mechanisms in the pathogenesis of ALS. This study investigated the effects of sera from ALS patients on neuromuscular transmission in phrenic nerve-hemidiaphragm preparations and on calcium currents of single isolated dorsal root ganglion (DRG) cells in mice. Mice were injected with either control sera from healthy adults or ALS sera from 18 patients with ALS of sporadic form, for three days. Miniature end plate potential (MEPP) and nerve-evoked end plate potential (EPP) were measured using intracellular recording technique and the quantal content was determined. Single isolated DRG cells were voltage-clamped with the whole-cell configuration and membrane currents were recorded. Sera from 14 of 18 ALS patients caused a significant increase in MEPP frequency in normal Ringer's solution $(4.62{\pm}0.14\;Hz)$ compared with the control $(2.18{\pm}0.15\;Hz).$ In a high $Mg^{2+}/low\;Ca^{2+}$ solution, sera from 13 of 18 ALS patients caused a significant increase in MEPP frequency, from $2.18{\pm}0.31$ Hz to $6.09{\pm}0.38$ Hz. Sera from 11 of 18 patients produced a significant increase of nerve-evoked EPP amplitude, from $0.92{\pm}0.05$ mV to $1.30{\pm}0.04$ mV, while the other seven ALS sera did not alter EPP amplitude. In the ALS group, EPP quantal content was also elevated by the sera of 14 patients (from $1.49{\pm}0.07$ to $2.35{\pm}0.07).$ MEPP frequency and amplitude in wobbler mouse were $4.03{\pm}0.53$ Hz and $1.37{\pm}0.18$ mV, respectively, which were significantly higher than those of wobbler controls (wobblers without the symptoms of wobbler). Sera from ALS patients significantly reduced HVA calcium currents of DRG cells to 42.7% at -10 mV. Furthermore, the inactivation curve shifted to more negative potentials with its half-inactivation potential changed by 6.98 mV. There were, however, significant changes neither in the reversal potential of $I_{Ca}$ nor in the I-V curve. From these results it was concluded that: 1) The serum factors of sporadic ALS patients increase neuromuscular transmission and can alter motor nerve terminal presynaptic function. This suggests that ALS serum factors may play an important role in the early stage of ALS, and 2) Calcium currents in DRG cells were reduced and rapidly inactivated by ALS sera, suggesting that in these cells, ALS serum factors may exert interaction with the calcium channel.
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