• IMMUNE NETWORK
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• 제1권2호
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• pp.162-169
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• 2001

Background: Nitric oxide (NO), a cytotoxic molecule is produced in various tissues including tumor cells during interleukin-2 (IL-2) therapy . Lymphokine-activated killer (LAK) cells are induced during IL-2 therapy, and have cytotoxic activity against tumor cells. The current study investigated the effects of NO synthesized in target cells or exposure of target cells to NO on the sensitivity of target cells to LAK cell cytotoxicity. Methods: Cytotoxicity was measured using 4 h chromium release assays. LAK cells which were induced by a 4 day incubation of BALB/c mouse splenocytes with IL-2 (6,000 IU/mL) were employed as effector cells. RD-995 skin tumor cells originated from a C3H/HeN mouse were employed as target cells. NO synthesis in target cells was induced by a 24 h incubation of RD-995 cells with $IFN{\gamma}$ (25 U/mL), TNF (50 U/mL) and IL-1 (20 U/mL). S-nitrosyl acetylpenicillamine (SNAP), an NO donor, was used to expose target cells to NO. $N^G$-monomethyl-L-arginine (MLA) and carboxy-PTIO were added during cytotoxicity assays to inhibit NO synthesis, and to scavenge NO produced by target cells, respectively. Results: Sensitivity of NO-producing RD-995 cells to LAK cell cytotoxicity was decreased by addition of MLA and carboxy-PTIO during cytotoxicity assays. However, the two reagents had no effect on the sensitivity of non-NO-producing RD-995 cells. Pretreatment of RD-995 target cells with SNAP increased the sensitivity in comparison with untreated cells. Conclusions: Sensitivity of target cells to LAK cell cytotoxicity is increased by target cell NO synthesis or exposure to NO. Further studies are needed to evaluate whether these in vitro results have relevance to in vivo phenomena.

• 디지털콘텐츠학회 논문지
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• 제16권3호
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• pp.483-492
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• 2015

최근에 비만을 치료하기 위한 요법 중에 카복시테라피 치료법이 있다. 이 방법은 지방이 과도하게 축적된 부위에 이산화탄소($CO_2$)를 주입하여 치료하는 방법으로 최근 각광받고 있다. 가스통에 담겨 있는 이산화탄소를 피부에 주입하려면 주입기가 필요하며, 건강을 위해 주입량을 정확히 조절할 수 있어야 한다. 본 논문에서는 국내에서 제작한 CARBO 3000이라는 이산화탄소 주입기의 유량주입 방법을 분석하고 단점을 파악한다. 유량 센서와 일정 유량이 출력되게 고정된 유속 조절기를 추가하여 주입된 정확한 유량을 보여줄 수 있는 모듈을 구현한다. 구현을 위해 하드웨어적으로는 CPU와 LCD, 유속 조절기를 선정하였으며 솔레노이드 밸브와 유량센서를 선정하고 구동을 시킬 수 있는 회로를 설계한다. 소프트웨어적으로는 주입된 이산화탄소의 양을 판별할 수 있는 GUI를 설계하고 유량을 제어할 수 있는 유량 제어 알고리즘을 설계한다.