• 구강회복응용과학지
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• 제25권3호
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• pp.279-285
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• 2009

본 연구의 목적은 인산칼슘제제인 $MBCP^{(R)}$, $Polybone^{(R)}$을 발치와에 이식 후 치유 양상을 조직학적으로 관찰하는 것이다. 비글견의 소구치를 발치 후 발치와에 골이식재를 이식하였으며, 3개월간의 치유기간을 거친 후 조직표본을 제작하여 현미경으로 치유 양상을 관찰하였다. $MBCP^{(R)}$, $Polybone^{(R)}$은 조직학적으로 비슷한 치유 양상을 보였으며 이물반응이나 염증 반응 없이 신생골 생성을 유도하여 정상적인 치유를 도모하였다. 치유된 조직의 조직 계측학적 평가에서 신생골 비율은 골이식재를 이식하지 않은 대조군이 34.5%, $MBCP^{(R)}$는 28.4%, $Polybone^{(R)}$은 23.8%였으며, 골이식재를 포함한 전체 mineralized tissue의 비율은 각각 34.5%, 42.9%, 37.3% 였다. 이상의 결과에서 $MBCP^{(R)}$, $Polybone^{(R)}$은 발치와에 사용할 골이식재로 가치가 있는 것으로 생각된다.

• 한국식품조리과학회지
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• 제18권3호
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• pp.349-354
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• 2002

산성조미료로서 이용될 뿐만 아니라 여러 가지 조리 및 영양생리작용을 가진 곡물식초와 구연산을 사골뼈에 이용하여 조리할 때 산의 종류가 뼈로부터 칼슘, 인, 마그네슘, 아미노산, 콜라겐의 용출에 미치는 영향을 조사하였다. 그 결과 칼슘, 인, 마그네슘 그리고 아미노산의 용출량은 0, 0.5, 1, 2% 순으로 산농도가 높을수록 증가하였다. 그리고 같은 산농도에서 구연산 첨가군이 곡물식초 첨가군보다 많이 용출되었다. 칼슘과 인의 비율에서 곡물식초 첨가군과 구연산 첨가군 모두 칼슘이 인보다 많이 용출되었다. 곡물식초와 구연산 농도 2%를 첨가하여 12시간 끓인 시료를 이용하여 탕수두부를 만들어 관능검사를 실시한 결과 신맛, 맛, 종합적인 평가에서 대조군과 곡물식초 첨가군이 구연산 첨가군 보다 높은 기호도를 나타내었다. 이상의 결과들로부터 사골뼈에 산성조미료를 첨가하여 12시간 가열함으로써 칼슘과 인은 가용화되기 쉬우며, 관능검사 결과 소스로서의 다양한 이용과 개발을 시사하였다.

• Annals of Laboratory Medicine
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• 제38권6호
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• pp.518-523
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• 2018

Background: Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Methods: Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation ($108,200{\times}g$) and high-speed centrifugation ($10,000{\times}g$ for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods-LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). Results: When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. Conclusions: High-speed centrifugation ($10,000{\times}g$ for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.

• 대한두개안면성형외과학회지
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• 제22권5호
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• pp.239-246
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• 2021

Background: Bone grafts can provide an optimal environment for permanent tooth to erupt and enhance the stability of the alveolar maxilla. Although autologous bone is an optimal source for osteogenesis, its inevitable donor site morbidity has led to active research on bone substitutes. This study was designed to evaluate the safety and feasibility of using biphasic calcium phosphate (BCP; Osteon) as a bone substitute in dogs. Methods: Bilateral third and fourth premolars of four 15-week-old mongrel dogs were used. All teeth were extracted except the third premolar of the right mandible, which was used as a control. After extraction of the premolars, each dog was administered BCP (Osteon), demineralized bone matrix (DBM; DBX), and no graft in the hollow sockets of the right fourth premolar, left fourth premolar, and left third premolar, respectively. Radiographs were taken at 2-week intervals to check for tooth eruption. After 8 weeks, each dog was sacrificed, and tooth and bone biopsies were performed to check for the presence of tooth and bone substitute particle remnants. Results: Four weeks after the operation, permanent tooth eruptions had started at all the extraction sites in each dog. Eight weeks after the operation, all teeth had normally erupted, and histological examination revealed BCP particles at the right fourth premolar. Conclusion: In all four dogs, no delay in the eruption of the teeth or shape disfigurement of permanent teeth was observed on gross inspection and radiologic evaluation. On histological examination, most of the BCP and DBM were replaced by new bone. Bone substitutes can be used as graft materials in patients with alveolar clefts.

• 한국응용과학기술학회지
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• 제34권2호
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• pp.289-295
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• 2017

현 국제해사기구(IMO)에서의 선박엔진에서 발생되는 NOx와 SOx 등의 연소 가스 배출에 대한 규제 강화에 따라, 발트 해 연안을 지나는 모든 선박들은 배출되는 연소가스 저감장치를 장착해야 된다. 국내에서도 IMO의 규제에 따른 NOx와 SOx를 저감장치를 개발하고 있으며, 그중에 대표적인 장치인 Scrubber는 세정액으로 암모니아수와 요소수를 사용하게 되고 사용된 폐 세정액에는 NOx와 SOx와 반응한 질산암모늄과 황산암모늄이 포함되어 있다. 본 연구에서는 폐 세정액이 포함하고 있는 유용한 부산물을 유기용매를 사용하는 염석법을 적용하여 회수하였다. 질산암모늄과 황산암모늄의 회수방법과 질안석회를 추출 후 회수된 부산물의 정성분석을 위하여, FT-IR 분석을 통하여 물질의 정성적 특성과 화학적 조성을 평가해 보았다. 한편 응집제를 투입하여 질안석회를 침전시켜 비료상의 물질로 회수하였다. $FeSO_4$ 응집제와 $CaCl_2$를 응집보조제로 사용하고 입자의 크기를 키우기 위해 $CaCO_3$를 사용하였다.

• Journal of Applied Biological Chemistry
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• 제60권1호
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• pp.19-22
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• 2017

사골로부터 칼슘과 인의 용출량을 증가시킴과 동시에 칼슘의 흡수에 효과적인 비율로 칼슘과 인의 함량을 조절하기 위하여 초산과 구연산의 혼합효과를 조사하였다. 사골에 구연산과 초산을 각각 첨가하여 추출물을 제조한 결과, 구연산은 사골로부터 칼슘과 인을 유사하게 용출시키나, 초산은 인보다 칼슘을 선택적으로 용출시켰다. 초산 0.2 %와 구연산 0.1 %를 혼합하여 제조한 추출물은 초산 0.2 %의 결과에 비하여 칼슘의 용출량은 약 30 %($26.89{\rightarrow}34.22mg$) 증가하였으나 인의 용출량은 약 400 % ($3.57{\rightarrow}18.45mg$) 증가하였으며, 칼슘과 인의 비율은 7.53에서 1.85로 크게 개선되었다. 관능평가에서 맛과 전체적인 기호도는 0.2 % 초산+0.1 % 구연산을 첨가하여 제조한 시료가 증류수와 0.2 % 초산을 사용한 시료보다 우수하였다.

• 한국토양환경학회지
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• 제2권2호
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• pp.73-79
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• 1997

알칼리용출법을 사용하여 폐광산 광미로부터 비소성분을 제거하기 위한 실험을 실시하였다. 대상시료는 오래전에 폐광된 폐광산인 다덕광산 및 유천광산에서 채취한 광미를 사용하였으며, 알칼리용출액으로는 가성소다 수용액을 사용하였다. 알칼리용출후 용액중의 비소 성분은 칼슘비소화합물 형태의 불용성 침전물로 전환시키는 방법으로 제거할수 있었다. 실험결과 침출조건에 따라 약 60∼90%의 비소침출율을 달성할 수 있었으며, 0.5N이상의 가성소다 농도에서는 침출율에 대한 온도 및 슬러리 농도의 영향이 매우 작은 것으로 나타났다 또한, 1회 용출시 소모되는 알칼리 양이 별로 크지 않기 때문에 0.5N이상의 가성소다 용액을 사용할 경우 용출액 재사용이 가능한 것으로 나타났다. 한편, 비소 침전실험 결과 침출액에 대하여 2wt% 염화칼슘을 첨가함으로써 침전시간 10분이내에 99%이상의 비소 침전율을 얻을 수 있었다.

• Journal of Periodontal and Implant Science
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• 제50권6호
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• pp.418-434
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• 2020

Purpose: The purpose of the present study was to evaluate the effect of silica-calcium phosphate composite (SCPC) granules on bone regeneration in extraction sockets. Methods: Ten patients were selected for a split-model study. In each patient, bone healing in SCPC-grafted and control ungrafted sockets was analyzed through clinical, radiographic, histomorphometric, and immunohistochemical assessments 6 months postoperatively. Results: A radiographic assessment using cone-beam computed tomography showed minimal ridge dimension changes in SCPC-grafted sockets, with 0.39 mm and 1.79 mm decreases in height and width, respectively. Core bone biopsy samples were obtained 6 months post-extraction during implant placement and analyzed. The average percent areas occupied by mature bone, woven bone, and remnant particles in the SCPC-grafted sockets were 41.3%±12%, 20.1%±9.5%, and 5.3%±4.4%, respectively. The percent areas of mature bone and woven bone formed in the control ungrafted sockets at the same time point were 31%±14% and 24.1%±9.4%, respectively. Histochemical and immunohistochemical analyses showed dense mineralized bundles of type I collagen with high osteopontin expression intensity in the grafted sockets. The newly formed bone was well vascularized, with numerous active osteoblasts, Haversian systems, and osteocytes indicating maturation. In contrast, the new bone in the control ungrafted sockets was immature, rich in type III collagen, and had a low osteocyte density. Conclusions: The resorption of SCPC granules in 6 months was coordinated with better new bone formation than was observed in untreated sockets. SCPC is a resorbable bone graft material that enhances bone formation and maturation through its stimulatory effect on bone cell function.

• Clinical and Experimental Reproductive Medicine
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• 제42권2호
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• pp.45-50
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• 2015

Objective: Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human in vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce calcium oscillation in oocytes and initiate the fertilization process. We evaluated the usefulness of AOA with a calcium ionophore in cases of total fertilization failure in previous cycles and in cases of severe male factor infertility patients with non-motile spermatozoa after pentoxifylline (PF) treatment. Methods: The present study describes 29 intracytoplasmic sperm injection (ICSI)-AOA cycles involving male factor infertility at Cheil General Hospital from January 2006 to June 2013. Patients were divided into two groups (control, n=480; AOA, n=29) depending on whether or not AOA using a calcium ionophore (A23187) was performed after testicular sperm extraction-ICSI (TESE-ICSI). The AOA group was further split into subgroups according to sperm motility after PF treatment: i.e., motile sperm-injected (n=12) and non-motile sperm-injected (n=17) groups (total n=29 cycles). Results: The good embryo rate (52.3% vs. 66.9%), pregnancy rate (20.7% vs. 52.1%), and delivery rate (10.3% vs. 40.8%) were lower in the PF/AOA group than in the control group. When evaluating the effects of restoration of sperm motility after PF treatment on clinical outcomes there was no difference in fertilization rate (66.6% vs. 64.7% in non-motile and motile sperm, respectively), pregnancy rate (17.6% vs. 33.3%), or delivery rate (5.9% vs. 16.7%) between the two groups. Conclusion: We suggest that oocyte activation is a useful method to ensure fertilization in TESE-ICSI cycles regardless of restoration of sperm motility after PF treatment. AOA may be useful in selected patients who have a low fertilization rate or total fertilization failure.

• Journal of Periodontal and Implant Science
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• 제31권3호
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• pp.555-564
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• 2001

The purpose of this study is to evaluate the bioresorbability of Calcium Polyphosphate added with $Na_2O$ and chitosan. Though calcium phosphate ceramics meet some of the needs for bone replacement, they have some limitation of unresorbability and fibrous encapsulation without direct bone apposition during bone remodelling. To solve these problem, we developed a new ceramic, calcium polyphosphate(CPP), and report the biologic response to CPP in extraction sites of beagle dog. Porous CPP granules were prepared by condensation of anhydrous $Ca(H_2PO_4)_2$ to form non-crystalline $Ca(PO_3)_2$. CPP granules added with $Na_2O$ and chitosan were implanted in extraction sockets and histologic observation were performed at 12 weeks later. Histologic observation at 12 weeks revealed that CPP matrix were mingled with and directly apposed to new bone without any intervention of fibrous connective tissue. CPP granules added with chitosan were well adatped without any adverse tissue reaction and resorbed slowly and spontaneously. CPP granules added with $Na_2O$ and chitosan show multinucleated giant cells and osteoblast-like cells around grafted material and newly formed bone. This result revealed that CPP, regardless of its additive component, had a high affinity for bone and had been resorbed slowly. From this results, it was suggested that CPP is promising ceramic as a bone substitute and addition of $Na_2O$ and chitosan help biodegradation. In further study , it will be determined which concentration of $Na_2O$ help biodegradation and the other additive components increase the degradation rate.