• 분석과학
• /
• 제31권4호
• /
• pp.171-178
• /
• 2018

Recently, for successful lactation, many breastfeeding mothers seek various products, including herbal medicine, dietary supplements, and prescribed medicines, to improve milk production. As demand for galactogogues grows, it is highly possible that pharmaceutical galactogogues may be adulterated with illegal products to maximize their efficacy. For continuous control and supervision of illegal products, we developed and validated a simple and sensitive LC-MS/MS method capable of simultaneously determining five galactogogues. Chromatographic separation was conducted using an Agilent Poroshell $120SB-C_{18}$ column with a mobile phase consisting of 20 mM ammonium formate (pH 5.4) and 100 % acetonitrile. The total run time was 13 min per analyte. The proposed method was performed according to the guidelines of the International Conference of Harmonization and it produced reliable results. This method showed high sensitivity and specificity, with a limit of detection (LOD) and limit of quantitation (LOQ) of 0.01-0.82 ng/mL and 0.02-2.45 ng/mL, respectively, for the solid- and liquid-type samples. Specificity was evaluated by analyzing matrix-blank samples spiked with the target compounds at LOQ levels, which provided a good separation of all peaks without interference. Additionally, the repeatability and intermediate precision were typically <15 %, whereas the recovery was 80-120 % of the values obtained using blank samples. Thus, we concluded that this method could be used for the identification and quantification of galactogogues in food or herbal products.

• Clinical and Experimental Pediatrics
• /
• 제52권8호
• /
• pp.888-892
• /
• 2009

목 적 : 신생아실에서 만삭아에 준한 경과 관찰 후 퇴원한 후기 조산아들의 재입원과 관련된 위험 요인과 재입원 원인을 알고자 연구를 계획하였다. 방 법 : 2003년 1월부터 2008년 12월까지 일신 기독 병원 신생아실에서 만삭아에 준한 경과 관찰 후 퇴원한 후기 조산아들 중 생후 28일 이내에 재입원하였던 135명의 신생아들의 의무 기록지를 후향적으로 조사하였다. 재입원의 위험 요인을 알고자 대조군과 비교 분석하였다. 결 과 : 재입원과 관련된 위험 요인 연구에서 재태주수, 출생 체중, 성별, 분만 방법, 산모의 나이, 교육 정도, 결혼 여부, 진통과 분만 과정상의 합병증은 관련이 없었고, 모유 수유(71.9% vs 44.4%), 짧은 신생아실 경과 관찰 기간($3.3{\pm}1.6$일 vs $4.1{\pm}2.0$일), 초산모(60.0% vs 45.3%)와 임신 합병증이 있었던 경우(31.9% vs 18.8%) 통계적으로 유의하게 재입원율이 높았다. 재입원 시점은 출생 후 평균 $6.2{\pm}3.6$일로, 출생 5-6일 사이에 재입원하는 경우가 40.7%로 가장 많았다. 재입원 시 83.7%가 황달을 주소로 입원하여 가장 흔한 원인이었고, 자연 분만(43.4% vs 1.8%), 산모의 나이가 적은 경우($29.8{\pm}3.4$세 vs $32.1{\pm}4.2$세), 임신과 관련된 합병증 동반이 적은 경우(28.3% vs 50%)가 황달로 인한 재입원과 관련 있었다. 결론 : 후기 조산아들의 재입원과 관련된 위험 요인은 모유 수유, 짧은 신생아실 경과 관찰 기간, 초산모와 임신 합병증이 있었던 경우였고, 재입원 시점은 평균 $6.2{\pm}3.6$일, 가장 흔한 재입원 원인은 황달이었다.

• 간호행정학회지
• /
• 제6권3호
• /
• pp.357-374
• /
• 2000

The purpose of this study was to validate knowledge outcomes included Nursing Outcomes Classification(NOC) developed by Johnson and Maas at the University of Iowa. A sample of 71 nurse experts working in university affiliated hospitals participated in this study. They were asked to rate indicators that examplified the outcomes on a scale of 1(indicator is not all characteristic) to 5(indicator is very characteristic). A questionnaire with an adaptation of Fehring's methodology was used to establish the content validity of outcomes. The results were as follow: 1. All indicators were considered to be 'supporting' and no indicators were considered to be 'nonsupporting'. 2. 'Knowledge: Treatment Regimen' attained and OCV score of 0.816 and was the highest OCV score among outcomes. 3. 'Knowledge: Energy Conservation' attained an OCV score of 0.748 and was the lowest OCV score among abuse outcomes. 4. 'Knowledge: Breastfeeding' attained an OCV score of 0.790 and was the highest indicator was 'description of benefits of breastfeeding'. 5. 'Knowledge: Child Safety' attained an OCV score of 0.778 and was the highest indicator was 'demonstration of first aids techniques'. 6. 'Knowledge: Diet' attained an OCV score of 0.779 and was the highest indicator was 'performance of self-monitoring activities'. 7. 'Knowledge: Disease Process' attained an OCV score of 0.815 and was the highest indicator was 'description of signs and symptoms'. 8. 'Knowledge: Health Behaviors' attained an OCV score of 0.800 and was the highest indicator was 'description of safe use of prescription drugs'. 9. 'Knowledge: Health Resources' attained an OCV score of 0.794 and was the highest indicator was 'description of need for follow-up care'. 10. 'Knowledge: Infection Control' attained an OCV score of 0.793 and was the highest indicator was 'description of signs and symptoms'. 11. 'Knowledge: Medication' attained an OCV score of 0.789 and was the highest indicator was 'description of correct administration of medication'. 12. 'Knowledge: Personal Safety' attained an OCV score of 0.804 and was the highest indicator was 'description of measures to reduce risk of accidental injury'. 13. 'Knowledge: Prescribed Activity' attained an OCV score of 0.810 and was the highest indicator was 'proper performance of exercise'. 14. 'Knowledge: Substance Use Control' attained an OCV score of 0.809 and was the highest indicator was 'description of signs of dependence during substance withdrawl'. 15. 'Knowledge: Treatment Procedure(s)' attained an OCV score of 0.795 and was the highest indicator was 'description of appropriate action for complications'. 16. 'Knowledge: Treatment Regimen' attained an OCV score of 0.816 and was the highest indicator was 'description of self-care responsibilities for emergency situations'. More outcomes need to be validated and outcomes sensitive to Korean culture need to be developed.

• 한국식품과학회지
• /
• 제53권6호
• /
• pp.775-784
• /
• 2021

Clostidium butyricum을 분리하기 위하여 국내 모유수유 신생아 분변으로부터 혐기성 균주를 선별하였고 버블을 생성하는 100개의 균을 확보하였다. 이중 Clostridium perfringens에 대한 항균력과 butyric acid의 생산이 가장 우수한 DIMO 52 균주를 선발하였고, 형태학적 특성, 생리 생화적 특성 및 16S rRNA 유전자 분석을 통하여 C. butyricum으로 동정되어 C. butyricum DIMO 52로 명명하였다. 성장률, butyric acid 생산 및 pH 변화를 배양 36시간 동안 모니터링하였다. 배양 24시간 후 DIMO 52 균주의 최대 성장에 도달하였고, butyric acid 최대 농도는 대략 34.73±4.27 mM이었으며, pH는 7.2에서 2.5로 변경되었다. DIMO 52 균주는 낮은 pH와 oxgall에 높은 저항성이 있다. pH 2에서 2시간 동안 접종의 약 67.5%의 유의성 있는 생존율을 보였다(p<0.05). 그리고, 0.3% oxgall이 함유된 RCM 액체배지에서 24시간 동안 접종의 약 64.9%의 유의성 있는 생존율을 보였다(p<0.05). 또한, DIMO 52은 Escherichia coli KCTC 2441와 Salmonella Typhimurium KCTC 1925에 대해 억제효과를 나타냈다. 두 균주에 대한 항균효과는 아마도 butyric acid에 의한 낮은 pH 때문인 것으로 보였다. 5×10³ CFU/mL 생균수 까지는 RAW264.7 세포에 세포독성이 없는 것으로 관찰되었고, NO 생성을 억제할 수 있는 최저 균수를 확인한 결과 약 1×10³ CFU/mL 생균수에서 LPS만 처리한 군 대비 약 33%의 NO 생성을 억제하는 것으로 분석되었다(p<0.01). 이 결과는 C. butyricum DIMO 52이 NO radical 소거 및 항염증 활성을 가지고 있음을 시사한다. 결론적으로, 본 연구에서 분리된 C. butyricum DIMO 52의 프로바이오틱스 특성을 확인하였다.

• Clinical and Experimental Reproductive Medicine
• /
• 제49권1호
• /
• pp.2-8
• /
• 2022

Humanity is in the midst of the coronavirus disease 2019 (COVID-19) pandemic, and vaccines-including mRNA vaccines-have been developed at an unprecedented speed. It is necessary to develop guidelines for vaccination for people undergoing treatment with assisted reproductive technology (ART) and for pregnancy-related situations based on the extant laboratory and clinical data. COVID-19 vaccines do not appear to adversely affect gametes, embryos, or implantation; therefore, active vaccination is recommended for women or men who are preparing for ART. The use of intravenous immunoglobulin G (IVIG) for the treatment of immune-related infertility is unlikely to impact the effectiveness of the vaccines, so COVID-19 vaccines can be administered around ART cycles in which IVIG is scheduled. Pregnant women have been proven to be at risk of severe maternal and neonatal complications from COVID-19. It does not appear that COVID-19 vaccines harm pregnant women or fetuses; instead, they have been observed to deliver antibodies against severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) to the fetus. Accordingly, it is recommended that pregnant women receive COVID-19 vaccination. There is no rationale for adverse effects, or clinical cases of adverse reactions, in mothers or neonates after COVID-19 vaccination in lactating women. Instead, antibodies to SARS-CoV-2 can be delivered through breast milk. Therefore, breastfeeding mothers should consider vaccination. In summary, active administration of COVID-19 vaccines will help ensure the safe implementation of ART, pregnancy, and breastfeeding.

• International Journal of Advanced Culture Technology
• /
• 제8권1호
• /
• pp.1-12
• /
• 2020

Pierre Robin syndrome is characterized by micrognathia, glossoptosis, and cleft palate. Infants with Pierre Robin syndrome causes feeding difficulty, upper airway obstruction, and other symptoms. This study aims to examine the effects of applying dysphagia treatment to infants with Pierre Robin syndrome. The study participant was an infant who was born four weeks premature and referred for dysphagia treatment approximately 100 days after birth. At the initial assessment, the infant showed oral sensory sensitivity, a high level of facial and masticatory muscle tension, and a low stability of the chin and cheeks with almost no normal "sucking-swallowing-breathing" pattern. We set the baseline period and intervention period using the AB design. During the baseline period, non-nutritive sucking training using a rubber nipple was conducted without implementing an oral stimulation intervention. During the intervention period, non-nutritive sucking training and an oral stimulation intervention were performed. After the intervention period, the infant's daily oral intake and oral intake per time significantly increased compared to that during the baseline period. We observed that the oral intake time of the infant decreased during the intervention period compared to that in the baseline period, which indicated an improvement in control over the chin, tongue, and lip movements, a change in muscular tension, and stabilization of the "sucking-swallowing-breathing" pattern. We provided dysphagia treatment before breastfeeding, it was positive effects such as normal development of the infant, transition from tube feeding to bottle feeding, and enhancement of overall oral motor function.

• Child Health Nursing Research
• /
• 제11권2호
• /
• pp.189-195
• /
• 2005

Purpose: The purpose of this study was to identify the characteristics and the related factors of attachment- oriented caretaking behavior in mothers who are breast-feeding their infants. Method: The subjects were 155 mothers who participated in a healthy breast-feeding contestin the Busan area. The data were collected from September 24, 2003, and were analyzed using SPSS WIN 10.0. Results: The mothers were highly affirmative in their attachment-oriented caretaking behaviors (total mean 2.59±.502 of a possible score of 3). Among the attachment-oriented caretaking behaviors (10 items), the scores for 6 items were higher than the average score and 4 items were lower than the average score. There were significant differences in the attachment-oriented caretaking behaviors according to level of education (p＜0.05), and planned duration of breast-feeding (p＜0.001). Mothers with university education who planned to breast-feed as long as the baby wanted had higher scores. Conclusions: The above results suggest that nursing interventions which are individualized and practical are needed to encourage the attachment-oriented caretaking behaviors essential to breast-feeding mothers.

• 여성건강간호학회지
• /
• 제2권1호
• /
• pp.68-87
• /
• 1996

The purpose of this study was to investigate the effects of breast-feeding education program on the promotion of mothers's feeding compliance during the 4 months after childbirth. A nonequivalent control group posttest design was used. Eighty-nine pregnant women at their 32 more weeks of gestation receiving antenatal care at the Chonnam University Hospital Obstetrics Clinic were assigned to the experimental group. Control group was ninety-nine pregnant women over 32wks of gestation receiving antenatal care at the Kwangju Christianity Hospital Obstetric Clinic during the same period. Breast-feeding education program was introduced to the experimental group from antepartal visit to 4 months after childbirth. Data were collected primarily via telephone interview on the 7th day, the end of 1 month and the 4 months after childbirth respectively. The results showed that 1) the frequency of breast-feeding continuation promoting behaviors was higher in the experimental group than in the control group. 2) The rates of mothers' compliance during the 4 months after childbirth in the experimental group demonstrated a significant difference ; much higher rate of mothers' compliance than control group. Conclusively, the breast-feeding education program increase the rate of mothers' compliance and duration of breast-feeding.

• 대한한방소아과학회지
• /
• 제26권1호
• /
• pp.70-78
• /
• 2012

Objectives Recently, vitamin D supplements to breast-fed infants are being encouraged in Korea, and other countries. However, the reliability and validity assessment of supplements is insufficient. Therefore, this study suggests new Korean guidelines for vitamin D supplements of breast-fed infant. Methods The roles of Vitamin D for bone metabolism in children and the correlation between breast milk and Vitamin D were examined throughout domestic and international literature review. In addition, the efficacy and safety of vitamin D supplements were reviewed. Results Preventive effects of rickets by vitamin D supplementation remain unclear. Furthermore, concerns about the safety of vitamin D supplements intake have been raised. Korean guideline suggests breast-fed infants can get vitamin D through the skin safely, but maternal intake of vitamins through sunbath and diet is more effective and safe. Conclusions Limiting sunlight excessively and applying foreign countries' medical guideline for vitamin D supplements are not valid to apply as a domestic guideline for Korean breast-fed infants without considering ethnic and cultural characteristics.

• Journal of Community Nutrition
• /
• 제6권2호
• /
• pp.78-85
• /
• 2004

Children are the most nutrition sensitive sub-group of a population. The nutritional status of children should be especially emphasized at all levels. This study was performed to investigate the current status, trend, and influencing factors to malnutrition of infants and children in China. The study was mainly based on the China Health and Nutrition Survey which is a longitudinal study conducted in 8 provinces and the data on growth of children under 7 years of age in 9 cities in China. The result of this study showed that one fifth of the children under 5 years of age are still suffering from stunted growth and one tenth suffering from underweight. The nutrition intervention on children under 2 years of age, especially on those under 18 months should be emphasized. Better supplementary food can improve the nutritional status to decrease the prevalence of stunted children. Therefore, the development of supplementary food should be the priority and should be emphasized with breastfeeding.