• Title/Summary/Keyword: cell library

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Analysis of Seed Hair Formation Related Genes by EST Profiling in Carrot (Daucus carota var. sativa) (EST profiling을 통한 당근(Daucus carota var. sativa)의 종모 형성에 관련된 유전자 분석)

  • Hwang, Eun-Mi;Oh, Gyu-Dong;Shim, Eun-Jo;Jeon, Sang-Jin;Park, Young-Doo
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.1039-1050
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    • 2010
  • Carrot is one of the useful crops used abundantly in cooking in Western as well as Asia regions such as China and Korea. However, seed coats have hairs which should be removed to increase germination rate. Furthermore, because of seed hairs, farmers face several additional losses, such as time consumption, manpower, capital and so on, for seed handling. To prevent these problems, study of gene related hair formation using short-hair seed lines is required. We analyzed genes related to hair formation from seed through expressed sequenced tag (EST) profiling, based on the fact that the development of carrot seed hair is related to cellulose synthesis pathway in secondary cell wall synthesis stage. To study the gene expression related to hair formation of the carrot seed, a cDNA library was constructed by using the early maturation stage of the short-hair line (659-1) and hairy seed line (677-14). In short-hair (659-1) and hairy seed (677-14) lines, results from of EST profiling through BLASTX search analysis using the NCBI database showed that 172 and 224 unigenes had significant homology with known protein sequences, whereas 233 and 192 unigenes were not, respectively. All ESTs were grouped into 16 categories according to their putative functions. Twenty nine unigenes among all ESTs were considered to be genes regulating seed hair development from cellulose synthesis pathway during secondary cell wall synthesis stage; in results, 14 unigenes related to seed hair development were found only in hairy seed line.

Cloning of a Chitinase Gene of Xanthomonas sp. Isolated from Soil and its Expression in E. coli. (토양에서 분리된 Xanthomonas sp.의 Chitinase 유전자 cloning과 E.coli에서의 발현)

  • Kim, Ho-Sang;Seong, Ki-Young;Eun, Moo-Young;Hwang, Cher-Won
    • Applied Biological Chemistry
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    • v.41 no.2
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    • pp.125-129
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    • 1998
  • Xanthomonas sp. isolated from soil exhibited cell wall lytic activity of Candida albicans and secreted chitinase in chitin media. Especially, the chitinase activity was induced by chitin and reached a maximum level at 3 days culture in chitin media. We constructed genomic library of Xanthomonas sp. using cosmid vector in E. coli. Oligonucleotide probe was synthesized from the consensus sequence corresponding to chitinase active site, which was derived from the comparison of amino acid sequences of bacterial chitinase genes. Using this oligonucleotide probe, we screened the genomic library. By restriction enzyme mapping of the positive clones, we identified 4 independent clones which may contain the chitinase gene. One of the clones, named pXCH1 (1.2 kb insert), was further analyzed. Northern blot analysis indicated that is transcripts, 1 kb and 0.8 kb, were induced by chitin. When the cloned gene was induced by IPTG in E.coli cell, chitinase activity which was secreted onto culture media was not observed. However, when the cell was disrupted by using sonicator and then centrifuged, the supernatant exhibited chitinase activity. SDS-PAGE of the supernatant indicated that about 35 kDa protein was induced by IPTG. From these results, it was concluded that the cloned DNA was one of the chitinase genes of Xanthomonas sp.

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Cloning of the Genomic DNA Which Complements the Drug-Hypersensitivity of Saccharomyces cerevlsiae

  • Lee, Yun-Sik;Park, Kie-In
    • BMB Reports
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    • v.30 no.3
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    • pp.167-172
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    • 1997
  • The yeast Saccharomyces cerevisiae, mutant CH117, shows a drug-hypersensitivity (dhs) to cycloheximide, bleomycin, actinomycin D, 5-fluorouracil. nystatin, nigericin and several other antibiotics. CH 117 was also temperature-sensitive (ts). being unable to grow at $37^{\circ}C$ and secreted more invertase and acid phosphatase into the medium than the parent yeast. CH117 grows very slowly and the cell shape is somewhat larger and more sensitive to zymolyase than the wild type cells. Light microscopic and electron microscopic observation also revealed abnormality of the mutant cell wall. These characteristics indicate that CH117 has a defect in an essential component of the cell surface and that the cell wall which performs barrier functions has become leaky in the mutant. We screened a genomic library of wild type yeast for clones that can complement the mutation of CH117. A plasmid, pCHX1, with an insert of 3.6 kilobases (kbs) could complement the dhs and ts of CH117. Deletion and subcloning of the 3.6 kb insert showed that a gene for the complementation of mutant phenotypes was located in 1.9 kbs Puvll-Hindlll fragment.

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A Study for Design and Operational Features of Grid-Connected 30kW PVIB (30kW PVIB의 설계 및 구동특성에 관한 연구)

  • Park, Se-Joon;Yoon, Jeong-Phil;Choi, Hong-Jun;Shin, Yeong-Shik;Cha, In-Su;Kim, Dong-Mook;Lim, Jung-Yeol
    • 한국태양에너지학회:학술대회논문집
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    • 2008.04a
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    • pp.80-85
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    • 2008
  • A PVIB(Photovoltaic in Building) system is united by a constituent outer covering and can expect dual effects that reduce expenses for the establishment of a PV system. It is a profitable technology because it does not need a building as it is a stand alone PV system. This paper presents design, operational features analysis, and PCS(Power Conditioning System) of grid-connected 30kW PVIB set up on the library of Dongshin University. For a sustainable photovoltaic system in this area, the data of the PVIB system are collected and analyzed by monitoring system using LabView. PCS of the grid-connected PVIB system, also, is designed for optimal operation with characteristics suggested in this paper.

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2011, The Analysis Operating Characteristics of Photovoltaic System in Naju-city (2011년 나주시 태양광 발전 시스템의 운전특성)

  • Hyun, Jeong-Woo;Lee, Nam-Jin;Cha, In-Su;Kim, Dong-Mook;Choi, Jeong-Sik
    • 한국태양에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.359-363
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    • 2011
  • Building-integrated photovoltaics(BIPV)are increasingly incorporate into new domestic and industrial buildings as a proncipal or ancillary source of electrical power, and are on of the fastest growing segments of the photovoltaic industry. This paper presents operational features analysis and PCS(Power conversion System) factors of grid-connected 30kW BIPV on library of Dongshin University. The data consisted of insolation, Temperature, solar-cell performance and inverter performance are collected by IVIsion web monitoring system and analyzed. The analyzed data gave this paper effect elements of optimal operation.

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Alexandrium pacificum(Group IV) isolated from Jangmok Bay, Korea: Morphology, phylogeny, and effects of temperature, salinity, and nutrient levels on growth (장목만에서 분리한 유독 와편모조류 Alexandrium pacificum(Group IV): 형태, 계통 분류와 온도, 염분 및 영양염 농도 차이에 따른 성장 변화)

  • Kyong Ha Han;Joo Yeon Youn;Kyeong Yoon Kwak;Zhun Li;Wonchoel Lee;Hyeon Ho Shin
    • Korean Journal of Environmental Biology
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    • v.40 no.3
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    • pp.290-300
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    • 2022
  • A strain of Alexandrium species was established by isolating cells from Jangmok Bay, Korea. Its morphology and molecular phylogeny based on LSU rRNA gene sequences were examined. In addition, growth responses of this Alexandrium species to changes in temperature, salinity, and nutrient concentrations were investigated. This Alexandrium species from Jangmok Bay had a ventral pore on the 1', which was morphologically consistent with previously described Alexandrium tamarense and A. catenella. Phylogenetic analyses revealed that this isolate was assigned to A. pacificum (Group IV) within A. tamarense species complex. In growth experiments, relatively high growth rates and cell densities of A. pacificum (Group IV) were observed at 15℃ and 20℃. This species also grew under a wide range of salinity. This indicates that this Korean isolate of A. pacificum (Group IV) is a stenothermic and euryhaline species. In growth responses to changes in nutrient levels, enhanced growth rates and cell densities of A. pacificum(Group IV) were observed with additions of nitrate and phosphate. In particular, rapid uptakes of phosphate by A. pacificum (Group IV) were observed in experimental treatments, indicating that the increase in phosphate concentration could stimulate the growth of A. pacificum(Group IV).

VTR Servo Motor 제어용 회로의 IC화

  • 이광엽;임충빈;이문기;김용석;홍현석;김용환;김영웅
    • Proceedings of the Korean Institute of Communication Sciences Conference
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    • 1986.10a
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    • pp.91-94
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    • 1986
  • In this paper, a servo motor control IC for VTR is developed using standard cell library. All the cells are designed by single metal and the 3um design rule. A desinged circuit consists of circuits which generates a switching pulse and a control reference signal in VTR. The operation of VTR chip is verified by COSMOS simulator. Finally, layout is drawn by YOSELA.

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Design of a Deblocking Filter Circuit for MPEG-4 CODEC (MPEG-4 CODEC용 디블로킹 필터 회로 설계)

  • 김승호;조경순
    • Proceedings of the IEEK Conference
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    • 2003.07b
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    • pp.831-834
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    • 2003
  • 본 논문에서 기술하고 있는 디블로킹 필터는 ISO/1EC 14496-2 의 디블로킹 필터링 알고리즘[1][2]을 기반으로 한다. 한 개의 레지스터 뱅크를 이용한 효율적인 데이터 스케줄링을 통해 면적과 전력 측면에서 디블로킹 필터를 사용함으로써 생기는 오버헤드를 최소화 시켰으며, CIF 급 영상을 27MHz 동작주파수에서 실시간으로 처리할 수 있도록 설계 하였다. 0.25㎛ Standard Cell Library 로 합성한 결과 총 9800 게이트로 구성 되었으며, 외부 메모리의 도움 없이 동작 시키기 위해 4.4KByte의 버퍼가 사용되었다.

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Development of a Rectangle-based Layout Object Extraction Algorithm (직사각형을 기반으로 하는 레이아웃 개체추출 알고리즘)

  • 최용석;천익재;김보관
    • Proceedings of the IEEK Conference
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    • 2001.06b
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    • pp.113-116
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    • 2001
  • In this paper we present a new hierarchical layout object extraction algorithm, which is based on rectangles rather than edges. The original layout data is modeled as instances connected by wires. Each polygon shape is divided into a set of rectangles and the instances and wires are extracted and recognized from those rectangles together with their connection and size information. We have applied the algorithm to actual layouts. Experiments on several standard cell library demonstrate the effectiveness of the algorithm.

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