• Title/Summary/Keyword: capillary model

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Quantitative Analysis of Corynomycolic Acids in Fermentation Broth

  • Jang, Ki-Hyo;Park, Yong-Il;Britz, Margaret-L.
    • Journal of Microbiology and Biotechnology
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    • v.12 no.5
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    • pp.793-800
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    • 2002
  • The mycolic acids and fatty acids of mycolic acid- containing bacteria in various types of fluids were analyzed using capillary gas chromatography and mass spectrometry. As model strains, Brevibacterium and Coryebacterium species, which have corynomycolic acids ill the range of $C_{32}C_{36}$ in the whole cell, were investigated. Optimized solvents extraction procedures for the mycolic acids and fatty acids from the culture fluids were: chloroform/methanol (1:2, v/v) as the first extraction solvents fur 4 h; and chlorofunuwater (1:1, v/v) as the second extraction solvents far 1 h. These conditions gave above 95% recovery yields fur mycolic acids from the culture fluids. The mycolic acid profile for the whole cells and the culture fluids were similar fur all the media tested. Thus, the procedure described here could be applied for the identification of mycolic acid-containing bacteria in fermentation broth or liquid from of foods.

Morphological and Rheological Studies of PMMA/Impact Modifier Blends (PMMA/충격보강제 블렌드의 몰폴로지 및 유변학적 성질에 관한 연구)

  • 오주석
    • The Korean Journal of Rheology
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    • v.9 no.4
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    • pp.151-162
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    • 1997
  • 다층 구조를 갖는 고무/아크릴 충격보강제가 poly(methyl methacrylate) (PMMA)/ 충격보강제 블렌드의 몰폴로지와 유변학적 성질에 미치는 영향을 고찰하였다. TEM으로 측 정한 블렌드 내의 충격보강제 몰폴로지는 그 함량에 관계없이 균일한 크기의 구형을 이루는 것으로 나타났으며 파괴 단면의 SEM 측정으로부터 충격보강제와 매트릭스 수지와의 강한 결합력을 확인할수있었다. 블렌드의 유변물성 측정 실험에서는 충격보강제 함량이 복합 점 도에 크게 영향을 준 반면, capillary rheometer로부터 얻은 정상상태 점도에는 별 영향을 주지않음이 확인되었다. 특히 동적응력 실험의 고진동 주파수 영역에서는 일정 충격보강제 함량이상 일 때 충격보강제 자체의 점도와 근사해졌으며 이와같은 현상을 충격보강제의 shell 분자 상호간 또는 매트릭스 분자와의 엉킴에서 비롯되는 물리적인 상호 작용측면에서 설명해보았다. 또한 실험 결과를 Palierne[16]의 비상용성 블렌드계의 이멀젼 모델과 비교한 결과 상당한 차이를 확인할수 있었으며 이러한 차이는 위에서 언급한 shell 분자 상호간 또 는 매트릭스 분자와의 엉킴에 기인한다고 추정된다.

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Phase Equilibrium of the Carbon Dioxide and Methane Hydrate in Silica Gel Pores and Thermodynamic Prediction (실리카겔 공극에서의 이산화탄소 및 메탄 하이드레이트 상평형 측정 및 열역학적 예측)

  • Kang, Seong-Pil
    • New & Renewable Energy
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    • v.3 no.2 s.10
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    • pp.47-52
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    • 2007
  • Hydrate phase equilibrium for the binary $CO_{2}$+water and $CH_{4}$+water mixtures in silica gel pore of nominal 6, 30, and 100 nm were measured and compared with the cacluated results based on van der Waals and Platteeuw model. At a specific temperature three-phase hydrate-water-vapor (HLV) equilibrium curves for pore hydrates were shifted to the higher-pressure condition depending on pore sizes when compared with those of bulk hydrates. Notably, hydrate phase equilibria for the case of 100 nominal nm pore size were nearly identical with those of bulk hydrates. The activities of water in porous silica gels were modified to account for capillary effect, and the calculation results were generally in good agreement with the experimental data.

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Numerical Analysis of Subsurface Flow in a Hillslope (자연 구릉지에서 지표하 흐름의 수치해석)

  • 최은호;남선우
    • Water for future
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    • v.24 no.1
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    • pp.109-117
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    • 1991
  • The governing equation of flow in porous media is developed on the bases of the continuity equation of fluid for transient flow through a saturated-unsaturated zone, and substitution of Darcy's law. The numerical solutions are obtained by finite element method based on the Galerkin principles weighted residuals. The analysis are carried out by using the unsteady storm data observed and rainfall intensities which are obtained by using the rainfall excess model in considering of the initial losses. The functional relationships between the hydraulic conductivity, capillary pressure head and volumetric water content are applied to the flow of water through unsaturated soil varied with changes of water content.

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Saturated - Unsaturated Transient Subsurface Flow Model on a Hillslope

  • Choi, Eun-Ho;Nahm, Sun-Woo
    • Korean Journal of Hydrosciences
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    • v.2
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    • pp.13-24
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    • 1991
  • The governing partial differential equation of flow in porous media is developed on the bases of the continuity equation of fluid for transient flow through a saturated-unsaturated zone, and substitution of Dercy's law. The numerical solution is obtained by the Galerkin finite element method based on the principle of weighted residuals. The analysis is carried out by using the unsteady storm data observed and the functional relationships between the hydraulic conductivities, capillary pressure heads, and volumetric water contents under saturated-unsaturated conditions. As the results the hydraulic conductivities, rates of change of storage and initial moisture conditions are significantly influened on the responses of subsurface flow on a hillslope.

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Pathological studies on the hepatic fibrosis induced by Capillaria hepatica (마우스에서 Capillary hepatica 감염에 의한 간섬유증의 병리학적 연구)

  • Shin, Eun-kyung;Han, Jeong-hee
    • Korean Journal of Veterinary Research
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    • v.38 no.1
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    • pp.119-128
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    • 1998
  • This study was performed to investigate the pathogenesis of hepatic granuloma and hepatic fibrosis induced in mice infected with Capillaria(C) hepatica and treated cyclophosphamide. The results were as grossly well-defined yellowish white spots and small nodules at the surface of the liver were scattered. Histopathologically, there were numerous granulomas composed of eggs and fragments of C hepatica surrounded by heavy infiltration of inflammatory cells. Severe fibrosis was observed around granulomas. Pathological lesions of group infected with C. hepatica and then injected with cyclophosphamide were most severe than those of other groups. Therefore this study suggested that hepatic fibrosis induced by C hepatica in mice would be useful for animal model of hepatic fibrosis in human.

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Mathematical Modelling of Degree of Hydration and Adiabatic Temperature Rise (콘크리트의 수화도 및 단열온도상승량 예측모델 개발)

  • 오병환;차수원;신경준;하재담;김기수
    • Proceedings of the Korea Concrete Institute Conference
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    • 1998.10b
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    • pp.883-887
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    • 1998
  • Hydration is the main reason for the growth of the material properties. A exact parameter to control the chemical and physical process is not the time, but the degree of hydration. Therefore, it is reasonable that development all material properties should be formulated in terms of degree of hydration. Mathematical formulation of degree of hydration is based on combination of reaction rate functions. The effect of moisture conditions as well as temperature on the rate of reaction is considered in the degree of hydration model. This effect is subdivided into two contributions: water shortage and water distribution. The former is associated with the effect of on the progress of hydration. The water needed for progress of hydration do not exist and there is not enough space for the reaction products to form. The latter is associated with the effect of free capillary water distribution in the pore system. Physically absorption layer does not contribute to progress of hydration and only free water is available for further hydration.

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APPLICATION OF STABLE EMULSIONS TO LIPASE IMMOBILISED MEMBRANE REACTORS FOR KINETIC RESOLUTION OF RACEMIC ESTERS

  • Giorno, Lidietta;Na, Li;Drioli, Enrico
    • Proceedings of the Membrane Society of Korea Conference
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    • 2003.07a
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    • pp.65-68
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    • 2003
  • The paper discusses the use of stable emulsion, prepared by membrane emulsification technology, to improve the enantiocatalytic performance of immobilised lipase in multiphasic membrane reactors. The production of optical pure (S)-naproxen from racemic naproxen methyl ester has been used as model reaction system. The enzyme was immobilised in the sponge layer (shell side) of capillary polyamide membrane with 50 kDa cut-off, The O/W emulsion, containing the substrate in the organic dispersed phase, was fed to the enzyme membrane reactor from shell-to-lumen. The results evidenced that lipase maintained stable activity during all the operation time (more than 250 hours), showing an enantiomeric excess (96 $\pm$2%) comparable to the free enzyme (98 $\pm$ 1%) and much higher compared to similar lipase-loaded membrane reactors used in two-separate phase systems (90%). The study showed that immobilised enzymes can achieve high stability as well as high catalytic activity and enantioselectivity.

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Separation of Colloidal Particles by Osmotic Sink Field Flow Fractionation Using UF Hollow Fiber Membranes

  • Shin, Se-Jong;Min, Byoung-Ryul;Park, Jin-Won;Ahh, Ik-Sung;Lee, Kang-Taek;Lee, Jae-Hoon
    • Korean Membrane Journal
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    • v.3 no.1
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    • pp.59-68
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    • 2001
  • Unlike existent field flow fractionation, new method, osmotic sink field flow fractionation is introduced and used ultrafiltration hollow fiber membranes as separation channel. This hollow fiber osmotic sink field flow fractionation is called HF-OSFFF. A theory that describes the retention, relaxation, resolution, plate number for the system, has been developed and experimentally verified by separation model of po1ystyrene latex beads. At external field, it is measured that radial flow rates change according to various concentrations of PEG solutions. Concentration of PEG solution vs. radial flow rate is a linear relation. For diameter distribution of unknown polymer sample, HF-OSFFF compared with the commercial capillary hydrodynamic flow fractionation (CHDF).

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An Experimental Study on the Nano-adhesion of Octadecyltrichlorosilane SAM on the Si Surface (OTS SAM의 미소 응착 특성에 관한 실험적 연구)

  • 윤의성;박지현;양승호;한흥구;공호성
    • Tribology and Lubricants
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    • v.17 no.4
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    • pp.276-282
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    • 2001
  • Nano adhesion between SPM (scanning probe microscope) tips and 075 (octadecyltrichlorosilane) SAM (self-assembled monolayer) was experimentally studied. Tests were performed to measure the nano adhesion and friction in both AFM(atomic force microscope) and LFM(lateral force microscope) modes in various conditions of relative humidity. OTS SAM was formed on Si-wafer (100) surfaces, and Si$_3$N$_4$ tips of different radius of curvature were used. When the surface was hydrophobic, the adhesion and friction forces were found lower than those of bare Si-wafer. Results also showed that micro-adhesion force increased as the relative humidity and the tip radius of curvature increased. The main parameter for affecting the micro-adhesion was found absorbed humidity on the contact surface. These results were discussed with the JKR model and a capillary force caused by absorbed water.