• Title/Summary/Keyword: cell production

Search Result 8,058, Processing Time 0.035 seconds

The Eueet of Ginseng Extract on Physiology of Saccharomuces cereuisiae (인삼추출물이 Saccharomyces cerevisiae의 생리에 미치는 영향)

  • 주현규;이교철
    • Journal of Ginseng Research
    • /
    • v.3 no.2
    • /
    • pp.95-104
    • /
    • 1979
  • The effects of ginseng extracts on carbon dioxide generation, alcohol fermentation, and yeast cell production by Saccharomyces cerevisiae were investigated. The results are as follows. 1) In the process of fermentation, CO2 generation by yeast is faster in ginseng extracts media of 0.3%, 0. l% than in control. As the concentration of the extracts increases by 0.7% and 1.5%, CO2 generation is decreased. Among all these concentrations, CO2 generation is fastest in 0.3% of the extracts. 2) In the process of fermentation, the production of alcohol is larger in the order of 0.3%, 0.7% and 0.1% than in the control and least in 1.5%. 3) The number of yeast cell rapidly increased from 12 hours to 18 hours after cultivation and conspicuously increased in the order of 0.3%, 0.7%, 0.1%, control and 1.5%. 4) Dried yeast cell weight increased more in all the above concentration than control and among these it increased visibly in 0.3% of the extracts.

  • PDF

Status of Photovoltaics in the world (세계 태양광발전산업 현황)

  • Kang, Gi-Hwan;Yu, Gwon-Jong;Park, Kyung-Eun;Kim, Hyun-Il
    • 한국태양에너지학회:학술대회논문집
    • /
    • 2008.04a
    • /
    • pp.86-91
    • /
    • 2008
  • Amid the booming research on new and renewable energy, the photovoltaic(PV) industry has been growing around the PV advanced countries such as Japan, Germany, Europe and USA. In recent years, China became a strong performer in the world PV market share, increasing solar cell production rapidly. Both world solar cell and module production and installation rose steadily in 2007 like recent bumper years. In 2007, the PV industry produced 4.28GW and the installations reached a record high of 2.83GW, representing growth of 60percent over the previous year.

  • PDF

Role of S-Adenosylemthionine as an Intermediate in Relation between Polyamine and Ethylene Biosynthesis in Suspension-Cultured Tobacco Cells (담배 현탁배양 세포에 있어 Polyamine 과 Ethylene 생합성시 중간산물로서 S-Adenosylmethionine의 역할)

  • 박기영
    • Journal of Plant Biology
    • /
    • v.33 no.2
    • /
    • pp.87-96
    • /
    • 1990
  • The role of S-adenosylmethionine (SAM) as an intermediate in interrelation between polyamine and ethylene biosynthesis was studied in suspension cultures of Nicotiana tabacum L. Exogenous SAM stimulated the polyamine and ethylene biosynthesis in 4 day-cultured cells, which were in active cell divisions, and 10 day cultured cells, which went on with active cell elongation and senescence. SAM-induced ethylene production was more effective in 10 day-cultured cells than in 4 day-cultured cells, but SAM-induced polyamine biosynthesis was more effective in 4 day-cultured cells than in 10 day-cultured cells. Polyamine contents were increased by the blockage of ethylene biosynthetic pathway in the conversion of SAM to ethylene via 1-aminocyclopropane-1-carboxylinc acid (ACC) with aminooxyacetic acid (AOA). Also, ethylene production was increased by the inhibitors of polyamine biosynthesis such as methylglyoxal bis-(guanylhydrazone) (MGBG), dicyclohexylamine (DCHA), $\alpha$-difluoromethylarginine (DFMA) and $\alpha$-difluoromethylorinithine (DFMO). These results suggest that there may be interrelations between polyamine and ethylene biosynthesis for the competition of SAM and the inherent mechanism of switch on-off in polyamine and ethylene biosynthetic activity with the progress of cell growth and senescence.

  • PDF

Continuous Production of Sorbitol with Permeabilized Zymomonas mobilis Immobilized in Alginate and Chitin (알저네이트 및 카이틴 고정화 Zymomonas mobilis 에 의한 쏠비톨의 연속생산)

  • 최도진;김원극전억한
    • KSBB Journal
    • /
    • v.5 no.3
    • /
    • pp.223-227
    • /
    • 1990
  • This study describes the sorbitol production with permeabilized cells of Zymomonas mobilis immobilized in Ca-alginate. Toluene treated cells lose glucose-fructose oxidoreductase activity due to leaking of enzyme from the cells. In order to prevent this leakage, the permeabilized cells were immobilized in alginate and chitin. No significant loss of enzyme activity was apparent during 210h operation in a continuous process. The productivity of the continuous process was estimated to be about 3.5g/l -h for sorbitol at dilution rate $0.2h^{-1}$.

  • PDF

Improved guggulsterone production from sugars, precursors, and morphactin in cell cultures of Commiphora wightii grown in shake flasks and a bioreactor

  • Mathur, Meeta;Ramawat, K.G.
    • Plant Biotechnology Reports
    • /
    • v.2 no.2
    • /
    • pp.133-136
    • /
    • 2008
  • Cell cultures of Commiphora wightii (Arnott.) Bhandari were grown in shake flasks and a bioreactor and an increase in guggulsterone accumulation up to $18{\mu}g\;l^{-1}$ was recorded in cells grown in the production medium containing a combination of sucrose:glucose (4% total), precursors (phenylalanine, pyruvic acid, xylose, and sodium acetate), morphactin, and 2iP. A yield of $10g\;l^{-1}$ biomass and ${\sim}200{\mu}g\;l^{-1}$ guggulsterone was recorded in a 3-l flask and in a 2-l stirred tank bioreactor compared with 6.6 g biomass and $67{\mu}g\;l^{-1}$ guggulsterone in 250-ml flasks. Increased vessel size was correlated with increased biomass and guggulsterone accumulation. 2iP alone was not effective for biomass and guggulsterone accumulation in cell cultures of C. wightii.

Production of Volatile Oil Components by Cell Culture of Agastache rugosa O. Kuntze

  • Shin, Seung-Won;Kim, You-Sun;Kang, Chan-Ah
    • Natural Product Sciences
    • /
    • v.7 no.4
    • /
    • pp.120-123
    • /
    • 2001
  • To develop systems for economic production of useful essential oil compounds, callus was induced from the seedlings of Agastache rugosa and cultured on MS medium. The volatile oil fraction was extracted from the callus and investigated by mean of GC-MS. The composition of the oil was compared with that of the mother plant. As a result, sixty five compounds including ferruginol were identified in the essential oil fraction. The main component of the oil from the leaves of Agastache rugosa was methyl chavichol (53.6%). Methyl jasmonate and jasmonic acid were added to the culturing cell suspension, separately and the composition of induced oil were compared. The oils from cultured cells treated with jasmonates showed considerably different patterns. Especially, the peak of estragole was found in callus oil after treatment with methyl jasmonate as though the amount was limited to 0.58%. In general, the TIC pattern of GC-MS of the callus oil became more similar to the oil from the leaves after elicitation.

  • PDF

Prostaglandin $F_2{\alpha}$ Controls Reactive Oxygen Species in Bovine Corpus Luteum

  • Lee, Seunghyung;Yang, Boo-Keun;Park, Choon-Keun
    • Reproductive and Developmental Biology
    • /
    • v.39 no.1
    • /
    • pp.1-6
    • /
    • 2015
  • Luteolysis is a cyclical regression of the corpus luteum in many non-primate mammalian species. Prostaglandin $F_2{\alpha}$($PGF_2{\alpha}$) from the uterus and ovary induces functional and structural luteolysis in bovine. The action of $PGF_2{\alpha}$ is mediated by $PGF_2{\alpha}$ receptor located on the luteal steroidogenic and endothelial cell membranes. $PGF_2{\alpha}$ plays an important role in regulating nitric oxide production in endothelial cells of the bovine corpus luteum. Nitric oxide production and nitric oxide synthase activity are stimulated and induced by $PGF_2{\alpha}$ in luteal endothelial cells. Moreover, the reactive oxygen species inhibits progesterone secretion in bovine luteal cells and induces apoptosis. Thus, the interaction between $PGF_2{\alpha}$ and reactive oxygen species provides important aspects in physiology of the corpus luteum forfunctional and structural luteolysis.

Experimental Study of Hwanhonsan water extract on immunologic control function (환혼산전탕액(還魂散煎湯液) 면역조절반응(免疫調節反應)에 미치는 실험적(實驗的) 연구(硏究))

  • Song, Hyo-Won;Kang, Soon-So;Ryu, Do-Gon
    • The Journal of Korean Medicine
    • /
    • v.18 no.1
    • /
    • pp.446-448
    • /
    • 1997
  • This experiment was carried out to evaluate the immunological effects of Hwanhonsan extract. Hwanhonsan administration into mice enhanced Arthus reaction and DTH to sheep erythrocytes, and NK cells activities. Hwanhonsan extract augmented the DNA synthesis of mitogen-activated lymphocytes. Hwanhonsan also stimulated leucocyte migration ability, MIF and IL-2 production of T lymphocytes, but not IL-6 production of B cells. These results suggested that effect of Hwanhonsan might be chiefly due to nonspecific enhancement of NK cell activities and cell mediated immune responses.

  • PDF

Analysis on Electrical Characteristics of PV Cells considering Ambient Temperature and Irradiance Level (주변온도와 일사량을 고려한 PV Cell의 전기적 특성 분석)

  • Park, Hyeonah;Kim, Hyosung
    • The Transactions of the Korean Institute of Power Electronics
    • /
    • v.21 no.6
    • /
    • pp.481-485
    • /
    • 2016
  • When analyzing economic feasibility for installing a PV generation plant at a certain location, the prediction of possible annual power production at the site using the target PV panels should be conducted on the basis of the local weather data provided by a local weather forecasting office. In addition, the prediction of PV generating power under certain weather conditions is useful for fault diagnosis and performance evaluation of PV generation plants during actual operation. This study analyzes PV cell characteristics according to a variety of weather conditions, including ambient temperature and irradiance level. From the analysis and simulation results, this work establishes a proper model that can predict the output characteristics of PV cells under changes in weather conditions.

Production of an Anticoagulant Hirudin by Fed-batch and Continuous Cell Recycle Fermentations Using Recombinant Saccharomyces cerevisiae (유가식과 세포재순환 연속공정을 이용한 항혈전제 hirudin의 생산)

  • 최치민;김명동;이상기;서진호
    • KSBB Journal
    • /
    • v.13 no.4
    • /
    • pp.456-460
    • /
    • 1998
  • Fed-batch fermentations were carried out in order to improve the efficiency of hirudin production by recombinant Saccharomyces cerevisiae. A fed-batch fermentation done with the optimized semi-synthetic medium resulted in a maximum hirudin concentration of 342mg/$\ell$ by keeping a galactose concentrations between 10 and 30g/$\ell$ which corresponded to a 11.4-fold increase in hirudin concentration compared with the simple bach fermentation done with the same medium. Comparison of the chromatographic pattern of proteins in the growth medium clearly showed that the use of the semi-synthetic medium is more advantageous for separation of hirudin than the case o fusing the complex medium. Continuous cell recycle fermentation done at dilution rate of 0.1h-1 and an inlet galactose concentration of 100g/$\ell$ yielded a maximum hirudin productivity of 19.1mg hirudin/$\ell$$.$h.

  • PDF