• 한국기계가공학회지
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• 제19권2호
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• pp.111-118
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• 2020

Recently, bioenergy research using microalgae, one of the most promising biofuel sources, has attracted much attention. Cell disruption, which can be classified as physical or chemical, is essential to extract functional ingredients from microalgae. In this study, we investigated the cell disruption efficiency of Chlorella sp. using low-frequency non-focused ultrasound (LFNFU). This is a continuously physical method that is superior to chemical methods with respect to environmental friendliness and low processing cost. A flat panel photobioreactor was employed to cultivate Chlorella sp. and its growth curve was fitted both with Logistic and Gompertz models. The temporal change in cell reduction by cell disruption using LFNFU was fitted with a Logistic model. The experimental conditions that were investigated were the initial concentration of microalgal cells, relative amplitude of output ultrasound waves, processing volume of microalgal cells, and initial pH value. The optimal conditions for the most efficient cell disruption were determined through the various tests.

• 한국멀티미디어학회논문지
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• 제11권12호
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• pp.1635-1648
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• 2008

The extraction of important features in cancer cell image analysis is a key process in grading renal cell carcinoma. In this study, we applied three-dimensional (3D) texture feature extraction methods to cell nuclei images and evaluated the validity of them for computerized cell nuclei grading. Individual images of 2,423 cell nuclei were extracted from 80 renal cell carcinomas (RCCs) using confocal laser scanning microscopy (CLSM). First, we applied the 3D texture mapping method to render the volume of entire tissue sections. Then, we determined the chromatin texture quantitatively by calculating 3D gray-level co-occurrence matrices (3D GLCM) and 3D run length matrices (3D GLRLM). Finally, to demonstrate the suitability of 3D texture features for grading, we performed a discriminant analysis. In addition, we conducted a principal component analysis to obtain optimized texture features. Automatic grading of cell nuclei using 3D texture features had an accuracy of 78.30%. Combining 3D textural and 3D morphological features improved the accuracy to 82.19%. As a comparative study, we also performed a stepwise feature selection. Using the 4 optimized features, we could obtain more improved accuracy of 84.32%. Three dimensional texture features have potential for use as fundamental elements in developing a new nuclear grading system with accurate diagnosis and predicting prognosis.

• Journal of Microbiology and Biotechnology
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• 제31권3호
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• pp.349-357
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• 2021

Monoclonal antibodies are widely used as diagnostic reagents and for therapeutic purposes, and their demand is increasing extensively. To produce these proteins in sufficient quantities for commercial use, it is necessary to raise the output by scaling up the production processes. This review describes recent trends in high-density cell culture systems established for monoclonal antibody production that are excellent methods to scale up from the lab-scale cell culture. Among the reactors, hollow fiber bioreactors contribute to a major part of high-density cell culture as they can provide a tremendous amount of surface area in a small volume for cell growth. As an alternative to hollow fiber reactors, a novel disposable bioreactor has been developed, which consists of a polymer-based supermacroporous material, cryogel, as a matrix for cell growth. Packed bed systems and disposable wave bioreactors have also been introduced for high cell density culture. These developments in high-density cell culture systems have led to the monoclonal antibody production in an economically favourable manner and made monoclonal antibodies one of the dominant therapeutic and diagnostic proteins in biopharmaceutical industry.

• Applied Microscopy
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• 제50권
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• pp.6.1-6.6
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• 2020

The cerebellum is a region of the brain that plays an important role in motor control. It is classified phylogenetically into archicerebellum, paleocerebellum and neocerebellum. The Purkinje cells are lined in a row called Purkinje cell layer and it has a unique dendritic branches with many spines. The previous study reported that there is a difference of synapse density according to the lobules based on large two-dimensional data. However, recent study with high voltage electron microscopy showed there was no differences in dendritic spine density of the Purkinje cell according to its phylogenetic lobule. We analyzed Purkinje cell density in the II, VI and X lobules by stereological modules and synaptic density was estimated by double disector based on Purkinje cell density in the molecular layer of each lobule. The results showed that there was significant difference in the Purkinje cell density and synapse number according to their phylogenetic lobules. The number of Purkinje cell in a given volume was larger in the archicerebellum, but synapse density was higher in the neocerebellum. These data suggest that cellular and synaptic organization of the Purkinje cell is different according to their phylogenetic background.

• 대한기계학회논문집B
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• 제33권6호
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• pp.443-453
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• 2009

The Rayleigh-Taylor instability, the bubble rising in both partially and fully filled containers and the droplet splash are simulated by an in-house solution code(PowerCFD), which are typical benchmark problems among multiphase flows with material interface due to density difference. The present method(code) employs an unstructured cell-centered method based on a conservative pressure-based finite-volume method with interface capturing method(CICSAM) in a volume of fluid(VOF) scheme for phase interface capturing. The present results are compared with other numerical solutions found in the literature. It is found that the present method simulates efficiently and accurately complex free surface flows such as multiphase flows with material interface due to both density difference and instability.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1995년도 추계학술발표회논문집(1)
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• pp.480-486
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• 1995

Turbulent flow field in a subchannel of bare rod bundles has been numerically simulated using the control volume based finite element method. Launder & Ying model of Reynolds stress and Lam & Bremhorst low-Reynolds number model are implemented in k-$\varepsilon$ equations and momentum equations. Secondary flows are simulated using the stream function and vorticity approach. The control volume based finite element method enable to use the upwind scheme (donor cell scheme). Sensitivity of the constants in the models are studied, and proper values are found to get the close result to the measured flow distributions.

• 대한기계학회논문집
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• 제17권2호
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• pp.445-451
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• 1993

For the calculation of transonic laminar flow fields in cascades of turbomachinery, a finite volume method employing Jameson's Runge-Kutta integration scheme as a basic algorithm is presented. The cell-vertex scheme introducing half-spacing mesh cells is developed. For the velocity gradients in the stress terms the integration with divergence theorem is used for the average concept. Some numerical results show good agreement with experimental data.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2011년 춘계학술대회논문집
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• pp.2-5
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• 2011

This paper presents a numerical study on multiphase flows induced by wall adhesion The CSF(Continuum Surface Force} model is used for the calculation of the surface tension force and implemented in an in-house solution code(PowerCFD). The present method(code) employs an unstructured cell-centered method based on a conservative pressure-based finite-volume method with volume capturing method(CICSAM) in a volume of fluid(VOF) scheme for phase interface capturing As an application of the present method, the effects of wall adhesion are numerically simulated with the CSF model for a shallow pool of water located at the bottom of a cylindrical tank. Two different cases are computed, one in which the water wets the wall and one in which the water does not wet the wall. It is found that the present method simulates efficiently and accurately surface tension-dominant multiphase flows induced by wall adhesion.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2008년도 춘계학술대회논문집
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• pp.572-575
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• 2008

Two-dimensional multiphase flows due to density difference such as the Rayleigh-Taylor instability problem and the droplet splash are simulated by an in-house solution code(PowerCFD). This code employs an unstructured cell-centered method based on a conservative pressure-based finite-volume method with interface capturing method in a volume of fluid(VOF) scheme for phase interface capturing. The present results are compared with other numerical solutions found in the literature. It is found that the present code simulates complex free surface flows such as multiphase flows due to density difference efficiently and accurately.

• 소성∙가공
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• 제10권5호
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• pp.396-401
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• 2001

The concept of the mixtures can be used not only in the composites but also in the materials with precipitates and matrix. In this investigation, the finite element method of axisymmetric unit cell models and the rule of mixtures of the Voigt and the Reuss models are used to analyze the overall mechanical response of composites with homogeneously distributed particles. The calculations have been cameo out by taking the materials as i) hardening and ii) perfect plastic materials. The Plastic properties are predicted for various volume fractions of the soft and hard particles. The computational results are compared with the results of the rule of mixtures. It is found that the plastic flow curves agree well with the Voigt model when the volume fraction of the particles is high. On the other hand, the calculated flow curves exist between the Voigt model and the Reuss model when the volume fraction of the particles is low.