• Title/Summary/Keyword: brown spots

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Effect of Scirpi rhizoma Ethanol Extract on Skin Whitening in an Animal Model of Brown Guinea Pigs (기니아 피그 동물모델에서 삼릉 에탄올추출물의 미백 효과)

  • Ko, Ju-Young;Choi, Kyung-Hwa;Kim, Young-Chul
    • Environmental Analysis Health and Toxicology
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    • v.24 no.3
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    • pp.219-229
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    • 2009
  • The purpose of this study is to evaluate the skin whitening effect of Scirpi rhizama ethanol extract (SREE) in an animal model. For the experiment of the study, three brown guinea pigs weighing about 450 g to 550 g were exposed to ultraviolet-B rays on the backs at 500 $mJ/cm^2$ once a week, three consecutive weeks and the total quantity of light was 1,500 $mJ/cm^2$. The artificial tanning spots were divided into six different groups including normal (N), control (C), vehicle control (VC), positive control (PC), experimental 1 (E1, 1% SREE), experimental 2 (E2, 2% SREE) groups. Then, 30 ${\mu}L$ of SREE was transdermaly applied on the artificial tanning spots twice a day and 5 days a week for 8 weeks. With the result of a gross observation, it was found that the degree of pigmentation became apparently thinner in the group applied with E2, compared to the control or the vehicle control group. The melanin index of E2 group was significant lower than the control or the vehicle control group. In the observation with a light microscope, it was found that the degree of melanin pigmentation and S-100 protein expression considerably decreased in the groups applied with SREE, compared to the control or the vehicle control group. With the numerical analysis of melanin pigmentation and S-100 protein expression by using image-analysis software, it was found that the tendency was coincide with the results of microscopic observation.

Occurrence of Eggplant Scab Caused by Cladosporium cucumerinum in Korea

  • Kwon, Jin-Hyeuk;Kang, Soo-Woong;Park, Chang-Seuk
    • The Plant Pathology Journal
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    • v.15 no.6
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    • pp.345-347
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    • 1999
  • A scab disease on eggplant (Solanum melongena L. cv. Chukyang) in plastic film houses around Kimhae area in Korea during the winter season of 0998-1999. The disease started on leaves with small dark brown spots which were gradually expanded to 1 to 3 mm diameter lesions. Later, the central parts of the lesions became collapsed and detached to make holes. Dark brown mold was grown out of the lesions on the lower side of leaf. Numerous conidia were produced on the lower side of leaf. Numerous conidia were produced on the diseased leaves and appeared to be readily dispersed in the air. A fungus was isolated from the diseased leaves, and tested for Koch's postulates to prove the causal agent of the desease. The isolated fungus grew on potato dextrose agar, forming greenish black to pale brown colonies. Conidia were ellipsoidal, fusiform or subspherical, mostly one-celled but occasionally septated, and formed in long branched chains on the erected conidiophores which were pale olevaceous brown and variable in length between 12.4 and $393.4\mu\textrm{g}$. The fungus was identified as Cladosporium cucumerinum Ellis Arthur based on the above morphological characteristics examined. The optimum temperature for mycelial growth and conidial formation was about 20 to $25^{\circ}$. In addition to cucumber, the fungus was also pathogenic to watermelon, pumpkin and oriental melon. This is the first report on the scab disease of eggplant in Korea.

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Inhibitory effects of 15 mushroom culture extracts on the growth of Alternaria alternata causing potato brown spot (감자갈색잎점무늬병균(Alternaria alternata)에 대한 버섯 배양액 추출물 15종의 생장 억제 효과)

  • Hyon Jin Park;Ha Thi Kim Nguyen;Sook-Young Park;Jaehyuk Choi
    • Journal of Mushroom
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    • v.21 no.4
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    • pp.195-199
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    • 2023
  • The fungus Alternaria alternata, responsible for causing brown to black spotting on numerous fruits and vegetables globally, was identified in 2022 as the causative pathogen of brown spot disease in potatoes in Korea. In pursuing potential inhibitors against A. alternata growth, we evaluated 15 mushroom culture filtrates: eight from Trametes spp. and seven from Polyporus spp., known for their antibacterial and anticancer properties. Antifungal activity was assessed by exposing each filtrate to A. alternata on a paper disc. Four filtrates displayed inhibitory action against the fungus, albeit with mild effects. Our findings highlight the potential of Trametes and Polyporus fungi as emerging antifungal candidates, offering promise in preventing potato brown spots.

Zonate Leaf Spot of Sorghum Caused by Gloeocercospora sorghi in Korea

  • Chul Heo;Kim, Jung-Nyo;Hyun, Ik-Hwa;Heo, Noh-Youl
    • The Plant Pathology Journal
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    • v.15 no.4
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    • pp.242-246
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    • 1999
  • Gloeocercospora sorghi caused the zonate leaf spot on sorghum (Sorghum bicolor Moench) in fields in Korea. The zonate spots were conspicuous on sorghum leaves as circular, reddish purple bands alternating with tan or straw-colored areas, which formed a coarsely zonate pattern. The lesions often occurred in semicircular patterns along the margin of leaves. The fungus produced sporodochia on the surface of infected leaves in a moist chamber. The conidia, born in a pinkish to salmon-colored slimy matrix, were hyaline, elongate to filiform, straight or slightly curved, 3- to 17-septate, and of variable lenght (28-197 x 1.6-$3.4\mu\textrm{m}$). G. sorghi isolated from diseased leaves of sorghum caused characteristic reddish-brown water-soaked leaf spots when inoculated to seedlings.

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Bacterial Black Stem Rot on Angelica acutiloba Caused by Xanthomonas campestris

  • Han, Kwang-Seop;Shim, Myoung-Youg;Oh, In-Seok;Han, Kyu-Hung;Park, Jae-Eul
    • The Plant Pathology Journal
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    • v.18 no.1
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    • pp.54-55
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    • 2002
  • Soaked black rot symptom was observed on the stem of Angelica acutiloba from July to August 2000 at Kumsan, Chungnam in Korea. This disease usually occurred under humid and high temperature conditions. The lesions on the stem appeared as soft rot with brown elliptical spots, which developed into large black spots at a later stage. When the bacterial isolates from the diseased plants were inoculated onto healthy plants by artificial needle prick method, symptoms similar to that observed in the fields developed. According to the cultural characteristics and pathogenicity of the isolates on the host plant the causal bacterium was identified as Xanthomonas campestris. This study proposed that the disease be named "bacterial black stem rot of A. acutiloba"loba".

Occurrence and Distribution of Bacterial Canker of Red Pepper Caused by Clavibacter michiganensis subsp. michiganensis (Clavibacter michiganensis subsp. michiganensis에 의한 고추 궤양병)

  • 이승돈
    • Plant Disease and Agriculture
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    • v.5 no.2
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    • pp.105-110
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    • 1999
  • Bacterial leaf spot by Xanthomonas campestris pv. vesicatoria has been known to cause serious problem in red pepper in Korea. However recent survey showed that most smptoms in the leaves were mixed with two different symptoms one was leaf spot and the other was canker. bacteria isolated from canker were identified as Clavibacter michiganensis subsp. michiganensis on the basis of biochemical and physiological characteristics. The causal bacteria were non-motile rod-shaped and Gram-positive. The lesions on pepper leaves appeared at first as small blisters or pimple-like white spots which enlarged in size at a later stage. The centers of some of the spots became necrotic and brown and were surrounded by a white halo. Pathogenicity tests were performed on pepper cv. Alchan seedling by spraying of bacterial suspension. During 1997 and 1998 total 17% of 527 fields surveyed were infected by C. michiganensis subsp. michiganensis. The canker of red pepper caused by C. michiganensis subsp. michiganensis was first identified in this study in Korea, and new name "gueyangbyung" was tentatively given to the disease.

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First Report of Leaf Rust Caused by Puccinia caricis in Farfugium japonicum in Korea

  • Yun, Yeo Hong;Kwon, Hyuk Woo;Ahn, Hong Seok;Kim, Seong Hwan
    • Mycobiology
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    • v.43 no.3
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    • pp.351-353
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    • 2015
  • Farfugium japonicum is used in traditional medicine and as an edible herb in China and Korea. In July 2013, leaf spots were observed in F. japonicum seedlings at Ulleung Island, Gyeongsangbuk Province, Korea. Early symptoms on the leaf adaxial surface included roughly circular yellow spots that later developed brown, necrotic centers. The aecia were hypophyllous, cupulate, yellowish, $180{\sim}430{\mu}m$ in diameter, clustered, and erumpent with a peridium with a recurved margin. The aeciospores were globoid, $14{\sim}17{\times}13{\sim}16{\mu}m$, light yellow or colorless, and densely verrucose. The 28S rDNA sequence of the isolate was identical to each other and shared 99% identity with Puccinia caricis. This is the first report of rust caused by P. caricis in F. japonicum in Korea or elsewhere in the world.

Anthracnose of Gardenia (Gardenia jasminoides for, grandiflora Makino) Caused by Glomerella cingulata

  • Kim, Gyoung-Hee;Lee, Jae-Goon;Hur, Jae-Seoun;Koh, Young-Jin
    • The Plant Pathology Journal
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    • v.18 no.4
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    • pp.228-230
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    • 2002
  • Anthracnose symptoms caused by Glomerella cingulata were observed on leaves and stems of gardenia in Sunchon, Jeonnam in Korea in 2000. Symptoms on infected plants typically appeared as irregularly circular, dark-brown ring spots and water-soaked brown lesions. Based on cultural and morphological characteristics, the fungus (G-00-03 isolate) from the diseased plants was identified as Colletotrichum gloeosporioides, and its teleomorph stage was Glomerella cingulata. Healthy gardenia artificially inoculated with fungal spores showed anthracnose symptom 7 days after inoculation. This is the first report of gardenia anthracnose caused by Glomerella cingulata in Korea.

Bacterial Soft rot of Kalanchoe blossfeldiana by Erwinia herbicola in Korea (Erwinia herbicola 의한 Kalanchoe blossfeldiana세균성무름병)

  • 최재을;이은정
    • Research in Plant Disease
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    • v.6 no.1
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    • pp.15-18
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    • 2000
  • A new bacterial disease was found on leaves of Kalanchoe blossfeldiana plant grown under vinyl-house condition in winter of 1998 in Taejeon. the first symptoms of the disease are the appearance of the water-soaked and light brown spots. Later they become soft rot with brown color. Causal bacteria were isolated from diseased tissues and the same symptoms as the natural infection were developed on Kalanchoe blossfeldiana leaves by needle-prick inoculation. The causal bacterium was identified Erwinia hervicola by its bacteriological characteristics. This is the first reported of this bacterium to occur on kalanchoe blossfeldiana plant in Korea. Therefore, we proposed to name the diseases as \"bacterial soft rot of Kalanchoe blossfeldiana\" by E. herbiocla.

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First Report of Fusarium subglutinans Causing Leaf Spot Disease on Cymbidium Orchids in Korea

  • Han, Kyung-Sook;Park, Jong-Han;Back, Chang-Gi;Park, Mi-Jeong
    • Mycobiology
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    • v.43 no.3
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    • pp.343-346
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    • 2015
  • In 2006~2010, leaf spot symptoms, that is, small, yellow spots that turned into dark brown-to-black lesions surrounded by a yellow halo, were observed on Cymbidium spp. in Gongju, Taean, and Gapyeong in Korea. A Fusarium species was continuously isolated from symptomatic leaves; in pathogenicity testing, isolates caused leaf spot symptoms consisting of sunken, dark brown lesions similar to the original ones. The causal pathogen was identified as Fusarium subglutinans based on morphological and translation elongation factor 1-alpha sequence analyses. This is the first report of F. subglutinans as the cause of leaf spot disease in Cymbidium spp. in Korea.