• Title/Summary/Keyword: brassinolide

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Determination of Brassinolide by HPLC equipped with Fluoresence Detector in Rice(Oriza sativa L.) (HPLC 형광분석법을 통한 벼에서 Brassinolide의 검정)

  • Kim, In-Seon;Lee, Kang-Bong;Suh, Yong-Tack;Morgan, E.D.;Shim, Jae-Han
    • Applied Biological Chemistry
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    • v.39 no.1
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    • pp.84-88
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    • 1996
  • To determine brassinolide in rice(Oriza sativa L.) using HPLC equipped with fluoresence detector, a highly sensitive fluorescence reagent. 1-cyanoisoindole-2-m-phenylboronic acid, was synthesized from the reaction of o-phthaldehyde, m-phenylboronic acid and KCN, then was reacted with brassinolide. The formation ratio of brassinolide boronate exhibited 90% up at the ratio of $20\;:\;1({\mu}g/{\mu}g)$ of 1-cyanoisoindole-2-m-phenylboronic acid and brassinolide respectively. The detection limit of brassinolide boronate with fluoresence detector was 0.16 ng. Brassinolide was detected in heading stage(biomass : 10 g) and panicle formation stage(biomass : 100 g) of the rice(Oryza sativa L.) with quantity of $0.8\;{\mu}g\;and\;0.2\;{\mu}g$respectively. However, brassinolide was not detected in blooming and elongation stage.

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Brassinosteroids Accelerate the Rate of Cell Division in Isolated Petal Protoplasts of Petunia hybrida

  • Oh, Man-Ho
    • Journal of Plant Biotechnology
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    • v.5 no.1
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    • pp.69-77
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    • 2003
  • Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been extensively studied. The effect of brassinolide on the kinetics and final division frequencies of regenerating petal protoplasts of Petunia hybrida Vilm v. Comanche was examined. Under optimal auxin and cytokinin conditions, 10-100 nM brassinolide not only reduced the time of first cell division by 4.5 days but also altered the final division frequencies after 10 days of culture. One micromolar brassinolide showed the same acceleration of first cell division but inhibited the final division frequency by approximately 9%. Under sub-optimal auxin conditions, 10-100 nM brassinolide accelerated the first cell division, but no significant increase in the 8-10 days final division frequencies. Isolated protoplasts may provide a useful model system for the investigation of the molecular mechanisms of brassinosteroid action on cell division and proliferation in higher plants.

Brassinosteroids Accelerate the Rate of Cell Division in Isolated Petal Protoplasts of Petunia hybrida

  • Oh, Man-Ho
    • Journal of Plant Biotechnology
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    • v.5 no.1
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    • pp.63-67
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    • 2003
  • Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been extensively studied. The effect of brassinolide on the kinetics and final division frequencies of regenerating petal protoplasts of Petunia hybrida Vilm v. Comanche was examined. Under optimal auxin and cytokinin conditions, 10-100 nM brassinolide not only reduced the time of first cell division by 4.5 days but also altered the final division frequencies after 10 days of culture. One micromolar brassinolide showed the same acceleration of first cell division but inhibited the final division frequency by approximately 9%. Under sub-optimal auxin conditions, 10-100 nM brassinolide accelerated the first cell division, but no significant increase in the 8-10 days final division frequencies. Isolated protoplasts may provide a useful model system for the investigation of the molecular mechanisms of brassinosteroid action on cell division and proliferation in higher plants.

Brassinosteroid substances in immature Cassia tora seeds (결명자의 brassinosteroid 활성물질)

  • Park, Keun-Hyung;Kim, Seon-Jae;Hyun, Kyu-Hawn
    • Applied Biological Chemistry
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    • v.36 no.2
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    • pp.99-104
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    • 1993
  • In order to explore the brassinosteroid-active component in Cassia tora, methanol extract of immature seeds was purified by sequences of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography, charcoal adsorption chromatography and Bondesil chromatography. The activity of brassinosteroid was monitored by the rice inclination test and its presence could be confirmed in each purification step. The purified active components were separated by silica gel adsorption chromatography. Brassinosteroid substances in separated active fractions were identified as teasterone, castasterone, brassinolide by TLC and HPLC. Our work is probably the first report of endogenous brassinosteroid in Cassia tora. The content of brassinosteroid in Cassia tora as converted into brassinolide was $3.5{\sim}5.5\;ng/g$ fresh weight. The order of brassinosteroid contents was toward to be teasterone, castasterone, brassinolide.

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Bioassays of Plant Hormones and Plant Growth Regulating Substances II. Abscisic Acid and Brassinolide (식물홀몬 및 생장조절물질의 생물검정기술 II. Abscisic Acid 및 Brassinolide)

  • 최충돈
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.s01
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    • pp.16-25
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    • 1989
  • A bioassay is a test system using a living organism (in whole or in part) to determine the presence or relative potency of chemical substances. The development and uses of bioassay are intimately linked to the discovery and characterization of the major classes of plant hormones. An application of this relationship is helpful for understanding the concept of plant hormones as well as the use of bioassay. And plant bioassay have been development and employed not only for the discovery and characterization of the biological activity of plant growth regulators but also have served several important secondary roles. The ideal bioassay should possess the characteristic of high specificity. great sensitivity. short response time, low cost and ease of obtaining plant material. acceptable ease of manipulation, and minimal space and equipment requirements.

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Occurrence of Castasterone, Brassinolide and Methyl 4-Chloroindole-3-acetate in Immature Vicia faba Seeds (잠두(Vicia faba L)가 생산하는 Brassinosteroid 활성물질)

  • Park, Keun-Hyung
    • Applied Biological Chemistry
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    • v.31 no.1
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    • pp.106-113
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    • 1988
  • The rice lamina inclination test indicated the presence of brassinosteroid-like active substances in immature Vicia faba seeds. Two of these were identified as castasterone and brassinolide by GC/MS and GC/SIM, respectively. Another active principle was identified as methyl 4-chloroindole-3-acetate by GC/MS and HPLC.

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