Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Overexpression of SOX15 Inhibits Proliferation of NT2/D1 Cells Derived from a Testicular Embryonal Cell Carcinoma

  • Yan, Hong-Tao (Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics, The University of Tokushima) ;
  • Shinka, Toshikatsu (Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics, The University of Tokushima) ;
  • Sato, Youichi (Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics, The University of Tokushima) ;
  • Yang, Xin-Jun (Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics, The University of Tokushima) ;
  • Chen, Gang (Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics, The University of Tokushima) ;
  • Sakamoto, Kozue (Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics, The University of Tokushima) ;
  • Kinoshita, Keigo (Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics, The University of Tokushima) ;
  • Aburatani, Hiroyuki (Genome Science Division, Center for Collaborative Research, University of Tokyo) ;
  • Nakahori, Yutaka (Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics, The University of Tokushima)
  • Received : 2007.01.28
  • Accepted : 2007.07.16
  • Published : 2007.12.31
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Abstract

SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were up-regulated in the SOX15-NT2/D1 cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.

Keywords

Acknowledgement

Supported by : Ministry of Health and Ministry of Health, Labour and Welfare

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