• Journal of the Korean Crystal Growth and Crystal Technology
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• v.15 no.6
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• pp.260-262
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• 2005

Thickness optimization of heavily doped p-type seeding layer was studied to improve performance of thin film silicon solar cell. We used liquid phase epitaxy (LPE) to grow active layer of $25{\mu}m$ thickness on $p^+$ seeding layer. The cells with $p^+$ seeding layer of $10{\mu}m\;to\;50{\mu}m$ thickness were fabricated. The highest efficiency of a cell is 12.95%, with $V_{oc}=633mV,\;J_{sc}=26.5mA/cm^2$, FF = 77.15%. The $p^+$ seeding layer of the cell is $20{\mu}m$ thick. As thicker seeding layer than $20{\mu}m$, the performance of the cell was degraded. The results demonstrate that the part of the recombination current is due to the heavily doped seeding layer. Thickness of heavily doped p-type seeding layer was optimized to $20{\mu}m$. The performance of solar cell is expected to improve with the incorporation of light trapping as texturing and AR coating.

• 한국신재생에너지학회:학술대회논문집
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• 2005.06a
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• pp.168-171
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• 2005

Thickness optimization of heavily doped p-type seeding layer was studied to improve performance of thin film silicon solar cell. We used liquid phase epitaxy (LPE) to grow active layer of $25{\MU}m$ thickness on p+ seeding layer. The cells with p+ seeding layer of $10{\mu}m\;to\;50{\mu}m$ thickness were fabricated. The highest efficiency of a cell is $12.95\%$, with Voc=633mV, $Jsc=26.5mA/cm^2,\;FF=77.15\%$. The $P^+$ seeding layer of the cell is $20{\mu}m$, thick. As thicker seeding layer than $20{\mu}m$, the performance of the cell was degraded. The results demonstrate that the part of the recombination current is due to the heavily doped seeding layer. Thickness of heavily doped p-type seeding layer was optimized to $20{\mu}m$. The performance of solar cell is expected to improve with the incorporation of light trapping as texturing and AR coating.

• Investigative Magnetic Resonance Imaging
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• v.21 no.2
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• pp.119-124
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• 2017

Chromophobe renal cell carcinoma (RCC) is an uncommon subtype of RCC having a better prognosis than clear cell RCC. Although there are several reports of seeding metastasis of RCC after biopsy, seeding metastasis of chromophobe RCC after surgical resection has seldom been reported. Here, we describe a case of multiple seeding metastases in the abdomen and pelvis 78 months after robot-assisted laparoscopic partial nephrectomy, without prior history of biopsy for chromophobe RCC in the right kidney. As magnetic resonance imaging (MRI) of the pelvic mass showed a similar appearance to the primary renal mass and displayed separate margins with the rectum and prostate gland, we were able to make a diagnosis before pathologic confirmation.

• Journal of Bio-Environment Control
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• v.16 no.1
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• pp.62-66
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• 2007

This study was carried out to develop the stable plug seedling production for hydroponics of spinaches by various nursery media, plug tray size and seed number per plug tray cell. When plant grown in various nursery media, the seeding stand rate was shown in order of granular rockwool with good water retention, granular rockwool>granular rockwool mixed with pearlite>cocopeat>pearlite>poly urethane foame. Thus, poly urethane foame indicated the lower seedling stand rate. There was no difference in growth of the seedlings md the seeding stand rate by the plug tray size, and no significant difference in the plant height and the number of leaves among the seed number per plug tray cell. But, leaf area of plant in 2 grains seeding per cell was $113.0cm^2$, was wider in compared with 5 grains seeding of which leaf area was $88.0cm^2$. Accordingly, the leaf area per plant decreased as more and more the number of seeds per plug tray cell increased. The fresh weight of a plant per plug tray cell was the heaviest with 12.5g in the 2 grains, and the total fresh weight of plants per cell was 33.9g in 4 grains seeding, thus it tended to was bigger compared with other treatments. Consequently, given that the number of seeds per cell was decreased, the fresh weight of a plant increased. On the other hand, the total fresh weight per cell showed a tendency to be reducing as more and more the number of seeds per plug tray cell decreased. The yield in the 4 grains seeding was increased by 46% as $14,910kg{\cdot}ha^{-1}$ in compared with the yield in 2 grains seeding as $10,200kg{\cdot}ha^{-1}$.

• Journal of Chest Surgery
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• v.26 no.2
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• pp.80-85
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• 1993

Synthetic and biosynthetic vascular grafts of small diameter have long been considered to be prone to thrombosis, ultimately leading to the complete graft occlusion. Endothelial cell seeding onto synthetic blood-contacting surfaces has been suggested to be an ideal means to solve this problem. This study described a culture method of bovine endothelial cells and evaluated blood-compatibility and seeding efficiency of cultured endothelial cells. Bovine pulmonary artery endothelial cells were harvested enzymatically and grown to confluence on polystyrene culture flask surfaces using established techniques. The identification of endothelial cells was made through the demonstration of expression of factor VIII R:Ag by immunofluorescent technique. To quantitate the effect of improvement in blood-compatibility of viable endothelial cells, endothelial monolayers were exposed to blood containing $\^$111/In-oxine labeled platelets. Viable endothelial monolayers retained less labeled platelets than control surfaces. The Indium-labeled endothelial cells were seeded onto three different blood-contacting surfaces of Dacron vascular graft immobilized in specially equipped wells and incubated for specific time intervals (t=15, 30, 60, 120 minutes). Longer incubation times showed improved cell adherence in collagen-coated and fibrin-coated Dacron vascular graft groups. However in untreated Dacron grafts, no direct relationship was observed between incubation time and endothelial cell seeding efficiency. This may be due to leakage of endothelial cells through porosity of Dacron grafts in this in-vitro experimental condition.

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.707-714
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• 2004

How to improve the cell culture method on scaffolds is important in the tissue engineering fileld. In this study, we optimized seeding and culture methods to vascular smooth muscle cells (VSMCs) on biodegradable polymer scaffold. The primary culture of VSMCs obtained from canine external jugular vein was accomplished by applying the explant-derived method. The primary cultured VSMCs were seeded into scaffolds and then cultured by using various different methods; static or dynamic seeding, static or dynamic culture. The difference in proliferative response of VSMCs was analyzed with an alamar blue assay. Cell-polymer construct was examined by histochemical method and scanning electron microscopy. Mesh type scaffold ($10 \times 10 \times0.4 mm$) was made of polyglycolic acid (PGA) suture thread. The PGA mesh type scaffold was 45% in porosity, and 0.03 g in weight. The primary cultured VSMCs were confirmed with immunohistochemical staining using monoclonal anti-$\alpha$-smooth muscle actin. The density and distribution of proliferated VSMCs within the scaffold and cellular adherence on the surface of the scaffold showed better results in the static seeding condition than in the dynamic condition. Under the same condition of seeding method as the static condition, the dynamic culture condition showed enhanced proliferation rates of the VSMCs when compared to the static culture condition. In conclusion, to improve the VSMCs proliferation in vitro, static seeding is better than the dynamic condition. In the culture condition, however, culture under the dynamic status is better than the static condition. This was a pilot study to manufacture artificial vascular vessel by tissue engineering.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.4
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• pp.407-415
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• 2009

To investigate the effects of seeding date, nursery days and plug cell size on seedling growth and quality of waxy corn. This study was carried out in the field of the National Institute of Crop Science in 2007. Varieties used were two waxy corn hybrids cvs. Chalok# 1 and Chalok# 4. The plant height, number of leaves and dry weight of waxy corn seedlings were significantly affected by seeding dates and plug cell size, while no differences between varieties. The growth of seedlings was faster in March 14 seeding than in May 8 and July 4 seedings. Based on plant height, March 14 seeding, May 8 seeding and July 3 seeding reached suitable size for transplanting after 30 days (21.6cm), 15 days (25.5~26.9cm) and 10 days (21.6~24.0cm) in the nursery, respectively. Seedlings of March 14 seedling and May 8 and July 3 seeding reached suitable number of leaves i,e. 2~3 leaves, 15~20 days after seeding on March 14 and 10 days after seeding on May 8 and July 3. Dry weights of 30 days seedling for March 14 seeding, 20 days seedling for May 8 seeding and 15 days seedling for July 3 seeding were 0.43g, 0.57~0.67g and 0.53g, respectively, and the dry weight of seedlings increased by 2~3 folds at the later nursery days. The suitable nursery days of waxy corn were 20 days on March 14 seeding and 10~15 days on May 8 or July 3 seeding.

• Journal of Biomedical Engineering Research
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• v.16 no.1
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• pp.1-8
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• 1995

A variety of experiments of endothelial cell seeding onto artificial vessels have been performed. To improve endothelialization, one or two components of the extracellular matrix (ECM) have been used as an underlying matrix. In this study, the whole ECM excreted from fibroblasts was used as an underlying matrix. Fetal human fibroblasts were cultured on a polyurethane (PU) sheet. After a conflu; ence was attained, the cytoskeleton and the nuclei of the fibroblast were destroyed using Triton-X. Mitomycin, or irradiation. Omental microvascular endothelial cells from adult human were seeded onto various substrates. After 12 days in culture, the cells were counted. It was observed that the ECM treated by irradiation had the highest cell number. In addition, the cells on this substrate exhibited the most typical endothelial cell morphology. For preliminary animal experiments the PU vessels (inner diameter, 1.5mm) coated with ECM were implanted in the infrarena] abdominal aorta of rat. After the vessels had been implanted for 5 weeks, it was found that the surface of the PU vessels was completely covered with endothelia] cells. In conclusion, we can state that the fibroblast-derived whole ECM makes a better underlying substrate for the endothelialization of small diameter artificial vessels.

• Journal of Biomedical Engineering Research
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• v.11 no.1
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• pp.131-140
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• 1990

To improve antithrombogenicity of polymer that used in vascular graft and artificial organs, seeding of human endothelial cells on the polyurethane was studied. Human endothelial cells were ismlated from human umbilical veins, using type I collagenase, and identified with goat anti vWF antibodies. Human endothilial cell seeding was tried upon the polyurethane which has good mechanical property and resists stresses. The hydrophobic polyurethane surface was changed hydrophilic by corona discharf:e treatment. Surface hydrophilicity was measured with Wilhemly plate method and the goniometer. To evaluate matrix protein adsorption, fibronectin adsorption test was done. To eveluate cell adhesion, human endothelial cell attachment forces were measured rising a perfusion chamber of , ism diamter. Less cells were detached from the hydrophilically treated polyurethane. This showed that corona discharge on the polyurethane could improve matrix adsorption and endothelial cell attachment.

• Korean Journal of Plant Resources
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• v.35 no.1
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• pp.29-35
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• 2022

Elsholtzia byeonsanensis is a Korean native plant of the Lamiaceae family, and was first introduced in the Korean Journal of Plant Taxonomy. This plant has a short plant length of 20-35 cm, with leathery and glossy leaves. The purple flowers bloom in November, so they are highly useful as potted or ground cover ornamental plants. In this study, tray cell size (128, 162, and 200-cell trays), soil composition [horticultural soil (HS): decomposed granite (DG) 1:1, 1:2, and 1:4 (v:v)], seeding rate (1, 2, and 3 seeds per cell) and additional fertilizer concentrations (0, 250, 500, and 1000 mg/L Hyponex) were tested to find out the optimum seedling growth conditions. As a result of the study, E. byeonsanensis growth increased proportionally as the cell size of the tray increased. The growth was inhibited as the percentage of DG increased. As for the growth according to the seeding rate, plant height, plant width, leaf length, and leaf width decreased as the seeding rate increased, but there was no difference in the number of leaves, stem diameter, and root length. There was an increasing trend in seedling growth as the additional fertilizer concentration increased. Therefore, for producing E. byeonsanensis seedling, it is most effective to fill HS or HS:DG 1:1 in 128-cell trays, sow one seed per cell, and spray the seedling at 1000 mg/L for additional fertilizer.