• Plant Resources
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• 제4권1호
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• pp.53-58
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• 2001

The effect of carbenicillin on the dedifferentiation and the regeneration efficiency of plant tissues of horseradish(Armoracia rusticana) was evaluated, Inhibition effect for callus initiation was observed when leaf blade, root and petiole segments were grown on MS medium containing 500 mg/L to 2000 mg/L carbenicillin and 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The regeneration of horseradish shoots from leaf blade, root and petiole explants were decreased as the addition of carbenicillin increased from 1000 mg/L to 2000 mg/L in MS medium containing 0.5 mg/L of 6-benzylaminopurine (BAP) or kinetin. Especially, 500 mg/L carbenicillin treatment significantly inhibited shoot induction when leaf blade explants were grown on hormone-free MS medium. It was suggested that the toxic effects of combinations of carbenicillin and 2,4-D may be due to high auxin activity levels.

• Journal of Forest and Environmental Science
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• 제39권3호
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• pp.150-154
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• 2023

Heloniopsis orientalis (Liliaceae) is an important horticultural crop native to Korea. Under natural conditions, germination is poor and plant growth is delayed. Therefore, we have developed a vegetative propagation method to produce plants with vigorous growth characteristics via tissue culture. The regenerated shoots were then initiated directly from leaf explants on an MS medium containing either 0.5 to 2.0 mg/L 2,4-D or 1.0 to 3.0 mg/L BA. Healthy plantlets with adventitious roots were formed on the medium supplemented with 1.0 mg/L BA (81%). BA triggered callus initiation without caulogenesis or rhizogenesis, and callus formation was better on the half-strength MS medium than on the full-strength medium. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

• Journal of Plant Biotechnology
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• 제30권1호
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• pp.47-52
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• 2003

본 연구는 당근에 있어서 유용한 육성재료를 순계화하기 위한 가능성을 찾기 위하여 약배양을 수행하였다. 캘러스를 유도하기 위하여 여러 계통의 약을 1.0 and 2.0 mg/L 2,4-D와 NAA가 첨가된 B$_{5}$ 반고체배지에서 배양하였다. 2,4-D가 함유된 배지가 NAA가 함유된 배지보다 캘러스 형성에 큰 영향을 미치는 것으로 나타났다. 특히 계통 45477의 경우 1.0 mg/L 2,4-D가 함유된 배지에서 약 50%의 높은 캘러스 형성률을 보였다. 두 달 정도 배양하면 충분히 캘러스를 유기할 수 있었다. BAP 또는 NAA 농도가 낮아질수록 식물체 생산에 효과적이라는 것을 알 수가 있었으며 식물생장조절물질이 전혀 첨가되지 않은 배지에서 캘러스를 배양하는 것이 기내에서 식물체 생산에 이상적이었다. 노랗고 부드러운 조직을 보이는 캘러스에서 식물체 분화율이 높게 나타났으며 약배양에 대한 반응은 계통간에 뚜렷한 차이를 보였다. 배지에 함유된 생장 조절물질을 조절함으로써 약으로부터 캘러스를 유기하거나 식물체로 분화시킬 수 있었다. 배양묘의 엽수에 관계없이 퍼얼라이트 배지에서 4주간 충분히 순화시킨 유식물체에서 포장 생존율이 100%로 가장 높았다. 지금까지의 연구 결과 한개의 캘러스에서 식물체를 대량으로 생산할 수 있었으며 이약배양기법이 당근 품종육성에 이용될 것으로 전망된다.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.239-244
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• 2003

A protocol has been developed for rapid large scale clonal propagation of an aromatic endangered medicinal plant, Hemidesmus indicus R. Br. with the elimination of the problems such as premature leaf fall and callus formation during caulogenesis and rhizogenesis. Multiple shoots were induced from shoot tip and nodal explants on Murashige and Skoog (MS) medium supplemented with 1 mg/L Benzylaminopurine (BAP) and 0.5 mg/L Napthaleneaceticacid (NAA). Addition of 15 mg/L adenine sulphate to the above medium checked leaf abscission completely, reduced the time required for caulogenesis and restored morphogenetic potential after several subcultures. The in vitro grown propagules were rooted in 1/2 MS medium supplemented with 2 mg/L Indolebutyric acid (IBA) +1 mg/L NAA and sucrose 0.7% (w/v). Addition of charcoal at 100 mg/L to the rooting medium quickened root initiation with a complete check on callus formation. The effect of sucrose concentration on both caulogenesis and rhizogenesis was also studied. The resultant plantlets were acclimatized and grown in fields where ninety eight percent of the rooted shoots survived and grew normally. The estimation of the secondary metabolite content in the shoots of the regenerated plant and the mother plant indicated that the concentration of the three secondary metabolites lupeol, vanillin and rutin was similar.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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• pp.2-3
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• 2003

A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot initiation from nodal segments was very rare. Mature apices produced callus. Although removal of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.

• 한국초지조사료학회지
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• 제21권1호
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• pp.35-38
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• 2001

버즈풋 트레포일 (Lotus corniculatus L.)의 줄기부분을 절단하여, SH 배지에 IBA를 첨가한 농도별로 뿌리 유도 정도를 조사한 결과. IBA 농도가 높을수록 뿌리 유도가 좋았으며, 2.5 mg/$\ell$의 IBA를 첨가한 처리구에서 뿌리 유도 정도가 90~95%로서 가장 좋았다. 캘러스로부터 shoot를 유도한 다음 줄기를 취하여 처리한 경우에는, 처리 12일째에 1/2 SH-0 배지에서 9개 (45%), SH-0 배지에서 10 (50%), SH-0.5IBA 배지에서 10개 (50%), SH-1.0IBA 배지에서 10개 (50%), SH-1.5IBA 배지에서 13개 (65%), SH-2.0IBA 배지에서 14개 (70%), SH-2.5IBA 배지에서 19개 (95%). SH-3.0IBA 배지에서 14개 (70%)의 뿌리유도를 나타내었다.

• 한국초지조사료학회지
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• 제21권1호
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• pp.27-30
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• 2001

알팔파 (Medicago sativa L.)의 줄기부분을 절단하여, SH 배지에 IBA를 첨가한 농도별로 뿌리 유도 정도를 조사한 결과, IBA 농도를 1.0~1.5 mg/$\ell$로 첨가한 처리구에서 뿌리 유도 정도가 70~75%로서 가장 좋았다. 캘러스로부터 shoot를 유도한 다음 줄기를 취하여 처리한 경우에는. 처리 10일째에 1/2 SH-0 배지에서 10개 (50%), SH-0 배지에서 10 (50%), SH-0.5IBA 배지에서 12개 (60%), SH-1.0IBA 배지에서 15개 (75%), SH-1.5IBA 배지에서 15개 (75%), SH-2.0IBA 배지에서 10개 (50%), SH-2.5IBA 배지에서 9개 (45%), SH-3.0IBA 배지에서 8개 (40%)의 뿌리 유도를 나타내었다.

• Journal of Ginseng Research
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• 제10권1호
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• pp.45-54
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• 1986

인삼에 이용할 수 있는 vector system의 개발연구의 일환으로 우선 Agrobacterium spp.를 인삼의 잎, 줄기 및 뿌리에 접종하여 crown gall tumor의 형성 및 탈분화 그리고 Agrobacterium spp.의 opine화합물의 이용정도등을 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. Agrobacterium tumefaciens C58은 인삼의 모든 부위에서 crown gall tumor를 형성하였으나 secondary tumor나 teratoma는 형성하지 못했다. 2. Wild type Agrobacterium tumefaciens Y101, Y104, Y109는 crown gall tumor를 형성하였으며, tumor의 형태, 크기 그리고 생장 정도는 strain별로 차이가 있었다. 3. Agrobacterium tumefaciens Y194는 특히 amorphic tumor를 형성하였다. 4. 줄기에서 형성된 tumor조직에서 callus를 유기하고자 phytohormone free배지 및 2,4-D 첨가 배지에 접종한 결과 전혀 callus가 형성되지 않았다. 5. 뿌리에서 형성된 callus가 형성되긴 하였으나 출현빈도가 극히 낮았으며, 정상 조직과는 달리 2,4-D의 효과가 미미하였다. 6. Agrobacterium spp.에 의한 opone화합물의 이용능력을 조사한 결과, Agrobacterium tumefacciens Y104, Y110 과 C58은 nopaline type이었고 Y109는 octopine type이었으며, Y101은 nopaline과 octopine 어느것도 이용하지 못하였다.

• Journal of Crop Science and Biotechnology
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• 제10권2호
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• pp.92-97
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• 2007

Antibiotics used for suppressing Agrobacterium in plant transformation procedure might have negligible effects on plant tissues and regeneration. The effects of antibiotics on growth suppression of Agrobacterium and plant regeneration were investigated for enhancing Agrobacterium-mediated transformation using wheat mature embryos. Antibiotics tested, except carbenicillin, were able to suppress that embryos were coated with a layer of Agrobacterium cells in callus induction medium. Agrobacterium growth was suppressed minimally at 50 mg/l of timentin, while cefotaxime and clavamox were completely suppressed at relative high concentration of 250 mg/l. In the treatment of carbenicillin, initiation of growth suppression of Agrobacterium occurred at 750 mg/l of concentration because Agrobacterium KYRT1 contains the carbenicillin resistant gene. In Agrobacterium inoculation, effects of antibiotics were significantly different on the rate of callus induction and shoot formation. Almost embryos were induced calli at 50 mg/l of timentin whereas callus induction rate was achieved above 90% at 100 mg/l and 250 mg/l of cefotaxime and clavamox, respectively. Shoot formation rate was higher in the treatment of timentin than that of cefotaxime and clavamox at 500 mg/l of concentration, respectively. Timentin can be used as a good antibiotics in Agrobacterium-mediated wheat transformation.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 심포지엄
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• pp.9-18
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• 2003

A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot idtiation from nodal segments was very rare. Mature apices produced callus. Although removed of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.