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Cloning of Low-molecular-weight Glutenin Subunit Genes and Identification of their Protein Products in Common Wheat (Triticum aestivum L.) (보통 밀에서 저분자글루테닌 유전자 클로닝 및 단백질 동정)

  • Lee, Jong-Yeol;Kim, Yeong-Tae;Kim, Bo-Mi;Lee, Jung-Hye;Lim, Sun-Hyung;Ha, Sun-Hwa;Ahn, Sang-Nag;Nam, Myung-Hee;Kim, Young-Mi
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.547-554
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    • 2010
  • Low-molecular-weight glutenin subunit (LMW-GS) in common wheat (Triticum aestivum L.) is important for quality processing of bread and noodles. The objectives of this study were to clarify the composition of LMW-GSs and to identify their corresponding proteins. Using LMW-GS specific primers we cloned and characterized 43 LMW-GS genes in the wheat cultivar 'Jokyoung'. Some of these genes contain polypeptides different in size due to the presence of various deletions or insertions within repetitive and glutamine-rich domains. The comparison of deduced amino acid sequence of the LMW-GS genes in Jokyoung with that of 12 groups LMW-GSs of wheat cultivar Norin 61 showed that the deduced amino acid sequences were nearly the same to LMW-GS groups of 1, 2, 3/4, 5, 7, 10 and 11. All LMW-GS genes contain eight cysteine residues, which are conserved among all of the typical LMW-GS sequences. The relative positions of cysteine residues are also conserved, except those of the first and seventh. Based on phylogenetic analysis, the 43 sequences with the same N-terminal and C-terminal amino acid sequences were clustered in the same group. To identify the proteins containing the corresponding amino acid sequences, we determined the N-terminal amino acid sequence of 7 spots of LMW-GSs of Jokyoung separated by two-dimensional gel electrophoresis (2DE). Of them, Glu-B3 (LMW-m and LMW-s) and Glu-D3 (LMW-m) were detected in two and three spots, respectively and the others were not clear. Collectively, we classified diverse LMW-GSs and identified their corresponding protein products. These results will be helpful in breeding programs for improvement of wheat flour quality.

A Case Study of 2-year-old Infants' Attachment Security to a Child Care Teacher and Peer Play Process (보육교사에 대한 만 2세 영아의 애착 안정성과 또래놀이 과정에 관한 사례연구)

  • Shin, Dong Ju;Kim, So Young
    • Korean Journal of Child Education & Care
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    • v.18 no.4
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    • pp.1-25
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    • 2018
  • Purpose: The purpose of this study was to explore about 2-year-old infants' attachment security to a child care teacher and peer play process through a case study. For this purpose, the attachment security was assessed with attachment Q-set to 2-year-old five infants at a day care center in Seoul. Participants of this study are four infants included two infants attached securely to their child care teacher and two infants attached insecurely to their child care teacher. Methods: Participant observation was conducted from April 12 to July 19 in 2018 through 36 observations in free play activities. The Data collected from the transcriptions of video shoots and teachers' interviews, child care plans, and reflexive journal was analyzed and interpreted. Results: The main results of the research were as follows: The beginning of peer play, when infants invited their peers to play, secure attachment infants selected suitable play partners and asked for a specific play behaviors. However, insecure attachment infants invited unsuitable play partners and asked uncertainly play actions to peers. In addition, when infants entered in-progress play, secure attachment infants understood the context of play but insecure attachment infants did not understand the context of play. In the progress of peer play, secure attachment infants cooperated with peers, shared play objects, but insecure attachment infants did not cooperate complementally with peers and express only their opinions. As well, insecure attachment infants could not play harmoniously without child care teacher's interventions. In the end of the peer play, secure attachment infants finished in collaboration with peers but insecure attachment infants failed to finish with the peers and was turned into play with the child care teacher because of peer conflict. Conclusion/Implications: This study has implications for basic resources to think about the role of child care teachers by helping them understand about the relation 2-year-old infants' attachment security to them and peer play process.

A Study on the Application Effect of Central-Grid PV System at a Streetlamp using RETScreen - A Case Study of Gwangjin-gu - (RETScreen을 이용한 가로등의 계통연계형 태양광시스템 적용 효과 분석 - 서울시 광진구를 중심으로 -)

  • Kang, Seongmin;Choi, Bong-Seok;Kim, Seungjin;Mun, Hyo-dong;Lee, Jeongwoo;Park, Nyun-Bae;Jeon, Eui-Chan
    • Journal of Climate Change Research
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    • v.5 no.1
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    • pp.1-12
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    • 2014
  • With continued economic growth, Korea has seen an increase in the nighttime activities of its citizens as hours of activity have extended into night. There is an increasing trend in energy consumption related to citizens' nighttime activities. In order to analyze ideas for an efficient replacement of the power consumption of streetlights and for profit generation by applying grid-type solar systems, this study used an RETScreen model. Through energy analysis and cost analysis, the application benefit and viability of grid-type solar street light systems were analyzed. With analysis result of a total weekly power generation of 114 kWh via a grid-connected solar streetlight system, it was shown that the net present value of a grid-connected solar street light system is 155,362 KRW, which would mean a payback period of about 5.2 years, and as such, it was shown that profit could be generated after about 6 years. In addition, if the grid-connected solar power generation system proposed by this study is to be applied, it was shown that 401,935 KRW in profit could be generated after the 20-year useful life set for the solar system. In addition, the sensitivity analysis was performed taking into account the price fluctuations of SMP, maintenance. As a result, a payback period has increased by 1~2 years, and there were no significant differences. Because the most important factor that affect the economic analysis is the cost of supply certification of renewable energy, a stable sales and acquisition of this certification are very important. the Seoul-type Feed in Tariff(FIT) connected to other institutions will enable steady sales by confirming to purchase the certification for 12 years. Therefore, if those issues mentioned above are properly reflected, Central-grid PV system project will be able to perform well in the face of unfavorable condition of solar PV installation.

A study on the Cooking Vessel of Baekje Hanseong Period (백제 한성기 취사용기에 대한 검토 - 심발형토기와 장란형토기를 중심으로 -)

  • Jung, Su Ock
    • Korean Journal of Heritage: History & Science
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    • v.44 no.4
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    • pp.112-129
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    • 2011
  • This paper was written to understand, through the types and production techniques, how cooking vessels of Baekje Hanseong period had evolved, - the cooking vessels that were excavated from the historic sites of Baekje Hanseong Period such as Mongchontoseong and Pungnaptoseong of Seoul, the ancient tomb group of Seokchon-dong, and Misari historic site of Hanam. First, the results produced through the analysis of types of these cooking vessels are as followings: as for deep bowls, the maximum body diameter is found on the top of pottery, while as for an egg-shaped pottery, the maximum body diameter is found on the middle height of pottery. However, as for the rim diameter ratio to the neck diameter ratio of pottery, the said two typed potteries were shown to have smaller ones. In consideration of each historic site, as for deep bowls, the ancient tomb group of Seokchon-dong had potteries that had less volumes, were deeper, and had higher maximum body diameters. When it comes to an egg-shaped pottery, Pungnaptoseong had the potteries that were deeper than those of Misari historic site, and their potteries also had the maximum body diameter found on the middle height of pottery, and larger rim diameter ratio to the neck diameter of the pottery. The results produced though the analysis of the production techniques of these cooking vessels are as followings: in the case of deep bowls, as the width of a volume category gets larger, the pottery stamping with an anvil including patterns was more frequently used for pottery production. In this case, simultaneously braid patterns were frequently found on the outer sides of these potteries. In addition, it was found the basal walls of these potteries were thinnest in all typed potteries. This shows there occurred technique transitions according to the time flow. When it comes to an egg-shaped pottery as well, the pottery produced by using an anvil had the thinnest basal wall and its rim diameter ratio to its neck diameter ratio was smaller. Also around the mouth of this pottery, traces were found on its front, the traces formed by the strong rotating power. It is determined these two typed potteries were all produced by using an anvil to have thinner walls. Then, how these potteries were used? In the case of deep bowls, liquid food was put on a hearth or ground to be cooked whereas in the case of an egg-shaped pottery, the pottery was put on a puttumak(kitchen stove) mainly for boiling water. The deep bowls and egg-shaped potteries excavated from the center areas of Baekje Hanseong Period were not clearly determined about when their pastes or firing times were, but as for their production techniques, it was possible to check how they had developed. Moreover, it was determined that in the changing aspects of their production techniques, temporalite is also reflected.

Informed Consent and Refusal of Treatment in Emergency Medical Situation (응급의료에서의 설명·동의 원칙과 응급의료거부죄)

  • Lee, Jung-eun
    • The Korean Society of Law and Medicine
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    • v.23 no.1
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    • pp.37-80
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    • 2022
  • By analyzing informed consent and the refusal of emergency medical treatment (called patient dumping) under the current Emergency Medical Service Act, this study suggests that an emergency medical professional is only liable for patient dumping if their duty to protect the patient's life takes precedence over the patient's right to self-determination. In emergency medical situations, as in general medical situations, medical treatment should be performed after the emergency medical professional informs the patient about the medical treatment, including its necessity and methods, and obtains consent from the patient. Refusing or evading the performance of emergency medical services on the excuse of the informed consent not considering a waiver or alteration of informed consent requirements without reasonable reasons violates the Emergency Medical Service Act and thus makes an emergency medical professional liable to administrative disposition or criminal penalty. In other words, depending on the existence of a waiver of alteration of the informed consent, patient dumping may be established. If the patient is a minor or has no decision-making ability, and their legal representative makes a decision against the patient's medical interests, the opinion of the legal representative is not unconditionally respected. A minor also has the right to decide over their body, and the decisions of their legal representatives should be in the patient's best interests. If the patient refuses treatment, in principle, the obligation of life protection of emergency medical professionals is the top priority. However, making these decisions in the aforementioned situations in the emergency medical field is difficult because of the absence of explicit regulations regarding these exceptional problems. This study aims to organize the following precedents of the Supreme Court of Korea. The court states that, when balancing the conflicting interests between the duty to provide emergency medical service and the duty to inform is unavoidable for emergency medical professionals, they should put the duty to protect the patient's life ahead of the duty to inform if the patient's life matters. Exceptionally, when a patient has seriously considered whether they should receive treatment before the emergency medical situation, their right to self-determination can be considered equal to the obligation of emergency medical professionals to provide emergency medical treatment. This research also suggests that an amendment of the Emergency Medical Service Act should include the following. First, the criteria for determining the decision-making ability of emergency patients should consist of medical content. Second, additional consent from a medical professional is unnecessary for first-aid treatment. Finally, new provisions for emergency medical obligations for minors, new provisions for the decision standard when there are conflicting opinions about the treatment of a patient, and new penalty provisions for professionals who suspend emergency medical examinations and treatments need to be established.

A Study on the in Improvement of Elder Abuse through Analysis the Causes and the Type of Elder Abuse the Super-aged Society (초고령사회 노인학대의 발생원인 및 유형 분석을 통한 개선방안 연구)

  • Park, Hyun-Seung
    • Industry Promotion Research
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    • v.7 no.1
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    • pp.35-42
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    • 2022
  • The purpose of this study was to establish an Improvement Plan to determine the cause of the abuse occurred for the elderly who live in urban areas, and analyzed by type. This study was conducted to survey targeted the more than 65-year-old man as the center of Seoul Mapo area. The independent variables in the research model is elder abuse causes, the sociological characteristics as control variables population was the dependent variable in the elderly abuse. As a result; First, investigate the differences in the cause and the cause of elder abuse according to demographic characteristics. The causes of elder abuse, age of the higher age group showed high levels of elder abuse causes. Showed that less than a high school education than graduate school. Second, investigate the factors influencing the occurrence of elder abuse experiences of the subjects. Personal factors of elder abuse causes of those surveyed, family environmental factors, social, and refers to the result that the higher the level of cultural factors increase the occurrence of elder abuse experience. Third, investigate the differences in the cause and the cause of elder abuse according to demographic characteristics. The causes of elder abuse, age of the higher age group showed that the level of elder abuse occurs and causes high experience. Fourth, investigate the factors influencing the occurrence of elder abuse experiences of the subj ects. This personal factors of elder abuse causes of those surveyed, family environmental factors, social, and refers to the results of the higher levels of cultural factors that increase the occurrence of elder abuse experience. In conclusion, elder abuse is personal factors, environmental factors, family, social and cultural factors, the higher the level can be seen that type of elder abuse Elder abuse occurs, formed by many, accordingly. Therefore, in order to improve elder abuse should be healing the cause according to the type of elder abuse appears essentially as a result from this research.

Yearly Update of the List of Plant Diseases in Korea (6.2 Edition, 2024) (한국식물병명목록의 연간 현황 보고(6.2판, 2024년 개정본))

  • Jaehyuk Choi;Seon-Hee Kim;Young-Joon Choi;Gyoung Hee Kim;Ju-Yeon Yoon;Byeong-Yong Park;Hyun Gi Kong;Soonok Kim;Sekeun Park;Chang-Gi Back;Hee-Seong Byun;Jang Kyun Seo;Jun Myoung Yu;Dong-Hyeon Lee;Mi-Hyun Lee;Bong Choon Lee;Seung-Yeol Lee;Seungmo Lim;Yongho Jeon;Jaeyong Chun;Insoo Choi;In-Young Choi;Hyo-Won Choi;Jin Sung Hong;Seung-Beom Hong
    • Research in Plant Disease
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    • v.30 no.2
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    • pp.103-113
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    • 2024
  • Since 2009, the Korean Society of Plant Pathology has established the Committee on Common Names of Plant Disease to systematically review and determine plant disease names and related terminologies. The committee published the 6th edition of the List of Plant Diseases in Korea (LPDK) in 2022, and the list has been made publicly accessible online. The online database has significantly enhanced user accessibility, expedited update processes, and improved interoperability with other databases. As a result, the 6.1 edition of the list was released by online LPDK in 2023, detailing new disease names added over the preceding year and revisions to existing names. Subsequently, in 2024, the 6.2 edition was published, encompassing 6,765 diseases caused by 2,503 pathogen taxa across 1,432 host species. The public release of the online database has, however, introduced several challenges and tasks. Addressing these issues necessitates the development of modern, standardized nomenclature guidelines and a robust system for the registration of new disease names. Open communication and collaboration among the diverse members of the Korean Society of Plant Pathology are required to ensure the reliability of the LPDK.

Dental Hygienists' Awareness of Long-term Senior Care Insurance System (노인장기요양보험제도에 관한 치과위생사의 인지도)

  • Kim, Ji-Hyun;Hwang, Ji-Min;Park, Yong-Duk
    • Journal of dental hygiene science
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    • v.10 no.1
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    • pp.39-44
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    • 2010
  • The purpose of this study is to cover dental hygienists who work for metropolitan dental hospitals or clinics in Seoul city, Incheon city and Gyeonggi province from January to April 2009 and profile their awareness of Long-term Senior Care Insurance System (hereinafter called 'LSCI (System)', so that it may provide material reference data to contribute to expanding and establishing oral health medical services in the framework of LSCI System. As a result, this study could come to the following conclusions: 1. It was found that the highest priority of dental hygienists' visiting oral hygienic services under LSCI System was focused on 'caring and preventive treatment', and their secondary priority was focused upon 'oral health education.' 2. In response to a question item about whether dental hygienists need dentist's prescription in written before performing their visiting oral hygienic services, it was found that 38.2% dental hygienists answered 'Yes (necessary)' and 61.8% answered 'No (unnecessary).' 3. In response to a question item about whether dental hygienists may open up long-term senior care center, it was found that absolute majority of dental hygienists (93.4%) answered 'No' and only 6.6% dental hygienists answered 'Yes.' The standardized professional education are thought to be needed to be developed aiming at the success in oral-hygiene service within a LSCI, by strengthening professionalism in dental hygienists.

A Study of a Pattern of the Stress Perceived by Stroke Patients through the Rehabilitative Process (뇌졸중 환자의 재활과정에 따른 스트레스 변화 양상)

  • Lee, Jung-Min
    • Journal of East-West Nursing Research
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    • v.1 no.1
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    • pp.82-98
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    • 1997
  • The purpose of this study was to determine pattern of the stress perceived by stroke patients over time. The ultimate goal of the research is to provide data to help nurses to design the plan of nursing care of the stroke patients both in the hospital and at home. A total of 57 admitted stroke patients were collected from one general hospital in Seoul from June, 12 to September, la, 1993. The data were collected for three phases(within one week after leaving the hospital). The tools for this study, three scales were used ; Stress scale developed by the investigator. Constitution classifing scale designed by Kho(1984), and Self-care measuring scale by Kang(1984). Data were analyzed in four steps using statistical analysis. First, demographic data were determined by descriptive statistics. Second. the pattern of stress perceived by stroke patients across three phases was measured using repeated measures ANOVA. Third, stress of stroke patients classified by constitution, paralyzed area. and attack frequency were measured using ANOVA or t-test, and the pattern of stress by group over time was determined using paired t-test in post hoc test. Fourth. Pearson correlation coefficients were calculated to determine the relationship between the stress and self-care activities. The results of this study are ; 1. The pattern of stress across three phases ; There was a decrease of the stress across three phases. In general. psychological stress as the highest among three phases(F=36.92. P=.000). There was a statistically significant difference of the physical stress(F=34.55, p=.000), the psychological stress (F=15.49, p=.0005) and the social stress (F=24.71. p=.000) among three phases. There was a statistically significant difference of the stress between the first phase (on admission) and the second phase(before leaving the hospital) and was a decrease of the stress (t =6.36. p=.000). 2. The pattern of stress of stroke patients classified by constitution across three phases ; Stroke patients classified as So-Eum perceived the highest stress among three groups(Tae-Eum, So-Eum. So-Yang). There was no statistically significant difference of stress according to the constitution of stroke patients among three phases. Hence. stress was not influenced by the constitution of stroke patients, but there was a statistically significant difference of stress over time. 3. The pattern of stress of stroke patients classified by the paralyzed area across three phases ; Right paralyzed stroke patients perceived higher stress than left paralyzed stroke patients. There was, however, no statistically significant difference of stress between two groups except 2nd phase. There was no statistically significant difference of the perception of stress bet ween the right and left paralyzed stroke patients. 4. The pattern of stress of stroke patients classified by the frequency of the relapse of the disease across three phases ; Stress was higher in stroke patients who had the relapse of the disease twice more than the first time. There was, however, no statistically significant difference of stress between two groups. There was no statistically significant difference of stress of stroke patients according to the relapse of the disease among three phases. Hence, stress was no influenced by the relapse of the disease. 5. The relationship between the stress and self-care activities ; There was a negative relationship between the stress and self-care activities each phase(on admission, r= -.1563 ; before leaving the hospital, r= -.4030 ; after leaving the hospital, r= -.5291). Hence, the higher the self-care activities, the lower the stress. This study has three important findings. First finding was that psychological stress perceived by stroke patients was the highest among three phases. The second finding was that factors such as the constitution, the paralyzed area, and the relapse of the disease did not have an influence on the stress perceived by stroke patients across three phases(on admission, before leaving the hospital, after leaving the hospital). There was a statistically significant decrease of the stress perceived by stroke patients across three phases. The third finding was that there was a negative relationship between the self-care ability and stress. In this study, these findings have implications for nursing care for the rehabilitation of stroke patients and suggest the need of nursing intervention to promote the self-care ability and to support the psychological self-esteem of stroke patients.

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Estimation of Temporal Surface Air Temperature under Nocturnal Inversion Conditions (야간 역전조건 하의 지표기온 경시변화 추정)

  • Kim, Soo-ock
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.19 no.3
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    • pp.75-85
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    • 2017
  • A method to estimate hourly temperature profiles on calm and clear nights was developed based on temporal changes of inversion height and strength. A meteorological temperature profiler (Model MTP5H, Kipp and Zonen) was installed on the rooftop of the Highland Agriculture Research Institute, located in Daegwallyeong-myeon, Pyeongchang-gun, Gangwon-do. The hourly vertical distribution of air temperature was measured up to 600 m at intervals of 50 m from May 2007 to March 2008. Temperature and relative humidity data loggers (HOBO U23 Pro v2, Onset Computer Corporation, USA) were installed in the Jungdae-ri Valley, located between Gurye-gun, Jeollanam-do and Gwangyang-si, Jeollanam-do. These loggers were used to archive measurements of weather data 1.5 m above the surface from October 3, 2014, to November 23, 2015. The inversion strength was determined using the difference between the temperature at the inversion height, which is the highest temperature in the profile, and the temperature at 100 m from the surface. Empirical equations for the changes of inversion height and strength were derived to express the development of temperature inversion on calm and clear nights. To estimate air temperature near the ground on a slope exposed to crops, the equation's parameters were modified using temperature distribution of the mountain slope obtained from the data loggers. Estimated hourly temperatures using the method were compared with observed temperatures at 19 weather sites located within three watersheds in the southern Jiri-mountain in 2015. The mean error (ME) and root mean square error (RMSE) of the hourly temperatures were $-0.69^{\circ}C$ and $1.61^{\circ}C$, respectively. Hourly temperatures were often underestimated from 2000 to 0100 LST the next day. When temperatures were estimated at 0600 LST using the existing model, ME and RMSE were $-0.86^{\circ}C$ and $1.72^{\circ}C$, respectively. The method proposed in this study resulted in a smaller error, e.g., ME of $-0.12^{\circ}C$ and RMSE of $1.34^{\circ}C$. The method could be improved further taking into account various weather conditions, which could reduce the estimation error.