• 한국환경보건학회지
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• 제12권1호
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• pp.47-54
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• 1986

The purpose of the study is to determine the effects of sodium alginate on the suppression of organ accumulation of heavy metals were tested by mice. The seventy mice were divided into the control group and the experimental groups. The mice of cadmium group were subdivided into three groups by dose of 10 ppm cadmium group, adding 1% sodium alginate to the diets contaminated with 10 ppm cadmium group and adding 10% sodium alginate to the diets contaminated with 10 ppm cadmium group. The mice of copper group were subdivided into three groups by dose of 10 ppm copper group, adding 1% sodium alginate to the diets contaminated with 10 ppm copper group, and adding 10% sodium alginate to the diets contaminated with 10 ppm copper group. After the series of feeding of twenty-one days, the mice were killed and examined. Organs and feces were removed and analyzed for cadmium and copper amounts. The results obtained were as follows 1. As for average body weight gains, those of control group mice were the highest than heavy metal group and those of adding 10% sodium alginate to the diets contaminated with 10 ppm copper group the lowest. 2. The amount of cadmium accumulated in liver and kidney was higher than blood. The amount of cadmium in organs was higher in cadmium group than adding sodium alginate to the diets contaminated with cadmium group. 3. The amount of copper in liver was the highest, and that of copper in blood was the lowest. 4. The excretion of heavy metals was promotioned by adding 10% sodium alginate to the diets contaminated with 10 ppm heavy metal. ( P < 0.05 ).

• Applied Microscopy
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• 제32권4호
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• pp.361-376
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• 2002

본 연구는 카드뮴으로 유발된 생쥐 간중독에 대한 키토산올리고당의 효과를 알아보기 위하여 시도되었다. Mouse를 대상으로 카드뮴 (5.0 mg/kg; i.p.) 단독투여군(group Cd), 카드뮴과 키토산올리고당 (0.5% solution) 동시투여군 (group Cd+Chi)으로 구분한 후 간손상 억제효과를 알아보기 위해 간조직 중의 카드뮴 농도와 metallothionein 농도를 비교측정하였다. 또한 간조직의 형태학적 손상을 확인하기 위해 조직학적 관찰을 실시하였다. 간조직의 카드뮴 농도는 Cd군에 비해 Cd+Chi군에서 현저하게 낮게 나타났다. 간조직의 MT 농도는 Cd군이 Cd+Chi군에 비해서 낮게 나타났다. 전자현미경적 관찰 결과, Cd군에서 미토콘드리아는 심한 팽윤현상이 나타났으며, RER의 분절과 리보조옴 탈락이 관찰되었다. 그러나 Cd+Chi군에서는 전자밀도가 높은 다양한 형태의 미토콘드리아가 분포되어 있었으며, 리보조옴이 부착된 채로 전형적인 층판구조를 형성한 RER이 관찰되었다. 이상과 같은 결과로, 키토산올리고당이 생쥐 간조직에 미치는 카드뮴의 독성을 감소시킴을 알 수 있었다.

• Journal of Nutrition and Health
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• 제34권8호
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• pp.881-886
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• 2001

The purpose of this study was to Investigate the effects of green tea catechin on changes of mineral contents in chronic cadmium-poisoned rats. Sprague-Dawley male rats weighing 100 $\pm$ 10g were randomly assigned one of normal group and three cadmium poisoned groups. Cadmium groups were classified to catechin free diet(Cd-0C group), 0.25% catechin diet(Cd-0.25C group) and 0.5% catechin diet(Cd-0.5C group) according to the levels of catechin supplement. Animals were raised for 20 weeks. Cadmium was supplied in drinking water which contained 50ppm Cd$^{2+}$. Effects of catechin were analyzed on changes of mineral contents in chronic cadmium poisoned rats by determining the calcium accumulation in bones, blood, urine and faces and phosphorus In blood and urine. Cd-poisoning inducted the decrease of red blood cell(RBC), white blood cell(WBC), contents of blood hemoglobin and hematocrit, but the levels of those indices were increased by catechin supplementation. The contents of tibia and femur in Cd-0C group was significantly lower than in normal group, but those of catechin supplemetation group was similar to normal group. The calcium contents of urine and faces were higher in Cd-poisoned groups than in normal group, but they was lowered by catechin supplementation. The phosphorus contents of blood and urine in Cd-0C group was significantly lower than in normal group, but that of catechin supplementation group was similar to normal group. Catechin supplementation improved the calcium metabolism in chronic cadmium poisoned rats by increasing the contents of minerals such as calcium and phosphorus in blood and femur and by lowering the urinary and fecal calcium.m.

• Journal of Nutrition and Health
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• 제33권1호
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• pp.33-41
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• 2000

The purpose of this study was to investigate the effects of vitamin E on antioxidative defense system of liver in acute cadmium poisoned rats. Sprague-Dawley male rats weighing 100$\pm$10gm were randomly assigned to one control and three cadmium injected groups. Cadmium injected groups were fed vitamin E free diet(OE-Cd group), 40mg vitamin E per kg diet(40E-Cd group) or 400 mg vitamin E per kg diet(400E-Cd group). Vitamin E level of normal group was 40mg per kg diet. Animals were injected intraperitoneally with 2.0mg Cd$^2$$\^$+//kg bw for 4 days after the rats were fed diets with three different levels of vitamin E for 2 and 4weeks. Activities of superoxide dismutase(SOD), glutathione peroxidase(GSHPx) and glutathione S-transferase(GST) were decreased in cadmium injected groups but those were significantly improved by dietary vitamin I supplementations. Vitamin E contents reduced glutathione(GSH) in the live were decreased in cadmium injected groups, but we., not significantly different among three groups with different levels of vitamin E supplementations. Contents of liver thiobarbituric acid reactive substance (TBARS) of 0E-Cd group were higher than those of 400E-Cd and 400E-Cd groups, but those were markedly alleviated according to vitamin E supplementations. These results indicate that cadmium poisoning in rats causes decreasing antioxidative defense system and increasing peroxidative damage in liver, however can be restored by vitamin E supplements. (Korean J Nutrition 33 (1) : 33-41, 2000)

• 한국임상수의학회지
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• 제18권2호
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• pp.116-123
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• 2001

This study was undertaken to find out the effect of Korean safflower seed powder on histopathological changes of cadmium toxicity in mice. Fifty BALB/c mice were divided into a control group(A) and four experimental groups(B, C, D, E) : group A received tap water and basal diet, group B received tap water and diet supplemented with 3% Korean safflower seed powder alone, group C received basal diet and 300 $\mu\textrm{g}$/g of cadmium, group D and E received basal diet supplemented with 3% and 10% Korean safflower seed powder and 300$\mu\textrm{g}$/g of cadmium respectively. Cadmium dissolved in tap water was used, and the Korean safflower seed powder were mixed with feed. All mice were dissected on the 56th day. Histopathological changes in liver, kidney, lung, cortical osseous tissue of femoral shaft, bone trabecular of femur, and epiphyseal cartilage plate of femur were observed. Group B showed no significant changes compared with the control group. But group C showed the unclearness of specific cells in liver, the loss of architecture and focal necrosis of hepatocyte, the glomerular swelling, degeneration and necrosis of convoluted tubules, desquamation and vacuolization of the greater part of the renal tubular epithelium, the marked congestion and thickness of the wall of alveolus in lung, slightly thinning of the cortical osseous tissue in femoral shaft, reduction of cancellous bone volume and marked narrowness of bone trabecular, marked thinning of epiphyseal cartilage plate and irregular arrangement of columnar structure of cartilage cells. On the other hand, Korean safflower seed powder-treated group showed a little convalescent changes and maintained their normal architectures in liner, kidney, lung, cortical osseous tissue of femoral shaft, bone trabecular of femur and epiphyseal cartilage plate of femur.

• Applied Biological Chemistry
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• 제42권4호
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• pp.361-365
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• 1999

감나무 잎으로부터 분리한 폴리페놀 화합물을 가지고 카드뮴으로 중독 된 동물장기의 glutamic oxaloacetic transaminase(GOT), glutamic pyruvic transaminase(GPT), superoxide dismutase(SOD), glutathione S-transferase(GST) 활성 및 과산화지질에 미치는 영향을 조사하였다. GOT는 대조군에 대하여 P-1군(10,000 ppm)과 P-2군(25,000 ppm) 모두 유의적인 차이는 나타나지 않았으나 조금 낮았고, GPT 활성은 P-1군은 5% 수준에서, P-2군은 1% 수준에서 유의적으로 감소하였다. 간 조직의 SOD 활성을 측정한 결과, 대조군에 대하여 P-1군은 5% 수준에서 그리고 P-2군은 1% 수준에서 유의적으로 증가하였고, GST 활성은 대조군에 대하여 P-1군은 유의적인 차이가 나타나지 않았으나 조금 높았고, P-2군은 5% 수준에서 유의적으로 증가하였다. 과산화지질은 대조군에 대하여 P-1군과 P-2군 모두 1% 수준에서 유의적으로 증가하였다.

• Journal of Preventive Medicine and Public Health
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• 제24권3호
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• pp.287-304
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• 1991

Tolerance to several toxic effects of cadmium, including lethality has been shown following pretreatment with cadmium and zinc. This study was designed to determine if tolerance also develops to Cd-induced hepatotoxicityandrenaltoxicity. Three groups of rats (A, B, C), each consisting of 16 rats, were studied and each group was divided into four subgroups (1, 2, 3, 4), 4 rats for each subgroup. Rats were subcutaneously pretreated with saline (A), $CdCl_2$ (0.5 mg/kg, B), and $ZnCl_2$ (13.0 mg/kg, C) during time periods of $1{\sim}6$ weeks. At the end of the period, rats were challenged with $CdCl_2$ (3.0, 6.0 and 9.0 mg/kg, ip). After giving the challenge dose, cadmium and metallothionein (MT) concentrations were determined and also observed the histologic change in liver and kidney. The concentration of cadmium in liver and kidney increased dose-dependently to the challenge dosage. These da indicate the kidney is a major target organ of chronic cadmium poisoning, and suggest that cadmium induced hepatic injury, via release of Cd-MT, may play an important role in the nephrotoxicity observed in response to long-term exposure to cadmium. In addition, histologic examination of group $A_2,\;A_3\;and\;A_4$ revealed moderate to severe cadmium toxicity, evidenced by infiltration of inflammatory cells, cell swelling, pyknosis, enlarged sinusoids and necrosis in liver, and tubule cell necrosis and degeneration in kidney. However, MT concentrations in liver and kidney were increased by the pretreatment of $CdCl_2$ and $ZnCl_2$, and their morphological findings were not significantly changed, comparing with control group. Higher MT concentration in liver and kidney observed in the pretreated groups constitutes a plausible explanation of the protective effects of pretreatment against the cadmium toxicity after challenge dosing.

• 한국환경과학회지
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• 제7권1호
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• pp.46-51
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• 1998

The effects of aloe on the MDA(malondialdehyde) and the blood biochemical components of heavy metal poisoning in SD rat were examined and the following results were obtained. In rat liver homogenate intoxicated with $CdCl_2$, lipidperoxide was increased each 2.37times(24h), 3.31times(72h) but lipidperoxide In aloe administration groups was lower each 47% , 64% than in heavy metal group. In rat kidney homo- genate intoxicated with $CdCl_2$, lipidperoxide was increased 1.85times(24h), 1.33times(72h) but lipidperoxide in groups was almost the same as that of normal group. Lipidperoxide of kidney homogenate was slightly decreased as time passed. Also heavy metal poisoning rats showed high levels(1.38-2.50times) of serum AST, ALT and BUN. However. the administration of aloe significantly inhibited the reduction of them. These results suggest that Cd-induced hepatic and renal injury, via increase llpidpero)Ode and release of AST, ALT and BUN. Aloe may be used to inhibit or prevent the hepatic and renal toxicity which results from the heavy metal.

• 한국환경보건학회지
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• 제20권3호
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• pp.31-38
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• 1994

Dithiocarbamates containing polar groups which give very water soluble metal complexes were prepared from the reaction of carbon disulfide with diamines. The compounds have been characterized by elemental analysis, molar conductivity, and spectroscopic results. Dithiocarbamate and its complex were soluble in water. N, N-Dimethylammoniumpropylenedithiocarbamate(A) is clearly effective as inhibition of cis-platinum nephrotoxicity in rats. From the result of A rescue after cis-dichlorodiammineplatinum(II) treatment, it is suggested that dithiocarbamate removes platinum(II) coordinated to -SH groups bound to protein of kidney tubule cells by the reaction of platinum(II) with dithiocarbamate injected. A is effective as antidots for acute cadmium poisoning in Bacillus subtills. Growth of Bacillus subtills may be accelerated by A ligand dissociated from cadmium (II)-A complex.

• Nutritional Sciences
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• 제6권4호
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• pp.223-228
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• 2003

This study was carried out to investigate the effects of vitamin E on the cadmium contents of bone and on the calcium and phosphorous contents of the blood, urine and feces. Male Sprague-Dawley rats weighing 100$\pm$10g were randomly assigned to one normal group and three cadmium poisoned groups. The cadmium poisoned groups consisted of a vitamin E free diet (Cd-0E) group; a 40 mg vitamin E /kg diet (Cd-40E) group; and a 400 mg/kg diet (Cd-400E) group. Experimental animals were maintained on their respective diets for 20 weeks and were simultaneously administered 50 ppm $Cd^{2+}$ dissolved in the drinking water. At the end of the trial, the average hematocrit value in the Cd-0E group was 28.13% lower than in the normal group. However, the average hematocrit value in the Cd-400E group was significantly higher than in the Cd-0E and Cd-40E groups. WBC levels in the cadmium-poisoned groups were lower than in the normal group, but Cd-400E group levels were significantly higher than in the Cd-0E and Cd-40E groups. The contents of calcium of tibia has no significant difference between normal group and cadmium exposed group at $10^{th}$ week After 20 weeks, the calcium contents of the tibia in the Cd-0E and Cd-40E groups were lower than in the normal group by 25.5% and 22.1 %, respectively, although the calcium contents of the tibia in the Cd-400E group were higher than in the normal group. After 10 weeks, the calcium contents of the femur in the Cd-0E and Cd-40E groups were 19.25% and 15.45% lower than in the normal group, respectively, but the calcium contents of the femur in the Cd-400E group were at the same levels as in the normal group. The levels of calcium in the femur after 20 weeks were similar to the 10-week levels. Calcium levels of the urine in the Cd-0E and Cd-40E groups were 3.92 fold and 2.92 fold higher, respectively, than in the normal group, but levels in the Cd-400E group were significantly lower than in either the Cd-0E group or the Cd-40E group. Calcium levels of the feces in cadmium-poisoned groups were significantly higher than in the normal group, although levels in the Cd-400E group were significantly lower than in the Cd-0E and Cd-40E groups. Phosphorous levels of the blood in the Cd-0E group were 17% lower than in the normal group, although levels in the Cd-400E group were significantly higher than in the Cd-0E group. Phosphorous levels of the urine in the Cd-0E and Cd-40E groups were significantly higher than in the normal group, while Cd-400E group levels were found to be at the same level as in the normal group. Cadmium contents of the tibia in the Cd-40E and Cd-400E groups were 13% and 17% lower, respectively, than in the Cd-0E group. Regarding cadmium levels in the femur, only the Cd-400E group achieved lower levels (10% lower) than the Cd-0E group. In conclusion, vitamin E supplementation resulted in a suppression of the release of calcium from bone, and a reduction in the excretion of calcium via the urine and feces, thus having a normalizing effect on calcium metabolism in rats with chronic cadmium poisoning.