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Heterologous Expression of Human Ferritin H-chain and L-chain Genes in Saccharomyces cerevisiae (재조합 효모를 이용한 사람 H-Chain 교 L-Chain Ferritin의 생산)

  • 서향임;전은순;정윤조;김경숙
    • KSBB Journal
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    • v.17 no.2
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    • pp.162-168
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    • 2002
  • Human ferritin H- and L-chain genes(hfH and hfL) were cloned into the yeast shuttle vector YEp352 with various promoters, and the vectors constructed were used to transform Saccharomyces cerevisiae 2805. Three different promoters fused to hfH and hfL were used: galactokinase 1 (GAL1) promoter, glyceraldehyde-3-phosphate dehydrogenase(GPD) promoter and alcohol dehydrogenase 1(ADH1 ) promoter. SDS-polyacrylamide gel electrophoresis and Western blotting analyses displayed expression of the introduced hfH and hfL. In the production of both ferritin H and L subunits GAL1 promoter was more effective than GPD promoter or ADH1 promoter. Ferritin H and L subunits produced in S. cerevisiae were spontaneously assembled into its holoproteins as proven on native polyacrylamide gels. Both recombinant H and L-chain ferritins were catalytically active in forming iron core. When the cells were cultured in the medium containing 10 mM ferric citrate, the cell-associated concentration of iron was 174.9 $\mu\textrm{g}$ Per gram(dry cell weight) for the recombinant yeast YG-L and 148.8 $\mu\textrm{g}$ Per gram(dry cell weight) for the recombinant yeast YG-L but was 49.4 $\mu\textrm{g}$ Per gram(dry cell weight) in the wild type, indicating that the iron contents of yeast is improved by heterologous expression of human ferritin H-chain or L-chain genes.

Relationship between Molecular Structure of Rice Amylopectin and Texture of Cooked Rice (쌀의 아밀로펙틴 분자구조와 밥의 텍스쳐)

  • Kang, Kil-Jin;Kim, Kwan;Kim, Sung-Kon
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.105-111
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    • 1995
  • The relationship betwwen the molecular structure of amylopectin and the texture of cooked rice was investigated using Korean rice [3 varieties of Japonica type and 3 varieties of Tongil type(Japonica-Indica breeding type)]. The molecular structure of rice amylopectin was polymodal and distributed A chain of $\overline{DP}$ 12.4, short B chain of $\overline{DP}$ 20.6, B chain of $\overline{DP}$ 26.3, long B chain of $\overline{DP}$ 45 and super long chain of above $\overline{DP}$ 55. The super long chain of amylopectin was composed of long linear chain with poorly branched chain. Also, the super long chain of amylopectin showed positive correlated with average chain length, inherent viscosity and ${\beta}-amyloysis$ limit$({\%})$, but negative correlated with ${\lambda}max$ of iodine reaction of amylopectin. The structural properties of amylopectin in Japonica type were different from those of amylopectin in Tongil type. In relationship between molecular structure of amylopectin and texture of cooked rice, the average chain length, inherent viscosity, ${\beta}-amyloysis$ limit and super long chain of amylopectin was showed a positive correlation with hardness, but a negative correlation with adhesiveness of cooked rice. The long chain of rice amylopectin is the less, the eating quality of cooled rice was the better. These results suggest that the molecular structure of rice amylopectin could be responsible for the texture of cooked rice.

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E-mail System Model based on Ethereum (이더리움 기반 이메일 시스템 모델)

  • Kim, Taekyung
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.13 no.4
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    • pp.99-106
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    • 2017
  • With the advent of virtual money such as bit coins, interest in the block chain is increasing. Block Chain is a technology that supports Distributed Ledger and is a versatile technology applicable to various fields. Currently, the block chain is conducting research for various applications such as virtual money, trade finance, marketplace, power market, image contents service, and IoT. The technologies that make up the block chain are smart contract, digital signature/hash function and consensus algorithm. And these technologies operate on P2P networks. In this paper, we have studied e-mail system based on the ethereum which is one of the block chain based technologies. Most legacy mail systems use SMTP and the POP3/IMAP protocol to send and receive e-mail, and e-mail use S/MIME to protect the e-mail. However, S/MIME is vulnerable to DDoS attacks because it is configured centrally. And it also does not provide non-repudiation of mail reception. To overcome these weaknesses, we proposed an e-mail system model based on ethereum. The proposed model is able to cope with DDoS attack and forgery prevention by using block chain based technology, and reliable recording and management among block chain participants are provided, so that it is possible to provide a non-repudiation function of e-mail transmission and reception.

DEVS-Based Simulation Model for Optimization of Sensor-Tag Operations in Cold Chain Systems (콜드체인 시스템의 센서태그 운영 최적화를 위한 DEVS 기반 시뮬레이션 모델)

  • Ryou, Okhyun;Kang, Yong-Shin;Jin, Heeju;Lee, Yong-Han
    • Journal of Korean Institute of Industrial Engineers
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    • v.41 no.2
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    • pp.173-184
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    • 2015
  • The application of radio frequency identification (RFID) sensor-tags in cold chain systems has recently received a great deal of attention. To design cold chain systems with RFID sensor-tags that minimize the initial investment and operational cost while fulfilling the functional and operational requirements, simulation study is one of the preferable and effective approaches. To simulate the possible design configurations, the individual components in a cold chain system can be extracted and implemented as a DEVS (Discrete Event System Specification) model. Based on the proposed DEVS model, a new cold chain simulation model can be efficiently created by simply connecting each DEVS model around the RFID sensor-tag of interest in sequence according to the structure of the cold chain system, and then executed (or simulated) on Java programming environments by the DEVSJAVA simulator. As a result of simulation, some key performance indexes such as reliability, accuracy or timeliness can be calculated and used to choose better components or to compare different system configurations of cold chain systems.

A Study on the Supply Chain Security Program (수출입 공급망 안전 프로그램에 관한 연구)

  • Han, Byoung Wan
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.58
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    • pp.287-311
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    • 2013
  • In this study, the scope of previous logistics security were focused only on port and ship. Because of it now extends to the overall (export and import) supply chain areas and in regards with supply security programs in the international level, it reviewed supply chain security programs categorized them into 'ships and port security system', 'container screening system', 'logistics chain authorization system' which are expanded to be adopted in the international level. The major features of those programs are summarized as in building risk management system, providing information ahead, selectivity test and benefits to AEO authorized companies in the customs administration level. The government and companies which are to ensure supply chain security and trade facilitation in order to cope actively with international customs administrative atmosphere need to do the followings : First, they need to build an intra-government integrated supply chain security and make efforts to conclude AEO MRA in order to increase trade competitiveness among major trading countries. Second, they need to build supply chain risk management system in order to enhance management performance through overseas market and company level strategy to obtain and maintain AEO authorization in the company level.

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A Review on Triple-A Supply Chain Performance

  • Ishaq, Muhammad Ishtiaq;Hussain, Nazia;Khaliq, Waseem;Waqas, Muhammad
    • Asian Journal of Business Environment
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    • v.2 no.2
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    • pp.35-39
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    • 2012
  • Purpose - Today's world is rapidly changing due to swift changes in organization's infrastructures and supply chain is becoming an important factor which affects the organization's global competitiveness. Research design / data / methodology - Researchers indicated that environment uncertainty is considered an external driving force to establish a unique supply chain. Market competition, reducing the gap of performance and quality between the products are compelling the practitioners and researcher to change the paradigm of managing the business processes effectively and efficiently. Results - In old times, supply chain of any organization was measured on the basis of inventory turns, defect rats, lead times, stock buffering and service level. But, Lee H.L. in 2004, published an article in Harvard Business Review who proposed three A (Adaptable, Agile and Aligned) termed as "Triple-A" of successful supply chain. Conclusions - He claimed that organizations can compete through the creation of Triple-A supply chain. Hence, the basic aim of this review paper is to develop basic insights regarding what is Triple-A of supply chain, its importance and functioning in today's competitive world.

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An Exploratory Treatise on Consciousness and Espousal of Halal Supply-Chain: An Indian Perspective

  • Potluri, Rajasekhara Mouly;Lee, Jung Wan;Potluri, Lohith Sekhar
    • The Journal of Asian Finance, Economics and Business
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    • v.4 no.2
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    • pp.69-74
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    • 2017
  • The purpose of this research is to be acquainted with the awareness and approval of halal supply chain among Indian manufacturers and distribution network members for haulage and warehousing activities from the perception of respective service suppliers. A total of 20 respondents, which consist of 10 transportation companies and 10 warehousing companies from the State of Andhra Pradesh in India were selected for the study by using purposive sampling method. The principal focal points of the discussions are on awareness and adoption of halal transportation and warehousing services chosen for the study in the comprehensive halal supply chain. A total of 90 percent and 70 percent of respondents from the transportation and warehousing companies respectively agreed that they know only about the concept of halal but do not have any exposure and ken on the halal supply chain. However, findings of this research won't have extensive validity in the market, gaining an enough familiarity with the halal supply chain in the Indian social context is of immense importance. This is a pioneering attempt aimed to investigate the awareness and adoption levels of halal supply chain among Indian businessmen which are precious for supply chain companies to customize their services in the country as well to the world of academia.

The Information System Management and Its Infrastructure for Supply Chain Management as Antecedents of Financial Performance

  • MUNEER, Saqib
    • The Journal of Asian Finance, Economics and Business
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    • v.7 no.1
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    • pp.229-238
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    • 2020
  • A model is presented in this paper to provide understanding of the supply chain integration and supply chain information practices' impact on the manufacturing industries. The supply chain information practices play a crucial role in sharing information between the members of SC network. Thus, it is important to develop a comprehensive understanding of the differences and similarities among ISI and information management. It will allow firms to systematically evaluate and carefully choose the information strategy. The empirical findings of this research offer essential and interesting insights about what role SCI, supply chain information and Supply chain ISI play in determining Malaysia's financial performance. The theoretical gaps addressed in this study are of significant importance, since a little empirical evidence is available regarding system infrastructure and supply chain information management's effectiveness. This research provides further paths of exploring system infrastructure and information management, thereby defining the manufacturing industries' next step in SCM struggle i.e. modifying total integrated SC principle in other manufacturing firms. The Resource-based theory discovered organizational resources as an essential organizational success ingredient. Therefore, in order to recognize its potential value, internal resources, for instance, information system and management must be fully utilized.

Molecular Dynamic Simulations of the Fatty Acid Bilayer Containing Very Long Chain Transmembrane Dicarboxylic Acids

  • Choi, Yong-Hoon;Yang, Chul-Hak;Kim, Hyun-Won;Jung, Seun-Ho
    • BMB Reports
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    • v.33 no.1
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    • pp.54-58
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    • 2000
  • Recent research results regarding the very long chain transmembrane ${\alpha},{\omega}-dicarboxylic$ components in the membrane of extremophilic eubacteria, such as Sarcina ventriculi, Thennotoga maritima, and Thermoanaerobacter ethanolicus have raised interesting questions concerning the physical and biochemical function on these components in the membrane. In order to understand the dynamic characteristics of these acids which reside in the bilayer membrane, 580 ps molecular dynamic simulations at 300 K were performed for two model systems. These systems were the bilayer with regular chain (C16:0 or C18:1) fatty acid methyl esters and the fatty acid bilayer containing very long chain transmembrane dicarboxylic acid methyl esters (${\alpha},{\omega}-15,16-dimethyltriacotane-dioate$ dimethyl ester; C32:0). Our analyses indicate that very long chain transmembrane dicarboxylic acids have a noticeable influence on the bilayer dynamics at a sub-nanosecond time scale. The center-ofmass mean-squared-displacement (MSD) of regular chain fatty acids adjacent to the very long chain transmembrane dicarboxylic acids decreased, the long-axis order parameter increased, and the reorientational motions of methylene groups were slowed along the hydrocarbon chains. These results indicate that the very long chain transmembrane dicarboxylic acids reduce the molecular order of the whole bilayer membrane.

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Application of Consignment to Three Stage Supply Chain

  • Ryu, Chungsuk;Hwang, Gyuyoung
    • Journal of Distribution Science
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    • v.16 no.7
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    • pp.35-45
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    • 2018
  • Purpose - The study investigates the impact of consignment on the economic performance in the supply chain with three stages. Through the analysis on distinct forms of consignment application, this study intends to answer to the question of how the consignment should be used in the multi-stage supply chain. Research design, data, and methodology - The proposed mathematical model represents the supply chain system with a manufacturer, a wholesaler, and a retailer. Three different forms of consignment application are considered depending on which stages adapt the consignment, and their system profits are compared with the traditional non-consignment system in numerical examples. Results - The numerical examples show that the serial consignment application performs better than any other forms of consignment as well as the non-consignment system. The additional analysis indicates that the system profit is significantly sensitive to the consignment rate. Conclusions - The outcome of this study implies the potential of consignment to improve the system performance even in the multi-stage supply chain system. Meanwhile, each supply chain member's preference to the specific form of consignment application could be different depending on which stage he has. All the supply chain members should jointly determine the appropriate consignment rates to obtain the best system performance.