• Title/Summary/Keyword: cell recovery

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Neuroprotective Effect of Ginseng radix on ICH-induced Rats

  • Jang, Kwan-Ho;Song, Yun-Kyung;Lim, Hyung-Ho
    • The Journal of Korean Medicine
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    • v.26 no.4
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    • pp.87-97
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    • 2005
  • Backgrounds: Intracerebral hemon-hage is one of the most devastating types of stroke. Ginseng radix, the root of Panax Ginseng, C. A. MEYER (Araliaceae), is one of the most famous medicinal herbs with various therapeutic applications. Objectives: In the present study, the effect of aqueous extract of Ginseng radix on intracerebral hemorrhage-induced neuronal cell death in rats was investigated. Materials and Methods: Step-down avoidance task, Nissl staining, immunohistochemistry for caspase-3 and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay were used for this study. Results: The present results show that hemorrhage-induced lesion volume and apoptotic neuronal cell death in the striatum were significantly suppressed by treatment with Ginseng radix, resulting in enhancement of short-ten-n memory. Conclusions: We have shown that Ginseng radix has a neuroprotective effect on stroke, and aids the recovery from central nervous system sequelae following stroke.

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Moce of Action of the Purified Cell Wall Lytic Enzyme from Bacillus sp. (Bacillus sp.로부터 분리 정제한 Cell Wall 분해효소의 반응특성)

  • Kim, Tae-Ho;Shin, Woo-Chang;Lee, Dong-Sun;Hong, Soon-Duck
    • Microbiology and Biotechnology Letters
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    • v.23 no.6
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    • pp.671-677
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    • 1995
  • An extracellular enzyme showing lytic activity on E. coli peptidoglycan had been isolated from Bacillus sp. BL-29. The lytic enzyme was purified to homogeneity by ion-exchange chromatography and gel filtration, with a recovery of 5%. The enzyme was monomeric and had an estimated molecular weight of 31,000 Da. The mode of action of the purified enzyme was also investigated. When the purified lytic enzyme was incubated with cell wall peptidoglycan, N-terminal amino groups were released without the release of reducing groups. The N-terminal amino acid released was identified as dinitrophenylalanine (DNP-alanine) by analysis of terminal amino acid by dinitrophenylation method. This result suggests that the lytic enzyme should be a kind of N-acetylmura-myl-L-alanine amidase.

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Emulsion rheology and properties of polymerized high internal phase emulsions

  • Lee, Seong-Jae
    • Korea-Australia Rheology Journal
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    • v.18 no.4
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    • pp.183-189
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    • 2006
  • High internal phase emulsions are highly concentrated emulsion systems consisting of a large volume of dispersed phase above 0.74. The rheological properties of high internal phase water-in-oil emulsions were measured conducting steady shear, oscillatory shear and creep/recovery experiments. It was found that the yield stress is inversely proportional to the drop size with the exponent of values between 1 and 2. Since the oil phase contains monomeric species, microcellular foams can easily be prepared from high internal phase emulsions. In this study, the microcellular foams combining a couple of thickeners into the conventional formulation of styrene and water system were investigated to understand the effect of viscosity ratio on cell size. Cell size variation on thickener concentration could be explained by a dimensional analysis between the capillary number and the viscosity ratio. Compression properties of foam are important end use properties in many practical applications. Crush strength and Young's modulus of microcellular foams polymerized from high internal phase emulsions were measured and compared from compression tests. Of the foams tested in this study, the foam prepared from the organoclay having reactive group as an oil phase thickener showed outstanding compression properties.

Buffer-Optimized High Gradient Magnetic Separation: Target Cell Capture Efficiency is Predicted by Linear Bead-Capture Theory

  • Waseem, Shahid;Udomsangpetch, Rachanee;Bhakdi, Sebastian C.
    • Journal of Magnetics
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    • v.21 no.1
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    • pp.125-132
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    • 2016
  • High gradient magnetic separation (HGMS) is the most commonly used magnetic cell separation technique in biomedical science. However, parameters determining target cell capture efficiencies in HGMS are still not well understood. This limitation leads to loss of information and resources. The present study develops a bead-capture theory to predict capture efficiencies in HGMS. The theory is tested with CD3- and CD14-positive cells in combination with paramagnetic beads of different sizes and a generic immunomagnetic separation system. Data depict a linear relationship between normalized capture efficiency and the bead concentration. In addition, it is shown that key biological functions of target cells are not affected for all bead sizes and concentrations used. In summary, linear bead-capture theory predicts capture efficiency ($E_t$) in a highly significant manner.

Improvement of the electro-transformed cell yield for Bifidobacterium sp. with large DNA

  • Park, Min Ju;Park, Myeong Soo;Ji, Geun Eog
    • Korean Journal of Food Science and Technology
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    • v.51 no.2
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    • pp.188-191
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    • 2019
  • In this study, the poor electro-transformant yield of large DNA in bifidobacteria was improved by increasing the DNA concentration, which was amplified by enhancing electroporation conditions: treating the cell wall weakening agent and cell membrane permeabilizing molecule as well as changing the electrical parameter. In the enhanced conditions, the electroporation frequency increased from 15 to 71 times compared to the initial conditions at the same DNA concentration. As the DNA concentration increased, the difference in the electroporation frequency between the two conditions became greater, and the curve of the enhanced conditions seemed to be saturated with a DNA concentration over $4{\mu}g$. The present study provided a clue to the recovery of the electroporation frequency with large DNA and formulated the relationship between the DNA concentration, the DNA size and the electroporation frequency in bifidobacteria. Therefore, this study will contribute to the expansion of molecular genetic studies of bifidobacteria.

Adaptive Video Watermarking Using Half-cell Motion Vector (반화소 움직임 벡터를 이용한 적응적 비디오워터마킹)

  • Shinn Brian-B.;Kim Min-Yeong;D Khongorzul;Lee In-Sung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.7
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    • pp.1214-1221
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    • 2006
  • Header compression scheme is suggested as a solution to reduce the inefficient overhead of general packet stream data. Especially, it is shown that there are more overhead rate for real-time media stream links such as voice because of its short payload size, and it is possible to get higher bandwidth efficiency using the header compression scheme. There are two kinds of error recovery in header compression such as Periodic Header Refresh(PHR) and Header Request(HR) schemes. In this paper, we analyze the performance of these two compression recovery schemes, and some results such as the overhead rate, bandwidth gain and bandwidth efficiency(BE) are presented.

A Study on Improvement of Valuable Metals Leaching and Distribution Characteristics on Waste PCBs(Printed Circuit Boards) by Using Pulverization Process (폐 PCBs의 미분쇄 공정 적용에 따른 유가금속 분포 특성 및 금속 침출 향상에 관한 연구)

  • Han, Young-Rip;Choi, Young-Ik
    • Journal of Environmental Science International
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    • v.24 no.2
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    • pp.245-251
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    • 2015
  • The main objective of this study is to recovery valuable metals with metal particle size distributions in waste cell phone PCBs(Printed Circuit Boards) by means of pulverization and nitric acid process. The particle size classifier also was evaluated by specific metal contents. The PCBs were pulverized by a fine pulverizer. The particle sizes were classified by 5 different sizes which were PcS1(0.2 mm below), PcS2(0.20~0.51 mm), PcS3(0.51~1.09 mm), PcS4(1.09~2.00 mm) and PcS5(2.00 mm above). Non-magnetic metals in the grinding particles were separated by a hand magnetic. And then, Cu, Co and Ni were separated by 3M nitric acid. Particle diameter of PCBs were 0.388~0.402 mm after the fine pulverizer. The sorting coefficient were 0.403~0.481. The highest metal content in PcS1. And the bigger particle diameter, the lower the valuable metals exist. The recovery rate of the valuable metals increases in smaller particle diameter with same leaching conditions. For further work, it could improve to recovery of the valuable metals effectively by means of individual treatment, multistage leaching and different leaching solvents.

Optimising the Extraction of Bacteria, Heterotrophic Protists and Diatoms, and Estimating Their Abundance and Biomass from Intertidal Sandy Sediments

  • Lee, Won-Je;Patterson, David J.
    • Journal of the korean society of oceanography
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    • v.37 no.2
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    • pp.58-65
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    • 2002
  • The study of microbial communities in sediments is impaired by the lack of reliable extraction methods. This study reports on the efficiency of recovery of a method for extracting bacteria, protists and diatoms from sandy sediments using a modified decant/fix method. The best extractions were achieved after fixation with a microtubule stabilising fixative and subsequent sonication for 80 seconds. We estimate that the efficiencies of recovery of bacteria and mixed heterotrophic flagellates were $96.4{\pm}3.5\%$ and $96.9{\pm}4.6\%$, respectively. Diatoms were recovered with an efficiency of 38-83% and varied considerably from species to species. This study suggests that the decant/fix method is effective in extracting small cells such as bacteria and heterotrophic flagellates, and that the efficiency of recovery of the method varies due to cell length and different types of organisms. When microbial carbon biomass had been underestimated by up to 32%, with much of that relating to larger cells such as microalgae and ciliates. We note that the corrected abundances may be still a subset of the total numbers present.

A study on the recovery of discoloration of Pyropia yezoensis in laboratory culture (실내배양에서 방사무늬김의 황백화 회복에 대한 연구)

  • Lee, Sang Yong;Kim, Young Hee;Kim, Soo Hong;Yoo, Hyun Il
    • Korean Journal of Environmental Biology
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    • v.37 no.4
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    • pp.719-725
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    • 2019
  • To recover the discoloration of Pyropia yezoensis, additional NO3- and PO43- nutrients were supplied to the discolored blades. As a result, the efficiency of photosynthesis was increased from two days after incubation with NO3- and five days after incubation with PO43-. In addition, the chromaticity analysis results showed significant changes. Furthermore, the cell vacuoles bloated due to discoloration were reduced again when they were restored from discoloration, suggesting that the control of discoloration is feasible by nutrient supply.

Smart Honeycomb Sandwich Panels With Damage Detection and Shape Recovery Functions

  • Okabe, Yoji;Minakuchi, Shu;Shiraishi, Nobuo;Murakami, Ken;Takeda, Nobuo
    • Advanced Composite Materials
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    • v.17 no.1
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    • pp.41-56
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    • 2008
  • In this research, optical fiber sensors and shape memory alloys (SMA) were incorporated into sandwich panels for development of a smart honeycomb sandwich structure with damage detection and shape recovery functions. First, small-diameter fiber Bragg grating (FBG) sensors were embedded in the adhesive layer between a CFRP face-sheet and an aluminum honeycomb core. From the change in the reflection spectrum of the FBG sensors, the debonding between the face-sheet and the core and the deformation of the face-sheet due to impact loading could be well detected. Then, the authors developed the SMA honeycomb core and bonded CFRP face-sheets to the core. When an impact load was applied to the panel, the cell walls of the core were buckled and the face-sheet was bent. However, after the panel was heated over the reverse transformation finish temperature of the SMA, the core buckling disappeared and the deflection of the face-sheet was relieved. Hence the bending stiffness of the panel could be recovered.