• Molecules and Cells
• /
• v.24 no.3
• /
• pp.323-328
• /
• 2007

SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were up-regulated in the SOX15-NT2/D1 cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.

• Journal of Korean Society of Steel Construction
• /
• v.17 no.3 s.76
• /
• pp.357-363
• /
• 2005

There is a great need for high-strength steel especially for the high-rise steel building structure. High-strength steels, however, may have mechanical properties that are significantly different from those of the conventional steels. The application of high-strength steels to building structures should be reviewed as to whether the inelastic behavior equivalent to that of conventional steels can be attained or not. In this study, SM570TMC steel was tested to evaluate buckling strength under axial compressive force. The comparison tests for local buckling strength evaluation of box-type and H-shaped welded columns were performed with variable width-thickness ratios. As for the experimental check, the maximum strength of stub column was determined by local buckling as far as the limit of width-to-thickness ratio was satisfied with current design codes. Also, the strength of the stub column did not decrease suddenly by local buckling before maximum strength even when the ratio is not satisfied. The buckling strength of SM570TMC steel was higher than both ASD (Allowable Stress Design) and LRFD (Load and Resistance Factor Design) specifications.

• Genomics & Informatics
• /
• v.6 no.4
• /
• pp.231-234
• /
• 2008

Precise and reliable identification of CNV is still important to fully understand the effect of CNV on genetic diversity and background of complex diseases. SNP marker has been used frequently to detect CNVs, but the analysis of SNP chip data for identifying CNV has not been well established. We compared various normalization methods for CNV analysis and suggest optimal normalization procedure for reliable CNV call. Four normal Koreans and NA10851 HapMap male samples were genotyped using Affymetrix Genome-Wide Human SNP array 5.0. We evaluated the effect of median and quantile normalization to find the optimal normalization for CNV detection based on SNP array data. We also explored the effect of Robust Multichip Average (RMA) background correction for each normalization process. In total, the following 4 combinations of normalization were tried: 1) Median normalization without RMA background correction, 2) Quantile normalization without RMA background correction, 3) Median normalization with RMA background correction, and 4) Quantile normalization with RMA background correction. CNV was called using SW-ARRAY algorithm. We applied 4 different combinations of normalization and compared the effect using intensity ratio profile, box plot, and MA plot. When we applied median and quantile normalizations without RMA background correction, both methods showed similar normalization effect and the final CNV calls were also similar in terms of number and size. In both median and quantile normalizations, RMA backgroundcorrection resulted in widening the range of intensity ratio distribution, which may suggest that RMA background correction may help to detect more CNVs compared to no correction.

• Reproductive and Developmental Biology
• /
• v.32 no.4
• /
• pp.211-215
• /
• 2008

The HMG box containing protein (HBP) has a high mobility group domain and involved in the regulation of proliferation and differentiation of tissues. We screened HBP2 in glioblastoma using Suppression Subtractive Hybridization (SSH) and isolated human spermatogonial stem cell-like cells (hSSC-like cells) derived from patients of nonobstructive azoospermia (NOA). Expression of HBP2 was analyzed by RT-PCR in undifferentiated stem cells (human Embryonic Stem Cells, hSSC-like cells 2P) and spontaneous differentiated stem cells (hSSC-like cells 4P). It was overexpressed in hESC and hSSC-like cells 2P but not in hSSC-like cells 4P. Also, the expression level of HBP2 was downregulated in colon tumor tissues compared to normal tissues. Specifically in synchronized WI-38 cells, HBP2 was highly upregulated until the G1 phase of the cell cycle and gradually decreased during the S phase. Our results suggest that HBP2 was downregulated during the spontaneous differentiation of hSSC-like cells. HBP2 was differently expressed in colon tissues and was related to G1-progression in WI-38 cells. It may playa role in the maintenance of an undifferentiated hSSC-like cell state and transits from G1 to S in WI-38 cells. This research was important that it identified a biomarker for an undifferentiated state of hSSC-like cells and characterized its involvement to arrest during cell cycle in colon cancer.

• Korean System Dynamics Review
• /
• v.8 no.2
• /
• pp.51-82
• /
• 2007

The purpose of this paper is exploring changes in land use pattern when considering environmental carrying capacity. A sustainable development requires a society to define sustainability constraints, environmental carrying capacity. Environmental carrying capacity can be defined as a level of human activity a region can sustain at a desired level of quality of environment. This concept of environmental carrying capacity can be applied to land use to explore sustainable land use pattern. Since land use pattern can affect environment in an important way, exploring sustainable land use pattern within the limit of environmental carrying capacity can suggest useful implications for a sustainable regional management and planning. For this purpose, this paper built the environmental carrying capacity land use model and applied it to the Metropolitan Area, Korea. System dynamics modeling methods was used to build the model. The model developed in this paper consisted of 6sectors; population, housing, industry, land, environment, and traffic sector. The model limits its main focus on the NO2 level as an indicator of quality of environment in Metropolitan Area. Box model was translated into system dynamics model and combined to urban dynamics model to estimate NO2 level, the maximum number of population, industry structure, housing and maximum amount of land use for industrial, housing, and green space that can sustain desirable NO2 level. Metropolitan area was divided into 16 areas and the model was applied to each area. Since NO2 is flowing in and out from each area, model was built to allow this transboundering nature of air pollutants. Based on the model estimation, several policy implications for a sustainable land use pattern was discussed.

• Korean Institute of Interior Design Journal
• /
• no.40
• /
• pp.34-41
• /
• 2003

A purpose of this study is to comprehend a concept of 'Open Space', realization of a deconstruction, presented in his works, based on a deconstruction and surrealism Coop Himmelblau's philosophical contemplation and to look into how this concept is interpreted in spaces. A concept of 'Open Space' is functioned as not a singular meaning but a multiple meaning accompanied with other formative ingredient and contemplation, scientific paradigm and a realation with a surroundings. The concept of 'Open Space' in their architentural space is applied. First, the space openness that represents to the extreme a concept 'Open Space', as a 3D designing ways that demolishes a structure and outgrows an ex siting thinking pattern, represents multiple grade spaces and an anti-gravity space. In a material, using glass and iron provides a spacial transparency and through this, visibly liberal sense that an interior and exterior is felt as an unification. Second, an non-expressive box pattern repulses to a spatial rank nature, outgrowing an uniform geometrical system, through demolishing a geometrical system, an edge and fold pattern. This is, as an anti-gravity, dynamic a typical structure, outgrowing an orthogonal system, interpreted as a composite meaning without division in spatial area. Third, the collage is used to represent a complexity and pluralism in representing 'Open space'. The collage that forms a image through combining a fragmentary elements into being a space change, composite constitution and spatial amusement. Thus, It is worthwhile to study how the collage that forms a diverse shape will be developed making what impact as an age and surroundings changed. As we contemplate in former part, Coop Himmelblou has deployed 'deconstruction beyond deconstruction' realm as just their midterm concept in their works like the words 'The architecture must climb', the deconstructive architect. A studying for their works reflecting the 'Open Space' concept based on deconstruction must be lasted and this enables us to comprehend space concept containing an architecture and interior design.

• Journal of KIISE:Information Networking
• /
• v.35 no.5
• /
• pp.367-373
• /
• 2008

Recently, the NAT middlebox widely deployed in the home network environment prohibits DM operations from reaching user devices behind NAT. In this article, we focus on NAT issues to manage home network devices. Particularly, we discuss standardization efforts, and present our proposal to deploy DM services for VoIP and IPTV devices under NAT. By slightly changing behaviors of Simple Network Management Protocol (SNMP) Manager and Agent, and defining additional Management Objects (MOs) to gather NAT binding information, we could solve the NAT traversal problem under symmetric NAT. Moreover, we propose an enhanced method to search the UDP hole binding time of the NAT box. We applied our method to randomly selected 22 VoIP devices out of 194 NATed hosts in the real broadband network and have achieved 99% of the success ratio for exchanging SNMP request messages and 26% of enhancement for searching the UDP hole binding time.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.27 no.9C
• /
• pp.842-852
• /
• 2002

The fast advance in the VoIP technologies gives a rich opportunity to create different kind of VoIP applications such as IP telephony services. The application level call signaling protocols such as ITU-T H.323 and IETF SIP provide the communication functions of end-to-end call setup and release. Currently, there is a lot of H.323 based VoIP products in the market, however SIP is considered as a suitable protocol for supporting applications in IP environments, so SIP based VoIP products and services begin to appear. In this paper, firstly we present the characteristics of some possible SIP based applications and describe the design and implementation of a VoIP example service named PC-to-PC Internet telephony service using the developed SIP network components. The PC-to-PC Internet telephony service and User Agent are developed in MS window 98/2000 using visual C/C++, and Proxy server and Registrar in Linux 7.0 using C, respectively.

• Geotechnical Engineering
• /
• v.10 no.4
• /
• pp.67-82
• /
• 1994

In this study, a reinforced clay model based on the limit equilibrium of forces under direct shear was proposed to predict shear strength increase in clays induced by the steel inclusion. The model accounted for the effects of orientation of inclusion, length, bonding stress between clay and indclusion and passive soil resistance 1 induced by the inclusion movement, on the behavior of reinforced clays. In order to compare with the theoretical predictions, direct shear tests were performed using a direct shear apparatus formed of an open shear box. Also pull-out tests were conducted to determine the bonding stress between the inclusion and clay. From the experimental results, the increase or decrease in shear strength of reinforced clay samples was found to depend on the orientation of inclusionas well as water content of clay samples. From the comparison of theoretical predictions and experimental results, it was found that the theore tical model predicted reasonably well the influence of orientation of the inclusion as well as passive soil resistance induced by the inclusion movement on the mechanical behavior of reinforced clays.

• Journal of the Korean Geotechnical Society
• /
• v.15 no.4
• /
• pp.261-277
• /
• 1999

The horizontal and vertical earth pressures in backfill space which is narrowly excavated like ditch are affected by the share of ditch backfill space and the wall friction between excavated surface and backfill soil. In this paper, for the excavated surface the Handy's equation of a symmetric vertical case and the Kellogg's equation of a symmetric sloped one are modified to show the minor principal stress arch for the unsymmetrical excavated backfill space. Compared with the soil test box result, a similarity in magnitude and distribution of backfill earth pressure shows that the earth pressure has been observed. The backfill earth pressure in unsymmetrically sloped space has been shown twice as much as the one in vertically excavated space and also remarkable decline of arching for the former case. It is verified that the earth pressure equation should account the shape and size of backfill space to calculate the earth pressure for similar structure to the one handled in this study.