• The Plant Pathology Journal
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• 제17권2호
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• pp.101-105
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• 2001

A disease survey on the carnation (Dianthus caryophyllus L.) wilt was conducted during the high temperature period (June through August) and the low temperature period (February through May) in 58 greenhouses of its major cultivation areas, including Pusan, Kimhae, and Changwon in Korea from 1998 to 1999. The disease incidence was averaged 5.4% and 11.9% in the low and high temperature periods, respectively. Severe damage was found in summer with high incidences of around 50% in some greenhouses. Close examination of the symptoms and isolation of the causal agent revealed that there was a new disease different from Fusarium wilt caused by Fusarium oxysporum f. sp. dianthi, which was determined as the stub dieback caused by F. was cetermined as the stub dieback caused by F. graminearum (teleomorph : Gibberella zeae). The stub dieback symptoms involved brown rot of stem that started usually from the portion of cutting without discoloration of inner vascular tissues. Seven out of 38 isolates from the wilted plants were identified as F. graminearum, while the others as F. oxysporum f. sp. dianthi. Mycological characteristics of the stub dieback pathogen including colony color, absence of microconidia, and the shape of macroconidia, were consistent with F. graminearum previously described. This is the first report of the carnation stub dieback in Korea.

• 한국식물병리학회지
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• 제10권4호
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• pp.314-318
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• 1994

Incidence of stem rot on carnation (Dianthus caryophyllus L.) ranged 11 to 29% in Namwon and Chongup area during the growing seasons from 1993 to 1994. Among 129 isolates from carnations in Namwon, 77 isolates were identified as Rhizoctonia solani, 38 isolates were Fusarium oxysporum and 14 isolates were not identified. Among 169 isolates in Chongup, 19 isolates were identified as R. solani, 106 isolates as F. oxysporum and 44 isolates were not identified. Among 77 isolates of R. solani isolated from the specimens of Namwon, 52 isolates were classified as anastomosis group AG 2-2 by anastomosis test, 14 isolates as AG 2-1 and 11 isolates as AG 4. Among 19 isolates from specimens of Chongup, 14 isolates were classified as anastomosis group AG 2-2 and 5 isolates as AG 4. Pathogenicity tests revealed that isolates of F. oxysporum and R.solani AG 2-2 were highly virulent and isolates of R.solani AG 2-1 and AG 4 were mildly virulent.

• 생물환경조절학회지
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• 제7권2호
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• pp.116-122
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• 1998

본 연구는 양액재배 카네이션의 생육을 증진시키기 위하여 최적 재배작형과 배지 종류를 구명코자 실시되었다. coir, perlite, coir＋perlite(1：1. v/v)등 3 종류의 배지와 5월 1일 정식, 9월 1일 정식 등 2 종류의 작 형에서 재밴한 후 생육을 조사 비교하였다. 절화 수확기에 초장과 줄기의 직경은 5월 1일에 정식한 식물 보다 9월 1일에 정식한 식물에서 컸다. 5월 1일에 정식한 경우는 식물체 줄기의 직경과 초장생장이 극히 저조하여 매우 약해졌다 꽃의 폭과 무게도 9월 1일에 정식한 식물체가 5월 1일에 정식한 식물체보다 증가되었다 꽃잎수는 정식시기에 따라 뚜렷한 경향을 보이지 않았다 개화소요일수는 5월 1일에 정식한 경우가 9월 1일에 정식한 경우보다 50일 이상 단축되는 결과를 보였다 재배배지별로는 처리간 큰 차이는 보이지 않았지만 coir를 사용했을 때 초장과 꽃잎수가 증가되었다. 실험결과 카네이션의 양액재배에서는 고온기를 회피한 작형, 예컨데 9월 상순, 중순에 정식할 필요가 있고 재배배지로 coir의 사용이 긍정적인 것으로 확인되었다

• 한국육종학회지
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• 제42권6호
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• pp.645-648
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• 2010

분홍색 스프레이 카네이션 품종 'Lyra'는 노랑색 스프레이 품종 'Ballantyne'와 적색 스프레이 품종 'Chateau'을 인공 교배하여 국립원예특작과학원에서 육성되었다. 'Lyra'는 1999년에 교배하여 2001-2003년까지 품종 특성을 조사하였고, 2003년에 최종 선발하였다. 'Lyra'는 밝은 분홍색 절화 스프레이 품종으로 다 분지성이고 절화장이 길며 향기가 있는 조기 개화성 품종이다. 절화 수명은 7일이고 시들음병에는 중간 정도의 저항성이 있다. 겨울철 야간에는 $8^{\circ}C$ 이상으로 관리하여 하고 햇볕이 약하면 줄기가 연약해 지므로 최대한 햇볕을 많이 받게 해주며 여름철에는 기온이 $30^{\circ}C$ 이상 올라가지 않도록 관리해 주어야 한다.

• 한국식물병리학회지
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• 제14권2호
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• pp.115-119
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• 1998

Root rot of carnation caused by isolates of Phytophthora was found at Kongju, 1996. Infected plants appeared symptoms of wilting, brown discoloration on collar area and consequently led to death. The causal pathogen was identified as Phytophthora nicotianae based on morphological, cultural and physiological characteristics. Mycelium was grown to rosette colony pattern on corn meal agar and the growth was 10.2 mm/day on 10% clarified V8 medium at $25^{\circ}C$. Swelling with radiating hyphae was formed in water and on agar within 7 days. Chlamydospores were abundantly produced on agar within 7 days. Sporangium was prominent papillate, 2~3 apex, 1.2 : 1 l/b ratio, lateral attacment on sporangiospore and was rarely produced on solid media but produced in water. The shape of sporangium was spherical or ovoid, and the size was 34~73$\times$32~60 (av. 33$\times$66.5) ${\mu}{\textrm}{m}$ in dimension. The isolates were heterothallic, and mating type A2. Oogonium was spherical, ovoid, 26~36 (av. 36 ${\mu}{\textrm}{m}$) in diameter, and antheridium was amphygynious to oogonia. The fungus was able to grow 10~35$^{\circ}C$, and optimal temperature was 27$^{\circ}C$.

• Journal of Plant Biotechnology
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• 제48권2호
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• pp.93-99
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• 2021

This study was conducted to develop an Agrobacterium-mediated genetic transformation protocol for the carnation cv. "Jinju" to counteract its ethylene sensitivity. The new protocol involves the use of an improved shoot regeneration medium, optimized minimal concentrations of the selective agent, a pre-culture period, and co-cultivation periods. Silver nanoparticles (NAg) added at a concentration of 2.0 μM to the Murashige and Skoog (MS) basal shoot regeneration medium supplemented with 0.1 mg/L indole-3-butyric-acid (IBA) and 0.2 mg/L thidiazuron (TDZ) improved the shoot regeneration efficiency, number of shoots per explant, and plant growth compared to the control without the addition of NAg. The phosphinothricin (PPT) concentration of 1.0 mg/L was determined to be the minimal and optimal concentration for the selection of putative transgenic plants. When the explants were infected with Agrobacterium cells harboring the acdS gene, the explants that were pre-cultured for three days induced more putative transgenic plants than those that were co-cultivated for four days. Therefore, we expect that the results of this study will benefit researchers who are developing genetic transformations of carnations.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.47-47
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• 2020

In this study, we aimed to study carnation italian ringspot virus (CIRV) in Erigeron annuus (L.) Pers. in Bonghwa County, Korea. The collected samples showed mosaic and malformation symptoms. To identify the virus species, we performed high-throughput sequencing, reverse transcription polymerase chain reaction, and cloning. The virus was confirmed to be an unreported species, and therefore we performed genome sequencing of the samples. The complete genome was 4,746 nucleotides in length. The CIRV contained five open reading frames (ORFs), and it showed the typical features of members of the genus Tombusvirus. Phylogenetic analyses revealed that ClRV isolates had the highest nucleotide identities with the CZ isolate (95.89%) from Korea. In recent years, these viruses have sporadically been reported in floral scent and medicinal plants. This research found the first natural host infected with CIRV, and provides baseline information to determine the correlation between weeds and crops.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.150.2-150
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• 2003

Carnation mottle carmovirus(CarMV) was isolated from Lilium spp. in Korea. This isolate, CarMV, was done bioassay, which plants were Dianthus caryophyllus, Gomphrena globosa, Chenopodium amaranticolor, Dianths chinensis. CarMV was propagated on the leaves of Chenopodium amaranticolor with the crude-sap inoculation method and purified by Mossops method(1976). We produced antiserum against CarMV and analyzed the antiserum specificity with ELISA, Gel diffusion method, and Rapid Immunofilter Paper Assay (RIPA). From these results of the assay, RIPA method was simple and rapid for CarMV detection. We have established successfully the CarMV detection system. CarMV coat protein gene was amplified by RT-PCR with specific primers and sequencing analysis was done.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.319.1-319
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• 1998

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 제19회 생물과학 심포지움 강연록
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• pp.15-24
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• 1998

No Abstract, See Full Text