• Title/Summary/Keyword: canonical analysis

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Disinfection of E. coli Using Electro-UV Complex Process: Disinfection Characteristics and Optimization by the Design of Experiment Based on the Box-Behnken Technique (전기-UV 복합 공정을 이용한 E. coli 소독 : 실험계획법중 박스-벤켄법을 이용한 소독 특성 및 최적화)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.19 no.7
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    • pp.889-900
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    • 2010
  • The experimental design and response surface methodology (RSM) have been applied to the investigation of the electro-UV complex process for the disinfection of E. coli in the water. The disinfection reactions of electro-UV process were mathematically described as a function of parameters power ($X_1$), NaCl dosage ($X_2$), initial pH ($X_3$) and disinfection time ($X_4$) being modeled by use of the Box-Behnken technique. The application of RSM using the Box-Behnken technique yielded the following regression equation, which is an empirical relationship between the residual E. coli number and test variables in actual variables: Ln (CFU) = 23.57 - 0.87 power - 1.87 NaCl dosage - 2.13 pH - 2.84 time - 0.09 power time - 0.07 NaCl dosage pH + 0.14 pH time + 0.03 $power^2$ + 0.47 NaCl $dosage^2$ + 0.20 $pH^2$+ 0.33 $time^2$. The model predictions agreed well with the experimentally observed result ($R^2$ = 0.9987). Graphical response surface and contour plots were used to locate the optimum point. The estimated ridge of maximum response and optimal conditions for the E. coli disinfection using canonical analysis was Ln 1.06 CFU (power, 15.40 W; NaCl dosage, 1.95 g/L, pH, 5.94 and time, 4.67 min). To confirm this optimum condition, the obtained number of the residual E. coli after three additional experiments were Ln 1.05, 1.10 and Ln 1.12. These values were within range of 0.62 (95% PI low)~1.50 (95% PI high), which indicated that conforming the reproducibility of the model.

Environmental Factors Influencing Tree Species Regeneration in Different Forest Stands Growing on a Limestone Hill in Phrae Province, Northern Thailand

  • Asanok, Lamthai;Marod, Dokrak
    • Journal of Forest and Environmental Science
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    • v.32 no.3
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    • pp.237-252
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    • 2016
  • Improved knowledge of the environmental factors affecting the natural regeneration of tree species in limestone forest is urgently required for species conservation. We examined the environmental factors and tree species characteristics that are important for colonization in diverse forest stands growing on a limestone hill in northern Thailand. Our analysis estimated the relative influence of forest structure and environmental factors on the regeneration traits of tree species. We established sixty-four $100-m^2$ plots in four forest stands on the limestone hill. We determined the species composition of canopy trees, regenerating seedlings, and saplings in relation to the physical environment. The relationships between environmental variables and tree species abundance were assessed by canonical correspondence analysis (CCA), and we used generalized linear mixed models to examine data on seedling/sapling abundances. The CCA ordination indicated that the abundance of tree species within the mixed deciduous forest was closely related to soil depth. The abundances of tree species growing within the sink-hole and hill-slope stands were positively related to the extent of rocky outcropping; light and soil moisture positively influenced the abundance of tree species in the hill-cliff stand. Physical factors had a greater effect on tree regeneration than did factors related to forest structure. Tree species, such as Ficus macleilandii, Dracaena cochinchinensis, and Phyllanthus mirabilis within the hill-cliff or sink-hole stand, colonized well on large rocky outcroppings that were well illuminated and had soft soils. These species regenerated well under conditions prevailing on the limestone hill. The colonization of several species in other stands was negatively influenced by environmental conditions at these sites. We found that natural regeneration of tree species on the limestone hill was difficult because of the prevailing combination of physical and biological factors. The influence of these factors was species dependent, and the magnitude of effects varied across forest stands.

Seasonal Variation in Species Composition of the Larval Fish in the Han River Estuary, Korea (한강 하구역 자치어 종조성의 계절 변동)

  • Kim, Ji Hye;Kim, Byung Gi;Han, Kyung Nam
    • Korean Journal of Ichthyology
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    • v.26 no.2
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    • pp.125-132
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    • 2014
  • Seasonal variation and abundance of fish larvae in Han River estuary were determined by analysis of seasonal samples collected from three channels in the Han River estuary from May 2007 to August 2008. During the study, a total of 3,642 fish larvae were collected belonging to 13 taxa. Coilia spp. was the most abundant (48.8%). The highest number of taxa (11 taxa) was collected in the Jangbong channel and abundance was high in the Sukmo channel (2,428 ind./$1,000m^3$). Brackish-water species were prevailed in the Yumhwa channel and the Sukmo channel, while coastal species were abundant in the Jangbong channel. Canonical correspondence analysis revealed that the environmental factors such as water temperature and salinity had a strong relationship with the abundance of major larvae species.

The Arabidopsis Phytocystatin AtCYS5 Enhances Seed Germination and Seedling Growth under Heat Stress Conditions

  • Song, Chieun;Kim, Taeyoon;Chung, Woo Sik;Lim, Chae Oh
    • Molecules and Cells
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    • v.40 no.8
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    • pp.577-586
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    • 2017
  • Phytocystatins (PhyCYSs) are plant-specific proteinaceous inhibitors that are implicated in protein turnover and stress responses. Here, we characterized a PhyCYS from Arabidopsis thaliana, which was designated AtCYS5. RT-qPCR analysis showed that the expression of AtCYS5 in germinating seeds was induced by heat stress (HS) and exogenous abscisic acid (ABA) treatment. Analysis of the expression of the ${\beta}-glucuronidase$ reporter gene under the control of the AtCYS5 promoter showed that AtCYS5 expression during seed germination was induced by HS and ABA. Constitutive overexpression of AtCYS5 driven by the cauliflower mosaic virus 35S promoter led to enhanced HS tolerance in transgenic Arabidopsis, which was characterized by higher fresh weight and root length compared to wild-type (WT) and knockout (cys5) plants grown under HS conditions. The HS tolerance of AtCYS5-overexpressing transgenic plants was associated with increased insensitivity to exogenous ABA during both seed germination and post-germination compared to WT and cys5. Although no HS elements were identified in the 5'-flanking region of AtCYS5, canonical ABA-responsive elements (ABREs) were detected. AtCYS5 was upregulated in ABAtreated protoplasts transiently co-expressing this gene and genes encoding bZIP ABRE-binding factors (ABFs and AREB3). In the absence of ABA, ABF1 and ABF3 directly bound to the ABREs in the AtCYS5 promoter, which activated the transcription of this gene in the presence of ABA. These results suggest that an ABA-dependent pathway plays a positive role in the HS-responsive expression of AtCYS5 during seed germination and post-germination growth.

Effect of online word-of-mouth variables as predictors of box office (영화 흥행 예측변수로서 온라인 구전 변수의 효과)

  • Jeon, Seonghyeon;Son, Young Sook
    • The Korean Journal of Applied Statistics
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    • v.29 no.4
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    • pp.657-678
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    • 2016
  • This study deals with the effect of online word-of-mouth (OWOM) variables on the box office. From the result of statistical analysis on 276 films with audiences of more than five hundred thousand released in the Korea from 2012 to 2015, it can be seen that the variables showing the size of OWOM (such as the number of the portal movie rater, blog, and news after release) are associated more with the box office than the portal movie rating showing the direction of OWOM as well as variables showing the inherent properties of the film such as grade, nationality, release month, release season, directors, actors, and distributors.

A Comparative Study of Consumption Propensity and Determinants of Purchasing According to Cultural Differences between Baby Boomers and Millennials: Application to Franchise Customers

  • Roh, Hyun-Sik;Lee, Sung-Ho;Kim, Sung-Hoon
    • The Korean Journal of Franchise Management
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    • v.11 no.1
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    • pp.31-44
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    • 2020
  • Purpose: The baby boomers and the millennials, who make up the largest share of the market, are showing the gap in their differentiated cultural styles. The purpose of this study is to study the consumption propensity and purchasing determinants of the baby boomers generation and the millennials generation, which have become a very important key pillar in shaping the market based on the cultural dimension model theory of h(=>H)opstead, and to identify the difference Research design, data, and methodology: In order to verify the research hypotheses, The data was collected from the baby boomers and the millennials using online questionnaires. The pre-test was conducted from October 10 to 12, 2019, and the main survey was conducted from October 15 to 25. A total of 230 copies were recalled, and the final 219 copies were used for the final analysis. Results: The consumption propensity between the baby boomers and the millennials differed in resource saving purchase propensity and impulse buying propensity. The baby boomers showed higher resource saving purchase propensity than the millennials, while the millennials showed higher impulse buying propensity. There was a difference in the determinants to purchasing only in the evaluation factor. Results of gender differences by generation, baby boomers differed in their planned purchase propensity, and women were higher. The millennials generation differed in resource saving purchase propensity and awareness propensity for others, all higher in men. In determinants to purchasing, the baby boomers did not differ in gender, and the millennials did differ in product factors. A canonical correlations analysis of the relationship between the baby boomers and the millennials showed a significant relationship between consumption propensity and determinants to purchasing. Also, the baby boomer generation has the biggest link between impulse buying propensity and evaluation factor. The millennials generation showed the biggest link between resource saving purchase and product factors. Conclusions: This study compared consumption propensity and determinants to purchasing of baby boomers and millennials on the basis of Hopstead's cultural dimension model theory to identify differences between generations, presenting practical and theoretical implications for establishing a correct understanding and specific marketing strategy among generation.

Spatial distribution of halophytes and environment factors in salt marshes along the eastern Yellow Sea

  • Chung, Jaesang;Kim, Jae Hyun;Lee, Eun Ju
    • Journal of Ecology and Environment
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    • v.45 no.4
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    • pp.264-276
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    • 2021
  • Background: Salt marshes provide a variety of ecosystem services; however, they are vulnerable to human activity, water level fluctuations, and climate change. Analyses of the relationships between plant communities and environmental conditions in salt marshes are expected to provide useful information for the prediction of changes during climate change. In this study, relationships between the current vegetation structure and environmental factors were evaluated in the tidal flat at the southern tip of Ganghwa, Korea, where salt marshes are well-developed. Results: The vegetation structure in Ganghwa salt marshes was divided into three groups by cluster analysis: group A, dominated by Phragmites communis; group B, dominated by Suaeda japonica; and group C, dominated by other taxa. As determined by PERMANOVA, the groups showed significant differences with respect to altitude, soil moisture, soil organic matter, salinity, sand, clay, and silt ratios. A canonical correspondence analysis based on the percent cover of each species in the quadrats showed that the proportion of sand increased as the altitude increased and S. japonica appeared in soil with a relatively high silt proportion, while P. communis was distributed in soil with low salinity. Conclusions: The distributions of three halophyte groups differed depending on the altitude, soil moisture, salinity, and soil organic matter, sand, silt, and clay contents. Pioneer species, such as S. japonica, appeared in soil with a relatively high silt content. The P. communis community survived under a wider range of soil textures than previously reported in the literature; the species was distributed in soils with relatively low salinity, with a range expansion toward the sea in areas with freshwater influx. The observed spatial distribution patterns may provide a basis for conservation under declining salt marshes.

The Regulation of LexA on UV-Induced SOS Response in Myxococcus xanthus Based on Transcriptome Analysis

  • Sheng, Duo-hong;Wang, Ye;Wu, Shu-ge;Duan, Rui-qin;Li, Yue-zhong
    • Journal of Microbiology and Biotechnology
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    • v.31 no.7
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    • pp.912-920
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    • 2021
  • SOS response is a conserved response to DNA damage in prokaryotes and is negatively regulated by LexA protein, which recognizes specifically an "SOS-box" motif present in the promoter region of SOS genes. Myxococcus xanthus DK1622 possesses a lexA gene, and while the deletion of lexA had no significant effect on either bacterial morphology, UV-C resistance, or sporulation, it did delay growth. UV-C radiation resulted in 651 upregulated genes in M. xanthus, including the typical SOS genes lexA, recA, uvrA, recN and so on, mostly enriched in the pathways of DNA replication and repair, secondary metabolism, and signal transduction. The UV-irradiated lexA mutant also showed the induced expression of SOS genes and these SOS genes enriched into a similar pathway profile to that of wild-type strain. Without irradiation treatment, the absence of LexA enhanced the expression of 122 genes that were not enriched in any pathway. Further analysis of the promoter sequence revealed that in the 122 genes, only the promoters of recA2, lexA and an operon composed of three genes (pafB, pafC and cyaA) had SOS box sequence to which the LexA protein is bound directly. These results update our current understanding of SOS response in M. xanthus and show that UV induces more genes involved in secondary metabolism and signal transduction in addition to DNA replication and repair; and while the canonical LexA-dependent regulation on SOS response has shrunk, only 5 SOS genes are directly repressed by LexA.

Prediction Modeling through Quantification for Qualitative Variables (질적변수에 대한 계량화를 통한 사면붕괴 예측모형)

  • Na, Jong-Hwa;Yu, Hye-Kyung;Nam, Eun-Mi;Cho, Wan-Sup
    • Journal of Korea Society of Industrial Information Systems
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    • v.14 no.5
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    • pp.281-288
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    • 2009
  • The purpose of this paper is to provide the statistical models for landslide prediction through quantification and AHP methods. Quantification method is a statistical method of providing quantity to qualitative variables by analyzing the observed data. In this paper, we suggest the quantification process based on the results of cannonical correlation analysis. In contrast with the quantification method which is based on given data the AHP(Analytic Hierarchy Process) technique is a kind of method based on questionaire data which is usually taken from professionals. We analyze both the real data(provided from KIGAM) and questionaire data collected from professionals of various related area. We developed two kinds of evaluation table which provide the scores of land slide possibility and the logistic model providing the probability of occurring landslide. Finally we compare the performance and evaluate the stability of the suggested two models.

Analysis of Characteristics and Optimization of Photo-degradation condition of Reactive Orange 16 Using a Box-Behnken Method (실험계획법 중 Box-Behnken(박스-벤켄)법을 이용한 반응성 염료의 광촉매 산화조건 특성 해석 및 최적화)

  • Cho, Il-Hyoung;Lee, Nae-Hyun;Chang, Soon-Woong;An, Sang-Woo;Yonn, Young-Han;Zoh, Kyung-Duk
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.9
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    • pp.917-925
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    • 2006
  • The aim of our research was to apply experimental design methodology in the optimization of photocatalytic degradation of azo dye(Reactive orange 16). The reactions were mathematically described as a function of parameters amount of $TiO_2(x_1)$, and dye concentration($x_2$) being modeled by the use of the Box-Behnken method. The results show that the responses of color removal(%)($Y_1$) in photocatalysis of dyes were significantly affected by the synergistic effect of linear term of $TiO_2(x_1)$ and dye concentration($x_2$). Significant factors and synergistic effects for the $COD_{Cr}$, removal(%)($Y_2$) were the linear term of $TiO_2(x_1)$ and dye concentration($x_2$). However, the quadratic term of $TiO_2(x_1^2)$ and dye concentration($x_2^2$) had an antagonistic effect on $Y_1$ and $Y_2$ responses. Canonical analysis indicates that the stationary point was a saddle point for $Y_1$ and $Y_2$, respectively. The estimated ridge of maximum responses and optimal conditions for $Y_1:(X_1,\;X_2)$=(1.11 g/L, 51.2 mg/L) and $Y_2:(X_1,\;X_2)$=(1.42 g/L, 72.83 mg/L) using canonical analysis was 93% and 73%, respectively.