• The Transactions of the Korean Institute of Power Electronics
• /
• v.27 no.6
• /
• pp.526-534
• /
• 2022

The modular multilevel converter (MMC) has been widely applied to various industrial areas because of its various advantages and structural characteristics. Therefore, many methods for synthesizing the output voltage of MMC have been studied. Among these methods, phase-shifted pulse width modulation (PSPWM) is frequently used in MMC systems because it has diverse merits, such as excellent output qualities even with a small number of cells and uniform power distribution among cells. In this study, the total harmonic distortion (THD) of the output voltage is analyzed in accordance with the number of cells in one arm of a single-delta bridge cell MMC in order to compare PSPWM methods in terms of the THD of the output voltage. The physical characteristics of the triangle and sawtooth carrier waves used for the PSPWM and the mathematical modeling of output voltage are introduced. Then, the obtained results are verified through real-time simulation of a 1 MW single-delta bridge cell MMC system.

• Current Photovoltaic Research
• /
• v.11 no.1
• /
• pp.34-37
• /
• 2023

Solar energy had become the main energy industry of renewable energy along with hydroelectric power generation. One of the technologies that contributed to the popularization of photovoltaic power and the decrease in the unit price of photovoltaic modules was the large-area solar cell. However, as the area increased, the light receiving area increased and the current value increased accordingly. Since power loss occurs when the current value was large, the number of busbar was increased to increase the current collection rate, and a technology to lower the current value through half-cutting was developed. The bus bar of the solar cell served as a passage through which the generated current was transmitted. This was because when the number of busbar decreases, the moving distance of electrons increased, so the amount of power generation decreases and when it increases, shadows occured. An important aspect of the electrode design was the optimal balance of these busbars and number of fingers. Therefore, in this study, the characteristics of the solar cell according to the number of front bus bars of the large-area solar cell were simulated using Griddler 2,5 pro. After selecting the number of busbar with the best characteristics, the difference was compared by varying the number of fingers and a better direction for the number of cutting was presented.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.3
• /
• pp.333-337
• /
• 2000

An oil-degrading yeast, Yarrowia lipolytica 180, exhibits interesting cell surface characteristics under the growth on hydrocarbons. An electron microscopic study revealed that the cells grown on crude oil showed protrusions on the cell surface, and thicker periplasmic space and cell wall than the cell surface, and thicker periplasmic space and cell wall than the cells grown on glucose. Y. lipolytica cells lost its cell hydrophobicity after pronase(0.1 mg/ml) treatment. The strain produced two types of emulsifying materials during the growth on hydrocarbons; one was water-soluble extracellular materials and the other was cell wall-associated materials. Both emulsifying materials at lower concentration (0.12%) enhanced the oil-degrading activity of Moraxella sp. K12-7, which had medium emulsifying activity and negative cell hydrophobicity; however, it inhibited the oil-degrading activity of Pseudomunas sp. K12-5, which had medium emulsifying activity and cell hydrophobicity. These results suggest that the oil-degrading activity of Y. lipolytica 180 is closely associated with cell surface structure, and that a finely controlled application of Y.lipolytica 180 in combination with other oil-degrading microorganisms showed a possible enhancing efficiency of oil degradation.

• Transactions of the Korean hydrogen and new energy society
• /
• v.13 no.4
• /
• pp.313-320
• /
• 2002

The characteristics of the AISI bipolar plates have been investigated to replace the expensive graphite bipolar plates. It was found from the contact resistance evaluation of graphites, composites, and AISI that the contact resistance of AISI was the lowest, but it could approach to that of composites at higher compression forces. The single cell operation using the AISI bipolar plates revealed that the lower performance of the AISI single cell compared to the graphite one was due to not only the higher contact resistance but the flooding effect caused by high wettability of AISI. The performance of the AISI single cell could be improved if the channels were modified appropriately. The large size AISI single cell was operated to investigated the size effect on the performance.

• Transactions of the Korean hydrogen and new energy society
• /
• v.26 no.5
• /
• pp.493-498
• /
• 2015

The fuel cell vehicle is a type of hydrogen vehicle which uses a fuel cell to produce electricity, powering its on-board electric motor. The fuel cell vehicle driving principle is completely different from the internal combustion engine vehicle. In order to ensure the durable quality of the fuel cell vehicle, durability test mode considering the characteristics of the fuel cell must be developed. In this study, we derived the durability test mode profile through collecting and analyzing fuel cell vehicle driving data. Then, the accelerated durability test mode is developed by adding degradation conditions and is experimentally validated to have an acceleration factor of 5~6.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.42 no.2
• /
• pp.94-103
• /
• 2019

Self Organizing Map (SOM) is a neural network that is effective in classifying patterns that form the feature map by extracting characteristics of the input data. In this study, we propose an algorithm to determine the cell formation and the machine layout within the cell for the cell formation problem with operation sequence using the SOM. In the proposed algorithm, the output layer of the SOM is a one-dimensional structure, and the SOM is applied to the parts and the machine in two steps. The initial cell is formed when the formed clusters is grouped largely by the utilization of the machine within the cell. At this stage, machine cell are formed. The next step is to create a flow matrix of the all machine that calculates the frequency of consecutive forward movement for the machine. The machine layout order in each machine cell is determined based on this flow matrix so that the machine operation sequence is most reflected. The final step is to optimize the overall machine and parts to increase machine layout efficiency. As a result, the final cell is formed and the machine layout within the cell is determined. The proposed algorithm was tested on well-known cell formation problems with operation sequence shown in previous papers. The proposed algorithm has better performance than the other algorithms.

• IMMUNE NETWORK
• /
• v.20 no.1
• /
• pp.2.1-2.19
• /
• 2020

Acute viral infection or vaccination generates highly functional memory CD8 T cells following the Ag resolution. In contrast, persistent antigenic stimulation in chronic viral infection and cancer leads to a state of T-cell dysfunction termed T-cell exhaustion. We and other have recently identified a novel subset of exhausted CD8 T cells that act as stem cells for maintaining virus-specific CD8 T cells in a mouse model of chronic lymphocytic choriomeningitis virus infection. This stem cell-like CD8 T-cell subset has been also observed in both mouse and human tumor models. Most importantly, in both chronic viral infection and tumor models, the proliferative burst of Ag-specific CD8 T cells driven by PD-1-directed immunotherapy comes exclusively from this stem cell-like CD8 T-cell subset. Therefore, a better understanding of the mechanisms how CD8 T-cell subsets are regulated during chronic viral infection and cancer is required to improve the current immunotherapies that restore the function of exhausted CD8 T cells. In this review, we discuss the differentiation of virus-specific CD8 T cells during chronic viral infection, the characteristics and function of CD8 T-cell subsets, and the therapeutic intervention of PD-1-directed immunotherapy in cancer.

• Molecules and Cells
• /
• v.37 no.7
• /
• pp.562-567
• /
• 2014

Human Hertwig's epithelial root sheath/epithelial rests of Malassez (HERS/ERM) cells are epithelial remnants of teeth residing in the periodontium. Although the functional roles of HERS/ERM cells have yet to be elucidated, they are a unique epithelial cell population in adult teeth and are reported to have stem cell characteristics. Therefore, HERS/ERM cells might play a role as an epithelial component for the repair or regeneration of dental hard tissues; however, they are very rare population in periodontium and the primary isolation of them is considered to be difficult. To overcome these problems, we immortalized primary HERS/ERM cells isolated from human periodontium using SV40 large T antigen (SV40 LT) and performed a characterization of the immortalized cell line. Primary HERS/ERM cells could not be maintained for more than 6 passages; however, immortalized HERS/ERM cells were maintained for more than 20 passages. There were no differences in the morphological and immunophenotypic characteristics of HERS/ERM cells and immortalized HERS/ERM cells. The expression of epithelial stem cell and embryonic stem cell markers was maintained in immortalized HERS/ERM cells. Moreover, immortalized HERS/ERM cells could acquire mesenchymal phenotypes through the epithelial-mesenchymal transition via TGF-${\beta}1$. In conclusion, we established an immortalized human HERS/ERM cell line with SV40 LT and expect this cell line to contribute to the understanding of the functional roles of HERS/ERM cells and the tissue engineering of teeth.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.3
• /
• pp.761-766
• /
• 2012

Objective: Although various human cancer stem cells (CSCs) have been defined, their applications are restricted to immunocompromised models. Developing a novel CSC model which could be used in immunocompetent or transgenic mice is essential for further understanding of the biomolecular characteristics of tumor stem cells. Therefore, in this study, we analyzed murine lung cancer cells for the presence of CSCs. Methods: Side population (SP) cells were isolated by fluorescence activated cell sorting, followed by serum-free medium (SFM) culture, using Lewis lung carcinoma cell (LLC) line. The self-renewal, differentiated progeny, chemosensitivity, and tumorigenic properties in SP and non-SP cells were investigated through in vitro culture and in vivo serial transplantation. Differential expression profiles of stem cell markers were examined by RT-PCR. Results: The SP cell fraction comprised 1.1% of the total LLC population. SP cells were available to grow in SFM, and had significantly enhanced capacity for cell proliferation and colony formation. They were also more resistant to cisplatin in comparison to non-SP cells, and displayed increased tumorigenic ability. Moreover, SP cells showed higher mRNA expression of Oct-4, ABCG2, and CD44. Conclusion: We identified SP cells from a murine lung carcinoma, which possess well-known characteristics of CSCs. Our study established a useful model that should allow investigation of the biological features and pharmacosensitivity of lung CSCs, both in vitro and in syngeneic immunocompetent or transgenic/knockout mice.

• The Korean Journal of Health Service Management
• /
• v.10 no.3
• /
• pp.185-197
• /
• 2016

Objectives : The purpose of this study was to identify the influencing factors of cell phone addiction in adolescents and to construct a cell phone addiction model for adolescents. Methods : Data from the 5th Korean Child-Youth Panel Survey (KCYPS) was analyzed with the SPSS 20.0 and 23.0 AMOS programs. Results : According to the model of this study, depression had statistically significant direct effects while emotional characteristics and parental abuse had statistically significant indirect effects on cell phone addiction in adolescents. School life adjustment had statistically significant direct and indirect effects on cell phone addiction in adolescents. Conclusions : Based on the results of this study, it is necessary to develop prevention and intervention programs to combat cell phone addiction in Korean adolescents.