• Journal of Crop Science and Biotechnology
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• v.10 no.1
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• pp.19-26
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• 2007

Glutathione S-transferases(GSTs, EC 2.5.1.18), glyoxalase-I(EC 4.4.1.5) and alliin lyase(alliinase, EC 4.4.1.4) are important enzyme systems in plant bodies. The first two are mainly detoxifying enzymes that utilize glutathione(GSH) in the defense mechanism, and the last one is mainly involved in secondary metabolism and relevant to sulfur compounds derived from GSH. The activities of the three enzymes have been investigated in soluble extracts of vegetable crops, including pumpkin, cabbage, broccoli, radish, carrot, potato, sweet potato, mungbean, and onion. GST activities were detected in all of the vegetables, and the extract of onion bulb exhibited the highest specific activity(648 nmol/min/mgP). The putative GSTs of most of the vegetables were found to be induced by ethanol. The activities of GSTs in onion bulb were found to be markedly inhibited by S-hexyl glutathione and were also inhibited by S-butyl glutathione and S-propyl glutathione. The anti-CmGSTF1 antiserum recognized a thick band for putative onion GST. The estimated glyoxalase-I activity level was also high in onion bulb(4540 nmol/min/mgP), indicating that the thick band detected by Western blot analysis might result from partial recognition of glyoxalase-I by the antiserum. The specific activities for glyoxalase-I were moderate in radish and carrot, and the extracts of other vegetables had rather low levels of activities. The extract of onion also showed the highest specific activity level for alliinase(2069nmol pyruvate/mgP). The extracts of other vegetables also had alliinase activities, although the estimated values were much lower than that of onion.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.33 no.2
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• pp.230-246
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• 2023

Objectives: The climate crisis has arrived and heat-related illnesses are increasing. It is necessary to discover new high-heat risk industries and understand the environment . It is also necessary to prioritize risks of industries that have not been included in the management target to date. The study was intended to monitor and evaluate the thermal risk of high-priority workplaces. Methods: A prioritization method was developed based on five factors: occurrence of and death due to heat-related illnesses, work environment monitoring, indoor work rate, small heat source, and limited heat dissipation. it, was applied to industrial accidents caused by heat-related illnesses. Wet bulb temperature index and apparent temperature were measured in July and August at 24 workplaces in seven industries and assessed for thermal risk. Results: The wet bulb temperature index was in the range of 23.8~31.9℃, and exposure limits were exceeded in the growing of crops, food services activities and accommodation, and building construction. The apparent temperature was in the range of 26.8~36.7℃, and exceeded the temperature standard for issuing heatwave warnings in growing of crops, food services activities and accommodation, warehousing, welding, and building construction. Both temperature index in growing of crops and building construction were higher than the outside air temperature. Conclusions: In the workplace, risks in industries that have not be controlled and recognized through existing systems was identified. it is necessary to provide break times according to the work-rest time ratio required during dangerous time period.

• Food Science and Preservation
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• v.8 no.3
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• pp.258-263
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• 2001

This experiment was conducted to measure the physiological qualities and the chemical compositions of garlic bulb (Allium sativum L) stored in air and controlled atmosphere at 0$\^{C}$ for 4months. There were no significant differences in physiological qualities such as soluble solid, dry weight and firmness by O$_2$ and CO$_2$ concentrations during 4 months of storage. The surface color of garlic cloves changed toward more dark direction in Hunter color surface with storage. CA storage inhibited the sprout growth by 50% and had no significant difference in root growth. Garlic stored in CA showed lower level of pungency, especially CA of low oxygen concentration (1%) showed the lowest level of pyruvate. Garlic stored in high CO$_2$ concentration(20%) showed the lowest level of fructan concentrations and the highest free sugar concentrations.

• Journal of Bio-Environment Control
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• v.28 no.2
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• pp.104-109
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• 2019

The crops recommended for the plant factory system are diverse. The importance of planting density in the plant factory is being recognized. The objective of this study was to determine the optimal planting density for growth and quality of kohlrabi in a closed-type plant factory system. The kohlrabi was grown under fluorescent lamps and nutrient film technique system. The growth and quality of kohlrabi were investigated under four different planting densities ($22plants/m^2(15{\times}30cm)$, $27plants/m^2(15{\times}25cm)$, and $33plants/m^2(15{\times}20cm)$). There were no significant interactions between Shoot fresh and dry weights per plant or bulb stem fresh and dry weights per plant and planting density. Shoot fresh and dry weight per area or bulb stem fresh and dry weight per area were the highest at $33plants/m^2$. There were no significant interactions between plant height, leaf area, photosynthetic rate, hardness, and chlorophyll content and planting density. Significant differences in Bulb stem height and diameter, and brix were observed. Bulb stem height and diameter and brix of kohlrabi were the highest at $22plants/m^2$. Based on our results, we conclude that the optimal planting density is $33plants/m^2$ for growth of kohlrabi, however, the optimal planting density is $22plants/m^2$ for quality of kohlrabi in a closed-type plant factory system.

• Korean Journal of Agricultural Science
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• v.49 no.1
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• pp.113-120
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• 2022

Leaf blight disease caused by Stemphylium species is an important disease in Allium crops, specifically onion, garlic and welsh onion. In 2018, leaf blight symptoms were severe and damaged onion and garlic in Jeonnam province in Korea. In addition, small purple spots on garlic burbs were observed in a post-harvest storage warehouse. Several Stemphylium isolates were isolated from diseased leaves from the field and from garlic bulb samples and were analyzed in terms of homology and the phylogenetic relationship based on the internal transcribed spacer region and calmodulin gene sequence. The results showed that among three Stemphylium species identified, S. vesicarium is most prevalent on onion and garlic. S. eturmiunum was for the first time identified as pathogenic to onion and garlic, whereas S. solani was found in welsh onion crops. Although these isolates grew well at the optimum temperature at 20 - 25℃, they could also grow at low temperatures of 10 - 15℃. A pathogenicity test was conducted using S. vesicarium and S. eturmiunum on onion and garlic respectively. These results showed that two Stemphylium species were highly virulent with cross pathogenicity in onion and garlic. The results of this study can support the biological characterization of Stemphylium species in Korea. Moreover, further research will need to develop fungicide application strategies for onion and garlic crops.

• Journal of Plant Biotechnology
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• v.6 no.1
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• pp.9-13
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• 2004

Transient uidA expression was used to optimize parameters required for biolistic transformation of suspension cells of Easter lily, Lilium longiflourm. Maximum uidA expression occurred following bombardment with gold particles as compared to tungsten. A 3hr pre-treatment of suspension cells with 0.125M osmoticum resulted in a 1.5X increase in uidA expression. A helium pressure of 1550 psi combined with a particle travelling distance of 6cm resulted in maximum uidA expression as compared to either 1100, 1200, or 1800 psi. Transient transformation resulted in up to 493 uidA expressing cells/Petri plate. For stable transformation suspension cells of Lilium longiflorum, were co-bombarded with plasmid DNA containing cucumber mosaic virus (CMV) replicase under the rice actin (Act1) promoter and either the bar or PAT genes under the cauliflower mosaic virus (CaMV 355) promoter. Ten regenerated plants contained the transgene as analyzed by PCR, and two of the ten plants were confirmed to contain the transgene by Southern hybridization. The two transgenic plants were independent transformants, one containing the bar gene and the other both the CMV replicase and bar genes. Plants were sprayed at the rosette stage and found to be resistant to 1000 mg/L of phosphinothricin (Trade name-Ignite) indicating expression of the bar gene throughout the leaves when bar was under control of the CaMV 35S promoter.

• Magazine of the Korean Society of Agricultural Engineers
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• v.16 no.2
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• pp.3430-3437
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• 1974

.It is very importaut to know the water consumption of crops in planning irrigation works and practicing suitable soil moisture management. For the purpose of making it clear that how much water be consumed to cultivate the Chinese cabbage, Chamber method has been applied. Main equipments in the transpiration chamber are flowers, manometer and electric thermograph. The chamber made of vynyl plate has a small entrance at the base and an exit at the top, and the ventilation in the chamber was carried out by a flower through the entrance and exit. Air-flow adjusted by an orifice manometer enters the chamber from the outside over the crop canopy through the pipe like a chimney and finally goes out to the outside. Two sets which consist of a pair of dry and wet bulb made by thermistor are installed in the entrance and exit tube, and record air temperature automatically. Evapotranspiration amount is computed from the air-flow quantity and difference in absolute humidity between at the entrance and exit of the chamber by the following equation: ET=(X2-X1)${\times}$Q where ET=evapotranspiration amount X1=absolute humidity at the entrance(g/㎥) X2=absolute humidity at the exit(g/㎥) Q=air-flow quantity(㎥) This study was carried out at the upland farm of the Institute of Agriculture Engimeering and Utilization, Suwon, Korea. from 1971 to 1973. The results obtained in this experiment are as follows: 1. The total amount of evapotranspiration of Chinese Cabbage that is cultivated in autumn is 408.1mm during growth period. 2. Chinese cabbage rapidly grows up in the second ten days of September, 40th to 50th days after seeding. At the same time, the maximum amount of evaportranspiration of Chinese cabbage is 61.6mm/10 days 3. The correlation between Pan-evaporation and evapotranspiration is high, coefficient of correlation r=0.88**, and can be shown as The following regression equation: ET=0.913E+20.273 4. Evapotranspiration is closely related with meteorological factors: r=0.85**, for insolation, r=0.76** for air temperature, respectively. 5. The percentage of evapotranspiration amount, at the beginning of growth stage, gradually increases in proportion as the Chinese Cabbage grows but is largely affected by meteorological factors after the green cover formation. 6. By Blaney and Griddle formula, evaportranspiration coefficient "K" are within from 0,85 to 1.27.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.1
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• pp.72-78
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• 2020

In this study, miscanthus with C/N ratio of 224 were applied to the soil and treated with 0 (control), 10 tons and 20 tons·ha^-1 to improve the soil and promote crop growth. As a result, soil organic matter content increased from 11.0 g·kg^-1 before the test to 16.3 after 3 years. Soil cation exchangeable capacity increased to 15.3 cmol_c·kg^-1 after 3 years. In the sweet sorghum, stem was the most thickest at 20 tons·ha^-1 application of miscanthus and the highest juice amount per plant was 60 ml. The yield index multiplied by the soluble solids content of juice and juice amount was the highest at 1,913 for 10 tons application and 1,851, 1,839 for 20 tons, control respectively. Number of sweetpotato storage root were 2,9 in 20-tons application plot, the same as control, and 10-tons application plot was 3.6, the most. Two-year average yields of 20 tons plot and control were low at 2,579 kg/10a and 2,708 respectively, and 10 tons plot was the highest at 3,289. For onions, the biomass application did not effect the yield. but onion plant and leaf length were longer in 20 tons plot than in control or 10 tons. The yield of garlic was 2,630~2,901 kg/10a and there was no effect of miscanthus application. Plot of 10 tons application were the longest in plant and leaf length, and the number of scale was 8.2-8.3 per in bulb, and 8.9 tons·ha^-1 in control. Therefore, it was confirmed the possibility that miscanthus biomass application of about 10 tons·ha^-1 could improve the soil condition and promote crops growth and yield.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.829-838
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• 2015

Garlic (Allium sativum L.), one of the oldest cultivated crops, is the most widely used Allium species belonging to the family Lilliaceae. In this study, growth characteristics, photosystem II activity, and antioxidative enzyme activity were investigated in five temperatures ($10-30^{\circ}C$) during early growth stage of garlic to determine the optimum temperature for cultivation and assess the effects of high temperature on early growth of garlic. Vegetative growth (e.g., shoot height, number of leaves) of garlic plants was greater in the temperature ranges of $15-25^{\circ}C$. However, dry weight (of shoot, bulb, and total plant) of garlic was significantly greater at $20^{\circ}C$, compared to either below or above $20^{\circ}C$. $F_v/F_o$ and $F_v/F_m$ values were highest at $15-20^{\circ}C$, and decreased above $25^{\circ}C$. The chlorophyll a fluorescence induction OKJIP transient was also considerably affected by high temperature; the fluorescence yields $F_i$ and $F_P$ decreased considerably above $25^{\circ}C$, with the increase of $F_k$ and $W_k$. Activities of catalase and superoxide dismutase in leaves and peroxidase in roots were high in $20-25^{\circ}C$, and decreased significantly in $30^{\circ}C$. These results indicate that a growth temperature of $30^{\circ}C$ inhibits early growth of garlic and that it is desirable to culture garlic plants near $20^{\circ}C$. Fluorescence parameters such a $F_v/F_o$, $F_v/F_m$, $F_k$, $ET_o/CS_m$, and $PI_{abs}$ were significantly correlated with dry weight of whole garlic plants (p < 0.01), indicating that these fluorescence parameters can be used for early assessment of high temperature effects even though the damage to the plant is not very severe.