• Asian Pacific Journal of Cancer Prevention
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• v.15 no.22
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• pp.9967-9972
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• 2014

In order to prove whether downregulation of COX-2 (Cyclooxygenase-2) could modulate the epithelial-mesenchymal transition (EMT) of breast cancer, celecoxib and siRNA were respectively used to inhibit COX-2 function and expression in MDA-MB-231 cells. The EMT reversal effect in the RNAi treated group was better than that of the celecoxib group while there were no obvious differences in the medium $PGE_2$ levels between the two groups. The results show that COX-2 pathways may contribute considerably to EMT of breast cancer cells, partially dependent on the PGE2 cascade. Akt2, ZEB2 and Snail were measured to clarify the underlying mechanisms of COX-2 on EMT; COX-2 may modulate EMT of breast cancer by regulating these factors. This finding may be helpful to elucidate the mechanisms of selective COX-2 inhibitor action in EMT modulation in breast cancer.

• The KSFM Journal of Fluid Machinery
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• v.13 no.2
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• pp.41-47
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• 2010

The effect of incidence angle on the three-dimensional flow and aerodynamic loss in the tip leakage flow region downstream of a turbine rotor cascade has been investigated for two tip gap-to-chord ratios of h/c=0.0% (no tip gap) and 2.0%. The incidence angle is changed to be $i=-10^{\circ}$, $0^{\circ}$, and $5^{\circ}$. The results show that for $i=5^{\circ}$, secondary flows including the passage vortex are intensified noticeably, and there is a strong interaction between the passage and tip leakage vortices. For $i=-10^{\circ}$, however, the passage vortex is weakened significantly, so that there exists only a strong leakage-jet-like secondary flows near the casing wall. For h/c=0.0% and 2.0%, aerodynamic loss tends to increase with increasing i from $-10^{\circ}$ to $5^{\circ}$. A small increment of i in its positive incidence range results in a remarkable aerodynamic loss increase, while increasing i in the negative incidence range leads to a small change in the aerodynamic loss generation.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1417-1422
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• 2003

An electrical cascade impactor is a multistage impaction device to separate airborne particles into aerodynamic size classes using electrical method. We designed a real-time three-stage electrical low-pressure impactor, which is proper to nanometer sized environmental aerosols. Performance evaluation was carried out for stage 1 and 2. The monodisperse liquid dioctyl sebacate (DOS) particles were generated using condensation-evaporation followed by electrostatic classification using DMA (differential mobility analizer) for particles with diameters in the range of $0.04{\sim}0.8{\mu}m$. The evaluation of the electrical impactor is based on the use of two electrometers, one connected to the impaction plate of the impactor, and the other to the faraday cage as backup filter. The results showed that the experimental 50% cutoff diameters in the operation pressure were 0.53 and $0.12{\mu}m$ for stage 1 and stage 2. The effect of operation pressure on the cutoff diameter and the steepness of collection effcieicy curves is investigated.

• Journal of Korean Society for Atmospheric Environment
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• v.17 no.6
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• pp.441-450
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• 2001

A three stage impactor with the cutoff diameters of 1, 2.5, and 10$\mu\textrm{m}$ in aerodynamic diameter was developed and tested. The gravimetric method and the particle counting method were utilized to evaluate the collection performance of each stage. A vibrating orifice aerosol generator was employed to generate monodisperse test aerosols larger that 2$\mu\textrm{m}$ in diameter. Polystyrene latex (PSL) particles smaller than 2$\mu\textrm{m}$ in diameter were generated by an atomizer and the particle number concentration was measured by an Aerodynamic Particle Sizer Spectrometer. The experimental cutoff diameters obtained from the particle collection efficiency curves are in good agreement with the designed values. The square roots of Stokes number at 50% collection efficiency for stage 1, 2, and 3 are 0.42, 0.48, and 0.45, respectively. Effects of the particle bounce and the impaction plate on the collection efficiency were investigated. The collection efficiency curves including effect of the particle bounce were also compared with those of the MOUDI cascade impactor.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.16 no.10 s.115
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• pp.1082-1089
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• 2006

In this study, advanced computational analysis system has been developed in order to investigate flow-induced vibration(FIV) phenomenon for general stator-rotor cascade configurations. Relative movement of the rotor with respect to stator is reflected by modeling Independent two computational domains. Fluid domains are modeled using the unstructured grid system with dynamic moving and local deforming methods. Unsteady, Reynolds-averaged Wavier-stokes equations with one equation Spalart-Allmaras and two-equation SST ${\kappa}-{\varepsilon}$ turbulence models are solved for unsteady flow problems and also relative moving and vibration effects of the rotor cascade are fully considered. A coupled implicit time marching scheme based on the Newmark integration method is used for computing the governing equations of fluid-structure interaction problems. Detailed vibration responses for different flow conditions are presented and then vibration characteristics are physically investigated in the time domain as computational virtual tests.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.03a
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• pp.6-15
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• 2008

This paper deals with the aeroelastic instability of vibrating multiple blade rows under aerodynamic coupling with each other. A model composed of three blade rows, e.g., rotor-stator-rotor, where blades of the two rotor cascades are simultaneously vibrating, is considered. The displacement of a blade vibrating under aerodynamic force is expanded in a modal series with the natural mode shape functions, and the modal amplitudes are treated as the generalized coordinates. The generalized mass matrix and the generalized stiffness matrix are formulated on the basis of the finite element concept. The generalized aerodynamic force on a vibrating blade consists of the component induced by the motion of the blade itself and those induced not only by vibrations of other blades of the same cascade but also vibrations of blades in another cascade. To evaluate the aerodynamic forces, the unsteady lifting surface theory for the model of three blade rows is applied. The so-called k method is applied to determine the critical flutter conditions. A numerical study has been conducted. The flutter boundaries are compared with those for a single blade row. It is shown that the effect of the aerodynamic blade row coupling substantially modifies the critical flutter conditions.

• Journal of Ginseng Research
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• v.46 no.1
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• pp.54-61
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• 2022

Ginseng has been widely studied due to its various therapeutic properties on various diseases such as cardiovascular disease (CVD). Cardiovascular disease has been canonically known to be caused by high levels of low-density lipoproteins (LDL) in the bloodstream, in addition to the impaired vasodilatory effects of cholesterol. However, current research on CVD has revealed a cascade of mechanisms involving a series of events that contribute to the progression of CVD. Although this has been elucidated and summarized in previous studies the detailed correlation between platelet aggregation and innate immunity that plays an important role in CVD progression has not been thoroughly summarized. Furthermore, immune cell subtypes also contribute to the progression of plaque formation in the subendothelial layer. Thrombus formation and the coagulation cascade also have a vital role in the progression of atherosclerosis. Hence, in this mini review we aim to elucidate, summarize, and propose the potent therapeutic effect of ginseng on CVD, mainly on platelet aggregation, plaque formation, and thrombus formation.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.278.1-278.1
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• 2002

In the previous study, we reported that NQ12, a vitamin K antagonist. showed a potent antithrombotic and antiplatelet activities. In order to elucidate the antiplatelet activity of NQ12. we investigated the effect of NQ12 on arachidonic acid cascade parameters such as cPLA2. cyclooxygenase (COX), and the downstream production such as TxA2, PGD2 and 12-HETE. N012 inhibited COX activity in a concentration-dependent manner in U937 cells. (omitted)

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.5
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• pp.335-345
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• 2022

Pyroptosis is an inflammatory form of programmed cell death that is linked with invading intracellular pathogens. Cardiac pyroptosis has a significant role in coronary microembolization (CME), thus causing myocardial injury. Tanshinone IIA (Tan IIA) has powerful cardioprotective effects. Hence, this study aimed to identify the effect of Tan IIA on CME and its underlying mechanism. Forty Sprague-Dawley (SD) rats were randomly grouped into sham, CME, CME + low-dose Tan IIA, and CME + high-dose Tan IIA groups. Except for the sham group, polyethylene microspheres (42 ㎛) were injected to establish the CME model. The Tan-L and Tan-H groups received intraperitoneal Tan IIA for 7 days before CME. After CME, cardiac function, myocardial histopathology, and serum myocardial injury markers were assessed. The expression of pyroptosis-associated molecules and TLR4/MyD88/NF-κB/NLRP3 cascade was evaluated by qRT-PCR, Western blotting, ELISA, and IHC. Relative to the sham group, CME group's cardiac functions were significantly reduced, with a high level of serum myocardial injury markers, and microinfarct area. Also, the levels of caspase-1 p20, GSDMD-N, IL-18, IL-1β, TLR4, MyD88, p-NF-κB p65, NLRP3, and ASC expression were increased. Relative to the CME group, the Tan-H and Tan-L groups had considerably improved cardiac functions, with a considerably low level of serum myocardial injury markers and microinfarct area. Tan IIA can reduce the levels of pyroptosis-associated mRNA and protein, which may be caused by inhibiting TLR4/MyD88/NF-κB/NLRP3 cascade. In conclusion, Tanshinone IIA can suppress cardiomyocyte pyroptosis probably through modulating the TLR4/MyD88/NF-κB/NLRP3 cascade, lowering cardiac dysfunction, and myocardial damage.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.2
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• pp.197-205
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• 2003

A cascade impactor is a multistage impaction device used to separate airborne particles into aerodynamic size classes. A micro-orifice impactor uses micro-orifice nozzles to extend the cut sizes of the lower stages to as small as 0.05 ${\mu}{\textrm}{m}$ in diameter without resorting to low pressures or creating excessive pressure drops across the impactor stages. In this work, the phenomenon of particle clogging in micro-orifice nozzles was experimentally investigated for a commercial micro-orifice uniform deposit impactor (MOUDI). It was observed, using an optical microscope, that the micro-orifice nozzles of the final stages were partially clogged due to particle deposition during the aerosol sampling. Therefore the pressure drops across the nozzles were higher than the nominal values given by the manufacturer. To examine the effect of particle clogging in micro-orifice nozzles, the particle collection efficiency of the MOUDI was evaluated using an electrical method for fine particles with diameters in the range of 0.1-0.6 ${\mu}{\textrm}{m}$. The monodisperse liquid dioctyl sebacate (DOS) particles were used as test aerosols. A faraday cage was employed to measure the low-level current of the charged particles upstream and downstream of each stage. It was found that the collection efficiency curves shifted to correspond to smaller orifice sizes, and the 50-% cutoff sizes were much smaller than those given by the manufacturer for the three stages with nozzles less than 400 ${\mu}{\textrm}{m}$ in diameter.