• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 2008.03a
• /
• pp.143-149
• /
• 2008

A numerical study was performed for a vane of a compressor with a high-turning angle and meridional divergence. At first, the effect of the suction position was discussed. Then, the optimal suction position was applied to the cascades with the aspect ratio of 2.53 and 0.3, respectively, to get the knowledge of the effect of the endwall boundary layer removal on the secondary flow along the blade height. At last, using the critical principles of the three-dimensional separation, the topological structures of the flow patterns of the body surfaces and the separation configurations were discussed in detail. The results show that the largest reduction of the total loss can be achieved when the suction slot is near the suction side. The topological structure as well as the separation configuration varies due to boundary layer removal, which restrains the flow separation at the corner and delays or depresses the separation on the suction surface. Compared with the original cascade, the cascade with the endwall boundary layer removal has a higher blade loading along the most span. Furthermore the flow loss decreases and distributes uniformly along the span.

• Journal of Life Science
• /
• v.13 no.1
• /
• pp.118-127
• /
• 2003

$\rho$-Fluorophenylalanine (FPA), a phenylalanine analog, is able to induce apoptotic cell death of human acute leukemia Jurkat T cells. To better understand the mechanism by which FPA induces apoptotic cell death, the effect of ectopic expression of antiapoptotic proteins, Bcl-2 and Bcl-xL, on FPA-induced apoptosis was investigated by employing lurkat T cells transfected with Bcl-2 gene (JT/Bcl-2) or Bcl-xL gene (1/Bcl-xL) and Jurkat T cells transfected with vector (JT/Neo or J/Neo). When Jurkat T cells, JT/Neo or J/Neo, were exposed to FPA at concentrations ranging from 0.63 to 5.0 mM, the cell viability determined by MTT assay declined in a dose-dependent manner. In addition, apoptotic DNA fragmentation along with several apoptotic events such as caspase-8 activation, Bid cleavage, mitochondrial cytochrome c release, caspase-9 activation, caspase-3 activation, and degradation of PARP was induced. However, the FPA-induced cytotoxic effect, activation of caspase-8, and cleavage of Bid were significantly abrogated by ectopic expression of Bcl-2 or Bcl-xL. At the same time, there was marked reduction in the level of cytochrome c release from mitorhondria, caspase-9 activation, caspase-3 activation, and degradation of PARP. These results indicate that caspase-8 activation, Bid cleavage, and mitochondrial cytochrome c release with subsequent activation of the caspase cascade are negatively regulated by Bcl-2 or Bcl-xL, and are thus required for FPA-induced apoptosis in Jurkat T cells

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.13 no.4
• /
• pp.277-282
• /
• 2020

This study was initiated to find a way to resolve electronic equipment as it could be affected by multiple environments. The effect of setting the exponential constant wave (iExp) in the telescopic cascade comparator to the SET (Single Event Transient) environment was tested. In this paper, the radio wave delay was measured at 0.46 ㎲ and the gain at 0.713 in the telescopic cascade comparator without setting the SET situation. FET T0 (M6) was measured to have a large spike at 11㎲ to 15㎲ in the telescopic cascade comparator entering the SET situation. FET T1 (M5) has shorted output signals from 10 ㎲ to 16 ㎲. FET T2 (M3) represented a shorted output signal, and FET T3 (M4) measured the output waveform in the form of a large spike waveform. The FET T4 (M1) and FET T5 (M2) are spiky signals. And at all FETs, the propagation delay was changed from 0.45㎲ to 0.54㎲. In summary, The FET element in the telescopic cascade comparator in SET situation was measured to be greatly affected.

• The KSFM Journal of Fluid Machinery
• /
• v.8 no.4 s.31
• /
• pp.27-38
• /
• 2005

The present study investigated the effect of relative position of the blade on blade surface heat transfer. The experiments were conducted in a low speed wind tunnel with a stationary annular turbine cascade. The test section has a single turbine stage composed of sixteen guide vanes and blades. The chord length of the blade is 150 mm and the mean tip clearance of the blade is $2.5\%$ of the blade chord. The Reynolds number based on blade inlet velocity and chord length is $1.5{\times}105$ and mean turbulence intensity is about $3\%$. To investigate the effect of relative position of blade, the blade at six different positions in a pitch was examined. For the detailed mass transfer measurements, a naphthalene sublimation technique was used. In general, complex heat transfer characteristics are observed on the blade surface due to various flow characteristics, such as a laminar flow separation, relaminarization, flow acceleration, transition to turbulence and tip leakage vortices. The results show that the blade relative position affects those heat transfer characteristics because the distributions of incoming flow velocity and turbulence intensity are changed. Especially, the heat transfer pattern on the near-tip region is significantly affected by the relative position of the blade because the effect of tip leakage vortex is strongly dependent on the blade position. On the pressure side, the effect of blade position is not so significant as on the suction side surface although the position and the size of the separation bubble are changed.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.15 no.1
• /
• pp.229-240
• /
• 1991

The large camber angle theory of turbomachine blade of compressor has been developed recently for the two-dimensional flow by Hawthorn, et al. However, in the above theory it was assumed that the fluid was incompressible and inviscid, and the blades had no thickness. In this study, the flow in a blade cascade being mounted in parallel fashion with blade of arbitrary thickness is studied in order to determine the effects of the camber angle on the performance characteristic of the blade section under the consideration of compressibility and viscosity of fluid. The panel method is used for potential flow analysis. The flow in the boundary-layer is obtained by solving the integral boundary-layer structure through the laminar, transitional , and turbulent flow using the pressure field determined from the potential flow. And then the viscous-inviscid interaction scheme is used for interaction of these two flows. For the determination of the variation in the outlet fluid angle influenced by deviation in cascade flow, the superposition method which is used for single foil is introduced in this analysis. By the introduction of this method, the effects of the deviation on outlet fluid angle and the resulting fluid angle are made to adjust for oneself through the calculation. As the result of this study, the blade of large camber angle, large incidence angle, large pitch-chord ratio has large viscous and compressible effect than those of small camber angle. Lift force increase as camber angle increases, but above 60.deg. of camber angle, lift force decrease as camber angle increases. But drag force increases linearly with camber angle increases in the entire region.

• Journal of Advanced Marine Engineering and Technology
• /
• v.34 no.1
• /
• pp.46-52
• /
• 2010

In this study, several types of natural gas liquefaction processes using two staged Inter-cooler are simulated and designed to secure a competitiveness in the industry of natural gas liquefaction plant. These processes are based on basic cascade process, and all of these are improved with two staged compressors type. One of types is applied Inter-cooler to each cycle such as propane, ethylene, methane, the other type is applied Inter-cooler to whole cycle. These processes are compared characteristics of performance with basic process. Cascade process with two staged Inter-cooler in the whole cycle is on the top ranked with increment ratio of COP about 13.7 ~ 20.5%, and yield efficiency of this process are improved comparing with the basic process by 23.8% ~ 35% lower specific power, respectively.

• Journal of Mechanical Science and Technology
• /
• v.18 no.8
• /
• pp.1435-1450
• /
• 2004

Experimental data are presented which describe the effects of a combustor-level high free-stream turbulence on the near-wall flow structure and heat/mass transfer on the endwall of a linear high-turning turbine rotor cascade. The end wall flow structure is visualized by employing the partial- and total-coverage oil-film technique, and heat/mass transfer rate is measured by the naphthalene sublimation method. A turbulence generator is designed to provide a highly-turbulent flow which has free-stream turbulence intensity and integral length scale of 14.7% and 80mm, respectively, at the cascade entrance. The surface flow visualizations show that the high free-stream turbulence has little effect on the attachment line, but alters the separation line noticeably. Under high free-stream turbulence, the incoming near-wall flow upstream of the adjacent separation lines collides more obliquely with the suction surface. A weaker lift-up force arising from this more oblique collision results in the narrower suction-side corner vortex area in the high turbulence case. The high free-stream turbulence enhances the heat/mass transfer in the central area of the turbine passage, but only a slight augmentation is found in the end wall regions adjacent to the leading and trailing edges. Therefore, the high free-stream turbulence makes the end wall heat load more uniform. It is also observed that the heat/mass transfers along the locus of the pressure-side leg of the leading-edge horseshoe vortex and along the suction-side corner are influenced most strongly by the high free-stream turbulence. In this study, the end wall surface is classified into seven different regions based on the local heat/mass transfer distribution, and the effects of the high free-stream turbulence on the local heat/mass transfer in each region are discussed in detail.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.35 no.2
• /
• pp.143-148
• /
• 2007

An impulse turbine, which is a main component of a liquid rocket engine, needs to be a small size with light weight and generate large power. Since the impulse turbine is being operated under complicated supersonic conditions, flow analysis and performance prediction largely depend on CFD technique. In order to increase the reliability of the prediction code, however, it often requires an experimental data to compare. In this research a rotating turbine rotor with multiple blades is simulated with a two-dimensional stationary cascade to check the effect of major flow parameters. Mach number is measured at nozzle exit by using a pitot tube and the blade thrust was also measured with a load cell. The measured thrust coefficient and the power are compared well with the designed conditions, which proves the design procedures are properly taken.

• The Korea Journal of Herbology
• /
• v.21 no.2
• /
• pp.37-46
• /
• 2006

Objectives : The purpose of this study was to investigate the effect of Dangguibohyultang (DB) and its combination (DB-I; Astragali membraneus BUNGE : Angelica gigas NAKAI=5:1, DB-II; Astragali membraneus BUNGE:Angelica gigas NAKAI=1:1, DB-III; Astragali membraneus BUNGE:Angelica gigas NAKAI=1:5,) on apoptosis in human colorectal adenocarcinoma HCT116 cells. Methods : To study the cytotoxic effect of methanol extract of DB-I, DB-II and DB-III on HCT116 cells, the cell viability was determined by XTT reduction method and ttypan blue exclusion assay. To confirm the induction of apoptosis, the cleavage of poly ADP-ribose polymerase (PARP), a substrate for caspase-3 and a typical sign of apoptosis, and the activation of procaspase-3, -8 and -9 were examined by western blot analysis. Furthermore, DB-induced apoptosis was confirmed by DNA fragmentation. The release of cytochrome C from mitochondria to cytosol, the level of Bcl-2 and Bax, and the expressions of Raf/MEK/ERK were examined by western blot analysis. Results : DB-I and DB-II reduced proliferation of HCT116 cells in a dose-dependent manner. DB-I and DB-II decreased procaspase-3, -8, -9 levels in a dose-dependent manner and induced the clevage of PARP. DB-I and DB-II also triggered the mitochondrial apoptotic signaling by increasing the release of cytochrome C from mitochondria to cytosol, decreasing of anti-apoptotic Bcl-2, and increasing of pro-apoptotic Bax. DB-I and DB-II decreased the activation of Ras/Raf/MEK/ERK cascade in a dose-dependent manner. Conclusion : These results suggest that DB-I and DB-II induce apoptosis via mitochondrial pathway in HCT116 cells. Furthermore, Raf/MEK/ERK cascade is involved in DB-induced apoptosis. These results suggest that DB is potentially useful as a chemotherapeutic agent in human liver cancer.

• Development and Reproduction
• /
• v.6 no.1
• /
• pp.1-6
• /
• 2002

To elucidate the mechanism underlying the embryotropic effect of extracellular matrix(ECM) on the preimplantation development of mammalian embryos, the involvement of mitogen-activated protein kinase(MAPK) downstream the integrin signaling was examined in mouse blastocysts. Blastocysts were cultured in the presence of growth factor-reduced(GFR) Matrigel(0.5％, v/v). MAPK activity was measured by in vitro phosphorylation of myelin basic protein by the Erk1/2 antibody immunoprecipitates of embryonic extract following the Matrigei treatment. MAPK activity of the early blastocysts rapidly increased within 10 min fo1lowing the Matrigel treatment. When the embryos were cultured for 12 h in the presence of Matrigel, the MAPK activity was significantly higher than that ot the control embryos. PD098059, a MAPK kinase(MEK) inhibitor, attenuated the effect of Matrigel on the change in MAPK activity. Taken together, it suggested that the embryotropic effect of ECM proteins might be mediated by the activation of MAPK cascade.