• International Journal of Industrial Entomology and Biomaterials
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• 제2권2호
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• pp.123-127
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• 2001

In order to introduce bivoltine races in a tropical country like India, it is necessary to have stability in cocoon crop under high temperature environments. Unlike any temperate country like Japan, the rearing conditions/environment, climatic conditions, quality of mulberry leaf and incidence of diseases are unpredictable in India. Geneticists and breeders of all the sericultural countries have experienced the influence of environment during the process of breeding. in order to select efficiently the breeds with high temperature tolerance, it is verb important to analyse clearly the heritability nature of high temperature tolerance. In light of the above, the present study was undertaken to determine the effect of high temperature treatment of (A) $35{\pm}1^{\circ}C$ and 85${\pm}$5% RH for 24 hrs continuously, (B) $35{\pm}1^{\circ}C$ and 85${\pm}$5% RH for 48 hrs continuously and (C) the control (25${\pm}1^{\circ}$ and 65${\pm}$5% RH in the normal rearing condition from the $3^{rd}of5^{th}$ instar on the pure races such as Moria, Nl37 and Cl46 as well as their Fl hybrids. The overall performance indicate that the hybrids are mare tolerant than the pure races. it was also observed that the overall performance declined in those batches where 48 hrs treatment was given. The most interesting observation noticed in this study was that there was maternal effect regarding temperature tolerance as evident from the better performance of those hybrids where the female parent used was more tolerant as pure race.

• 한국작물학회지
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• 제53권3호
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• pp.297-302
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• 2008

Application of genotype by environment ($G\;{\times}\;E$) interaction would be used for identifying optimum test condition of the varietal adaptation in the establishment of breeding purpose. Yield and yield components were used to perform additive main effect and multiplicative interaction (AMMI) analysis. Significant difference for $G\;{\times}\;E$ interaction were observed for all variable examined. For yield, 0.18 of total sum of squares corresponded to $G\;{\times}\;E$ interaction. Correlation analysis was carried out between genotypic scores of the first interaction principal component axis (IPCA 1) for agronomic characters. Significant correlations were observed between IPCA 1 for yield and capsule bearing stem length (CBSL), number of capsule per plant (NOC). The biplot of grain yield means for IPCA1 which accounted for 34% of the variation in total treatment sums of squares showed different reaction according to $G\;{\times}\;E$ interaction, genotypes and environments. Taegu showed relatively lower positive IPCA1 scores, and it also showed smaller coefficient variation of yield mean where it is recommendable as a optimal site for the sesame cultivar adaptation and evaluation trial. In case of variables, Yangbaek and M1 showed relatively lower IPCA1 scores, but the score direction showed opposite each other on the graph. Ansan, Miryang1, Miryang4, and Miryang6 seemed to be similar group in view of yield response against IPCA1 scores. These results will be helpful to select experimental site for sesame in Korea to minimize $G\;{\times}\;E$ interaction for the selection of promising genotype with higher stability.

• 유기물자원화
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• 제11권1호
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• pp.92-96
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• 2003

본 연구는 하수슬러지 지렁이 퇴비화 장치 개발을 목적으로 재래식 지렁이 이용기술의 문제점 개선 및 지렁이 생리 생태를 이용하여 제작된 소규모 지렁이 퇴비화 장치의 성능평가을 통한 현장 적용 가능한 장치 설계 및 운영지표 설정을 위한 실험 결과 실험장치 성능평가에서는 하수슬러지 급이시 입자크기와 먹이 공급 층의 높이가 각각 5mm 크기와 10cm 두께로 급이 할 때 처리율이 가장 높았으며, 지렁이 적정 밀도는 20~25kg${\cdot}$지렁이/50kg${\cdot}$하수슬러지로 나타났다. 또한 분변토 분리효율은 지렁이 사육상 높이를 80cm으로 운영했을 때 분리효율이 가장 높게 나타났으며, 일 2톤의 하수슬러지 처리가 가능한 $5{\times}3{\times}1m$ 크기의 지렁이 퇴비화 장치 설계가 가능하였다.

• 한국환경과학회지
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• 제11권4호
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• pp.309-317
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• 2002

In this study, water protection reservoir is selected as the target which is located at the estuary of Taehwa river to analyze and examine the effects of hydraulic structure on river environment. This study examined the water quality variation characteristics among many effects of hydraulic structure on river environment before and after removal of the sediment protection reservoir when low flow is yielded. This study aims at the definition of factors which cause the change of ecological environment of river due to the effects of the sediment protection reservoir, and the proposal of the direction of environmental friendly river space development through the comparison of stream variation conditions(depth, velocity, and etc.) and riverbed variation characteristics with ecological depth condition of Taehwa-river's channel for each representative species of fish and examination those. Firstly, from the examination result of water quality when low flow is yielded before and after removal of the sediment protection reservoir for problems about water quality of river due to flow amount decrease in river, it is found that DO decreases about 0.78~0.86ppm at the lower stream of Myeongchon-gyo, and BOD decreases about 0.06~0.24ppm from right upper stream to the direction of estuary when the sediment protection reservoir is removed. It is known from the above that there is some improvement of water quality from the lower stream of Taehwa-gyo to the estuary in case of removal the sediment protection reservoir. Nextly, it is thought that the effects on ecosystem due to water depth and draw down in channel is not serious on the basis of the examination of water quality analysis result according to removal of sediment protection reservoir and hydraulic depths for reservation of ecosystem, these are 10~40cm for breeding season, 10~50cm for fry period, and 10~100cm for adult period of the representative species of fish in Korea.

• 대한의생명과학회지
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• 제26권2호
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• pp.114-119
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• 2020

Of the various types of mice used for genome editing, conditional knock-out (cKO) mice serve as an important model for studying the function of genes. cKO mice can be produced using loxP knock-in (KI) mice in which loxP sequences (34 bp) are inserted on both sides of a specific region in the target gene. These mice can be used as KO mice that do not express a gene at a desired time or under a desired condition by cross-breeding with various Cre Tg mice. Genome editing has been recently made easy by the use of third-generation gene scissors, the CRISPR-Cas9 system. However, very few laboratories can produce mice for genome editing. Here we present a more efficient method for producing loxP KI mice. This method involves the use of an HDR vector as the target vector and ssODN as the donor DNA in order to induce homologous recombination for producing loxP KI mice. On injecting 20 ng/µL of ssODN, it was observed that the target exon was deleted or loxP was inserted on only one side. However, on injecting 10 ng/µL of the target HDR vector, the insertion of loxP was observed on both sides of the target region. In the first PCR, seven mice were identified to be loxP KI mice. The accuracy of their gene sequences was confirmed through Sanger sequencing. It is expected that the loxP KI mice produced in this study will serve as an important tool for identifying the function of the target gene.

• 한국환경복원기술학회지
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• 제8권5호
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• pp.10-18
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• 2005

The research was carried out to define the morphological and growth characteristics of Poa pratensis L.(Kentucky bluegrass) 'Sewon' in native to Korea. Poa pratensis 'Sewon' was collected at the terrace land on the river in Guiman-ri, Cheongcheon-myeon, Goesan-gun, Chungcheongbuk-do on May 5, 1995. The collected Poa pratensis 'Sewon' was grown under non-mowing naturally growing condition. The morphological characteristics of Poa pratensis 'Sewon' are as follows. : The height of plant was 21.6cm but was increased to 33cm at the flowering time. The height of first node from the base was 5.0cm and the length and width of leaf were 9.6cm and 4.1cm, respectively. The length of inflorecences was 37.8cm and the length of a spike was 7.3cm. Based on these morphological characteristics, Poa pratensis 'Sewon' was defined to dwarf~medium and coarse texture type Kennturky bluegrass. 1 to 9 scale of visual quality, visual color and visual density were measured. Poa pratensis 'Sewon' showed best turf qualities among native Kentucky bluegrasses. In spring and fall growing season, Poa pratensis 'Sewon' were measured to be excellent performances for turf qulities similar to foreign excellent quality cultivars such as Midnight, Nuglade and Brilliant Kentucky bluegrasses. But turf quality of Poa pratensis 'Sewon' decreased by plant disease according to temperature increase in summe compared to foreign excellent quality cultivars. So, Poa pratensis 'Sewon' should be improved of disease tolerent quality by incessant breeding work in order to compete foreign excellent quality cultivars.

• 한국환경복원기술학회지
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• 제12권3호
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• pp.142-163
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• 2009

Taebaek city is located in the mountainous plateau area therefore the major industry was coal industry. According to dramatically declining of the major industry, Taebaek city is need to alternative industry which associated with considering the geographical characteristics of natural tourism resources for increasing the local economy. On the basis of these reasons, this study can be suggested ecological planning and mitigation of deterioration technique about the these study site. That is the reserved area for mountainous experience theme park. As the results of environmental ecology assessment are following as; The natural ecosystem areas, multi-layer structure forest such as forest of Pinus densiflora, forest of Quercus mongolica and deciduous forest have a high value of nature, diversity and potential. In addition, wild bird habitats were important area as a inhabitation, breeding, feeding and hiding. Therefore, on these areas should be preserved. Also, it needs to conserve on there such as more than three types of wild bird inhabitate areas, the fringe of high biological diversity, the wetland that have got good vegetation condition and the function of amphibia, reptiles crossing. In addition, inhabitation, the waterway of wetland form that have got wide waterside width needs to conserve. In conclusion, on the basis of analysis results such as conditions of plan, environment, ecological assessments, survey informations are able to suggest the connectivity of the axis of forest and management plan. Moreover, in the part of forest restoration plan, we suggest the plan of transplant for a compensation of damaged forest by land use.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1998년도 21세기 한반도 농업전망과 대책(한국작물학회.한국육종학회 공동주관 심포지움 회보)
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• pp.313-335
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• 1998

If the atmospheric concentrations of greenhouse gases double, the annual temperature increase in mean surface temperature relative to 1990 will be about 2.0 to $2.5^{\circ}C$ and the annual precipitation increase about $15{\%}$ by 2100 in Korea. When the temperature rises $2^{\circ}C$, the annual temperature will be $13^{\circ}C,\;15^{\circ}C,\;and\;16^{\circ}C$ in Western Central, Yeongnam Basin, and Southern Coastal respectively. Consequently the crop period could be prolonged $10{\~}29$ days. In the case of gradual global warming, annual crops could be adapted to the changed environment by breeding, and the perennial crops should be shifted to ether area. If global warming happens suddenly over the threshold of atmospheric greenhouse gases concentration, then we shall have disturbance of ecosystem. When $2^{\circ}C$ higher than present, the optimum flowering date of rice plant delayed about 10 days, so it may not possible to adopt the fate with present japonica rices, therefore, the recommended characteristics of rice varieties are longer basic vegetative period, more late maturing and higher ripening temperature. Barley and wheat crops could be shifted to northern coastal areas and apple production areas should be shifted to those areas under $13.5^{\circ}C$ in annual mean temperature at global warming. Ideotypes of crops under climate changes should have such ecological characteristics that are indispensable to accomplish the sustainable agriculture under increased $CO_{2}$ and temperature condition as the diversification of genetic resources from yield-oriented to biomass-oriented characteristics with the higher potentials of $CO_{2}$ absorption and Primary production. In addition, a heat-tolerance, a pest resistance, an environmental adaptability and a production stability should be also incorporated collectively into our integrated agroecosystem.

• 한국자원식물학회지
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• 제14권3호
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• pp.235-240
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• 2001

Polyphenol 화합물이 다량 함유된 식물종의 유연관계 분석이나 형질전환 유전자 확인 등을 위해 PCR을 이용할 경우 다량의 재료로부터 신속 간편하게 분리한 DNA를 이용할 수 있는 조건을 설정 하였다. 폴리페놀 함량이 높은 포도, 사과, 복분자와 같은 과수류에서 간편법에 의해 추출된 DNA를 이용한 PCR 반응액에 2%의 BLOTTO를 첨가함으로서 DNA의 재현적 증폭이 가능하였다. 간편 추출 DNA를 이용한 PCR에서 의 BLOTTO효과는 primer, 품종, 식물종에 관계없이 일반적으로 발현되었다. 상추의 형질전환 유전자 검색을 위한 PCR에서 도 BLOTTO 효과가 확인되었다. 따라서 PCR 반응액에 2% BLOTTO를 첨가하면 간편 법 에 의해 추출된 polyphenol 화합물 고함유 식물종의 DNA를 이용하여서도 PCR에 의한 유전배경 및 특정 유전자의 대량 신속 분석이 가능할 것이다.

• 디지털융복합연구
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• 제19권10호
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• pp.105-110
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• 2021

양돈 ICT 자동 급이기는 설정된 조건에 맞춰 사료 등을 자동 급이가 가능하다. 그러나, 설정 조건 자체는 사용자의 경험에 의존해야 하는 단점이 있다. 그렇기 때문에, 시행착오를 유발하고, 효율성이 떨어지는 문제가 발생하고 있다. 따라서, 데이터에 기반한 최적의 급이 설정 조건을 제시해 양돈 생산성을 향상시킬 수 있는 시스템 개발 및 서비스 모델 구현이 필요하다. 따라서, 본 논문에서는 기존의 급이 데이터, 사양관리 데이터 및 양돈 생산 관리 시스템 등의 성적분석 프로그램을 활용한 양돈 급이 관리 서비스 모델을 개발하였다. 이를 통해, 양돈 데이터 분석으로 효율적으로 활용할 수 있는 수요자 중심의 급이 관리 서비스 모델을 개발하였다. 또한, 지능화된 자동 급이 관리 서비스로 농가의 폐사율 감소 및 MSY 증가에 일조함으로써 양돈 농가의 생산성 향상과 이로 인해 양돈 농가의 소득 증대에 기여하는 서비스를 제공할 수 있다.