Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
권호 표지
DOI QR코드

DOI QR Code

Simultaneous determination of illegal galactagogue adulterants in supplement diets by LC-MS/MS

  • Lee, Ji Hyun (Division of Advanced Analysis, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex) ;
  • Cho, So-Hyun (Division of Advanced Analysis, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex) ;
  • Park, Han Na (Division of Advanced Analysis, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex) ;
  • Park, Hyoung Joon (Division of Advanced Analysis, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex) ;
  • Kim, Nam Sook (Division of Advanced Analysis, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex) ;
  • Park, Sung Kwan (Division of Advanced Analysis, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex) ;
  • Kang, Hoil (Division of Advanced Analysis, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex)
  • Received : 2018.07.05
  • Accepted : 2018.08.11
  • Published : 2018.08.25
Download PDF
⟨ Previous Next ⟩

Abstract

Recently, for successful lactation, many breastfeeding mothers seek various products, including herbal medicine, dietary supplements, and prescribed medicines, to improve milk production. As demand for galactogogues grows, it is highly possible that pharmaceutical galactogogues may be adulterated with illegal products to maximize their efficacy. For continuous control and supervision of illegal products, we developed and validated a simple and sensitive LC-MS/MS method capable of simultaneously determining five galactogogues. Chromatographic separation was conducted using an Agilent Poroshell $120SB-C_{18}$ column with a mobile phase consisting of 20 mM ammonium formate (pH 5.4) and 100 % acetonitrile. The total run time was 13 min per analyte. The proposed method was performed according to the guidelines of the International Conference of Harmonization and it produced reliable results. This method showed high sensitivity and specificity, with a limit of detection (LOD) and limit of quantitation (LOQ) of 0.01-0.82 ng/mL and 0.02-2.45 ng/mL, respectively, for the solid- and liquid-type samples. Specificity was evaluated by analyzing matrix-blank samples spiked with the target compounds at LOQ levels, which provided a good separation of all peaks without interference. Additionally, the repeatability and intermediate precision were typically <15 %, whereas the recovery was 80-120 % of the values obtained using blank samples. Thus, we concluded that this method could be used for the identification and quantification of galactogogues in food or herbal products.

Keywords

References

  1. M. Wilinska, and E. Schleubner, Nutrafoods, 14, 119-125 (2015). https://doi.org/10.1007/s13749-015-0034-9
  2. F. Penagos Tabares, J. V. Bedoya Jaramillo, and Z. T. Ruiz-Cortez, Vet. Med. Int., 2014, 1-20 (2014).
  3. A. A. Zuppa, P. Sindico, C. Orchi, C. Carducci, V. Cardiello, and C. Ramagnoli, J. Pharm. Pharm. Sci., 13, 162-174 (2010). https://doi.org/10.18433/J3DS3R
  4. A. B. Forinash, A. M. Yancey, K. L. Barnes, and T. D. Myles, Ann. Pharmacother., 46, 1392-1404 (2012). https://doi.org/10.1345/aph.1R167
  5. E.W. Wan, K. Davey, M. Page-Sharp, P. E. Hartmann, K. Simmer, and K. F. Ilett, Br. J. Clin Pharmacol., 66, 283-289 (2008). https://doi.org/10.1111/j.1365-2125.2008.03207.x
  6. F. J. Nice, Child Obes. Nutr., 3, 129-132 (2011).
  7. C. M. Betzold, and J. Midwifery Women's Health, 49, 151-154 (2004). https://doi.org/10.1016/j.jmwh.2003.12.007
  8. A. Osadchy, M. E. Moretti, and G. Koren, Obstet. Gynecol. Int., 2012, 1-7 (2012).
  9. M. Mortel and S. D. Mehta, J. Hum. Lact., 29, 154-162 (2013). https://doi.org/10.1177/0890334413477243
  10. I. Gbadamosi and O. Okolosi, J. Appl. Biosci., 61, 4460-4469 (2013). https://doi.org/10.4314/jab.v61i0.85594
  11. M. P. Gabay, J. Hum. Lact., 18, 274-279 (2002). https://doi.org/10.1177/089033440201800311
  12. H. C. Sachs, Pediatr., 132, 796-809 (2013). https://doi.org/10.1542/peds.2013-1493
  13. J. H. Lee, N. S. Kim, K. M. Han, S. H. Kim, S. Cho, and W. S. Kim, Food Addit. Contam. Part A, 30, 1849-1857 (2013). https://doi.org/10.1080/19440049.2013.826878
  14. H. J. Kim, J. H. Lee, H. J. Park, S. H. Cho, S. Cho, and W. S. Kim, Food Addit. Contam. Part A, 31, 777-783 (2014). https://doi.org/10.1080/19440049.2014.888497
  15. S. H. Cho, H. J. Park, J. H. Lee, H. J. Kim, S. Cho, C. Y. Yoon, and W. S. Kim, Food Addit. Contam. Part A, 31, 1470-1475 (2014). https://doi.org/10.1080/19440049.2014.946100
  16. M. Kobylinska, K. Kobylinska, J. Chromatogr. B., 744, 207-212 (2000). https://doi.org/10.1016/S0378-4347(00)00245-0
  17. L. Curtin Whelan, M. Geary, M. Wharton, and P. Sweetman, J. Chromatogr. Sci., 53, 226-232 (2015). https://doi.org/10.1093/chromsci/bmu044
  18. H. Lamparczyk, A. Chmielewska, L. Konieczna, A. Plenis, and P. K. Zarzycki, J. Biomed. Chromatogr., 15, 513-517 (2001). https://doi.org/10.1002/bmc.101
  19. L.Vlase, A. Leucuta, D. Farcau, and M. Nanulescu, J. Biopharm. Drug Dispos., 27, 285-289 (2006). https://doi.org/10.1002/bdd.510
  20. V. Michaud, C. Simard, and J. Turgeon, J. Chromatogr. B, 852, 611-616 (2007). https://doi.org/10.1016/j.jchromb.2007.02.044
  21. K. W. Riggs, A. Szeitz, D. W. Rurak, A. E. Mutlib, F. S. Abbott, and J. E. Axelson, J. Chromatogr. B, 660, 315-325 (1994). https://doi.org/10.1016/0378-4347(94)00314-9
  22. E. Saar, D. Gerostamoulos, and O. H. Drummer, J. Beyer, Anal. Bioanal. Chem., 393, 727-734 (2009). https://doi.org/10.1007/s00216-008-2498-6
  23. E. Saar, D. Gerostamoulos, O. H. Drummer, and J. Beyer, J. Mass Spectrom., 45, 915-925 (2010). https://doi.org/10.1002/jms.1783
  24. M. Yan, H. D. Li, B. M. Chen, X. L. Liu, and Y. G. Zhu, J. Chromatogr. B., 878, 883-887 (2010). https://doi.org/10.1016/j.jchromb.2010.02.006
  25. A. Bose, U. Bhaumik, A. Ghosh, B. Chatterjee, U. S. Chakrabarty, A. K. Sarkar, and T. K. Pal, Chromatogr., 69, 1233-1241 (2009). https://doi.org/10.1365/s10337-009-1032-0
  26. M. J. Smit, F. C. W. Sutherland, H. K. L. Hundt, K. J. Swart, A. F. Hundt, and J. Els, J. Chromatogr. A, 949, 65-70 (2002). https://doi.org/10.1016/S0021-9673(01)01553-9
  27. T. Kubo, K. Kuroda, Y. Tominaga, T. Naito, K. Sueyoshi, K. Hosoya, and K. Otsuka, J. Pharm. Biomed. Anal., 89, 111-117 (2014). https://doi.org/10.1016/j.jpba.2013.10.040