• Title/Summary/Keyword: carbon to nitrogen ratio

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Effect of Carbon and Nitrogen Source on Somatic Embryogenesis in Suspension Culture of Ligusticum chuanxiang Hort. (천궁의 현탁배양에서 탄소원과 질소원이 체세포배 형성에 미치는 영향)

  • Chae, Young-Am;Park, Sang-Un
    • Korean Journal of Medicinal Crop Science
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    • v.2 no.1
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    • pp.44-50
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    • 1994
  • This study was carried out to select the appropriate medium(especially, carbon and nitrogen source ) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of chuanxiang Hort. Suitable medium for somatic embryo formation was MS medium. The half strength MS medium was effective for somatic embryo development. Sucrose was the most effective carbon source for somatic embryo formation, however, production of somatic embryos was reduced at higher concentration of sucrose. Effects of suger was the same as sucrose. Somatic embryo formation was higher as the decrease of $NH_{4}NO_3$, and optimum ratio of $KNO_3\;:\;NH_4NO_3$ was 825 : 238mg /1. Regenerated plant was obtained in MS basal medium and survival late of plantlet was 60-70% after transplanted directly to the vermiculite.

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Reduction of Lean VOC Emission by Reforming with a Rotating Arc Plasma and Combustion with a Turbulent Partially-Premixed Flame (난류 부분예혼합화염과 로테이팅 아크 플라즈마를 이용한 난연성 유증기의 연소처리)

  • Ahn, Taekook;Lee, Daehoon;Park, Sunho
    • Journal of the Korean Society of Combustion
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    • v.22 no.1
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    • pp.23-31
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    • 2017
  • Large-scale fuel tanks emit massive amount of hardly-combustible VOC mixtures which are light hydrocarbon species in dilution with nitrogen and carbon dioxide. We have developed a lab-scale burner to combust those VOC mixtures by use of a turbulent partially-premixed flame as a pilot flame. For a higher HC treatment ratio, the mixture gases were reformed by a rotating arc plasma device. The results showed that the nitrogen mole fraction and the injecting speed of the VOC mixture influence on the performance of the burner. It was also found that the size of the pilot flame and the power supplied to the plasma device determine the overall HC treatment ratio and the concentrations of CO and NOx in the exhaust gas.

Effects of Microalloying Elements on Microstructures and Toughness of Simulated HAZ in Quenched and Tempered Steels

  • Chang, W.S.;Yoon, B.H.
    • International Journal of Korean Welding Society
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    • v.3 no.2
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    • pp.40-45
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    • 2003
  • A series of experiments has been carried out to investigate the effect of titanium, boron and nitrogen on the microstructure and toughness of simulated heat affected zone (HAZ) in quenched and tempered (QT) type 490MPa yield strength steels. For acquiring the same strength level, the carbon content and carbon equivalent could be lowered remarkably with a small titanium and boron addition due to the hardenability effect of boron during quenching process. Following the thermal cycle of large heat input, the coarsened grain HAZ (CGHAZ) of conventional quenched and tempered (QT) type 490MPa yield strength steels exhibited a coarse bainitic or ferrite side plate structure with large prior austenite grains. While, titanium and boron bearing QT type 490MPa yield strength steels were characterized by the microstructure in the CGHAZ, consisting mainly of the fine intragranular ferrite microstructure. Toughness of the simulated HAZ was mainly controlled by the proper Ceq level, and the ratio of Ti/N rather than titanium and nitrogen contents themselves. In the titanium­boron added QT steels, the optimum Ti/N ratio for excellent HAZ toughness was around 2.0, which was much lower than the known Ti/N stoichiometric ratio, 3.4. With reducing Ti/N ratio from the stoichiometric ratio, austenite grain size in the coarse grained HAZ became finer, indicating that the effective fine precipitates could be sufficiently obtained even with lower Ti/N level by adding boron simultaneously. Along with typical titanium carbo­nitrides, various forms of complex titanium­ and boron­based precipitates, like TiN­MnS­BN, were often observed in the simulated CGHAZ, which may act as stable nuclei for ferrite during cooling of weld thermal cycles

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Denitrification Characteristics and Mircoorganism Composition of Acclimatec Denitrifier Consortium

  • Park, Enu-Ju;Seo, Jae-Koan;Kim, Joong-Kyun;Suh, Kuen-Hack;Kim, Sung-Koo
    • Journal of Microbiology and Biotechnology
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    • v.10 no.3
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    • pp.410-414
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    • 2000
  • The effect of the COD/N ratio on denitrification characteristics was evaluated for the development of a denitrification process. Activated sludge, acclimated to an anoxic condition, was used as the denitrifier consortium (mixture of denitrifying organisms) for enhanced nitrogen removal in a recirculating aquarium system. Synthetic wastewater containing nitrate was used as the influent solution and glucose was used as the carbon source for denitrification. The COD/N ratio varied within a range of 1.5-7.2. The denitrification efficiency was higher than 97% even at a COD/N ratio of 1.5. Under a theoretical COD/N ratio of 3.0, nitrite was detected, however, the amount was less than 1% of the total influent nitrogen. The number of both nitrate-reducing bacteria and denitrifying bacteria reached $3.5{\times}10^5/ml$ with a COD/N ratio of 1.5 after 45 days of operation.

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Adsorption and Antibacterial Properties of Metal Treated Activated Carbon (금속 처리된 활성탄소의 흡착과 항균특성)

  • Oh, Won-Chun;Kim, Bum-Soo;Lee, Young-Hoon;Kim, Jong-Gyu;Kim, Myung-Kun;Ko, Young-Shin
    • Analytical Science and Technology
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    • v.11 no.4
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    • pp.266-270
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    • 1998
  • The Ag-treated activated carbon was prepared by the impregnation of Ag on the home made activated carbon. We investigated the nitrogen adsorption property, surface structure, and antibacterial activity of the carbon. The BET surface areas of Ag impregnated activated carbon are distributed to $740-1112.2m^2/g$ region. The results of nitrogen adsorption property show that BET surface areas move gradually to lower value with increasing $AgNO_3$ mole concentration. From the SEM results, we observed window blocking effect for micropores of external surface of adsorbent by Ag impregnation. Escherichia coli which is a kind of colon bacillus was used as bacteria for antibacterial test. From these results, we also observed that activity increase gradually to larger range with increasing $AgNO_3$ mole ratio.

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Spatio-Temporal Distribution of Particulate Organic Carbon (POC) and Nitrogen (PON) in the Southwestern Area of East Sea (동해 남서해역에서 입자성 유기탄소와 질소의 시·공간적 분포 특성)

  • Oh, Seok Jin;Jeong, Semi;Kim, Seok-Yun
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.19 no.2
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    • pp.144-150
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    • 2016
  • In southwestern East Sea, we investigated the spatio-temporal distribution characteristics of particulate organic carbon (POC) and nitrogen (PON) in September 2011 (summer), January (winter) and May 2012 (spring). Although cold waters known as the origin upwelling in the surface layer of September were not observed, this periods showed high primary productivity because of high concentrations of chlorophyll, low percentage of non-autotrophic particulate fraction among POC calculated by POC/Chl-a ratio (27%) and low POC/PON ratio (6.2), which means active amino acid and protein synthesis, However, May, 2012 showed low primary productivity because of high percentage of non-autotrophic particulate fractions among POC (66%) and high POC/PON ratio (8.1), Although spring bloom and high primary productivity has been reported in the East Sea, high percentage of non-autotrophic particulate fractions in POC, observed in the East sea during the post 2012 spring, is suggested to be due to the increase of phaeo-pigment during post spring bloom. Thus, composition of particulate organic matter may have sensitively changed by marine environmental factors in spite of same season.

Development of Organic Fertilizer based on the Cow Dung III. Studies on Tentative Guideline for Degree of Maturity (우분(牛糞)의 유기질비료화(有機質肥料化) 연구 III. 부숙도(腐熟度) 기준설정(基準設定))

  • Lim, Dong-Kyu;Jeong, Lee-Geon;Shin, Jae-Sung;Han, Ki-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.4
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    • pp.278-285
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    • 1991
  • This study was conducted to manufacture a good qualified organic fertilizer with cow dung through rapid composting process, and the proposal guideline of the degree of maturity could be estimated with the measurement of final product. It included total nitrogen content of above 2% on an oven-dry material basis, C/N ratio of below 20, CEC of more than about 60 me/100g, ratio of carbon in reducing sugar to the total carbon of below 35%, and temperature in pile of above $60^{\circ}C$. The total nitrogen content, the C/N ratio, and the Paper Chromatographic method couldn't be the guideline to evalute the maturity of cow dung compost. CEC was increased in increased fermentation and it was high in the high fermented temperature plots which were cow dung+ shredded bark in 1988, cow+dung+wood chips in 1989, and cow dung+rice straws in 1990. The ratio of carbon in reducing sugar to total carbon in 1990 was lower in cow dung+saw dust than cow dung+rice straws that was the highest temperature in pile. Generally cow dung was mixed well with saw dust and thus the total carbon of the product was high. The measurement of the temperature in pile seems to be a indirect guideline of maturity.

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Optimization of Medium Components for the Production of Crude Biosurfactant by Bacillus subtilis JK-1 (Bacillus subtilis JK-1의 생물계면활성도를 위한 최적 배지 조성)

  • Joo, Myeong-Hoon;Kim, Ji-Yeon
    • Journal of Applied Biological Chemistry
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    • v.54 no.1
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    • pp.7-14
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    • 2011
  • Bacillus subtilis JK-1 showed degradation activity against crude oil, gasoline, kerosene, and light oil, and this strain was used as a crude biosurfactant producing microorganism in this study. To optimize the culture medium for production of crude biosurfactant, the influences of various carbon, nitrogen and mineral sources were assessed. The highest biosurfactant production by B. subtilis JK-1 was observed after 96 h cultivation, containing 1.0% (w/v) soluble starch as a carbon source and 0.5% (w/v) skim milk as a nitrogen source, and carbon to nitrogen concentraion (C/N) ratio was 2.0. For the biosurfactant production 0.1% (w/v) of $KNO_3$ was the most effective mineral source. Comparison of biosurfactant production indicates that B. subtilis JK-1 produces more biosurfactant in the optimum medium established in this study than LB and TSB. Under the optimum medium, the surface tension of culture broth of B. subtilis JK-1 was decreased from 47.3 dyne/cm to 24.0 dyne/cm after cultivation of 48 h.

Polymerization and Thermal Characteristics of Acrylonitrile/Dicyclohexylammonium 2-Cyanoacrylate Copolymers for Carbon Fiber Precursors

  • Kim, Ki-Young;Park, Woo-Lee;Chung, Yong-Sik;Shin, Dong-Geun;Han, Jin-Wook
    • Carbon letters
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    • v.12 no.1
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    • pp.31-38
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    • 2011
  • This study experimentally investigated dicyclohexylammonium 2-cyanoacrylate (CA) as a potential comonomer for polyacrylonitrile (PAN) based carbon fiber precursors. The P(AN-CA) copolymers with different CA contents (0.19-0.78 mol% in the feed) were polymerized using solution polymerization with 2,2-azobis(isobutyronitrile) as an initiator. The chemical structure and composition of P(AN-CA) copolymers were determined by proton nuclear magnetic resonance and elemental analysis, and the copolymer composition was similar to the feeding ratio of the monomers. The effects of CA comonomer on the thermal properties of its copolymers were characterized differential scanning calorimetry (DSC) in nitrogen and air atmospheres. The DSC curves of P(AN-CA) under nitrogen atmosphere indicated that the initiation temperature for cyclization of nitrile groups was reduced to around $235^{\circ}C$. The heat release and the activation energy for cyclization reactions were decreased in comparison with those of PAN homopolymers. On the other hand, under air atmosphere, the P(AN-CA) with 0.78 mol% CA content showed that the initiation temperature of cyclization was significantly lowered to $160.1^{\circ}C$. The activation energy value showed 116 kJ/mol, that was smaller than that of the copolymers with 0.82 mol% of itaconic acids. The thermal stability of P(AN-CA), evidenced by thermogravimetric analyses in air atmosphere, was found higher than PAN homopolymer and similar to P(AN-IA) copolymers. Therefore, this study successfully demonstrated the great potential of P(AN-CA) copolymers as carbon fiber precursors, taking advantages of the temperature-lowering effects of CA comonomers and higher thermal stability of the CA copolymers for the stabilizing processes.

Removal of Organic and Nutrients in Fish Market Wastewater using Sequencing Batch Reactor (SBR) (SBR공정을 이용한 수산물 위판장 폐수에서 유기물 및 질소 제거)

  • Kim, Sung-Ju;Lee, Dae-Hee;Park, Hung-Suck
    • Journal of Korean Society on Water Environment
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    • v.23 no.1
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    • pp.46-51
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    • 2007
  • This research work aims at treating saline wastewater generated from a fish market using four Sequencing Batch Reactors (SBR) operated under different conditions. The effect of C/N ratio (3, 6) and salt concentration (0.5~2%) on organic and nitrogen removal was studied. The synthetic wastewater prepared with glucose ($C_6H_{12}O_6$) as the primary carbon source along with ammonium chloride ($NH_4Cl$) was used in the three reactors. The fill, anoxic, aeration, settle and draw conditions were 2 hr, 4 hr, 4 hr and 2 hr respectively. The fourth reactor was operated at different conditions to investigate the practical feasibility of SBR application to handle fish market wastewater generated in Ulsan city that had fluctuating loading characteristics. Though the unacclimated sludge was initially affected by the salt concentration, the acclimated sludge removed 95% of the organics irrespective of the NaCl concentration and C/N ratio. However, the removal of nitrogen was affected more by C/N ratio than the salt concentration. While handling fish market wastewater, though the organic and nitrogen loading rate were varying between $0.009{\sim}0.259gCOD_{OH}/gVSS/day$ and 0.005~0.034 gN/gVSS/day, the effluent concentrations were far less than the effluent standard of $120mgCOD_{OH}/L$ and 60 mgN/L respectively, except when loading rates were fluctuating and 4 times higher than the average.