• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2005년도 국제학술심포지움 The 44th Annual Meeting of Korean Society for Life Science
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• pp.52-55
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• 2005

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.II
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• pp.295-299
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• 2000

We have developed bio-system derived algorithm: Leaf Cellular Automata(LCA). LCA are one form of cellular automata. LCA are reffered to activity of a leaf. LCA have four layers: the "CO$_2$ Layer", the "Stoma Layer", the "Starch Layer" and the "Water Layer". In order to evaluate this optimization algorithm, we used a pattern matching problem.

• Asian-Australasian Journal of Animal Sciences
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• 제28권3호
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• pp.420-427
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• 2015

It is necessary to understand the cellular uptake and cytotoxicity of food-grade delivery systems, such as ${\beta}$-lactoglobulin (${\beta}$-lg) nanoparticles, for the application of bioactive compounds to functional foods. The objectives of this study were to investigate the relationships between the physicochemical properties of ${\beta}$-lg nanoparticles, such as particle size and zeta-potential value, and their cellular uptakes and cytotoxicity in Caco-2 cells. Physicochemical properties of ${\beta}$-lg nanoparticles were evaluated using particle size analyzer. Flow cytometry and confocal laser scanning microscopy were used to investigate cellular uptake and cytotoxicity of ${\beta}$-lg nanoparticles. The ${\beta}$-lg nanoparticles with various particle sizes (98 to 192 nm) and zeta-potential values (-14.8 to -17.6 mV) were successfully formed. A decrease in heating temperature from $70^{\circ}C$ to $60^{\circ}C$ resulted in a decrease in the particle size and an increase in the zeta-potential value of ${\beta}$-lg nanoparticles. Non-cytotoxicity was observed in Caco-2 cells treated with ${\beta}$-lg nanoparticles. There was an increase in cellular uptake of ${\beta}$-lg nanoparticles with a decrease in particle size and an increase in zeta-potential value. Cellular uptake ${\beta}$-lg nanoparticles was negatively correlated with particle size and positively correlated with zeta-potential value. Therefore, these results suggest that the particle size and zeta-potential value of ${\beta}$-lg nanoparticles play an important role in the cellular uptake. The ${\beta}$-lg nanoparticles can be used as a delivery system in foods due to its high cellular uptake and non-cytotoxicity.

• Molecular & Cellular Toxicology
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• 제3권sup4호
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• pp.34.1-34.1
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• 2007

• 대한수의학회지
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• 제24권2호
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• pp.169-171
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• 1984

An expeliment was carried out to measure the cellular immune response in guinea pigs by sensitizing the animals with 2, 4-dinitrochlorobenzene(DNCB). The guinea pigs could be sensitized with one application of DNCB. The sensitizing and challenge dose was standardized. The histological response was characteristic of a delayed hypersensitivity reaction.

• Journal of Ginseng Research
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• 제45권2호
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• pp.228-235
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• 2021

Backgroud: Ginsenoside compound K (GK) is a major metabolite of protopanaxadiol-type ginsenosides and has remarkable anticancer activities in vitro and in vivo. This work used an ionic cross-linking method to entrap GK within O-carboxymethyl chitosan (OCMC) nanoparticles (Nps) to form GK-loaded OCMC Nps (GK-OCMC Nps), which enhance the aqueous solubility and stability of GK. Methods: The GK-OCMC Nps were characterized using several physicochemical techniques, including x-ray diffraction, transmission electron microscopy, zeta potential analysis, and particle size analysis via dynamic light scattering. GK was released from GK-OCMC Nps and was conducted using the dialysis bag diffusion method. The effects of GK and GK-OCMC Nps on PC3 cell viability were measured by using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Fluorescent technology based on Cy5.5-labeled probes was used to explore the cellular uptake of GK-OCMC Nps. Results: The GK-OCMC NPs had a suitable particle size and zeta potential; they were spherical with good dispersion. In vitro drug release from GK-OCMC NPs was pH dependent. Moreover, the in vitro cytotoxicity study and cellular uptake assays indicated that the GK-OCMC Nps significantly enhanced the cytotoxicity and cellular uptake of GK toward the PC3 cells. GK-OCMC Nps also significantly promoted the activities of both caspase-3 and caspase-9. Conclusion: GK-OCMC Nps are potential nanocarriers for delivering hydrophobic drugs, thereby enhancing water solubility and permeability and improving the antiproliferative effects of GK.

• Molecules and Cells
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• 제39권3호
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• pp.179-185
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• 2016

Posttranscriptional regulation of RNA metabolism, including RNA processing, intron splicing, editing, RNA export, and decay, is increasingly regarded as an essential step for fine-tuning the regulation of gene expression in eukaryotes. RNA-binding proteins (RBPs) are central regulatory factors controlling posttranscriptional RNA metabolism during plant growth, development, and stress responses. Although functional roles of diverse RBPs in living organisms have been determined during the last decades, our understanding of the functional roles of RBPs in plants is lagging far behind our understanding of those in other organisms, including animals, bacteria, and viruses. However, recent functional analysis of multiple RBP family members involved in plant RNA metabolism and elucidation of the mechanistic roles of RBPs shed light on the cellular roles of diverse RBPs in growth, development, and stress responses of plants. In this review, we will discuss recent studies demonstrating the emerging roles of multiple RBP family members that play essential roles in RNA metabolism during plant growth, development, and stress responses.

• Molecules and Cells
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• 제20권2호
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• pp.303-303
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• 2005

• 한국지리정보학회지
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• 제21권4호
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• pp.202-217
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• 2018

인간 활동에 의한 도시의 토지이용변화는 도시의 공간구조와 생태계에 영향을 미친다. 토지이용변화 패턴을 파악하고 미래의 토지이용변화를 모의하는 것은 지속가능한 도시 관리를 위해 필요하지만, 토지이용변화의 불확실성과 복잡성으로 인해서 이를 효과적으로 모의하기 어렵다. 셀룰러오토마타 모형은 도시토지이용변화에 널리 사용되는 모형으로, 격자기반의 변화를 모의한다. 하지만, 격자기반의 모의는 실제 토지이용변화 특성을 반영하기 어렵고, 토지이용의 파편화가 나타나는 한계가 있다. 이러한 문제를 보완하기 위해 제작된 객체기반 셀룰러오토마타모형은 토지패치 객체별 변화를 모의한다. 본 연구는 하남시를 대상으로 객체기반 토지이용변화 모형을 제작하여 미래의 토지이용변화를 모의하였다. 제작된 모형의 정확도를 평가하는 성능지수는 24.1%로 평가되었다. 기준시나리오를 적용한 2038년의 토지이용변화 모의결과, 시가지는 16.4% 감소한 반면, 농경지는 9.0% 증가하였고, 초지는 19.3% 증가하였다. 개발시나리오의 경우 시가지는 22.4% 증가하였고, 농경지는 26.1% 감소한 반면, 산림과 초지는 큰 변화가 나타나지 않았다. 보전시나리오의 경우 시가지는 29.5%, 농경지는 8.8%감소하였고, 산림과 초지는 각각 6%, 42.8% 증가하였다. 본 모형은 실제 도시 토지이용변화를 효과적으로 모의하여 공간계획을 위한 의사결정지원도구로 사용될 수 있다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 춘계학술대회
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• pp.142-142
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• 2020