• Applied Biological Chemistry
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• v.38 no.5
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• pp.387-392
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• 1995

To study the regulatory expression mechanism of soybean glycinin gone, Gy2, the 5' upstream region of the gene was searched for the presence of putative regulatory elements by nucleotide sequencing. It revealed various kinds of regulatory sequence elements commonly found in plant storage protein genes. There were canonical promoter sequences, TATA box (TATAAT) and AGGA box (GAAT) which are common in the 5' upstream region of the plant genes. The embryo factor binding sequence, RY repeat, CACA sequences, ${\alpha}$-conglycinin enhancer-like sequences were also found. To delineate the function of these sequences, 5' upstream deletion mutants of Gy2 were prepared and fused to the ${\alpha}$-glucuronidase (GUS) gene. Each chimeric construct was transferred into soybean protoplasts for transient assay, which led to the identification of the sequences between -281 and -223, -170 and -122, of Gy2 promoter as negative regulatory elements, and the sequences between -223 and -170, -122 and -16 as positive regulatory elements. These results are consistent in transformed tobacco plants as well. The serially deleted promoter fragments fused to the GUS were transformed into Nicotiana tabacum by Agrobacterium tumefaciens using the binary vector system. GUS activity of Gy2 promoter deletion constructs was detected only in seeds but not in leaves with different levels of expression as in transient assay. These results suggest that the glycinin Gy2 promoter drives a tissue-specific expression in transgenic tobacco plants.

• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.4
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• pp.229-235
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• 2012

This study was conducted to investigate the climatic factors affecting bud phenology of Pinus densiflora provenances. Data were collected from Jungseon, Chungju and Jeju plantations which were parts of the 11 provenance trials established by Korea Forest Research Institute in 1996. The 36 provenances were included in this trial ($33^{\circ}30^{\prime}{\sim}38^{\circ}08^{\prime}$ in latitude and $126^{\circ}30^{\prime}{\sim}129^{\circ}20^{\prime}$ in longitude). The bud swelling date and bud burst date of the provenances were investigated from March to May in 2004 in two-day interval. The four geographic factors and fifteen climatic factors of the test sites and provenances were considered in this study. Canonical correlation analysis was conducted to examine the major factors affecting the bud phenology. Our results suggested that the major factors affecting the timing of bud swelling and burst are the differences in latitude, longitude, extremely low temperature (during December-February), extremely high temperature (during November-February) and annual mean growing days between test plantation and provenance. The provenances with lower winter temperature than that of plantation showed the faster bud swelling and bud burst. Based on these results, the implication on the seed transfer of P. densiflora was discussed.

• Korean Journal of Environment and Ecology
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• v.15 no.3
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• pp.213-223
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• 2001

This study was carried out to clarify the distribution pattern of Salicaceae species which are considered as obligatory riparian vegetation, and also the correspondence between their distribution and the environment factors. Eighty-three study sites by stratified sampling were selected from the upstream to the downstream of An-sung stream. Vegetation factors such as coverage by species, disturbance, etc., and environmental factors including microtopography, soil properties, etc., measured and analyzed. Salicaceae species were identified as total 2 genera, 11 species through all study area, and the average occurring species were 2.8 species.5. koreensis among other species showed highest occurring frequency at An-sung streams, and also it was distributed widely through study area. S. gracizistyla was mainly fecund at upstream sites, where sandy soil texture and high longitudinal slope were developed. S. purpurea vats. japonica was mostly observed in the sandy soil, the same as S. gracilistytu and however, was not dominant but rather mixed with S. gracitistyta and S. koreensis. On the other hand, distribution of S. glandulosa were closely related with littoral zone of the lake and the lower sea level with sandy loam and loamy sand Boils of high organic matter content. Under CCA, canonical correspondence analysis, distribution of Saticaceae species was positively correlated with environmental gradients such as soil properties along to topography.

• Journal of Korea Water Resources Association
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• v.42 no.3
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• pp.213-225
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• 2009

A stationary Markov chain model is a stochastic process with the Markov property. Having the Markov property means that, given the present state, future states are independent of the past states. The Markov chain model has been widely used for water resources design as a main tool. A main assumption of the stationary Markov model is that statistical properties remain the same for all times. Hence, the stationary Markov chain model basically can not consider the changes of mean or variance. In this regard, a primary objective of this study is to develop a model which is able to make use of exogenous variables. The regression based link functions are employed to dynamically update model parameters given the exogenous variables, and the model parameters are estimated by canonical correlation analysis. The proposed model is applied to daily rainfall series at Seoul station having 46 years data from 1961 to 2006. The model shows a capability to reproduce daily and seasonal characteristics simultaneously. Therefore, the proposed model can be used as a short or mid-term prediction tool if elaborate GCM forecasts are used as a predictor. Also, the nonstationary Markov chain model can be applied to climate change studies if GCM based climate change scenarios are provided as inputs.

• Journal of Ecology and Environment
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• v.29 no.6
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• pp.521-529
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• 2006

This study was carried out to classify all forest vegetation types in Ulleung Island, Korea using the methodology of the Z.-M. school's phytosociology, and map out the spatial distribution patterns of those vegetation types. The forest vegetation was classified into the mountain forest type (Acer okamotoanum community group) and maritime forest type (Artemisia scoparia community group). Vegetation units at the community level were divided into three categories; six communities, sixteen groups, and seven subgroups, giving a total of 22 communities. Total area for mapping was 5,544.9 ha, of which Fagus engleriana community accounted for 1,952 ha (35.2%), Hedera rhambea community ror 1,196 ha (21.6%), Camellia japonica community for 1,104 ha (19.9%), Sambucus sieboldiana var. pendula community for 612 ha (11.0%), Aster spathulifolius community for 506 ha (9.1%), and Tsuga sieboldii - Pinus parviflora community for 174 ha (3.1%). According to canonical correspondence analysis (CCA), variables such as $Mg^{2+}$, pH, $Ca^{2+}$ were positively correlated in maritime vegetation types, and variables such as total nitrogen, carbon content, C/N ratio and ration exchange capacity (CEC) were highly correlated in mountain vegetation types, respectively. The sea level and the slope direction were not showing regular trend as a factor to decide on species diversity, evenness and richness in this research area. But it seems to be affected by topography, slope degree and dominance vegetation.

• Korean Journal of Environmental Agriculture
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• v.27 no.1
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• pp.72-79
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• 2008

Phytosociological study was carried out to investigate the structural characteristics of Humulus japonicus community in the Middle area of Korea. The vegetation data of total 67 $relev\acute{e}s$ were analyzed by the $Z\ddot{u}rich$-Montpellier school's method. Upper layer plant communities of Humulus japonicus were classified into four community groups : Humulus japonicus-Artemisia princeps community, Chenopodium album community, Setaria viridis-Echinochloa crus-galli community and Typical community. Lower layer plant communities were classified into five community groups : Galium spurium-Stellaria aquatica community, Equisetum arvense community, Persicaria theunbergii community, Echinochloa crus-galli-Digitaria ciliaris community and Typical community. According to the results of canonical correspondence analysis by vegetation and environmental factor, Setaria viridis-Echinochloa crus-galli community of upper layer was distributed throughout the soil with high relative light and soil hardness. Chenopodium album community appeared in the soil with high pH. Galium spurium-Stellaria aquatica community of lower layer was formed in the high soil pH. Persicaria theunbergii community and Echinochloa crus-galli-Digitaria ciliaris community were distributed in the soil with high moisture.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.362-369
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• 2013

Phytoplankton and zooplankton communities were investigated from 8 weir sites of the Nakdong River system to provide basic information on plankton community after weir construction and to estimate its effects on major water quality parameters. The relationship between plankton community structure and environmental factors was analysed with CCA (Canonical Correspondence Analysis). The results suggested that discharge and total phosphorus and nitrogen concentrations are important factors determining the phytoplankton species composition. For zooplankton community, the difference in discharge between September and October induced different distribution pattern of zooplankton community with more homogeneous distribution with extreme dominance of rotifers during the high discharge season. Chlorophyll a concentration representing phytoplankton biomass has been suggested as the main environmental factor affecting zooplankton community followed by COD and total nitrogen concentration.

• Journal of the Korea institute for structural maintenance and inspection
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• v.19 no.5
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• pp.92-103
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• 2015

Box-Wilson experimental design method, known as central composite design, is the design of any information-gathering exercises where variation is present. This method was devised to gather as much data as possible in spite of the low design cost. This method was employed to model the effect of mixing factors on several performances of 60 MPa high strength self compacting concrete and to numerically calculate the optimal mix proportion. The nonlinear relations between factors and responses of HSSCC were approximated in the form of second order polynomial equation. In order to characterize five performances like compressive strength, passing ability, segregation resistance, manufacturing cost and density depending on five factors like water-binder ratio, cement content, fine aggregate percentage, fly ash content and superplasticizer content, the experiments were made at the total 52 experimental points composed of 32 factorial points, 10 axial points and 10 center points. The study results showed that Box-Wilson experimental design was really effective in designing the experiments and analyzing the relation between factor and response.

• Radiation Oncology Journal
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• v.38 no.2
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• pp.99-108
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• 2020

Purpose: The probability of recurrence of cancer after adjuvant or definitive radiotherapy in patients with human papillomavirus-negative (HPV(-)) head and neck squamous cell carcinoma (HNSCC) varies for each patient. This study aimed to identify and validate radiation sensitivity signature (RSS) of patients with HPV(-) HNSCC to predict the recurrence of cancer after radiotherapy. Materials and Methods: Clonogenic survival assays were performed to assess radiosensitivity in 14 HNSCC cell lines. We identified genes closely correlated with radiosensitivity and validated them in The Cancer Genome Atlas (TCGA) cohort. The validated RSS were analyzed by ingenuity pathway analysis (IPA) to identify canonical pathways, upstream regulators, diseases and functions, and gene networks related to radiosensitive genes in HPV(-) HNSCC. Results: The survival fraction of 14 HNSCC cell lines after exposure to 2 Gy of radiation ranged from 48% to 72%. Six genes were positively correlated and 35 genes were negatively correlated with radioresistance, respectively. RSS was validated in the HPV(-) TCGA HNSCC cohort (n = 203), and recurrence-free survival (RFS) rate was found to be significantly lower in the radioresistant group than in the radiosensitive group (p = 0.035). Cell death and survival, cell-to-cell signaling, and cellular movement were significantly enriched in RSS, and RSSs were highly correlated with each other. Conclusion: We derived a HPV(-) HNSCC-specific RSS and validated it in an independent cohort. The outcome of adjuvant or definitive radiotherapy in HPV(-) patients with HNSCC can be predicted by analyzing their RSS, which might help in establishing a personalized therapeutic plan.

• Korean Journal of Ichthyology
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• v.30 no.4
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• pp.224-231
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• 2018

Dietary niches can support the coexistence of sympatric species in marine ecosystems, which can lead to the presence of greater abundances of those species. Stomach contents of silver croaker (Pennahia argentata) and Japanese sillago (Sillago japonica) inhabiting the Nakdong River estuary, Korea were analyzed to determine diet compositions and the presence of any size-related and inter-specific feeding relationships. These species were bottom-feeding predators that consumed mainly benthic crustaceans and mollusks, but polychaetes were also important in the S. japonica diets. Non-metric multidimensional scaling (nMDS) ordination and multivariate analyses based on weight contributions of the different prey taxa to stomach contents revealed significant inter-specific dietary differences. Size-related change was also significant for P. argentata diets, but not for S. japonica. Canonical analysis of principal coordinate (CAP) ordination plot further demonstrated that differences in the type and range of prey ingested by the two species contributed such an inter-specific difference in the diet compositions. The results from this study provide clear evidence of niche segregation between co-occurring P. argentata and S. japonica in the Nakdong River estuary, which would reduce the likelihood of inter-specific competition for food resources.