• Journal of Crop Science and Biotechnology
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• 제10권1호
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• pp.19-26
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• 2007

Glutathione S-transferases(GSTs, EC 2.5.1.18), glyoxalase-I(EC 4.4.1.5) and alliin lyase(alliinase, EC 4.4.1.4) are important enzyme systems in plant bodies. The first two are mainly detoxifying enzymes that utilize glutathione(GSH) in the defense mechanism, and the last one is mainly involved in secondary metabolism and relevant to sulfur compounds derived from GSH. The activities of the three enzymes have been investigated in soluble extracts of vegetable crops, including pumpkin, cabbage, broccoli, radish, carrot, potato, sweet potato, mungbean, and onion. GST activities were detected in all of the vegetables, and the extract of onion bulb exhibited the highest specific activity(648 nmol/min/mgP). The putative GSTs of most of the vegetables were found to be induced by ethanol. The activities of GSTs in onion bulb were found to be markedly inhibited by S-hexyl glutathione and were also inhibited by S-butyl glutathione and S-propyl glutathione. The anti-CmGSTF1 antiserum recognized a thick band for putative onion GST. The estimated glyoxalase-I activity level was also high in onion bulb(4540 nmol/min/mgP), indicating that the thick band detected by Western blot analysis might result from partial recognition of glyoxalase-I by the antiserum. The specific activities for glyoxalase-I were moderate in radish and carrot, and the extracts of other vegetables had rather low levels of activities. The extract of onion also showed the highest specific activity level for alliinase(2069nmol pyruvate/mgP). The extracts of other vegetables also had alliinase activities, although the estimated values were much lower than that of onion.

• 한국산업보건학회지
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• 제33권2호
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• pp.230-246
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• 2023

Objectives: The climate crisis has arrived and heat-related illnesses are increasing. It is necessary to discover new high-heat risk industries and understand the environment . It is also necessary to prioritize risks of industries that have not been included in the management target to date. The study was intended to monitor and evaluate the thermal risk of high-priority workplaces. Methods: A prioritization method was developed based on five factors: occurrence of and death due to heat-related illnesses, work environment monitoring, indoor work rate, small heat source, and limited heat dissipation. it, was applied to industrial accidents caused by heat-related illnesses. Wet bulb temperature index and apparent temperature were measured in July and August at 24 workplaces in seven industries and assessed for thermal risk. Results: The wet bulb temperature index was in the range of 23.8~31.9℃, and exposure limits were exceeded in the growing of crops, food services activities and accommodation, and building construction. The apparent temperature was in the range of 26.8~36.7℃, and exceeded the temperature standard for issuing heatwave warnings in growing of crops, food services activities and accommodation, warehousing, welding, and building construction. Both temperature index in growing of crops and building construction were higher than the outside air temperature. Conclusions: In the workplace, risks in industries that have not be controlled and recognized through existing systems was identified. it is necessary to provide break times according to the work-rest time ratio required during dangerous time period.

• 한국식품저장유통학회지
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• 제8권3호
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• pp.258-263
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• 2001

마늘을 $0^{\circ}C$에서 일반 대기 및 CA 조건 하에서 4개월 저장하면서 기간 및 조건에 따른 저장 중 마늘의 생리적 품질을 폭정하고 pungency와 fructan 등의 화학적 성분 변화를 분석하였다. 저장 중 마늘의 생리적 품질은 저장 조건에 대해서 큰 차이는 없었으나, CA 저장한 마늘이 일반 대기에서 저장한 마늘에 비해서 수분 감량이 더 많이 일어나 dry weight와 경도가 낮게 나타났다. 표면색은 저장 4개월에서 어둡고 진하게 변했다. CA 저장이 마늘의 발근은 억제하지 못하지만 발아율은 일반 대기저장에 비해서 약 50% 억제하는 것으로 나타났다. 일반 대기에서 저장한 마늘이 CA 저장한 마늘보다 더 높은 pungency를 나타내었으며 특히 pyruvate는 산소 농도가 닻은 (1% $O_2$) CA 저장 조건에서 가장 낮은 값을 나타내었다. Fructan은 저장기간에 따라서 점차 감소하는 경향을 나타내었고 fine sugar는 점차 증가하였다. 발아율이 높은 일반 대기 저장의 fructan 함량이 CA 저장의 경우보다 낮은 값을 나타내었다 그러나 20%의 이산화탄소 농도에서 저장한 마늘의 fructan이 가상 많이 감소하는 경향을 보였다

• 생물환경조절학회지
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• 제28권2호
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• pp.104-109
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• 2019

식물공장에 재배 가능한 작물은 매우 다양할 것으로 본다. 식물공장에서 재식밀도에 대한 중요성이 인식되고 있다. 본 연구는 완전제어형 식물공장에서 콜라비의 재배에 적합한 재식밀도를 구명하기 위하여 수행되었다. 식물공장 형태는 형광등을 이용한 완전제어형태로, 박막수경재배를 이용하여 재배하였다. 재식밀도는 $22plants/m^2(15{\times}30cm)$, $27plants/m^2(15{\times}25cm)$, 그리고 $33plants/m^2(15{\times}20cm)$로 처리하였다. 식물체당 지상부 생체중과 건물중 또는 식물체당 벌브의 지상부 생체중와 건물중에는 재식밀도간 유의적인 차이를 보이지 않았다. 단위면적당 지상부 생체중과 건물중 또는 단위면적당 벌브의 지상부 생체중과 건물중에는 재식밀도가 높은 처리구($33plants/m^2$)에서 높게 나타났다. 재식밀도와 초장, 엽면적, 광합성, 경도 및 엽록소간에는 유의적인 차이를 보이지 않았다. 재식밀도와 벌브 높이와 지름 및 당도간에는 유의적인 차이를 보였다. 재식밀도가 낮은 $22plants/m^2$ 처리구에서 가장 높은 벌브 높이와 지름을 보였으며, 당도 또한 높았다. 이상의 결과를 바탕으로 결론을 내리면, 경제성을 고려한 생육적인 측면에서는 단위면적당 생산량이 많은 재식밀도 $33plants/m^2(15{\times}20cm)$가 적정하였으나, 당도와 같은 품질적인 측면에서는 재식밀도 $22plants/m^2(15{\times}30cm)$가 적정하였다.

• 농업과학연구
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• 제49권1호
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• pp.113-120
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• 2022

Leaf blight disease caused by Stemphylium species is an important disease in Allium crops, specifically onion, garlic and welsh onion. In 2018, leaf blight symptoms were severe and damaged onion and garlic in Jeonnam province in Korea. In addition, small purple spots on garlic burbs were observed in a post-harvest storage warehouse. Several Stemphylium isolates were isolated from diseased leaves from the field and from garlic bulb samples and were analyzed in terms of homology and the phylogenetic relationship based on the internal transcribed spacer region and calmodulin gene sequence. The results showed that among three Stemphylium species identified, S. vesicarium is most prevalent on onion and garlic. S. eturmiunum was for the first time identified as pathogenic to onion and garlic, whereas S. solani was found in welsh onion crops. Although these isolates grew well at the optimum temperature at 20 - 25℃, they could also grow at low temperatures of 10 - 15℃. A pathogenicity test was conducted using S. vesicarium and S. eturmiunum on onion and garlic respectively. These results showed that two Stemphylium species were highly virulent with cross pathogenicity in onion and garlic. The results of this study can support the biological characterization of Stemphylium species in Korea. Moreover, further research will need to develop fungicide application strategies for onion and garlic crops.

• Journal of Plant Biotechnology
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• 제6권1호
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• pp.9-13
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• 2004

Transient uidA expression was used to optimize parameters required for biolistic transformation of suspension cells of Easter lily, Lilium longiflourm. Maximum uidA expression occurred following bombardment with gold particles as compared to tungsten. A 3hr pre-treatment of suspension cells with 0.125M osmoticum resulted in a 1.5X increase in uidA expression. A helium pressure of 1550 psi combined with a particle travelling distance of 6cm resulted in maximum uidA expression as compared to either 1100, 1200, or 1800 psi. Transient transformation resulted in up to 493 uidA expressing cells/Petri plate. For stable transformation suspension cells of Lilium longiflorum, were co-bombarded with plasmid DNA containing cucumber mosaic virus (CMV) replicase under the rice actin (Act1) promoter and either the bar or PAT genes under the cauliflower mosaic virus (CaMV 355) promoter. Ten regenerated plants contained the transgene as analyzed by PCR, and two of the ten plants were confirmed to contain the transgene by Southern hybridization. The two transgenic plants were independent transformants, one containing the bar gene and the other both the CMV replicase and bar genes. Plants were sprayed at the rosette stage and found to be resistant to 1000 mg/L of phosphinothricin (Trade name-Ignite) indicating expression of the bar gene throughout the leaves when bar was under control of the CaMV 35S promoter.

• 한국농공학회지
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• 제16권2호
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• pp.3430-3437
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• 1974

.It is very importaut to know the water consumption of crops in planning irrigation works and practicing suitable soil moisture management. For the purpose of making it clear that how much water be consumed to cultivate the Chinese cabbage, Chamber method has been applied. Main equipments in the transpiration chamber are flowers, manometer and electric thermograph. The chamber made of vynyl plate has a small entrance at the base and an exit at the top, and the ventilation in the chamber was carried out by a flower through the entrance and exit. Air-flow adjusted by an orifice manometer enters the chamber from the outside over the crop canopy through the pipe like a chimney and finally goes out to the outside. Two sets which consist of a pair of dry and wet bulb made by thermistor are installed in the entrance and exit tube, and record air temperature automatically. Evapotranspiration amount is computed from the air-flow quantity and difference in absolute humidity between at the entrance and exit of the chamber by the following equation: ET=(X2-X1)${\times}$Q where ET=evapotranspiration amount X1=absolute humidity at the entrance(g/㎥) X2=absolute humidity at the exit(g/㎥) Q=air-flow quantity(㎥) This study was carried out at the upland farm of the Institute of Agriculture Engimeering and Utilization, Suwon, Korea. from 1971 to 1973. The results obtained in this experiment are as follows: 1. The total amount of evapotranspiration of Chinese Cabbage that is cultivated in autumn is 408.1mm during growth period. 2. Chinese cabbage rapidly grows up in the second ten days of September, 40th to 50th days after seeding. At the same time, the maximum amount of evaportranspiration of Chinese cabbage is 61.6mm/10 days 3. The correlation between Pan-evaporation and evapotranspiration is high, coefficient of correlation r=0.88**, and can be shown as The following regression equation: ET=0.913E+20.273 4. Evapotranspiration is closely related with meteorological factors: r=0.85**, for insolation, r=0.76** for air temperature, respectively. 5. The percentage of evapotranspiration amount, at the beginning of growth stage, gradually increases in proportion as the Chinese Cabbage grows but is largely affected by meteorological factors after the green cover formation. 6. By Blaney and Griddle formula, evaportranspiration coefficient "K" are within from 0,85 to 1.27.

• 한국작물학회지
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• 제65권1호
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• pp.72-78
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• 2020

본 연구는 탄질비(C/N)가 224인 억새 절삭물을 토양에 시용하여 토양을 개선하고 작물 생육을 촉진하고자 ha 당 무시용, 10톤, 20톤을 처리하였다. 그 결과, 토양 유기물 함량이 시험 전 11.0 g·kg^-1에서 3년 후 최대 16.3까지 증가하였다. 토양 양이온치환용량의 경우 3년 후 최대 15.3 cmol_c·kg^-1까지 증가하였다. 앞그루작물인 단수수는 20톤 시용구가 줄기가 굵었고 주당 착즙량이 60 ml로 가장 많았다. 착즙액량과 착즙액의 가용성고형물 함량을 곱한 수량지수는 억새 10톤 시용구가 1,913으로 가장 높고 20톤 시용구와 대조구가 각각 1,851, 1,839 순이었다. 억새 바이오매스 시용량이 늘어나고 시험 연차가 길어짐에 따라 일시적 비절과 가뭄 피해가 발생하였다. 고구마 지상부 생육은 억새 시용의 영향을 받지 않았다. 상품(marcketable) 괴근 수는 억새 20톤 시용구가 대조구와 같은 2,9개였고, 10톤 시용구가 3.6개로 가장 많았다. 억새 20톤 시용구와 대조구의 2년간 평균수량이 각각 2,579 kg/10a, 2,708으로 낮았고 10톤 시용구가 3,289로 가장 많았다. 뒷그루작물인 양파의 경우 억새 시용에 따른 수량 차이는 인정되지 않았으나 억새 20톤 시용구가 무시용구나 10톤 시용구에 비해 양파 초장과 엽장이 길었다. 마늘의 수량은 2,630~2,901 kg/10a으로서 억새 시용의 영향이 없었고, 억새 10톤 시용구가 초장과 엽장이 가장 길었고, 인편 수는 억새 시용구가 인경구당 8.2~8.3개였고 대조구가 8.9개로 가장 많았다. 본 시험 결과, ha 당 10톤 내외의 억새 바이오매스를 시용할 경우 토양 개량과 더불어 작물 생육과 수량을 촉진시켜 향후 부존자원인 억새의 가치를 재발견하는 실마리가 될 것으로 보인다.

• 원예과학기술지
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• 제33권6호
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• pp.829-838
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• 2015

본 연구는 마늘의 초기생장에 미치는 온도의 영향을 살펴보기 위하여 맹아가 출현된 마늘의 인편을 온도를 달리하여 재배하고, 마늘의 생육특성, 광계II 활성과 항산화효소의 발현 양상을 살펴보았다. 마늘의 생육은 $15-25^{\circ}C$ 조건에서 양호하여 지상부의 생장과 엽수가 빠르게 발달하였다. 특히, 지상부와 인경의 건중량과 전체 건중량이 $20^{\circ}C$에서 가장 높았으며, 그보다 높은 온도에서는 지상부, 인경과 뿌리의 건중량이, 그리고 낮은 온도에서는 지상부의 건중량이 크게 감소하였다. $F_v/F_o$와 $F_v/F_m$ 또한 $15-20^{\circ}C$에서 높았으며 그 이상의 온도에서 크게 감소하였다. OKJIP 곡선의 패턴에서도 $15-20^{\circ}C$에서 $F_i$, $F_p$가 다소 증가하고, $25^{\circ}C$ 이상의 온도에서는 점차 감소하였으며, $F_k$와 $W_k$는 $30^{\circ}C$에서 뚜렷하게 증가하였다. 그리고, 항산화효소들 중에 잎의 catalase와 superoxide dismutase, 그리고 뿌리의 peroxidase는 $20-25^{\circ}C$에서 높고 $30^{\circ}C$에서 크게 낮았다. 이러한 결과는 $30^{\circ}C$ 조건이 마늘의 초기생장에 있어 스트레스 요인으로 작용하고 있으며, $20^{\circ}C$ 전후에서 재배하는 것이 바람직함을 나타내 주고 있다. 그리고, $F_v/F_o$, $F_v/F_m$, $F_k$, $ET_o/CS_m$, $PI_{abs}$ 등의 엽록소형광 변수들은 마늘의 전체 건중량과 의미있는 상관을 보여, 마늘 생산성을 사전에 예측하는데 유용한 변수로 활용될 수 있을 것으로 보인다.