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Assessment of Root-Associated Paenibacillus polymyxa Groups on Growth Promotion and Induced Systemic Resistance in Pepper

  • Phi, Quyet-Tien;Park, Yu-Mi;Seul, Keyung-Jo;Ryu, Choong-Min;Park, Seung-Hwan;Kim, Jong-Guk;Ghim, Sa-Youl
    • Journal of Microbiology and Biotechnology
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    • v.20 no.12
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    • pp.1605-1613
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    • 2010
  • Twenty-nine P. polymyxa strains isolated from rhizospheres of various crops were clustered into five genotypic groups on the basis of BOX-PCR analysis. The characteristics of several plant growth-promoting factors among the isolates revealed the distinct attributes in each allocated group. Under gnotobiotic conditions, inoculation of pepper roots with P. polymyxa isolates significantly increased the biomass in 17 of total 29 treated plants with untreated plants. Experiments on induced systemic resistance (ISR) against bacterial spot pathogen Xanthomonas axonopodis pv. vesicatoria in pepper by P. polymyxa strains were conducted and only one isolate (KNUC265) was selected. Further studies into ISR mediation by the KNUC265 strain against the soft-rot pathogen Erwinia carotovora subsp. carotovora in tobacco demonstrated that the tobacco seedlings exposed to either bacterial volatiles or diffusible metabolites exhibited a reduction in disease severity. In conclusion, ISR and plant growth promotion triggered by P. polymyxa isolates were systemically investigated on pepper for the first time. The P. polymyxa KNUC265 strain, which elicited both ISR and plant growth promotion, could be potentially used in improving the yield of pepper and possibly of other crops.

Lipoteichoic Acid Suppresses Effector T Cells Induced by Staphylococcus aureus-Pulsed Dendritic Cells

  • Son, Young Min;Song, Ki-Duk;Park, Sung-Moo;Han, Seung Hyun;Yun, Cheol-Heui
    • Journal of Microbiology and Biotechnology
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    • v.23 no.7
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    • pp.1023-1030
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    • 2013
  • Lipoteichoic acid (LTA), uniquely expressed on gram-positive bacteria, is recognized by Toll-like receptor 2 (TLR2) on not only antigen-presenting cells but also activated T cells. Therefore, it is reasonable to assume that LTA is acting on T cells. However, little is known about the effect of LTA on T-cell regulation. In the present study, we investigated the immunomodulatory effects of LTA on $CD4^+$ T cells. Effector $CD4^+$ T cells, induced after co-culture with S. aureus-pulsed dendritic cells, produced high levels of interferon-${\gamma}$, CD25, CD69, and TLRs 2 and 4. When effector $CD4^+$ T cells were treated with LTA, the expressions of the membrane-bound form of transforming growth factor (TGF)-${\beta}$ and forkhead box P3 increased. Coincidently, the proliferation of effector $CD4^+$ T cells was declined after LTA treatment. When TGF-${\beta}$ signaling was blocked by the TGF-${\beta}$ receptor 1 kinase inhibitor, LTA failed to suppress the proliferation of effector $CD4^+$ T cells. Therefore, the present results suggest that LTA suppresses the activity of effector $CD4^+$ T cells by enhancing TGF-${\beta}$ production.

An Experimental Study on the Performance of Multi-Diameter Lateral for Riverbed Filtration (하상여과용 다직경 수평집수관의 효능에 대한 실험연구)

  • Bae, Gha-Ram;Kim, Seung-Hyun
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.5
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    • pp.359-364
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    • 2012
  • In order to prevent the decrease in well efficiencies due to friction in the axial flow in long, large-diameter laterals, a multi-diameter lateral was devised and tested through lab-scale sand-box experiments to assess its performance. In the experiment, three different production rates were applied over the multi-diameter and the three single-diameter laterals to obtain the hydraulic head distributions for each, which was used to assess the performance of the laterals. Results elucidated that the multi-diameter lateral reduced the material cost by more than a third, in comparison to the single-diameter lateral, while maintaining the production rate at higher than 93%, proving its superiority. Furthermore, results indicated that exit velocities exceeding 0.8 m/sec in horizontal wells tended to distort the hydraulic head distribution near the exit, providing evidence of its inefficiency.

The Study about Necessity of Production Managing Program in 3D Animation - Focus on Lighting Production Pipeline - (3D애니메이션에서 제작관리프로그램의 필요성에 관한 연구 - 라이팅 제작파이프라인 중심으로 -)

  • Lee Jung-Ho;Kim Hyun-Jo
    • The Journal of the Korea Contents Association
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    • v.6 no.5
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    • pp.66-75
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    • 2006
  • Korea animation industry has a face with many difficulties even if the worldwide animation industry is booming stage. The funding of korea animation industry has been getting decreased from the outside which are ordinary investors and government since 'Wonderful Days' showed on the screen in 2003 didn't has enough box-office profits. In this kinds of circumstance, Animation producers should check out the current production system very closely and restructure improvement factors resolutely. It is very important issue that the serious problems that have been repeated in the past like the absence of planning skill and saving time and money through the improvement of production system. Therefore, the purpose of this thesis is to check out the whole production process used in the current 30 animation industry and analyzes problems, show the necessity of the production management program that makes a given condition of the effective production system in 3d animation.

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THE EFFECT OF ADHESIVE CURING TIMING ON THE DIRECTION OF POLYMERIZATION SHRINKAGE OF COMPOSITE RESIN (상아질 접착제의 중합 시간 조절에 따른 복합레진의 중합 수축 방향의 변화)

  • 배지현;오명환;김창근;손호현;엄정문;조병훈;권혁춘
    • Restorative Dentistry and Endodontics
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    • v.26 no.4
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    • pp.316-325
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    • 2001
  • The purpose of this study was to evaluate the effect of adhesive curing timing on the direction of polymerization shrinkage of light-curing composite resin. In this study, the curing times of adhesive and composite resin were measured by differential scanning calorimeter(DSC). 28 extracted human molars were embedded in clear resin and box-type cavities were prepared. Based on DSC data, the experimental teeth were divided into 4 groups. Group 1: no bond; Group 2: late curing; Group 3: Intermediate curing; Group 4: Early curing. After treating with adhesive, the buccal cavities were filled with Z-100 hybrid composite resin and the lingual ones were filled with AEliteflo flowable composite resin. The depressions at the surface were measured by surface profilometer, then the specimens were embedded in clear resin and sectioned. Impressions were obtained and used to get epoxy resin replicas. The epoxy replicas were gold-coated and observed under SEM. Average Maximum Gap(AMG), Gap Proportion(GP), Average Marginal Index(AMI) were used to compare the shrinkage gap of each group. The results were statistically analyzed using the Kruskal-Wallis One Way ANOVA, Student-Newman-Keuls method. The results of this study were as follows. 1. Average Maximum Gap, Gap Proportion, Average Marginal Index and depression at the surface or Z-100 hybride composite resin were smaller than those of AEliteflo flowable composite resin(P<0.05). 2. When the bonding between composite resin and tooth structure was strong, the shrinkage gap was small, and depression at the surface was deep(P<0.05). 3. In the well-bonded group, light-curing composite resin shrank toward bonded cavity wall, not toward light source. The result suggested that the direction of polymerization shrinkage was affected by the quality of bonding in the dentin-resin interface. The strong was the bonding between composite resin and tooth structure, the smaller was the gap and the deeper was the depression at the surface. Then the flow to compensate the polymerization shrinkage proceeded from surface to bonded cavity wall.

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Elemental analysis of caries-affected root dentin and artificially demineralized dentin

  • Sung, Young-Hye;Son, Ho-Hyun;Yi, Keewook;Chang, Juhea
    • Restorative Dentistry and Endodontics
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    • v.41 no.4
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    • pp.255-261
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    • 2016
  • Objectives: This study aimed to analyze the mineral composition of naturally- and artificially-produced caries-affected root dentin and to determine the elemental incorporation of resin-modified glass ionomer (RMGI) into the demineralized dentin. Materials and Methods: Box-formed cavities were prepared on buccal and lingual root surfaces of sound human premolars (n = 15). One cavity was exposed to a microbial caries model using a strain of Streptococcus mutans. The other cavity was subjected to a chemical model under pH cycling. Premolars and molars with root surface caries were used as a natural caries model (n = 15). Outer caries lesion was removed using a carbide bur and a hand excavator under a dyeing technique and restored with RMGI (FujiII LC, GC Corp.). The weight percentages of calcium (Ca), phosphate (P), and strontium (Sr) and the widths of demineralized dentin were determined by electron probe microanalysis and compared among the groups using ANOVA and Tukey test (p < 0.05). Results: There was a pattern of demineralization in all models, as visualized with scanning electron microscopy. Artificial models induced greater losses of Ca and P and larger widths of demineralized dentin than did a natural caries model (p < 0.05). Sr was diffused into the demineralized dentin layer from RMGI. Conclusions: Both microbial and chemical caries models produced similar patterns of mineral composition on the caries-affected dentin. However, the artificial lesions had a relatively larger extent of demineralization than did the natural lesions. RMGI was incorporated into the superficial layer of the caries-affected dentin.

Heat Spreading Properties of CVD Diamond Coated Al Heat Sink (CVD 다이아몬드가 코팅된 알루미늄 방열판의 방열 특성)

  • Yoon, Min Young;Im, Jong Hwan;Kang, Chan Hyoung
    • Journal of Surface Science and Engineering
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    • v.48 no.6
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    • pp.297-302
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    • 2015
  • Nanocrystalline diamond(NCD) coated aluminium plates were prepared and applied as heat sinks for LED modules. NCD films were deposited on 1 mm thick Al plates for times of 2 - 10 h in a microwave plasma chemical vapor deposition reactor. Deposition parameters were the microwave power of 1.2 kW, the working pressure of 90 Torr, the $CH_4/Ar$ gas ratio of 2/200 sccm. In order to enhance diamond nucleation, DC bias voltage of -90 V was applied to the substrate during deposition without external heating. NCD film was identified by X-ray diffraction and Raman spectroscopy. The Al plates with about 300 nm thick NCD film were attached to LED modules and thermal analysis was carried out using Thermal Transient Tester (T3ster) in a still air box. Thermal resistance of the module with NCD/Al plate was 3.88 K/W while that with Al plate was 5.55 K/W. The smaller the thermal resistance, the better the heat emission. From structure function analysis, the differences between junction and ambient temperatures were $12.1^{\circ}C$ for NCD/Al plate and $15.5^{\circ}C$ for Al plate. The hot spot size of infrared images was larger on NCD/Al than Al plate for a given period of LED operation. In conclusion, NCD coated Al plate exhibited better thermal spreading performance than conventional Al heat sink.

Browning end Color Characteristics in Mushrooms (Agaricus bisporus) As Influenced by ionizing Energy (버섯의 갈변 및 색도에 대한 전리에너지의 영향)

  • Kwon, Joong-Ho;Byun, Myung-Woo;Cho, Han-Ok
    • Korean Journal of Food Science and Technology
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    • v.22 no.5
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    • pp.509-513
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    • 1990
  • Browning and color characteristics of stored mushrooms (Agaricus bisporus)following ionizing irradiation were investigated in connection with quality deterioration. The phenolic compounds of stored mushrooms showed a gradual decreasing tendency, while extractable browning pigments apparently rose from around 3days of storage under the conditions of $9{\pm}1^{\circ}C,\;80{\pm}7%$ RH and packaging with a corrugated paper box wrapped up in PE. ${\gamma}-irradiation$ at 2 to 3 kGy resulted in a significant reduction of their changes. Immediately after treatment, irradiated mushrooms were more discolored, i.e. a lower Hunter L value and higher Hunter a and b values than control. However, the subsequent storage for 15 days resulted in a preventive influence of ionizing energy on mushroom discoloration. This beneficial effect of ionizing energy was somewhat higher in the pilei than in the stipes of mushrooms and was found to increase lineally with increasing doses up to 3 kGy.

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Dynamic Centrifuge Tests for Evaluating the Earthquake Load of the Structure on Various Foundation Types (다양한 기초 형식에 따른 단자유도 구조물 지진하중 평가를 위한 동적 원심모형실험)

  • Ha, Jeong Gon;Jo, Seong Bae;Park, Heon Joon;Kim, Dong Kwan;Kim, Dong Soo
    • Journal of the Earthquake Engineering Society of Korea
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    • v.20 no.5
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    • pp.285-293
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    • 2016
  • Soil-foundation-structure interaction (SFSI) is one of the important issues in the seismic design for evaluating the exact behavior of the system. A seismic design of a structure can be more precise and economical, provided that the effect of SFSI is properly taken into account. In this study, a series of the dynamic centrifuge tests were performed to compare the seismic response of the single degree of freedom(SDOF) structure on the various types of the foundation. The shallow and pile foundations were made up of diverse mass and different conjunctive condition, respectively. The test specimen consisted of dry sand deposit, foundation, and SDOF structure in a centrifuge box. Several types of earthquake motions were sequentially applied to the test specimen from weak to strong intensity of them, which is known as a stage test. Results from the centrifuge tests showed that the seismic responses of the SDOF structure on the shallow foundation and disconnected pile foundation decreased by the foundation rocking. On the other hand, those on the connected pile foundation gradually increased with intensity of input motion. The allowable displacement of the foundation under the strong earthquake, the shallow and the disconnected pile foundation, have an advantage in dissipating the earthquake energy for the seismic design.

Driver Assistance System By the Image Based Behavior Pattern Recognition (영상기반 행동패턴 인식에 의한 운전자 보조시스템)

  • Kim, Sangwon;Kim, Jungkyu
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.12
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    • pp.123-129
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    • 2014
  • In accordance with the development of various convergence devices, cameras are being used in many types of the systems such as security system, driver assistance device and so on, and a lot of people are exposed to these system. Therefore the system should be able to recognize the human behavior and support some useful functions with the information that is obtained from detected human behavior. In this paper we use a machine learning approach based on 2D image and propose the human behavior pattern recognition methods. The proposed methods can provide valuable information to support some useful function to user based on the recognized human behavior. First proposed one is "phone call behavior" recognition. If a camera of the black box, which is focused on driver in a car, recognize phone call pose, it can give a warning to driver for safe driving. The second one is "looking ahead" recognition for driving safety where we propose the decision rule and method to decide whether the driver is looking ahead or not. This paper also shows usefulness of proposed recognition methods with some experiment results in real time.