• Korean Journal of Food Science and Technology
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• v.34 no.5
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• pp.787-791
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• 2002

Optimal extraction, separation, and capillary rinsing conditions for capillary electrophoresis (CE) were established to identify the cultivation site (domestic vs. foreign) of Job's-tears (Coix lachrymajobi var. mayuen) using 240 samples (domestic sample n = 121, foreign sample n = 119). Job's-tears was extracted with 30% ethanol and separated on a $50-{\mu}m-I.D.$ untreated fused-silica capillary. Optimal analytic conditions were: temperature, $45^{\circ}C$; voltage, 15 kV; detector rise time, 0.1 sec; and pressure injection, 20 sec. Separation of peak investigated using 0.1 M phosphate buffer (pH 2.5) containing 0.05% hydroxypropylmethylcellulose (P buffer) revealed the optimal separation buffer was P buffer containing 26 mM hexane sulfonic acid with 30% methanol. Under the optimal conditions established for CE, the average correct identification percentage of domestic or foreign Job's-tears was 82%.

• Korean Journal of Veterinary Research
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• v.33 no.4
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• pp.623-632
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• 1993

This study was conducted to develop a sensitized latex-antigen for serodiagnosis of toxoplasmosis in animals. Tachyzoites of T gondii(RH-strain) harvested from mouse peritoneal cavity were purified through the filtraton of polycarbonate membrane(pore size, $3.0{{\mu}m}$, Costar Co.) and disrupted by ultrasonicator. The tachyzoite suspension was ultracentrifuged for 30 min at $60,000{\times}g(4{^{\circ}C})$ and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of $0.8{{\mu}m}$ in diameter(Sigma) were used for the preparation of sensitized latex-antigen suspension. The several parameters including the preparation conditions, incubation buffer. serum dilution buffer and stability of agglutination reactions were evaluated and the results obtained were summarized as follows : 1. The antigen consisting of a water-lysate of T gondii tachyzoites was adsorbed onto polystyrene latex particles of $0.8{{\mu}m}$ in diameter by adding a latex suspension to an equal volume of diluted antigen solution and by incubating the mixture at $37{^{\circ}C}$ under different conditions. 2. The optimum incubation buffer used for the antigen sensitization was 0.1M Tris-HCl buffer(pH 8.0). 3. The optimum serum dilution buffer used for the latex agglutination test was 0.1M Tris-HCl-NaCl buffer(pH 7.4) containing 300 mM NaCl. But 0.1M Tris-HCl-NaCl buffer(pH 7.4) containing 300-600 mM NaCl, 0.5% BSA and 0.01% Tween-20 improved the agglutination pattems and cleared the background of microplate well without the effects on L.A titer. 4. The time required for antigen sensitization was 40 and 60 min in incubation buffer(pH 8.0) at $37{^{\circ}C}$. But the optimun time for antigen sensitization was min at $37{^{\circ}C}$. 5. The optimun quantity of antigen absorbed on latex particles for proper agglutination was the range of 20 to $32{\mu}g$ of latex particles. 6. The optimun concentration of the latex-antigen suspension for the proper agglutination reaction was determined as 0.2%(w/v). 7. The specificity, rapidity and simplicity of the latex-particle agglutination test suggested that it might be adaptable to large scale serum screening.

• Journal of the Korean Institute of Telematics and Electronics C
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• v.35C no.11
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• pp.39-47
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• 1998

This paper describes high-speed pipelined memory architecture for a shared buffer ATM switch. The memory architecture provides high speed and scalability. It eliminates the restriction of memory cycle time in a shared buffer ATM switch. It provides versatile performance in a shared buffer ATM switch using its scalability. It consists of a 2-D array configuration of small memory banks. Increasing the array configuration enlarges the entire memory capacity. Maximum cycle time of the designed pipelined memory is 4 ns with 5 V V$\_$dd/ and 25$^{\circ}C$. It is embedded in the prototype chip of a shared scalable buffer ATM switch with 4 x 4 configuration of 4160-bit SRAM memory banks. It is integrated in 0.6 $\mu\textrm{m}$ 2-metal 1-poly CMOS technology.

• Journal of Navigation and Port Research
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• v.45 no.6
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• pp.314-324
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• 2021

Competition between ports around the world is intensifying to attract transshipment cargo. However, recently, there have been concerns about the departure of transshipment cargo volume at Busan Port, such as the lifting of the cabotage policy in China's shipping sector and the implementation of a safe fare system. In terms of operation, terminal congestion and vehicle waiting time are seriously occurring due to imbalance in the transshipment volume of each terminal and vehicles concentrated in a specific time period. In this paper, we propose a method of inter-terminal transportation (ITT) using buffer space to solve the problem caused by inefficient ITT systems and presented a mixed integer programming (MIP) for the problem. The effect of using the buffer space was analyzed for various work volumes and capacity fluctuation ranges by applying the terminal congestion pattern and ITT vehicle in/out pattern based on the Busan New Port data.

• Journal of Environmental Impact Assessment
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• v.17 no.1
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• pp.1-9
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• 2008

Non-point pollution is caused by many diffusive sources, unlike a point pollution derived from industrial wastewater treatment plants or sewage treatment plants. Runoff of non-point pollutants is originated from rainfall or thawing in short period of time moving over and through the a ground surface. They cause ill effect on the quality of neighboring aquatic environment. To prevent effectively the wash off from non-point pollutant, it should be immediately reduced at the source or be treated after gathering of runoff water. This study has been carried out for the best width of riparian buffer zone. So we implemented the experiment in terms of its depth, width and kind of vegetations and calculated the reduction of pollutants loading. The experimental zone encompasses the watershed of Namhan River (Kyunggido Yangpyunggun Byungsanri). The region was divided into 5 land cover sectors : grass, reed, pussy willow, mixed(grass+pussy willow) and natural zone to compare effectiveness of vegetation. Water samples from four points have been collected in different depths. And the pollutant removal efficiency by sectors with different plant species was yielded through influent with one of each sample. And we obtained the correlation between the width of riparian buffer zone and the removal efficiency of pollutants. Using correlation result, the width of riparian buffer zones which needs to improve the water quality of river could be derived.

• The KIPS Transactions:PartB
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• v.17B no.6
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• pp.413-420
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• 2010

In this paper, we propose a scheme for dynamic allocating network bandwidth based on the playback buffer levels of the clients in a distributed mobile multimedia system. In this scheme, the amount of bandwidth allocated to serve a video request depends on the buffer level of the requesting client. If the buffer level of a client is low or high temporarily, more or less bandwidth will be allocated to serve it with an objective to make it more adaptive to the playback situation of this client. By employing the playback buffer level based bandwidth allocation policy, fair services can also be provided to the clients. In order to support high quality video playbacks, video frames must be transported to the client prior to their playback times. The main objectives in this bandwidth allocation scheme are to enhance the quality of service and performance of individual video playback such as to minimize the number of dropped video frames and at the same time to provide fair services to all the concurrent video requests. The performance of the proposed scheme is compared with that of other static bandwidth allocation scheme through extensive simulation experiments, resulting in the 4-9% lower ratio of frames dropped according to the buffer level.

• Analytical Science and Technology
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• v.27 no.6
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• pp.333-338
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• 2014

A simple and sensitive analytical method for fluoride in urine by ion selective electrode (ISE) method was presented. Traditional buffer for fluoride determination using ISE is acetate-based one. Researchers have pointed out some drawbacks of the buffer for fluoride ISE analysis, and some other buffers including citrate-ammonium buffer and MES buffer have been studied for accurate determination of fluoride in urine here. These buffers provided promising results in environmental field, and this author focused on overcoming the interference of co-existing aluminium. The results show that MES-CyDTA buffer gave the best recovery with accuracy of 95-97.5% and precision of 1.9-7.9% for reference sample of 1.8-7.8 mg/L fluoride in urine, with smaller amount of samples and shorter analysis time compared with the traditional method which used acetate buffer. The method was applied to field samples, and which showed urinary of $0.98{\pm}0.38mg/g$ creatinine for workers in electric cable manufacturing factory (n=15) and $0.59{\pm}0.30mg/g$ creatinine for non-exposed workers (n=12).

• International Journal of Computer Science & Network Security
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• v.23 no.1
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• pp.153-163
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• 2023

This study is situated in the context of intelligent transport systems, where in-vehicle devices assist drivers to avoid accidents and therefore improve road safety. The vehicles present in a given area form an ad' hoc network of vehicles called vehicular ad' hoc network. In this type of network, the nodes are mobile vehicles and the messages exchanged are messages to warn about obstacles that may hinder the correct driving. Node mobilities make it impossible for inter-node communication to be end-to-end. Recognizing this characteristic has led to delay-tolerant vehicular networks. Embedded devices have small buffers (memory) to hold messages that a node needs to transmit when no other node is within its visibility range for transmission. The performance of a vehicular delay-tolerant network is closely tied to the successful management of the nodes' transit buffer. In this paper, we propose a message transit buffer management model for nodes in vehicular delay tolerant networks. This model consists in setting up, on the one hand, a policy of dropping messages from the buffer when the buffer is full and must receive a new message. This drop policy is based on the concept of intermediate node to destination, queues and priority class of service. It is also based on the properties of the message (size, weight, number of hops, number of replications, remaining time-to-live, etc.). On the other hand, the model defines the policy for selecting the message to be transmitted. The proposed model was evaluated with the ONE opportunistic network simulator based on a 4000m x 4000m area of downtown Bouaké in Côte d'Ivoire. The map data were imported using the Open Street Map tool. The results obtained show that our model improves the delivery ratio of security alert messages, reduces their delivery delay and network overload compared to the existing model. This improvement in communication within a network of vehicles can contribute to the improvement of road safety.

• Journal of Information Processing Systems
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• v.2 no.2
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• pp.76-81
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• 2006

A PDA is used mainly for downloading data from a stationary server such as a desktop PC in an infrastructure network based on wireless LAN. Thus, the overall performance depends heavily on the performance of such downloading with PDA. Unfortunately, for a PDA the time taken to receive data from a PC is longer than the time taken to send it by 53%. Thus, we measured and analyzed all possible factors that could cause the receiving time of a PDA to be delayed with a test bed system. There are crucial factors: the TCP window size, file access time of a PDA, and the inter-packet delay that affects the receiving time of a PDA. The window size of a PDA during the downstream is reduced dramatically to 686 bytes from 32,581 bytes. In addition, because flash memory is embedded into a PDA, writing data into the flash memory takes twice as long as reading the data from it. To alleviate these, we propose three distinct remedies: First, in order to keep the window size at a sender constant, both the size of a socket send buffer for a desktop PC and the size of a socket receive buffer for a PDA should be increased. Second, to shorten its internal file access time, the size of an application buffer implemented in an application should be doubled. Finally, the inter-packet delay of a PDA and a desktop PC at the application layer should be adjusted asymmetrically to lower the traffic bottleneck between these heterogeneous terminals.

• Journal of Digital Contents Society
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• v.16 no.3
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• pp.381-388
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• 2015

TCP, which performs congestion control in congestion condition, is able to help a reliable transmission. However, packet loss can be increased because congestion window is increased by the time the packet is dropped in the process of congestion avoidance. In this paper, to solve the above problem, we propose a new congestion estimation based TCP congestion control scheme using the weighted average value of the RTT. After measuring a SRTT, which means the weighted average value of RTTs, at this point of time when a buffer overflow is occurred by an overloaded packet, the proposed scheme estimates the time, when the same SRTT is made in packet transmission, as a congestion time and then decreases the congestion window. The simulation results show that the proposed schem has a good performance in terms of packet loss rate and throughput when the packet loss due to buffer overflow is larger than that due to wireless channel.