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Promotion of excisional wound repair by a menstrual blood-derived stem cell-seeded decellularized human amniotic membrane

  • Farzamfar, Saeed;Salehi, Majid;Ehterami, Arian;Naseri-Nosar, Mahdi;Vaez, Ahmad;Zarnani, Amir Hassan;Sahrapeyma, Hamed;Shokri, Mohammad-Reza;Aleahmad, Mehdi
    • Biomedical Engineering Letters
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    • v.8 no.4
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    • pp.393-398
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    • 2018
  • This is the first study demonstrating the efficacy of menstrual blood-derived stem cell (MenSC) transplantation via decellularized human amniotic membrane (DAM), for the promotion of skin excisional wound repair. The DAM was seeded with MenSCs at the density of $3{\times}10^4cells/cm^2$ and implanted onto a rat's $1.50{\times}1.50cm^2$ full-thickness excisional wound defect. The results of wound closure and histopathological examinations demonstrated that the MenSC-seeded DAM could significantly improve the wound healing compared with DAM-treatment. All in all, our data indicated that the MenSCs can be a potential source for cell-based therapies to regenerate skin injuries.

Imaging of Facial Nerve With 3D-DESS-WE-MRI Before Parotidectomy: Impact on Surgical Outcomes

  • Han-Sin Jeong;Yikyung Kim;Hyung-Jin Kim;Hak Jung, Kim;Eun-hye Kim;Sook-young Woo;Man Ki Chung;Young-Ik Son
    • Korean Journal of Radiology
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    • v.24 no.9
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    • pp.860-870
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    • 2023
  • Objective: The intra-parotid facial nerve (FN) can be visualized using three-dimensional double-echo steady-state water-excitation sequence magnetic resonance imaging (3D-DESS-WE-MRI). However, the clinical impact of FN imaging using 3D-DESS-WE-MRI before parotidectomy has not yet been explored. We compared the clinical outcomes of parotidectomy in patients with and without preoperative 3D-DESS-WE-MRI. Materials and Methods: This prospective, non-randomized, single-institution study included 296 adult patients who underwent parotidectomy for parotid tumors, excluding superficial and mobile tumors. Preoperative evaluation with 3D-DESS-WE-MRI was performed in 122 patients, and not performed in 174 patients. FN visibility and tumor location relative to FN on 3D-DESS-WE-MRI were evaluated in 120 patients. Rates of FN palsy (FNP) and operation times were compared between patients with and without 3D-DESS-WE-MRI; propensity score matching (PSM) and inverse probability of treatment weighting (IPTW) were used to adjust for surgical and tumor factors. Results: The main trunk, temporofacial branch, and cervicofacial branch of the intra-parotid FN were identified using 3D-DESS-WE-MRI in approximately 97.5% (117/120), 44.2% (53/120), and 25.0% (30/120) of cases, respectively. The tumor location relative to FN, as assessed on magnetic resonance imaging, concurred with surgical findings in 90.8% (109/120) of cases. Rates of temporary and permanent FNP did not vary between patients with and without 3D-DESS-WE-MRI according to PSM (odds ratio, 2.29 [95% confidence interval {CI} 0.64-8.25] and 2.02 [95% CI: 0.32-12.90], respectively) and IPTW (odds ratio, 1.76 [95% CI: 0.19-16.75] and 1.94 [95% CI: 0.20-18.49], respectively). Conversely, operation time for surgical identification of FN was significantly shorter with 3D-DESS-WE-MRI (median, 25 vs. 35 min for PSM and 25 vs. 30 min for IPTW, P < 0.001). Conclusion: Preoperative FN imaging with 3D-DESS-WE-MRI facilitated anatomical identification of FN and its relationship to the tumor during parotidectomy. This modality reduced operation time for FN identification, but did not significantly affect postoperative FNP rates.

A Study on Smoking and Relevant Factors among High School Students in Seoul Province (서울시내 일부 고등학생의 흡연 관련요인 분석)

  • Ahn, Byoung Ho;Park, Keeho;Kye, Su Yeon
    • The Journal of Korean Society for School & Community Health Education
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    • v.13 no.3
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    • pp.57-71
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    • 2012
  • Objectives: This study examines the situation regarding smoking among high school students in Seoul Province and identifies smoking-related factors. Methods: The study sample consisted of 1,000 high school second-grade students from 6 high schools in Seoul. The PRECEDE model was used to assess the students' needs. We carried out an educational diagnosis on attitudes, outcome expectations and social norms. Date were collected from June. 7 to 21, 2011, and were analyzed using SPSS-15.0 according to the study objectives. Results: Of 906 respondents, 9.4% had experiences to smoking: 12.7% were male and 5.7% were female. Smoking-related factors from among general characteristics were statistically significant depending on the degree of gender($x^2$=14.515, p=.001), school performance($x^2$=40.289, p=.001) and friends 'smoking status($x^2$=88.615, p=.001). Factors concerning attitudes toward smoking were statistically significant depending on the students' perceptions as follows: 'Smoking is fun(t=-14.801, p=.000)', 'Smoking looks cool(t=-10.349, p=.000)', 'People who smoke have more friends(t=-11.295, p=.000)', 'Smoking helps me manage stress(t=-15.059, p=.000)' and 'Smoking is not harmful to the body if you exercise(t=-6.388, p=.000)'. Factors concerning outcome expectations were statistically significant depending on their perceptions as follows: 'Tobacco smells good(t=-8.939, p=.000)' and 'Smoking helps in weight management (t=-7.304, p=.000)'. Factors concerning social norms were statistically significant depending on the following perception: 'My friends will not like it if I smoke(t=4.605, p=.000)'. The following influence high school students attitudes toward smoking: School performance (OR=11.66, 95%CI=1.67~81.37; OR=18.27, 95%CI=2.58~129.24; OR=26.74, 95%CI=3.06~233.79), Friends smoking status(OR=80.05, 95%CI=6.94~922.77), 'Smoking is fun(OR=12.90, 95%CI=3.87~43.04; OR=63.41, 95%CI=10.66~377.09)', 'Smoking looks cool(OR=0.15, 95%CI=0.03~0.64)', 'Smoking is not harmful to the body if you exercise(OR=1.44, 95%CI=0.03~0.62)', 'When there is no work to do, smoking is a good way to pass the time(OR=21.68, 95%CI=4.27~109.90)', 'When you are angry, smoking calms you down.(OR=13.39, 95%CI=3.92~45.65; OR=8.69, 95%CI=1.67~45.13)' and 'My parents consider it important that I do not smoke(OR=10.05, 95%CI=1.00~100.43)'. Conclusions: The study suggests that effective ways to discourage of high school students from smoking are changing their attitudes toward smoking, reducing the motivation to smoke, and controlling the number of cigarettes. Therefore, aiming at preventive education. Schools must provide accurate information about the effects of smoking. Thus, health education should actively involve preventive education not only in schools but also at home, the societal and national levels. Cooperation between various sectors of society is required for this.

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Hybrid Technique to Correct Cerebral Malperfusion Following Repair of a Type A Aortic Dissection

  • Kim, Seon Hee;Song, Seunghwan;Kim, Sang-Pil;Lee, Jonggeun;Lee, Han Cheol;Kim, Eun Soo
    • Journal of Chest Surgery
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    • v.47 no.2
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    • pp.163-166
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    • 2014
  • A 49-year-old man with drowsy mentality was diagnosed with acute type A aortic dissection; he underwent an emergency operation. When selective antegrade cerebral perfusion was initiated, the right regional cerebral oxygen saturation ($rSO_2$) decreased as compared to the left one. Adequate blood flow was perfused through the branch of the artificial graft, after distal anastomosis, but the right $rSO_2$ did not recover. Angiography revealed another intimal tear on the right common carotid artery. A stent was then inserted. The right $rSO_2$ promptly increased to the same level as that of the left one. The patient was discharged without any neurologic complications.

Effect of the Feeding of Brown Rice Flake with Soy Milk or Cow's Milk to School Children in Remote Village (비급식 농촌 국민학교 아동의 현미 플레이크와 두유 또는 우유 간식 급여의 영양효과에 관한 연구)

  • 박재창
    • Journal of Nutrition and Health
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    • v.25 no.1
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    • pp.42-55
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    • 1992
  • To evaluate the effect of the feeding program of brown rice flake with soy milk or cow's milk to school children in rural areas. The survey was conducted twice before and after practice of feeding in March and July of 1990. The survey covered a total of 87 children aged from 7 to 12 in Ypsuk elementary school located in remote village of Sangju county. Kyung Buk province and the branch school of Songmyun elementary school located in remote village of Goisan county. Chung Buk province. Family environment dietary intake anthropometric data and biochemical tests were examined. The results are summerized as follows: The mean balance and food diversity at lunch were significantly lower than those at breakfast and dinner. Mean daily energy and nutrient intakes were below the Korean RDA's except for intakes of niacin vitamin A and ascorbic acid Especially intake of calcium was not reached to 50% of Korean RDA's. Most of anthropometric data of subjects surveyed showed slightly higher results in summer than those in spring except for weight for height and sitting height for height. The percentage of anemic according to hematocrit criterion was not reduced during this period. however urinary urea nitrogen/creatinine ratio was improved. The conbined feeding program of brown rice flake with soy milk or cow's milk was not sufficient to support the children's growth. So the survey recommends the support of goberment to the school lunch program in the remote village.

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Irradiation-resistant Properties of Structurally Controlled Molybdenum Alloys Through a Multi-step Internal Nitriding

  • Nakahara, Takayuki;Okamoto, Yoshihisa;Nagae, Masahiro;Yoshio, Tetsuo;Kurishita, Hiroaki;Takada, Jun;Hiraoka, Yutaka;Takida, Tomohiro
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09b
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    • pp.1161-1162
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    • 2006
  • In order to overcome the recrystallization embrittlement and irradiation embrittlement of Mo, which are major problems for its fusion applications, internally nitrided Mo alloys were prepared by a novel multi-step internal nitriding. Neutron irradiation was performed in the Japan Material Testing Reactor (JMTR). After irradiation, nitrided Mo alloys exhibited $\iota$ ower ductile-brittle transition temperature than irradiated TZM. These results suggested that multi-step internal nitriding was effective to the improvement in the embrittlement by irradiation. Transmission electron microscope observation revealed that TiN particles precipitated by nitriding acted as a sink for irradiation-induced defects.

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On DC-Side Impedance Frequency Characteristics Analysis and DC Voltage Ripple Prediction under Unbalanced Conditions for MMC-HVDC System Based on Maximum Modulation Index

  • Liu, Yiqi;Chen, Qichao;Li, Ningning;Xie, Bing;Wang, Jianze;Ji, Yanchao
    • Journal of Power Electronics
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    • v.16 no.1
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    • pp.319-328
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    • 2016
  • In this study, we first briefly introduce the effect of circulating current control on the modulation signal of a modular multilevel converter (MMC). The maximum modulation index is also theoretically derived. According to the optimal modulation index analysis and the model in the continuous domain, different DC-side output impedance equivalent models of MMC with/without compensating component are derived. The DC-side impedance of MMC inverter station can be regarded as a series xR + yL + zC branch in both cases. The compensating component of the maximum modulation index is also related to the DC equivalent impedance with circulating current control. The frequency characteristic of impedance for MMC, which is observed from its DC side, is analyzed. Finally, this study investigates the prediction of the DC voltage ripple transfer between two-terminal MMC high-voltage direct current systems under unbalanced conditions. The rationality and accuracy of the impedance model are verified through MATLAB/Simulink simulations and experimental results.

A prediction model of low back pain risk: a population based cohort study in Korea

  • Mukasa, David;Sung, Joohon
    • The Korean Journal of Pain
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    • v.33 no.2
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    • pp.153-165
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    • 2020
  • Background: Well-validated risk prediction models help to identify individuals at high risk of diseases and suggest preventive measures. A recent systematic review reported lack of validated prediction models for low back pain (LBP). We aimed to develop prediction models to estimate the 8-year risk of developing LBP and its recurrence. Methods: A population based prospective cohort study using data from 435,968 participants in the National Health Insurance Service-National Sample Cohort enrolled from 2002 to 2010. We used Cox proportional hazards models. Results: During median follow-up period of 8.4 years, there were 143,396 (32.9%) first onset LBP cases. The prediction model of first onset consisted of age, sex, income grade, alcohol consumption, physical exercise, body mass index (BMI), total cholesterol, blood pressure, and medical history of diseases. The model of 5-year recurrence risk was comprised of age, sex, income grade, BMI, length of prescription, and medical history of diseases. The Harrell's C-statistic was 0.812 (95% confidence interval [CI], 0.804-0.820) and 0.916 (95% CI, 0.907-0.924) in validation cohorts of LBP onset and recurrence models, respectively. Age, disc degeneration, and sex conferred the highest risk points for onset, whereas age, spondylolisthesis, and disc degeneration conferred the highest risk for recurrence. Conclusions: LBP risk prediction models and simplified risk scores have been developed and validated using data from general medical practice. This study also offers an opportunity for external validation and updating of the models by incorporating other risk predictors in other settings, especially in this era of precision medicine.

Analysis of Organic Compounds in Ambient PM2.5 over Seoul using Thermal Desorption-comprehensive Two Dimensional Gas Chromatography-time of Flight Mass Spectrometry (TD-GCxGC-TOFMS) (Thermal Desorption-comprehensive Two Dimensional Gas Chromatography-time of Flight Mass Spectrometry (TD-GCxGC-TOFMS)을 이용한 서울 대기 중 PM2.5 유기성분 분석)

  • Lee, Ji-Yi;Lane, Douglas A.;Huh, Jong-Bae;Yi, Sung-Muk;Kim, Yong-Pyo
    • Journal of Korean Society for Atmospheric Environment
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    • v.25 no.5
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    • pp.420-431
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    • 2009
  • Characteristics and advantages of the thermal desorption-comprehensive two dimensional gas chromatography-time of flight mass spectrometry (TD-GCxGC-TOFMS) were discussed and the organic compound's analysis result was shown for the ambient $PM_{2.5}$ sample collected in Seoul, Korea. Over 10,000 individual organic compounds were separated from about $70{\mu}g$ of aerosols in a single procedure with no sample pre-treatment. Among them, around 300 compounds were identified and classified based on the mass fragmentation patterns and GCxGC retention times. Several aliphatic compounds groups such as alkanes, alkenes, cycloalkanes, alkanoic acids, and alkan-2-ones were identified as well as 72 PAH compounds including alkyl substituted compounds and 8 hopanes. In Seoul aerosol, numerous oxidized aromatic compounds including major components of secondary organic aerosols were observed. The inventory of organic compounds in $PM_{2.5}$ of Seoul, Korea suggested that organic aerosol were constituted by the compounds of primary source emission as well as the formation of secondary organic aerosols.

Analysis of PSK Coherent Carrier Signal Recovery Circuit Using Six-Port Phase Correlator (6-단자 위상 상관기를 이용한 PSK 반송파 신호 복원 회로 해석)

  • Kim, Young-Wan;Shin, Choo-Yeon
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.11
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    • pp.1281-1286
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    • 2008
  • The PSK carrier signal recovery circuit using a six-port phase correlator was analyzed and the circuit structure is proposed in this paper. The proposed carrier signal recovery circuit that is made of reflection element and six-port phase correlator, which is comprised of a power divider and three hybrid branch line couplers, give a simple structure and can be fabricated without no difficulty. The circuit recovers the carrier signal of BPSK and QPSK modulation signal. The proposed scheme can be utilized as a basis structure for high-mode PSK carrier signal recovery. By simulation results, the recovered signal by the proposed circuit shows a good carrier signal characteristic with CW signal of a constant phase($23.4^{\circ}$) and ${\pm}0.8^{\circ}$ phase error due to glitch conditions.