• Title/Summary/Keyword: center in Seoul

Search Result 11,613, Processing Time 0.05 seconds

Development of Detection Method for Cyclomaltodextrinase Family Genes using Degenerate PCR Primers

  • Oh, Su-Won;Jang, Myoung-Uoon;Jeong, Chang-Ku;Yuk, Jeong-Bin;Park, Jung-Mi;Park, Kwan-Hwa;Kim, Tae-Jip
    • Food Science and Biotechnology
    • /
    • v.15 no.6
    • /
    • pp.967-974
    • /
    • 2006
  • Cyclomaltodextrinases (CDases), maitogenic amylases, and neopullulanases share highly conserved primary structures and similar characteristics, and are thus classified into the same family. BLAST search has showed that a variety of bacterial strains harbor putative CDase family genes with several well-conserved motif amino acid sequences. In this study, four degenerate polymerase chain reaction (PCR) primer sets were designed for the detection of CDase genes, on the basis of their highly conserved amino acid blocks (WYQIFP, DGWRLD, LGSHDT, and KCMVW). The PCR detection conditions were optimized and the detection specificity of each for the primer sets was tested against the genomic DNAs isolated from 23 different Bacillus-associated species. Consequently, all tested primer sets evidenced successful amplification of specific PCR products in length, which share 55-98% amino acid sequence identity with known and putative CDases. The primers developed herein, therefore, can be applied for the easy and efficient detection and isolation of CDase family genes for the modification of functional food carbohydrates.

The Effect of Mother's Depression and Parenting Behavior on Children's Problem Behavior (어머니의 우울과 양육행동이 유아의 문제행동에 미치는 영향)

  • Lee, So Hyun
    • Korean Journal of Child Education & Care
    • /
    • v.18 no.2
    • /
    • pp.117-127
    • /
    • 2018
  • The purpose of this study was to analyze the relationships among mother's depression, parenting behavior of children's problem behavior. The subjects used in this study were 203 three to five-year-old children who attended kindergarten and deycare center in Seoul and Gyonggido. The research data were collected through the 'Beck Depression Inventory(BDI)', 'Maternal Behavior Research Instrument(MBRI)', 'K-CBCL'. The data were analyzed by Pearson's correlation and Stepwise Multi-Regression. The results of this study indicate that there were significant positive correlations among children's problem behavior, mother's depression, rejective and control parenting behavior. And there were significant negative correlations among children's problem behavior, mother's affectionate and permissive parenting behavior. Also, the mother's depression was the predicted variable that has the most significant relative effect on children's problem behavior. Moreover, the results of this study indicate that rejective parenting behavior that is subordinate factor of parenting behavior was additional predictor of stress.

Comparison of three different surface plank exercises on core muscle activity

  • Lee, Jin;Jeong, Kwanghyun;Lee, Hyuna;Shin, Jaeyeon;Choi, Jaelim;Kang, Seungbeom;Lee, Byoung-Hee
    • Physical Therapy Rehabilitation Science
    • /
    • v.5 no.1
    • /
    • pp.29-33
    • /
    • 2016
  • Objective: This study compared the muscle activities of the erector spinae (ES), the external oblique (EO), and the rectus abdominis (RA) on three different surfaces. The purpose of this study was to determine which surface induces the highest muscle activity during the plank exercises. The information from this study can be used to recommend plank exercises to athletes and patients with weak core muscles. Design: Cross-sectional study. Methods: The subjects include 20 adult males attending S University in Seoul. Participants completed each plank exercise on three different surfaces. To measure muscle activities, researchers used the values from electromyography. The measurement excluded the initial two and final two seconds and collected information on the RA, EO, and ES in each posture of each subject. Results: The left external oblique showed significant differences between the plank position on stable ground (ST) and the plank position using a suspension device (SL) (p<0.05) and between the plank position on the unstable ground (US) and SL (p<0.05). The right rectus abdominis and left rectus abdominis displayed statistically significant differences between the ST and the US (p<0.05) and between the ST and the SL (p<0.05). The right erector spinae had a statistically significant difference between ST and US (p<0.05). Conclusions: The plank exercise strengthens the core muscles effectively, and muscle activity is related to the posture of the exercise and the location of the muscle. These results suggest that plank exercises improve muscle activities. Additionally, plank exercises can be applied to general medical care.

Comparison of Propacetamol plus Fentanyl and Fentanyl alone with Patient Controlled Analgesia after Total Knee Arthroplasty (수술 후 자가통증조절장치 사용 환자에서 propacetamol과 fentanyl 복합제 및 fentanyl 단일제제의 효과 비교)

  • Kim, Minhyung;Jeong, Hyokeun;Park, Sohyun;Rhie, Sandy Jeong
    • Korean Journal of Clinical Pharmacy
    • /
    • v.28 no.1
    • /
    • pp.17-23
    • /
    • 2018
  • Objective: Opioid analgesics, for postoperative pain management, are an indispensable group of medication; however, they also have a variety of adverse drug reactions (ADR). Multimodal methods, combining non-opioid analgesics with opioid analgesics, have been investigated to increase the effects of analgesics and reduce ADR with opioid-sparing effects. The purpose of this study was to compare the effects of patient-controlled analgesia (PCA) with fentanyl alone, and PCA with fentanyl and intravenous (i.v.) propacetamol to determine the effects of pain control, cumulative opioid usage, and opioid ADR. Methods: The subjects were patients who underwent total knee arthroplasty at the Seoul Veterans hospital from January 1, 2015 to December 31, 2016. The study period was from postoperative day 0 (POD0) to day 3 (POD3), and the retrospective study was conducted using electronic medical records. Results: Pain severity was significantly low at POD1 (p = 0.017), POD2 (p = 0.003), and POD3 (p = 0.002) in the multimodal group. The fentanyl only group frequently reported both moderate and severe pain at a statistically significant level. This was consistent with the analysis of the pro re nata (PRN) intramuscular analgesia usage at the time of numerical rating scale (NRS) 4 and above. The opioid-sparing effect confirmed that the average opioid dose equivalent to i.v. morphine dose was 9.4 mg more than that used for the multimodal group in the fentanyl only group. The ADRs and length of stay between the two groups were not statistically different. Conclusion: The results of this study suggest that the combination therapy of fentanyl and i.v. propacetamol is superior to fentanyl monotherapy.

DNP3 Protocol Security and Attack Detection Method (DNP3 프로토콜 보안 현황 및 공격 탐지 방안)

  • Kwon, Sung-Moon;Yoo, Hyung-Uk;Lee, Sang-Ha;Shon, Tae-Shik
    • Journal of Advanced Navigation Technology
    • /
    • v.18 no.4
    • /
    • pp.353-358
    • /
    • 2014
  • In the past, security on control system was guaranteed by isolation of control system networks from external networks. However as devices of the control systems became more various and interaction between the devices became necessary, effective management system for such network emerged and this triggered connection between control system networks and external system networks. This made management of control system easier but also made control system exposed to various cyber attack threats, Therefore researches on appending security measures on each protocols are in progress. This paper focused on DNP(distributed network protocol)3 protocol which is used for communication between control center and substations. It describes characteristics of DNP3 protocol and research on adding security elements to the protocol. It also analyzed known vulnerabilities of DNP3 protocol and proposed data mining methodology for detecting such vulnerabilities.

Use of alternative curing salts for processing salamis

  • Yim, Dong-Gyun;Chung, Ku-Young;Jo, Cheorun;Nam, Ki-Chang
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.31 no.1
    • /
    • pp.123-128
    • /
    • 2018
  • Objective: This study was performed to determine effects of different curing salts on the quality of salamis and to assess feasibility of using NaCl-alternative salts. Methods: Various types of curing salts (KCl or $MgCl_2$) as well as NaCl (sun-dried or refined) were incorporated for processing of salamis. The proximate composition, fatty acids, nucleotide-related compounds, and free amino acids of the salamis were analyzed during 40 days of ripening. Results: The substitution of NaCl by KCl caused higher fat and ash content, but lower moisture content of the salami after 20 days of ripening (p<0.05). Compared with the sun-dried NaCl, use of KCl in salami also led to greater inosine 5'-monophosphate whereas refined NaCl had more inosine (p<0.05). KCl-added salami also had a higher C12:0, C17:1, and C20:0 than other types of salami (p<0.05). $MgCl_2-added$ salami had higher content of free amino acids compared to the other salamis (p<0.05). Conclusion: Alternative curing salts such as KCl and $MgCl_2$ could substitute NaCl in consideration of quality factor of a fermented meat product. Especially replacement of NaCl with KCl will be a suitable strategy for developing relatively low sodium salami products without compromising product quality.

An Intimacy-based Trust Reasoning Method for Intelligent Ecommerce Systems (지능형 전자 상거래 시스템 구축을 위한 친밀도 기반 신뢰도 추론방법)

  • Kwon, Ohbyung;Park, Kwangho
    • The Journal of Society for e-Business Studies
    • /
    • v.18 no.2
    • /
    • pp.1-26
    • /
    • 2013
  • Estimating levels of user trust is important for maintaining continuous use of e-commerce systems because trust alleviates user concerns about the invisibility of service providers or their reputation. Conventional trust estimation approaches such as policy-and reputationbased reasoning have focused on the experience of e-commerce systems at an early stage. However, only a few trust reasoning methods have considered the mature stage, which is more related to continuance intention. We propose a trust reasoning method dedicated to the mature stage of using e-commerce systems. In particular, a new method of unobtrusively estimating the degree of user intimacy is developed, because intimacy has been highly associated with trust as well as reputation. Our experiments show that the proposed method is valid and can be used in conjunction with reputation-based trust reasoning.

PECAN: Peer Cache Adaptation for Peer-to-Peer Video-on-Demand Streaming

  • Kim, Jong-Tack;Bahk, Sae-Woong
    • Journal of Communications and Networks
    • /
    • v.14 no.3
    • /
    • pp.286-295
    • /
    • 2012
  • To meet the increased demand of video-on-demand (VoD) services, peer-to-peer (P2P) mesh-based multiple video approaches have been recently proposed, where each peer is able to find a video segment interested without resort to the video server. However, they have not considered the constraint of the server's upload bandwidth and the fairness between upload and download amounts at each peer. In this paper, we propose a novel P2P VoD streaming system, named peer cache adaptation (PECAN) where each peer adjusts its cache capacity adaptively to meet the server's upload bandwidth constraint and achieve the fairness. For doing so, we first propose a new cache replacement algorithm that designs the number of caches for a segment to be proportional to its popularity. Second, we mathematically prove that if the cache capacity of a peer is proportional to its segment request rate, the fairness between upload and download amounts at each peer can be achieved. Third, we propose a method that determines each peer's cache capacity adaptively according to the constraint of the server's upload bandwidth. Against the proposed design objective, some selfish peers may not follow our protocol to increase their payoff. To detect such peers, we design a simple distributed reputation and monitoring system. Through simulations, we show that PECAN meets the server upload bandwidth constraint, and achieves the fairness well at each peer. We finally verify that the control overhead in PECAN caused by the search, reputation, and monitoring systems is very small, which is an important factor for real deployment.

Synergy Effect of Sun Protection Factor Using Method of Forming Self-Assembly of Hybrid Titanium Dioxide (하이브리드 이산화티탄의 자기조직체 형성공법을 이용하여 제조된 하이브리드 이산화티탄의 자외선차단 상승효과)

  • Cho, Hyun Dae;Park, Soo Nam
    • Journal of the Korean Applied Science and Technology
    • /
    • v.31 no.4
    • /
    • pp.748-758
    • /
    • 2014
  • The purpose of this study is to find the optimum conditions for manufacturing titanium dioxide using a hybrid self-assembly forming method, to confirm the shape, properties and synergy effect of UV protection for hybrid titanium dioxide. Hybrid titanium dioxide, manufactured by forming self-assembly of different sizes consisting of two kinds of titanium dioxides, has micro titanium dioxide (250nm~300nm) for support material, Nano titanium dioxide (20~30nm) for surface material, coating support material. Adjustment experiments of $AlCl_3$ concentration and both titanium dioxide ratio were conducted to find the optimized conditions for the surface coating of titanium dioxide striking a negative charge, a sample made of the optimized process was confirmed through an optical analysis, particle size analysis, and potentiometric analysis. The SPF in-vitro value of the cosmetics samples containing hybrid titanium dioxide showed 15~30% higher levels than the cosmetics samples containing both titanium dioxides mixture.

Strength property improvement of OCC-based paper by chemical and mechanical treatments (2) (골판지 고지의 물리화학적 처리에 의한 강도향상 (제2보))

  • Lee, Jong-Hoon;Seo, Yung B.;Jeon, Yang;Lee, Hak-Lae;Shin, Jong-Ho
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
    • /
    • 2000.04a
    • /
    • pp.69-69
    • /
    • 2000
  • In the previous experiment, it was found that OCC pre-treatment with Hobat mixer at 20-25% consistency for 3 hrs or more followed by the application of the equal refining time, caused the increase of tensile strength, burst strength, compressive strength and tear resistance, compared to the no pre-treated. Four completely different fibers, which were Hw-BKP, Sw-BKP, White ledger, and OCC were selected for this experiment to investigate the effect of mechanical pre-treatment process on different fibers. From the experiment, it was found that the mechanical pre-treatment did not decrease fiber length at all, but decreased freeness, compared to the no pre-treated, when the same refining time was applied WRVs of the pre-treated fibers were higher than the no pre-treated at the same freeness level. It was speculated that the mechanical pre-treatment induced only hydrophilic nature of fibers without damaging fiber length by delaminating fiber walls. The fiber surface area and the physical strength differences of handsheets will be discussed in the next publication.

  • PDF