• Preventive Nutrition and Food Science
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• 제4권1호
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• pp.33-37
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• 1999

The antimutagenic effects of buchu kimchi and Chinese cabbage kimchi and theri cytotoxic effects against human cancer cell line were investigated in the Salmonella typhimurium system and MTT assay, respectively. Leek and Chinese cabbage were aslo evaluated in the same system. Buchu kimchi was fermented at 15 $^{\circ}C$ for 4 days . Buchu kimchi samples showed somewhat higher antimutagenic effects against aflatoxin B1(AFB1) than CHinese cabbage kimchi in Salmonella typhimurium TA100 strain. There was no difference onthe antimutagenic activity according to the length of fermentation . Leek exerted stronger antimutagenicity against AFB1 than Chinese cabbage in the Ames assay. In MTT assay, 6-day fermented buchu kimchin revealed the highest cytotoxicity against AGS human gastric adenocarcinoma cells in which 62% and 82% of the inhibition were observed wiht the addition of 100ug, 400ug/well, respectively. Buchu kimchi samples caused 60~70% inhibition on the proliferation of HT-29 at 400ug/well. Leek exhibited higher antiproliferative effect against both AGS cells and HT-29 cells than Chinese cabbage in MTT assay. From these results, it is considered that buchu kimchi has stronger antimutagenic and in vitro anticancer effects than Chinese cabbage kimchi and the high inhibition rate of buchu kimchi probably results from leek, the major ingredient of buchu kimchi .

• 한국환경성돌연변이발암원학회지
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• 제21권1호
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• pp.51-56
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• 2001

Kimchi is a major Korean traditional fermented food, as a supplying source of vitamin and minerals which is prepared with various vegetables and condiments such as red pepper, garlic and salted fish etc. There are many types of Kimchi depending on the ingredients and preparation methods used. To investigate the clastogenicity and anticlastogenicity of Baechu (Chinese cabbage) Kimchi and Buchu (leek, Allium odorum) Kimchi in mouse, it was performed acridine orange supravital staining of micronucleus (AOSS-MN) assay using mouse peripheral reticulocytes. Baechu Kimchi and Buchu Kimchi were cultivated by organic agricultural technique, and Kimchi samples were prepared by methanol extraction and lyophilization. First of all, it was studied the clastogenicity of two Kimchi samples themselves (250-1,000 mg/kg) after oral adminstration in mouse. And also to study the anticlastogenic effect of oral administration of Kimchi samples, mitomycin C (MMC, 1 mg/kg, i.p.) was used as micronucleus inducing agent in this study. Dosing scheme was performed as simultaneous (co-treatment), 3 hr before (pre-treatment) and 3 hr after (post-treatment) with MMC treatment. Two Kimchi samples in the range of 250-1,000 mg/kg did not reveal any clastogenic effect in AOSS-MN assay in mouse. They also revealed anticlastogenic effects in post-treatment of Baechu Kimchi (1,000 mg/kg), and in pre-treatment of Buchu Kimchi (500 and 1,000 mg/kg) with statistical significance. The anticlastogenic effect revealed 1 and 6 hr after treatment of Baechu Kimchi, and Buchu Kimchi with 3 and 6 hr pretreatment. Consequently, it is suggested that antimutagenic and anticlastogenic mechanisms of Baechu and Buchu Kimchi in vivo attributed to sipindle formation and kinetic behavior of mutagens such as absorption and metabolism etc.

• Preventive Nutrition and Food Science
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• 제1권1호
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• pp.23-29
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• 1996

This study was conducted to investigate the changes in chemical, microbial and sensory characteristics, and antimutagenicity of buchu(leek, Allium odorum) kimchi during fermentation at 15$^{\circ}C$. Reducing sugar contents and pH of buchu kimchi were decreased during the fermentation. The reduction rate of reducing sugar of glutinous rice paste and shrimp added buvhu kimchi(GSBK) was faster than those of control buchu kimchi(CBK) and glutious rice paste added bucku kimchi(GBK). Acidity increased rapidly until 4 days,a nd optimum acidity (0.6%) of bucku kimchi was reached within 2 day. Also total bacterial and lactic acid bacterial counts greatly increased after 4 days of the fermentation. The numbers of lactic acid bacteria after 8 day- fermentation in CBK and GSBK, and 10 day-fermentation in GBK were the highest values, 4.5$\times${TEX}$10^{8}${/TEX} CFU/ml, 4.8$\times${TEX}$10^{8}${/TEX} CFU/ml and 6.1$\times${TEX}$10^{8}${/TEX} CFU/ml, respectively. In the sensory evaluation, appearance of sample was good at 0 day, taste from overall quality of buchu kimchi were asquired the highest values at 6th day. The methanol extracts from buchu kimchi(GBK) showed antimutagenicity against aflatoxin {TEX}$B_{1}${/TEX}({TEX}$AFB_{1}${/TEX})in Salmonella typhimurium TA100. The inhibition ration were 58~69% with treatment of the 5% methanol extracts, and when the adding concentration increased the effect increased.

• 한국식품영양과학회지
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• 제33권2호
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• pp.291-298
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• 2004

김치에는 여러 가지 항산화 물질들이 많이 함유되어 있으므로 김치의 섭취는 생체조직의 노화억제 효과가 있을 것으로 기대된다. 본 연구에서 배추김치, 갓김치, 부추김치를 동결건조하여 항산화물질의 함량을 측정한 결과 갓김치와 부추김치의 클로로필 함량이 가장 높았고 $\beta$-카로텐과 ascorbic acid 함량은 갓김치에서 가장 높았다. Hairless mouse에게 10% 동결건조 김치식이를 공급하여 20주간 사육한 후 간에서 활성산소종의 생성량을 측정한 결과 superoxide anion과hydroxyl radical 함량은 유의적인 차이가 없었고 hydrogen peroxide 함량은 갓김치군과 부추김치군에서 유의적으로 낮게 나타나 이들 김치가 활성산소를 소거하는데 효과가 있는 것으로 나타났다. 간 균질액에서 지질과산화물을 측정한 결과 대조군에 비해 김치섭취군에서 지질과산화가 낮게 나타났으며, 특히 갓김치 와 부추김 치군의 효과가 우수하였다. 피부에서 지질과산화물 함량은 김치섭취군들이 대조군보다 낮게 나타났으며, 부추김치가 유의적으로 지질과산화를 억제하였다. 김치섭취가 광산화에 미치는 영향을 규명하기 위해 간 및 피부조직에 자외선을 조사한 후 조직산화 정도를 측정한 결과 간과 피부의 지질과산화물 함량은 갓김치군과 배추김치군에서 대조군보다 낮아졌으나 부추김치군에서는 높게 나타났다. 즉, 자외선 조사 후 갓김치군과 배추김치군은 대조군보다 광산화가 억제되었으나 부추김치군에서는 산화가 촉진되었다.

• 한국식품영양과학회지
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• 제33권5호
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• pp.785-790
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• 2004

배추김치, 갓김치, 부추김치의 용매획분 추출물을 제조한 뒤 항산화관련 물질들의 함량을 측정한 결과 카로테노이드는 갓김치의 hexane층에서, 클로로필 함량은 부추김치의 hexane층에서 가장 높았고, 비타민 C함량은 갓김치의 EtOAc층과 BuOH층에서 가장 높게 나타났다. 총 폐놀화합물은 부추김 치의 CH$_2$Cl$_2$층과 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 갖김치 EtOAc층과 CH$_2$Cl$_2$층에 많이 함유되어 있었다. DPPH법을 이용한 유리기 소거능을 조사한 결과 부추김치 CH$_2$Cl$_2$층과 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 갓김치 EtOAc층에서 효과가 큰 것으로 나타났다. 김치종류별 용매획분을 hairless mouse의 피부균질액에 첨가하여 자외선 B를 조사한 후 지질과산 화물 함량을 측정한 결과 부추김치의 CH$_2$Cl$_2$층과 EtOAc층, 배추김치, 갓김치의 EtOAc층에서 낮게 나타나 항산화성이 높은 획분에서 피부의 광산화 억제효과가 큰 것을 알 수 있었고 김치 용매획분을 크림에 첨가하여 hairless mouse의 등에 직접 도포한 후 자외선 B를 조사하였을 때 대조군에 비해 갓김치의 hexane층, 부추김치의 hexane층과 BuOH층을 제외한 모든 용매획분이 피부홍반의 생성을 억제하였고 특히 갖김치 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 부추김치 물층에서 피부보호효과가 큰 것으로 나타났다.

• Preventive Nutrition and Food Science
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• 제4권4호
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• pp.255-259
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• 1999

The anticarcinogenic effects of the methanol extracts from leek (buchu in Korean) kimchi and Korean cabbage kimchi were evaluated using cytotoxicity and transformation tests in C3H/10T1/2 cells. Various fractions of the 6-day fermented leek kimchi at 15$^{\circ}C$, hexane, methanol soluble, dichloromethane, ethyl acetate, butanol and aqueous fraction, were also studied in the same system. The inhibitory effect of the leek kimchi(6-day fermented at 15$^{\circ}C$, pH 4.29) was higher than that of the Korean cabbage kimchi(4-day fermented at 15$^{\circ}C$, pH 4.21) on the cytotoxicity induced by 3-methylcholanthrane (MCA) in the C3H/10T1/2 cell system. While the MCA-treated culture(control) formed 21.0 foci of type II plus III in C3H/10T1/2 cells, 100$\mu\textrm{g}$/ml of the methanol extract of the leek kimchi and that of the 4-day fermented Korean cabbage kimchi treated cultures reduced the formation of type II plus III foci to 7.4 and 11.3, respectively. Among the fractions of the leek kimchi, the dichloromethane fraction showed the highest inhibitory effect on MCA-induced cytotoxicity in C3H/10T1/2 cells. Fifty $\mu\textrm{g}$/ml of dichloromethane fraction from the leek kimchi suppressed the MCA-induced cytotoxicity by 77%. On the transformation test using MCA, the dichloromethane fraction considerably reduced the formation of type II plus III foci, especially thpe III foci. When 50$\mu\textrm{g}$/ml of dichloromethane fraction from the leek kimchi was treated, the numbers of type III foci mediated by MCA were decreased to 1.7 compared to 10 for the control. These results indicate that leek kimchi has stronger anticarcinogenic effects than Korean cabbage kimchi and that the dichloromethane fraction of the leek kimchi may contain the major compound(s) that suppress the carcinogenesis in the eukaryotic cells.

• 대한가정학회지
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• 제36권9호
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• pp.127-137
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• 1998

The main purpose of this study is to provide a basic knowledge of Kimchi preferred by high school students and to improve high school students' Kimchi intake. A questionnaire survey was conducted on 1,056 high school students in Taegu area. The results were as follows: 82% of the students had an affirmative opinion of Kimchi intake and they regarded Kimchi as one of our traditional, healthful and nutritious foods. The most well known Kimchi is as follows: Kkaktugi, Baechu Kimchi, Mul Kimchi, Chonggak Kimchi, Pa Kimchi, Dongchimi, Kkaennip Kimchi and Buchu Kimchi. The students preferred the white stems of the Chinese cabbage. Boy students preferred taste of fresh prepared Kimchi but girl students more preferred properly fermented Kimchi than the other. Their favorite ingredients were Korean radishes, oysters, green onion and carrots, also their favorite spices were red pepper powder, anchovies sauce, garlic and sugar in the order. Among those spices, boy students more preferred anchovies sauce than girl students. The students regarded market Kimchi as one of convenient and time-saving, but less quality and insanitary foods.

• 한국조리학회지
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• 제12권4호
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• pp.199-212
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• 2006

The objective of this study were to examine the consumption pattern and awareness of Kimchi, which is our traditional food, preference for Kimchi, and the way of quality improvement in order to understand the recognition about the position and importance of Kimchi. The results are as follows. As for the recognition about regional way of making Kimchi, it turned out that married women in Seoul and Daejeon don't know their own ways of making Kimchi, and those in Daejeon don't know it more than those in Seoul. The rate of using Kimchi made at home was higher in Daejeon than in Seoul, and the rate of using Kimchi in both ways turned out higher in Seoul than in Daejeon. The reason why they use home-made Kimchi was that they generally prefer their own specific tastes both in Seoul and in Daejeon. Daily Kimchi ingestion was higher in Daejeon than in Seoul. Besides, the recognition about the superiority of our traditional Kimchi turned out high both in Seoul and in Daejeon. The survey of preferring Kimchi taste has been found that well pickled and proper seasoned Kimchi were most preferred both in Seoul and in Daejeon. Preference rate of various kinds of Kimchi came in the following order: Baechu Kimchi, Mu Kimchi, Oisobaki, Pa-buchu Kimchi in the Seoul area while Baechu Kimchi, Mu Kimchi, Pa-buchu kimchi, Oisobaki in the Daejeon area. In general, a sort of Kimchi stew was most popular as a dish using Kimchi with 63.8% both in Seoul and in Daejeon. It has been found in Seoul as well as in Daejeon that Kimchi served at a restaurant was usually untasty in comparison with Kimchi at home. The rate of preferring Kimchi on the markets in Seoul and in Daejeon as a whole came in the following order: Baechu Kimchi, Chongkak Kimchi, Baek kimchi People thought both in Seoul and in Daejeon that materials and cleanness were top priority when choosing Kimchi on the market. According to the result as above, recognition rate of Kimchi in the Seoul and Daejeon areas was shown rather low. Consequently, a further detailed research on Kimchi is thought to be needed. As the recognition of Kimchi increases, Kimchi consumption will increase accordingly. In order to prevent adult diseases, I think we should increase the consumption of Korean traditional fermentation food including Kimchi by increasing recognition of importance of nutrition, function, superiority, and the role of fermentation of Kimchi.

• Preventive Nutrition and Food Science
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• 제7권3호
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• pp.250-254
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• 2002

The anticancer effects of leek (buchu in Korean) kimchi were evaluated in the human cancer cells: AGS gastric adenocarcinoma cells, HT-29 human colon adenocarcinoma cells and HL-60 leukemia cells. The leek kimchi (fermented for 6 days at 15$^{\circ}C$) was fractionated into 7 groups: methanol extract, hexane extract, methanol soluble extract MSE), dichloromethane (DCM) fraction (fr.), ethyl acetate fr., butanol fr. and aqueous fr. Most of the leek kimchi tractions inhibited the growth of AGS and HT-29 cancer cells in a dose dependent manner. In particular, the DCM fr. showed the highest inhibitory effect among the tractions. Treatment with the DCM fr. (0.1 mg/mL) reduced the survival rates of AGS and HT-29 cancer cells to 19% and 37% of the controls, respectively. Moreover the DCM fr. of the leek kimchi arrested G2/M phase in the cell cycle and induced apoptosis in HL-60 human promyelocytic leukemia cells. These results indicate that the leek kimchi exerted an anticancer effect on those human cancer cells, and that the DCM fr. arrested G2/M phase in the cell cycle and induced apoptosis in the leukemia cells.

• 한국식품조리과학회지
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• 제13권3호
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• pp.259-265
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• 1997

대구 ·경북 지역 초등학생들의 김치에 대한 의식 및 선호도를 조사한 결과는 다음과 같다. 1. 조사 대상의 57.7%는 김치를가능하면 먹는 것이 좋다고 생각하였고 26.1％는 반드시 먹어야 한다고 응답하여 83.8%가 김치를 섭취해야 한다고 인식하고 있었다. 또한 김치를 영양식품(51.3%), 전통식품(2:2.9%)으로 인식하는 비율도 높아 김치의 우수성을 이해하고 있었다. 2. 김치종류별 인지도는 배추김치(83.0%), 깍두기(65.0%), 나박김치 (82.2%), 총각김치(58.4%), 깻익김치(43.2%), 부추김치(39.1%)의 순으로 높았고 어머니들도 배추김치(92.2%), 총각김치(85.8%), 깍두기(83.Bfa), 나박김치(82.2%), 부추김치(77.1%), 깻잎김치(75.6%)의 순으로 인지도가 높아 초등학생과 비슷한 경향이었다. 3. 김치에 대한 선호 실태는 매우 좋아한다가 22.8%였고 좋아한다가 32.7%로서 55.5%의 초등학생들이 김치를 좋아하고 있었다. 초등학생들이 선호하는 김치는 배추김치(74.0%), 깍두기(56.6%), 총각김치(49.0％), 나박김치(46.9%), 오이소박이(17.5%) 등이었고 배추김치중 배추의 줄기부분(47.6%)을 가장 좋아하였다. 숙성정도는 적당히 잘 익은 김치(43.9%)와 갓 담은 김치(41.9%)를 선호하였다. 4. 김치를 재료로 하여 만든 음식으로는 김치볶음밥, 김치찌개, 김치전, 김치비빔밥, 김치김밥, 김치만두 등의 순으로 좋아하였다. 5. 어머니의 지도형태와 초등학생의 김치에 대한 의식 및 선호도는 유의한 차이를 보였으며(p < .001)자주 먹도록 권유하거나 조리 방법을 바꾸어 주는 것이 먹이려고 강요하거나 전혀 간섭하지 않는 것보다 좋은 영향을 미쳤다. 6. 김치를 싫어하는 이유는 매운 맛, 양념 냄새를 지적하였고 생강, 청각, 마늘, 미나리, 파 등의 부재료는 싫어하였다. 7. 김치에 대한 바램은 김치 맛을 되도록 덜 맵고 달콤하게 해 달라는 요구가 많았고 배, 오징어, 글, 사과, 오이 등의 부재료를 김치에 첨가해 주기를 바라고 있었다.